Restriction map for
pFN21AE2266
(Circular) (Six-base) MAPSORT of: pFN21AE2266.seq Check: 2383 from: 1 to: 7261
pFN21AE2266 7261 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5071 278
Size: 53 83 186 936 3535 2468
Fragments arranged by size:
3535 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4604 1937
Size: 2667 4594
Acc65I G'GTAC_C
Cuts at: 4582 4582
Size: 7261
AclI AA'CG_TT
Cuts at: 1563 5388 5761 1563
Size: 3825 373 3063
Fragments arranged by size:
3825 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2597 2617 2676 3210 3339 3953 5324 6708
Size: 965 20 59 534 129 614 1371 1384
Cuts at: 6708 6976 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1371 965 614 534 268 129
59 20
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7064
AflIII A'CryG_T
Cuts at: 1203 2281 2879 3212 4286 6160 7137 1203
Size: 1078 598 333 1074 1874 977 1327
Fragments arranged by size:
1874 1327 1078 1074 977 598 333
AgeI A'CCGG_T
Cuts at: 6092 6092
Size: 7261
AhdI GACnn_n'nnGTC
Cuts at: 5990 5990
Size: 7261
AleI CACnn'nnGTG
Cuts at: 1166 2063 3011 4268 1166
Size: 897 948 1257 4159
Fragments arranged by size:
4159 1257 948 897
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7261
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4330 4362 4330
Size: 32 7229
AlwNI CAG_nnn'CTG
Cuts at: 3244 4138 5004 6576 3244
Size: 894 866 1572 3929
Fragments arranged by size:
3929 1572 894 866
ApaLI G'TGCA_C
Cuts at: 5318 6474 5318
Size: 1156 6105
ApoI r'AATT_y
Cuts at: 1493 2706 2828 4570 4796 4948 7057 1493
Size: 1213 122 1742 226 152 2109 1697
Fragments arranged by size:
2109 1742 1697 1213 226 152 122
AseI AT'TA_AT
Cuts at: 160 1032 3368 5815 160
Size: 872 2336 2447 1606
Fragments arranged by size:
2447 2336 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7261
AvaI C'yCGr_G
Cuts at: 1870 1943 2019 4586 1870
Size: 73 76 2567 4545
Fragments arranged by size:
4545 2567 76 73
BamHI G'GATC_C
Cuts at: 4591 4958 4591
Size: 367 6894
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2480 2996 4582 6037 618
Size: 325 217 627 693 516 1586 1455 1842
Fragments arranged by size:
1842 1586 1455 693 627 516 325 217
BanII G_rGCy'C
Cuts at: 1391 2020 2904 3141 3707 4580 1391
Size: 629 884 237 566 873 4072
Fragments arranged by size:
4072 884 873 629 566 237
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2611 3677 928
Size: 971 712 1066 4512
Fragments arranged by size:
4512 1066 971 712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5452 5486 1903
Size: 34 3515 34 3678
Fragments arranged by size:
3678 3515 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3974 5153 6363 3974
Size: 1179 1210 4872
Fragments arranged by size:
4872 1210 1179
BclI T'GATC_A
Cuts at: 1548 4314 1548
Size: 2766 4495
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5872 136
Size: 107 122 71 631 605 4200 1525
Fragments arranged by size:
4200 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2096 7256 2096
Size: 5160 2101
BlpI GC'TnA_GC
Cuts at: 3114 4139 4678 3114
Size: 1025 539 5697
Fragments arranged by size:
5697 1025 539
Bme1580I G_kGCm'C
Cuts at: 1790 5322 6478 1790
Size: 3532 1156 2573
Fragments arranged by size:
3532 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2880 3215 3901 1318
Size: 1562 335 686 4678
Fragments arranged by size:
4678 1562 686 335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2046 3710 4521
Size: 416 53 83 399 24 629 1664 811
Cuts at: 4521 4992 5950 442
Size: 471 958 1753
Fragments arranged by size:
1753 1664 958 811 629 471 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4038 5921 1239
