Restriction map for
pFN21AE2260
(Circular) (Six-base) MAPSORT of: pFN21AE2260.seq Check: 6228 from: 1 to: 6256
pFN21AE2260 6256 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2130 2142 2130
Size: 12 6244
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2262 3441 3599 1937
Size: 325 1179 158 4594
Fragments arranged by size:
4594 1179 325 158
Acc65I G'GTAC_C
Cuts at: 3577 3577
Size: 6256
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2950 3477 3480 4319 5703 5971 1632
Size: 1318 527 3 839 1384 268 1917
Fragments arranged by size:
1917 1384 1318 839 527 268 3
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6059
AflIII A'CryG_T
Cuts at: 1203 5155 6132 1203
Size: 3952 977 1327
Fragments arranged by size:
3952 1327 977
AgeI A'CCGG_T
Cuts at: 5087 5087
Size: 6256
AhdI GACnn_n'nnGTC
Cuts at: 4985 4985
Size: 6256
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2840 1184
Size: 1656 4600
AlwNI CAG_nnn'CTG
Cuts at: 2122 2134 3217 3485 3999 5571 2122
Size: 12 1083 268 514 1572 2807
Fragments arranged by size:
2807 1572 1083 514 268 12
ApaLI G'TGCA_C
Cuts at: 3089 4313 5469 3089
Size: 1224 1156 3876
Fragments arranged by size:
3876 1224 1156
ApoI r'AATT_y
Cuts at: 1493 2192 2416 3565 3791 3943 6052 1493
Size: 699 224 1149 226 152 2109 1697
Fragments arranged by size:
2109 1697 1149 699 226 224 152
AseI AT'TA_AT
Cuts at: 160 1032 4810 160
Size: 872 3778 1606
Fragments arranged by size:
3778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 3581 1870
Size: 73 1638 4545
Fragments arranged by size:
4545 1638 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3028 3061 3028
Size: 33 6223
BamHI G'GATC_C
Cuts at: 3586 3953 3586
Size: 367 5889
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3577 5032 618
Size: 325 217 627 1790 1455 1842
Fragments arranged by size:
1842 1790 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3575 1391
Size: 2184 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5285
BbvCI CC'TCA_GC
Cuts at: 3531 3531
Size: 6256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2964 2998 3167 3201 4447
Size: 34 50 34 943 34 169 34 1246
Cuts at: 4447 4481 1903
Size: 34 3678
Fragments arranged by size:
3678 1246 943 169 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2908 4148 5358 2908
Size: 1240 1210 3806
Fragments arranged by size:
3806 1240 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6256
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3127 3279 4867
Size: 107 122 71 631 605 1455 152 1588
Cuts at: 4867 136
Size: 1525
Fragments arranged by size:
1588 1525 1455 631 605 152 122 107
71
BglII A'GATC_T
Cuts at: 6251 6251
Size: 6256
BlpI GC'TnA_GC
Cuts at: 3673 3673
Size: 6256
Bme1580I G_kGCm'C
Cuts at: 1790 2777 2974 3093 4317 5473 1790
Size: 987 197 119 1224 1156 2573
Fragments arranged by size:
2573 1224 1156 987 197 119
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6256
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3378 3473 3987
Size: 416 53 83 399 24 1961 95 514
Cuts at: 3987 4945 442
Size: 958 1753
Fragments arranged by size:
1961 1753 958 514 416 399 95 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6256
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2588 2588
Size: 6256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2885 4916 1239
Size: 1646 2031 2579
Fragments arranged by size:
2579 2031 1646
Bpu10I CC'TnA_GC
Cuts at: 3486 3531 3486
Size: 45 6211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2204 4381 5246 5544 2204
Size: 2177 865 298 2916
Fragments arranged by size:
2916 2177 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4919 882
Size: 617 3420 2219
Fragments arranged by size:
3420 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3400 4047 493
Size: 711 2196 647 2702
Fragments arranged by size:
2702 2196 711 647
BsaBI GATnn'nnATC
Cuts at: 6250 6250
Size: 6256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4063 4445 5080 275
Size: 53 83 186 936 2530 382 635 1451
Fragments arranged by size:
2530 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4689 5087 5361 5508 5919 4689
Size: 398 274 147 411 5026
Fragments arranged by size:
5026 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 1178
Size: 30 165 30 624 30 5377
