Restriction map for
pFN21AE2270
(Circular) (Six-base) MAPSORT of: pFN21AE2270.seq Check: 1331 from: 1 to: 5143
pFN21AE2270 5143 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2215 2215
Size: 5143
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2953 278
Size: 53 83 186 936 1417 2468
Fragments arranged by size:
2468 1417 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2486 1937
Size: 549 4594
Acc65I G'GTAC_C
Cuts at: 2464 2464
Size: 5143
AclI AA'CG_TT
Cuts at: 1563 3270 3643 1563
Size: 1707 373 3063
Fragments arranged by size:
3063 1707 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3206 4590 4858 1632
Size: 1574 1384 268 1917
Fragments arranged by size:
1917 1574 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4946
AflIII A'CryG_T
Cuts at: 1203 2202 2297 4042 5019 1203
Size: 999 95 1745 977 1327
Fragments arranged by size:
1745 1327 999 977 95
AgeI A'CCGG_T
Cuts at: 3974 3974
Size: 5143
AhdI GACnn_n'nnGTC
Cuts at: 3872 3872
Size: 5143
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5143
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5143
AlwNI CAG_nnn'CTG
Cuts at: 2886 4458 2886
Size: 1572 3571
ApaI G_GGCC'C
Cuts at: 2016 2016
Size: 5143
ApaLI G'TGCA_C
Cuts at: 2157 3200 4356 2157
Size: 1043 1156 2944
Fragments arranged by size:
2944 1156 1043
ApoI r'AATT_y
Cuts at: 1493 2452 2678 2830 4939 1493
Size: 959 226 152 2109 1697
Fragments arranged by size:
2109 1697 959 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3697 160
Size: 872 2665 1606
Fragments arranged by size:
2665 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5143
AvaI C'yCGr_G
Cuts at: 1870 1943 2468 1870
Size: 73 525 4545
Fragments arranged by size:
4545 525 73
BamHI G'GATC_C
Cuts at: 2473 2840 2473
Size: 367 4776
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2464 3919 618
Size: 325 217 627 677 1455 1842
Fragments arranged by size:
1842 1455 677 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2016 2072 2462 1391
Size: 625 56 390 4072
Fragments arranged by size:
4072 625 390 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2230 2264 3334 3368 1903
Size: 34 293 34 1070 34 3678
Fragments arranged by size:
3678 1070 293 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3035 4245 3035
Size: 1210 3933
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5143
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2048 3754 136
Size: 107 122 71 631 605 376 1706 1525
Fragments arranged by size:
1706 1525 631 605 376 122 107 71
BglII A'GATC_T
Cuts at: 2382 5138 2382
Size: 2756 2387
BlpI GC'TnA_GC
Cuts at: 2560 2560
Size: 5143
Bme1580I G_kGCm'C
Cuts at: 1790 2016 2161 2408 3204 4360 1790
Size: 226 145 247 796 1156 2573
Fragments arranged by size:
2573 1156 796 247 226 145
BmgBI CAC'GTC
Cuts at: 1318 2278 1318
Size: 960 4183
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2874 3832 442
Size: 416 53 83 399 24 1457 958 1753
Fragments arranged by size:
1753 1457 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2068 3803 1239
Size: 829 1735 2579
Fragments arranged by size:
2579 1735 829
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3268 4133 4431 3268
Size: 865 298 3980
Fragments arranged by size:
3980 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3806 882
Size: 617 2307 2219
Fragments arranged by size:
2307 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2298 2934 493
Size: 711 1094 636 2702
Fragments arranged by size:
2702 1094 711 636
BsaBI GATnn'nnATC
Cuts at: 5137 5137
Size: 5143
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2127 2439 2950 3332
Size: 53 83 186 936 594 312 511 382
Cuts at: 3332 3967 275
Size: 635 1451
Fragments arranged by size:
1451 936 635 594 511 382 312 186
83 53
BsaWI w'CCGG_w
Cuts at: 3576 3974 4248 4395 4806 3576
Size: 398 274 147 411 3913
Fragments arranged by size:
3913 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4918
Fragments arranged by size:
4918 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2156 1186
Size: 967 3 4173
Fragments arranged by size:
4173 967 3
BseYI C'CCAG_C
Cuts at: 2255 2284 2310 4346 4842 2255
Size: 29 26 2036 496 2556
Fragments arranged by size:
2556 2036 496 29 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4570
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2079 2593 3354 4382 1540
Size: 275 186 78 514 761 1028 2301
Fragments arranged by size:
2301 1028 761 514 275 186 78
BsiHKAI G_wGCw'C
Cuts at: 2161 3204 3289 4360 2161
Size: 1043 85 1071 2944
Fragments arranged by size:
2944 1071 1043 85
BsmI GAATG_Cn'
Cuts at: 2366 2654 2747 2366
Size: 288 93 4762
Fragments arranged by size:
4762 288 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2072 2161 2408 2462 3204 3289
Size: 399 226 56 89 247 54 742 85
Cuts at: 3289 4360 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 247 226 89 85
56 54
BspEI T'CCGG_A
Cuts at: 4806 4806
Size: 5143
BspHI T'CATG_A
Cuts at: 3032 4762 3032
Size: 1730 3413
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2215 2498 844
Size: 1371 283 3489
Fragments arranged by size:
3489 1371 283
BsrBI CCG'CTC
Cuts at: 1394 1823 3030 1394
Size: 429 1207 3507
Fragments arranged by size:
3507 1207 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3638 3812 65
Size: 1183 2390 174 1396
Fragments arranged by size:
2390 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2078 3787 3974 1700
Size: 110 268 1709 187 2869
Fragments arranged by size:
2869 1709 268 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5143
BssHII G'CGCG_C
Cuts at: 1925 2020 2217 2233 1925
Size: 95 197 16 4835
Fragments arranged by size:
4835 197 95 16
BssSI C'ACGA_G
Cuts at: 3203 4215 3203
Size: 1012 4131
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2230 794
Size: 1436 3707
BstBI TT'CG_AA
Cuts at: 3947 3947
Size: 5143
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5143
BstYI r'GATC_y
Cuts at: 1972 2382 2473 2840 3225 3242 4694 4780 4792
Size: 410 91 367 385 17 1452 86 12
Cuts at: 4792 5138 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 410 385 367 346 91 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 2073 2121 513
Size: 552 939 48 21 48 3535
Fragments arranged by size:
3535 939 552 48 48 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4404
Fragments arranged by size:
4404 683 56
BtsI GCAGTG_nn'
Cuts at: 2659 3453 3473 2659
Size: 794 20 4329
Fragments arranged by size:
4329 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5143
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5108
DraI TTT'AAA
Cuts at: 2447 2447
Size: 5143
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2171 4150 809
Size: 507 855 1979 1802
Fragments arranged by size:
1979 1802 855 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2040 2049
Size: 54 1425 50 275 18 177 33 9
Cuts at: 2049 2061 2076 2590 3479 5108 8
Size: 12 15 514 889 1629 43
Fragments arranged by size:
1629 1425 889 514 275 177 54 50
43 33 18 15 12 9
EagI C'GGCC_G
Cuts at: 1537 1812 2076 2590 1537
Size: 275 264 514 4090
Fragments arranged by size:
4090 514 275 264
EarI CTCTTCn'nnn_
Cuts at: 1353 3073 1353
Size: 1720 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3730 4104 4250 387
Size: 1064 138 2141 374 146 1280
Fragments arranged by size:
2141 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 3206 3803 4590 4858 1239
Size: 393 436 1138 597 787 268 1524
Fragments arranged by size:
1524 1138 787 597 436 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2013 2117 2013
Size: 104 5039
EcoRI G'AATT_C
Cuts at: 2452 2830 2452
Size: 378 4765
FspI TGC'GCA
Cuts at: 3649 3649
Size: 5143
HaeII r_GCGC'y
Cuts at: 1678 4290 1678
Size: 2612 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3784 3816 3858 3890 1373
Size: 32 135 32 2212 32 42 32 2626
Fragments arranged by size:
2626 2212 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2487 2733 669
Size: 988 281 549 246 3079
Fragments arranged by size:
3079 988 549 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5143
HpaI GTT'AAC
Cuts at: 2733 2733
Size: 5143
KpnI G_GTAC'C
Cuts at: 2468 2468
Size: 5143
MfeI C'AATT_G
Cuts at: 2742 2742
Size: 5143
MluI A'CGCG_T
Cuts at: 5019 5019
Size: 5143
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4257 4441 1305
Size: 2952 184 2007
Fragments arranged by size:
2952 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2042 10
Size: 54 1425 343 210 3111
Fragments arranged by size:
3111 1425 343 210 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3101 3460 3619 518
Size: 648 1935 359 159 2042
Fragments arranged by size:
2042 1935 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2075 2559 3236 4384 4629 1606
Size: 423 46 484 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 484 423 245 46
NaeI GCC'GGC
Cuts at: 2080 2080
Size: 5143
NcoI C'CATG_G
Cuts at: 513 1065 2004 2121 513
Size: 552 939 117 3535
Fragments arranged by size:
3535 939 552 117
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5143
NgoMIV G'CCGG_C
Cuts at: 2078 2078
Size: 5143
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5143
NotI GC'GGCC_GC
Cuts at: 2590 2590
Size: 5143
NspI r_CATG'y
Cuts at: 1132 1229 2206 2501 4046 1132
Size: 97 977 295 1545 2229
Fragments arranged by size:
2229 1545 977 295 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5143
PciI A'CATG_T
Cuts at: 2202 4042 2202
Size: 1840 3303
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5143
PmeI GTTT'AAAC
Cuts at: 2447 2447
Size: 5143
PmlI CAC'GTG
Cuts at: 2298 2298
Size: 5143
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3212 3244 4725 4757 3212
Size: 32 1481 32 3598
Fragments arranged by size:
3598 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5143
PsiI TTA'TAA
Cuts at: 2713 2713
Size: 5143
PspOMI G'GGCC_C
Cuts at: 2012 2012
Size: 5143
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2284 2316 2284
Size: 32 5111
PstI C_TGCA'G
Cuts at: 830 2226 2495 830
Size: 1396 269 3478
Fragments arranged by size:
3478 1396 269
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5143
SacII CC_GC'GG
Cuts at: 2076 2076
Size: 5143
SalI G'TCGA_C
Cuts at: 1936 2485 1936
Size: 549 4594
SbfI CC_TGCA'GG
Cuts at: 2495 2495
Size: 5143
ScaI AGT'ACT
Cuts at: 3391 5103 3391
Size: 1712 3431
SfcI C'TryA_G
Cuts at: 826 1045 2222 2491 3626 4307 4498 826
Size: 219 1177 269 1135 681 191 1471
Fragments arranged by size:
1471 1177 1135 681 269 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2048 2048
Size: 5143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5143
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5143
SmaI CCC'GGG
Cuts at: 1872 2470 1872
Size: 598 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3247 4148 4410 820
Size: 197 926 1304 901 262 1553
Fragments arranged by size:
1553 1304 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2934 493
Size: 2441 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5143
SphI G_CATG'C
Cuts at: 1132 2501 1132
Size: 1369 3774
SspI AAT'ATT
Cuts at: 5 52 3067 5
Size: 47 3015 2081
Fragments arranged by size:
3015 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5143
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 2396 2582 513
Size: 552 939 117 275 186 3074
Fragments arranged by size:
3074 939 552 275 186 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3170 3187 3340 3525 3970 325
Size: 1255 1590 17 153 185 445 1498
Fragments arranged by size:
1590 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3389 5101
Size: 274 80 33 51 1443 204 1208 1712
Cuts at: 5101 96
Size: 138
Fragments arranged by size:
1712 1443 1208 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5103 5135 5103
Size: 32 5111
Tth111I GACn'n_nGTC
Cuts at: 1316 2416 1316
Size: 1100 4043
XbaI T'CTAG_A
Cuts at: 2479 2479
Size: 5143
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2050 2050
Size: 5143
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5143
XmaI C'CCGG_G
Cuts at: 1870 2468 1870
Size: 598 4545
XmnI GAAnn'nnTTC
Cuts at: 2267 2366 3272 2267
Size: 99 906 4138
Fragments arranged by size:
4138 906 99
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2951 276
Size: 53 83 186 936 1417 2468
Fragments arranged by size:
2468 1417 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PsrI PstI PvuI SacII SalI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PpuMI PspXI PvuII RsrII
SacI SanDI SapI SexAI SfoI SrfI SwaI