Size: 2799 1883 2579
Fragments arranged by size:
2799 2579 1883
Bpu10I CC'TnA_GC
Cuts at: 3135 3135
Size: 7261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3541 4159 5386 6251 6549 3541
Size: 618 1227 865 298 4253
Fragments arranged by size:
4253 1227 865 618 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3293 5924 882
Size: 617 1794 2631 2219
Fragments arranged by size:
2631 2219 1794 617
BsaAI yAC'GTr
Cuts at: 493 1204 4289 5052 493
Size: 711 3085 763 2702
Fragments arranged by size:
3085 2702 763 711
BsaBI GATnn'nnATC
Cuts at: 2567 3773 7255 2567
Size: 1206 3482 2573
Fragments arranged by size:
3482 2573 1206
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5068 5450 6085 275
Size: 53 83 186 936 3535 382 635 1451
Fragments arranged by size:
3535 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2808 5694 6092 6366 6513 6924 2808
Size: 2886 398 274 147 411 3145
Fragments arranged by size:
3145 2886 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7036
Fragments arranged by size:
7036 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7261
BseYI C'CCAG_C
Cuts at: 2712 4462 6464 6960 2712
Size: 1750 2002 496 3013
Fragments arranged by size:
3013 2002 1750 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6688
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4711 5472 6500 1540
Size: 275 186 2710 761 1028 2301
Fragments arranged by size:
2710 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3141 3707 4024 5322 5407 6478 3141
Size: 566 317 1298 85 1071 3924
Fragments arranged by size:
3924 1298 1071 566 317 85
BsmI GAATG_Cn'
Cuts at: 2471 3651 4512 4772 4865 2471
Size: 1180 861 260 93 4867
Fragments arranged by size:
4867 1180 861 260 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2810 3221 2810
Size: 411 6850
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2904 3141 3707 4024 4580 5322
Size: 399 230 884 237 566 317 556 742
Cuts at: 5322 5407 6478 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 884 742 566 556 399 317
237 230 85
BspEI T'CCGG_A
Cuts at: 2808 6924 2808
Size: 4116 3145
BspHI T'CATG_A
Cuts at: 2832 4011 4048 5150 6880 2832
Size: 1179 37 1102 1730 3213
Fragments arranged by size:
3213 1730 1179 1102 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3524 4616 844
Size: 2680 1092 3489
Fragments arranged by size:
3489 2680 1092
BsrBI CCG'CTC
Cuts at: 1394 1823 5148 1394
Size: 429 3325 3507
Fragments arranged by size:
3507 3325 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2087 2140 5756 5930 65
Size: 1183 839 53 3616 174 1396
Fragments arranged by size:
3616 1396 1183 839 174 53
BsrFI r'CCGG_y
Cuts at: 1700 1810 3231 5905 6092 1700
Size: 110 1421 2674 187 2869
Fragments arranged by size:
2869 2674 1421 187 110
BsrGI T'GTAC_A
Cuts at: 96 4243 96
Size: 4147 3114
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7261
BssSI C'ACGA_G
Cuts at: 5321 6333 5321
Size: 1012 6249
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7261
BstBI TT'CG_AA
Cuts at: 3424 6065 3424
Size: 2641 4620
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7261
BstXI CCAn_nnnn'nTGG
Cuts at: 2065 3256 2065
Size: 1191 6070
BstYI r'GATC_y
Cuts at: 1972 2096 2157 2640 4591 4958 5343 5360 6812
Size: 124 61 483 1951 367 385 17 1452
Cuts at: 6812 6898 6910 7256 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1951 1452 483 385 367 346 124
86 61 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3540 513
Size: 552 2475 4234
Fragments arranged by size:
4234 2475 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2023 487
Size: 683 56 797 5725
Fragments arranged by size:
5725 797 683 56
BtsI GCAGTG_nn'
Cuts at: 4150 4777 5571 5591 4150
Size: 627 794 20 5820
Fragments arranged by size:
5820 794 627 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7261
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7226
DraI TTT'AAA
Cuts at: 3441 3846 4565 3441
Size: 405 719 6137
Fragments arranged by size:
6137 719 405
DraIII CAC_nnn'GTG
Cuts at: 3948 3948
Size: 7261
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3716 6268 809
Size: 507 2400 2552 1802
Fragments arranged by size:
2552 2400 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3246 3340 4442
Size: 54 1425 50 275 18 1416 94 1102
Cuts at: 4442 4708 5597 7226 8
Size: 266 889 1629 43
Fragments arranged by size:
1629 1425 1416 1102 889 275 266 94
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4708 1537
Size: 275 2896 4090
Fragments arranged by size:
4090 2896 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2008 2573 3086 3520 4556 5191 1353
Size: 655 565 513 434 1036 635 3423
Fragments arranged by size:
3423 1036 655 635 565 513 434
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5848 6222 6368 387
Size: 1064 138 4259 374 146 1280
Fragments arranged by size:
4259 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3139 3705 3139
Size: 566 6695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2597 2617 2676 3210 3339 3953 4038
Size: 393 965 20 59 534 129 614 85
Cuts at: 4038 5324 5921 6708 6976 1239
Size: 1286 597 787 268 1524
Fragments arranged by size:
1524 1286 965 787 614 597 534 393
268 129 85 59 20
EcoRI G'AATT_C
Cuts at: 2828 4570 4948 2828
Size: 1742 378 5141
Fragments arranged by size:
5141 1742 378
EcoRV GAT'ATC
Cuts at: 2184 3330 2184
Size: 1146 6115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4510 4510
Size: 7261
FspI TGC'GCA
Cuts at: 5767 5767
Size: 7261
HaeII r_GCGC'y
Cuts at: 1678 3244 6408 1678
Size: 1566 3164 2531
Fragments arranged by size:
3164 2531 1566
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2393 2425 2576 2608 5902
Size: 32 135 32 821 32 151 32 3294
Cuts at: 5902 5934 5976 6008 1373
Size: 32 42 32 2626
Fragments arranged by size:
3294 2626 821 151 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3389 3931 4605 4851 669
Size: 988 281 1451 542 674 246 3079
Fragments arranged by size:
3079 1451 988 674 542 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7261
HpaI GTT'AAC
Cuts at: 3931 4851 3931
Size: 920 6341
KpnI G_GTAC'C
Cuts at: 4586 4586
Size: 7261
MfeI C'AATT_G
Cuts at: 4860 4860
Size: 7261
MluI A'CGCG_T
Cuts at: 7137 7137
Size: 7261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2481 3892 6375 6559 1305
Size: 1176 1411 2483 184 2007
Fragments arranged by size:
2483 2007 1411 1176 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3248 10
Size: 54 1425 343 1416 4023
Fragments arranged by size:
4023 1425 1416 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2063 2750 3011 3767 4268 5219 5578
Size: 648 897 687 261 756 501 951 359
Cuts at: 5578 5737 518
Size: 159 2042
Fragments arranged by size:
2042 951 897 756 687 648 501 359
261 159
MspA1I CmG'CkG
Cuts at: 1606 3187 3593 3640 3960 4102 4135 4677 5354
Size: 1581 406 47 320 142 33 542 677
Cuts at: 5354 6502 6747 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1581 1148 677 542 406 320 245
142 47 33
NaeI GCC'GGC
Cuts at: 3233 3233
Size: 7261
NcoI C'CATG_G
Cuts at: 513 1065 3540 513
Size: 552 2475 4234
Fragments arranged by size:
4234 2475 552
NdeI CA'TA_TG
Cuts at: 387 3383 4398 387
Size: 2996 1015 3250
Fragments arranged by size:
3250 2996 1015
NgoMIV G'CCGG_C
Cuts at: 3231 3231
Size: 7261
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7261
NotI GC'GGCC_GC
Cuts at: 4708 4708
Size: 7261
NspI r_CATG'y
Cuts at: 1132 1229 2285 4213 4619 6164 1132
Size: 97 1056 1928 406 1545 2229
Fragments arranged by size:
2229 1928 1545 1056 406 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7261
PciI A'CATG_T
Cuts at: 2281 6160 2281
Size: 3879 3382
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7261
PmeI GTTT'AAAC
Cuts at: 4565 4565
Size: 7261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5330 5362 6843 6875 5330
Size: 32 1481 32 5716
Fragments arranged by size:
5716 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7261
PsiI TTA'TAA
Cuts at: 4831 4831
Size: 7261
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2287 2319 2287
Size: 32 7229
PstI C_TGCA'G
Cuts at: 830 4613 830
Size: 3783 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7261
PvuII CAG'CTG
Cuts at: 3187 3593 3640 4102 4135 3187
Size: 406 47 462 33 6313
Fragments arranged by size:
6313 462 406 47 33
SacI G_AGCT'C
Cuts at: 3141 3707 3141
Size: 566 6695
SalI G'TCGA_C
Cuts at: 1936 4603 1936
Size: 2667 4594
SapI GCTCTTCn'nnn_
Cuts at: 2008 2008
Size: 7261
SbfI CC_TGCA'GG
Cuts at: 4613 4613
Size: 7261
ScaI AGT'ACT
Cuts at: 5509 7221 5509
Size: 1712 5549
SexAI A'CCwGG_T
Cuts at: 3461 3944 4557 3461
Size: 483 613 6165
Fragments arranged by size:
6165 613 483
SfcI C'TryA_G
Cuts at: 826 1045 4609 5744 6425 6616 826
Size: 219 3564 1135 681 191 1471
Fragments arranged by size:
3564 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7261
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7261
SmaI CCC'GGG
Cuts at: 1872 4588 1872
Size: 2716 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3520 4174 5365 6266 6528 820
Size: 197 926 1577 654 1191 901 262 1553
Fragments arranged by size:
1577 1553 1191 926 901 654 262 197
SnaBI TAC'GTA
Cuts at: 493 5052 493
Size: 4559 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7261
SphI G_CATG'C
Cuts at: 1132 4213 4619 1132
Size: 3081 406 3774
Fragments arranged by size:
3774 3081 406
SspI AAT'ATT
Cuts at: 5 52 3366 3698 5185 5
Size: 47 3314 332 1487 2081
Fragments arranged by size:
3314 2081 1487 332 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7261
StyI C'CwwG_G
Cuts at: 513 1065 3414 3540 4700 513
Size: 552 2349 126 1160 3074
Fragments arranged by size:
3074 2349 1160 552 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2888 5288 5305 5458 5643 6088 325
Size: 1255 1308 2400 17 153 185 445 1498
Fragments arranged by size:
2400 1498 1308 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3577 4041 4243
Size: 274 80 33 51 1443 1600 464 202
Cuts at: 4243 4431 5507 7219 96
Size: 188 1076 1712 138
Fragments arranged by size:
1712 1600 1443 1076 464 274 202 188
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2304 2336 2708 2740 3537 3569 7221 7253 2304
Size: 32 372 32 797 32 3652 32 2312
Fragments arranged by size:
3652 2312 797 372 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7261
XbaI T'CTAG_A
Cuts at: 2411 4597 2411
Size: 2186 5075
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2272 2272
Size: 7261
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7261
XmaI C'CCGG_G
Cuts at: 1870 4586 1870
Size: 2716 4545
XmnI GAAnn'nnTTC
Cuts at: 4007 5390 4007
Size: 1383 5878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5069 276
Size: 53 83 186 936 3535 2468
Fragments arranged by size:
3535 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I EcoNI EcoO109I FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI RsrII SacII SanDI SfiI SfoI
SrfI SwaI