Fragments arranged by size:
5377 624 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 2168 2183 2942 3347 3548 1186
Size: 979 3 15 759 405 201 3894
Fragments arranged by size:
3894 979 759 405 201 15 3
BseYI C'CCAG_C
Cuts at: 5459 5955 5459
Size: 496 5760
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2521 2944 1163
Size: 573 785 423 4475
Fragments arranged by size:
4475 785 573 423
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3706 4467 5495 1540
Size: 275 186 1705 761 1028 2301
Fragments arranged by size:
2301 1705 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2084 2409 2571 3093 4317 4402 5473 2084
Size: 325 162 522 1224 85 1071 2867
Fragments arranged by size:
2867 1224 1071 522 325 162 85
BsmI GAATG_Cn'
Cuts at: 3767 3860 3767
Size: 93 6163
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2409 2571 2777 2974 3093 3575
Size: 399 294 325 162 206 197 119 482
Cuts at: 3575 4317 4402 5473 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 482 399 325 294 206
197 162 119 85
BspEI T'CCGG_A
Cuts at: 5919 5919
Size: 6256
BspHI T'CATG_A
Cuts at: 4145 5875 4145
Size: 1730 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2130 2142 2543 2801 3611 844
Size: 1286 12 401 258 810 3489
Fragments arranged by size:
3489 1286 810 401 258 12
BsrBI CCG'CTC
Cuts at: 1394 1823 2728 4143 1394
Size: 429 905 1415 3507
Fragments arranged by size:
3507 1415 905 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4751 4925 65
Size: 1183 3503 174 1396
Fragments arranged by size:
3503 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2847 4900 5087 1700
Size: 110 1037 2053 187 2869
Fragments arranged by size:
2869 2053 1037 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6256
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6256
BssSI C'ACGA_G
Cuts at: 4316 5328 4316
Size: 1012 5244
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2134 2311 3220 794
Size: 1340 177 909 3830
Fragments arranged by size:
3830 1340 909 177
BstBI TT'CG_AA
Cuts at: 5060 5060
Size: 6256
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6256
BstYI r'GATC_y
Cuts at: 1972 3543 3586 3953 4338 4355 5807 5893 5905
Size: 1571 43 367 385 17 1452 86 12
Cuts at: 5905 6251 1972
Size: 346 1977
Fragments arranged by size:
1977 1571 1452 385 367 346 86 43
17 12
Bsu36I CC'TnA_GG
Cuts at: 3124 3124
Size: 6256
BtgI C'CryG_G
Cuts at: 513 1065 2088 513
Size: 552 1023 4681
Fragments arranged by size:
4681 1023 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5517
Fragments arranged by size:
5517 683 56
BtsI GCAGTG_nn'
Cuts at: 2269 3772 4566 4586 2269
Size: 1503 794 20 3939
Fragments arranged by size:
3939 1503 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6221
DraI TTT'AAA
Cuts at: 2857 3560 2857
Size: 703 5553
DraIII CAC_nnn'GTG
Cuts at: 3403 3403
Size: 6256
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3703 4592 6221
Size: 54 1425 50 275 18 1873 889 1629
Cuts at: 6221 8
Size: 43
Fragments arranged by size:
1873 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3703 1537
Size: 275 1891 4090
Fragments arranged by size:
4090 1891 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 2160 3318 3324 4186 1353
Size: 801 6 1158 6 862 3423
Fragments arranged by size:
3423 1158 862 801 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2243 4843 5217 5363 387
Size: 1064 138 654 2600 374 146 1280
Fragments arranged by size:
2600 1280 1064 654 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2885 2950 3477 3480 4319 4916 5703
Size: 393 1253 65 527 3 839 597 787
Cuts at: 5703 5971 1239
Size: 268 1524
Fragments arranged by size:
1524 1253 839 787 597 527 393 268
65 3
EcoO109I rG'GnC_Cy
Cuts at: 2064 2064
Size: 6256
EcoRI G'AATT_C
Cuts at: 2192 2416 3565 3943 2192
Size: 224 1149 378 4505
Fragments arranged by size:
4505 1149 378 224
EcoRV GAT'ATC
Cuts at: 2377 2377
Size: 6256
FspI TGC'GCA
Cuts at: 2750 4762 2750
Size: 2012 4244
HaeII r_GCGC'y
Cuts at: 1678 5403 1678
Size: 3725 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 4897 4929 4971
Size: 32 135 32 476 32 2817 32 42
Cuts at: 4971 5003 1373
Size: 32 2626
Fragments arranged by size:
2817 2626 476 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3600 3846 669
Size: 988 281 1662 246 3079
Fragments arranged by size:
3079 1662 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3515 748
Size: 2767 3489
HpaI GTT'AAC
Cuts at: 3846 3846
Size: 6256
KpnI G_GTAC'C
Cuts at: 3581 3581
Size: 6256
MfeI C'AATT_G
Cuts at: 3855 3855
Size: 6256
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5370 5554 1305
Size: 4065 184 2007
Fragments arranged by size:
4065 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4434
Fragments arranged by size:
4434 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2136 3051 3159 3398 4214 4573 4732
Size: 648 970 915 108 239 816 359 159
Cuts at: 4732 518
Size: 2042
Fragments arranged by size:
2042 970 915 816 648 359 239 159
108
MspA1I CmG'CkG
Cuts at: 1606 2800 2880 3181 3672 4349 5497 5742 1606
Size: 1194 80 301 491 677 1148 245 2120
Fragments arranged by size:
2120 1194 1148 677 491 301 245 80
NcoI C'CATG_G
Cuts at: 513 1065 2088 513
Size: 552 1023 4681
Fragments arranged by size:
4681 1023 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6256
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6256
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NspI r_CATG'y
Cuts at: 1132 1229 2111 2453 2626 2749 2827 3614 5159
Size: 97 882 342 173 123 78 787 1545
Cuts at: 5159 1132
Size: 2229
Fragments arranged by size:
2229 1545 882 787 342 173 123 97
78
PasI CC'CwG_GG
Cuts at: 1348 3464 1348
Size: 2116 4140
PciI A'CATG_T
Cuts at: 5155 5155
Size: 6256
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6256
PfoI T'CCnGG_A
Cuts at: 2343 2343
Size: 6256
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PmlI CAC'GTG
Cuts at: 3400 3400
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 5838 5870 4325
Size: 32 1481 32 4711
Fragments arranged by size:
4711 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2064 2064
Size: 6256
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 3826 3826
Size: 6256
PstI C_TGCA'G
Cuts at: 830 2127 2307 3180 3279 3608 830
Size: 1297 180 873 99 329 3478
Fragments arranged by size:
3478 1297 873 329 180 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
PvuII CAG'CTG
Cuts at: 2880 3181 2880
Size: 301 5955
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SbfI CC_TGCA'GG
Cuts at: 3279 3608 3279
Size: 329 5927
ScaI AGT'ACT
Cuts at: 4504 6216 4504
Size: 1712 4544
SfcI C'TryA_G
Cuts at: 826 1045 2048 2123 2303 2875 2938 3176 3275
Size: 219 1003 75 180 572 63 238 99
Cuts at: 3275 3604 4739 5420 5611 826
Size: 329 1135 681 191 1471
Fragments arranged by size:
1471 1135 1003 681 572 329 238 219
191 180 99 75 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 3583 1872
Size: 1711 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2183 4360 5261 5523 820
Size: 197 926 240 2177 901 262 1553
Fragments arranged by size:
2177 1553 926 901 262 240 197
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6256
SphI G_CATG'C
Cuts at: 1132 3614 1132
Size: 2482 3774
SspI AAT'ATT
Cuts at: 5 52 4180 5
Size: 47 4128 2081
Fragments arranged by size:
4128 2081 47
StuI AGG'CCT
Cuts at: 1517 2309 1517
Size: 792 5464
StyI C'CwwG_G
Cuts at: 513 1065 2088 2329 3032 3421 3695 513
Size: 552 1023 241 703 389 274 3074
Fragments arranged by size:
3074 1023 703 552 389 274 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4283 4300 4453 4638 5083 325
Size: 1255 2703 17 153 185 445 1498
Fragments arranged by size:
2703 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3010 4502 6214
Size: 274 80 33 51 1443 1033 1492 1712
Cuts at: 6214 96
Size: 138
Fragments arranged by size:
1712 1492 1443 1033 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2027 2059 6216 6248 2027
Size: 32 4157 32 2035
Fragments arranged by size:
4157 2035 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6256
XbaI T'CTAG_A
Cuts at: 3289 3592 3289
Size: 303 5953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2327 2327
Size: 6256
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6256
XmaI C'CCGG_G
Cuts at: 1870 3581 1870
Size: 1711 4545
XmnI GAAnn'nnTTC
Cuts at: 2420 3516 4385 2420
Size: 1096 869 4291
Fragments arranged by size:
4291 1096 869
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I EcoICRI EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI