Restriction map for
pFN21AE2247
(Circular) (Six-base) MAPSORT of: pFN21AE2247.seq Check: 1024 from: 1 to: 7381
pFN21AE2247 7381 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3112 4023 3112
Size: 911 6470
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5191 278
Size: 53 83 186 936 3655 2468
Fragments arranged by size:
3655 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4724 1937
Size: 2787 4594
Acc65I G'GTAC_C
Cuts at: 4702 4702
Size: 7381
AclI AA'CG_TT
Cuts at: 1563 5508 5881 1563
Size: 3945 373 3063
Fragments arranged by size:
3945 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2213 2421 5444 6828 7096 1632
Size: 581 208 3023 1384 268 1917
Fragments arranged by size:
3023 1917 1384 581 268 208
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7184
AflIII A'CryG_T
Cuts at: 1203 2727 4641 6280 7257 1203
Size: 1524 1914 1639 977 1327
Fragments arranged by size:
1914 1639 1524 1327 977
AgeI A'CCGG_T
Cuts at: 6212 6212
Size: 7381
AhdI GACnn_n'nnGTC
Cuts at: 4450 4642 6110 4450
Size: 192 1468 5721
Fragments arranged by size:
5721 1468 192
AleI CACnn'nnGTG
Cuts at: 1166 4143 1166
Size: 2977 4404
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7381
AlwNI CAG_nnn'CTG
Cuts at: 2941 3643 4210 5124 6696 2941
Size: 702 567 914 1572 3626
Fragments arranged by size:
3626 1572 914 702 567
ApaI G_GGCC'C
Cuts at: 3469 3936 3469
Size: 467 6914
ApaLI G'TGCA_C
Cuts at: 2264 3617 5438 6594 2264
Size: 1353 1821 1156 3051
Fragments arranged by size:
3051 1821 1353 1156
ApoI r'AATT_y
Cuts at: 1493 4376 4690 4916 5068 7177 1493
Size: 2883 314 226 152 2109 1697
Fragments arranged by size:
2883 2109 1697 314 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5935 160
Size: 872 4903 1606
Fragments arranged by size:
4903 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7381
AvaI C'yCGr_G
Cuts at: 1870 1943 2567 2909 4215 4307 4706 1870
Size: 73 624 342 1306 92 399 4545
Fragments arranged by size:
4545 1306 624 399 342 92 73
BamHI G'GATC_C
Cuts at: 2871 3939 4711 5078 2871
Size: 1068 772 367 5174
Fragments arranged by size:
5174 1068 772 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2905 2964 3767 3835 4702
Size: 325 217 627 1118 59 803 68 867
Cuts at: 4702 6157 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1118 867 803 627 325 217
68 59
BanII G_rGCy'C
Cuts at: 1391 2761 3387 3469 3916 3936 4700 1391
Size: 1370 626 82 447 20 764 4072
Fragments arranged by size:
4072 1370 764 626 447 82 20
BbeI G_GCGC'C
Cuts at: 2968 2968
Size: 7381
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2685 2841 928
Size: 971 786 156 5468
Fragments arranged by size:
5468 971 786 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5572 5606 1903
Size: 34 3635 34 3678
Fragments arranged by size:
3678 3635 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5273 6483 5273
Size: 1210 6171
BclI T'GATC_A
Cuts at: 1548 4060 1548
Size: 2512 4869
BfrBI ATG'CAT
Cuts at: 2923 3985 2923
Size: 1062 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2765 3554 5992
Size: 107 122 71 631 605 1093 789 2438
Cuts at: 5992 136
Size: 1525
Fragments arranged by size:
2438 1525 1093 789 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2271 3438 7376 2271
Size: 1167 3938 2276
Fragments arranged by size:
3938 2276 1167
BlpI GC'TnA_GC
Cuts at: 3044 4007 4798 3044
Size: 963 791 5627
Fragments arranged by size:
5627 963 791
Bme1580I G_kGCm'C
Cuts at: 1790 2268 2910 3469 3621 3770 3936 5442 6598
Size: 478 642 559 152 149 166 1506 1156
Cuts at: 6598 1790
Size: 2573
Fragments arranged by size:
2573 1506 1156 642 559 478 166 152
149
BmgBI CAC'GTC
Cuts at: 1318 2635 3754 1318
Size: 1317 1119 4945
Fragments arranged by size:
4945 1317 1119
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2068 3408 4310
Size: 416 53 83 399 24 651 1340 902
Cuts at: 4310 5112 6070 442
Size: 802 958 1753
Fragments arranged by size:
1753 1340 958 902 802 651 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7381
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 7381
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2775 2915 2963 3026 3137 3191 3515
Size: 992 544 140 48 63 111 54 324
Cuts at: 3515 3536 4016 4088 6041 1239
Size: 21 480 72 1953 2579
Fragments arranged by size:
2579 1953 992 544 480 324 140 111
72 63 54 48 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3290 5506 6371 6669 3290
Size: 2216 865 298 4002
Fragments arranged by size:
4002 2216 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3091 3266 3512 6044 882
Size: 617 1592 175 246 2532 2219
Fragments arranged by size:
2532 2219 1592 617 246 175
BsaAI yAC'GTr
Cuts at: 493 1204 2728 3823 5172 493
Size: 711 1524 1095 1349 2702
Fragments arranged by size:
2702 1524 1349 1095 711
BsaBI GATnn'nnATC
Cuts at: 7375 7375
Size: 7381
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2316 2965 5188 5570
Size: 53 83 186 936 783 649 2223 382
Cuts at: 5570 6205 275
Size: 635 1451
Fragments arranged by size:
2223 1451 936 783 649 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2139 2701 3162 5814 6212 6486 6633 7044 2139
Size: 562 461 2652 398 274 147 411 2476
Fragments arranged by size:
2652 2476 562 461 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2258 2288 1178
Size: 30 165 30 855 30 6271
Fragments arranged by size:
6271 855 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3155 3644 3878 3971 4325 1186
Size: 1969 489 234 93 354 4242
Fragments arranged by size:
4242 1969 489 354 234 93
BseYI C'CCAG_C
Cuts at: 4152 4514 6584 7080 4152
Size: 362 2070 496 4453
Fragments arranged by size:
4453 2070 496 362
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2954 3008 3543 1163
Size: 573 1218 54 535 5001
Fragments arranged by size:
5001 1218 573 535 54
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2702 4831 5592 6620 1540
Size: 275 186 701 2129 761 1028 2301
Fragments arranged by size:
2301 2129 1028 761 701 275 186
BsiHKAI G_wGCw'C
Cuts at: 2268 3387 3621 4140 4633 5442 5527 6598 2268
Size: 1119 234 519 493 809 85 1071 3051
Fragments arranged by size:
3051 1119 1071 809 519 493 234 85
BsmI GAATG_Cn'
Cuts at: 4892 4985 4892
Size: 93 7288
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2268 2761 2910 3387 3469 3621 3770
Size: 399 478 493 149 477 82 152 149
Cuts at: 3770 3916 3936 4140 4633 4700 5442 5527 6598
Size: 146 20 204 493 67 742 85 1071
Cuts at: 6598 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 493 493 478 477 399
204 152 149 149 146 85 82 67
20
BspEI T'CCGG_A
Cuts at: 2139 3162 7044 2139
Size: 1023 3882 2476
Fragments arranged by size:
3882 2476 1023
BspHI T'CATG_A
Cuts at: 2718 4389 5270 7000 2718
Size: 1671 881 1730 3099
Fragments arranged by size:
3099 1730 1671 881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2079 3112 3606 4023 4736 844
Size: 1235 1033 494 417 713 3489
Fragments arranged by size:
3489 1235 1033 713 494 417
BsrBI CCG'CTC
Cuts at: 1394 1823 3499 5268 1394
Size: 429 1676 1769 3507
Fragments arranged by size:
3507 1769 1676 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5876 6050 65
Size: 1183 4628 174 1396
Fragments arranged by size:
4628 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2562 2961 3051 4607 6025 6212 1700
Size: 110 752 399 90 1556 1418 187 2869
Fragments arranged by size:
2869 1556 1418 752 399 187 110 90
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7381
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7381
BssSI C'ACGA_G
Cuts at: 3221 5441 6453 3221
Size: 2220 1012 4149
Fragments arranged by size:
4149 2220 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2929 3835 4379 794
Size: 2135 906 544 3796
Fragments arranged by size:
3796 2135 906 544
BstBI TT'CG_AA
Cuts at: 6185 6185
Size: 7381
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7381
BstXI CCAn_nnnn'nTGG
Cuts at: 2668 4414 2668
Size: 1746 5635
BstYI r'GATC_y
Cuts at: 1972 2271 2871 3159 3438 3633 3939 3969 4206
Size: 299 600 288 279 195 306 30 237
Cuts at: 4206 4506 4711 5078 5463 5480 6932 7018 7030
Size: 300 205 367 385 17 1452 86 12
Cuts at: 7030 7376 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 600 385 367 346 306 300
299 288 279 237 205 195 86 30
17 12
Bsu36I CC'TnA_GG
Cuts at: 3795 3795
Size: 7381
BtgI C'CryG_G
Cuts at: 513 1065 2004 2424 2888 3588 4131 4536 4653
Size: 552 939 420 464 700 543 405 117
Cuts at: 4653 513
Size: 3241
Fragments arranged by size:
3241 939 700 552 543 464 420 405
117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3358 4159 4570 487
Size: 683 56 2132 801 411 3298
Fragments arranged by size:
3298 2132 801 683 411 56
BtsI GCAGTG_nn'
Cuts at: 4897 5691 5711 4897
Size: 794 20 6567
Fragments arranged by size:
6567 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7381
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7346
DraI TTT'AAA
Cuts at: 4685 4685
Size: 7381
DraIII CAC_nnn'GTG
Cuts at: 3416 3416
Size: 7381
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2728 6388 809
Size: 507 1412 3660 1802
Fragments arranged by size:
3660 1802 1412 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2235 2802 2850
Size: 54 1425 50 275 18 405 567 48
Cuts at: 2850 3702 4828 5717 7346 8
Size: 852 1126 889 1629 43
Fragments arranged by size:
1629 1425 1126 889 852 567 405 275
54 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4828 1537
Size: 275 3016 4090
Fragments arranged by size:
4090 3016 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2046 2166 2424 4025 5311 1353
Size: 693 120 258 1601 1286 3423
Fragments arranged by size:
3423 1601 1286 693 258 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2783 2864 5968 6342 6488 387
Size: 1064 138 1194 81 3104 374 146 1280
Fragments arranged by size:
3104 1280 1194 1064 374 146 138 81
EcoICRI GAG'CTC
Cuts at: 3385 3385
Size: 7381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2231 2421 2775 2915 2963 3026
Size: 393 581 18 190 354 140 48 63
Cuts at: 3026 3137 3191 3515 3536 4016 4088 5444 6041
Size: 111 54 324 21 480 72 1356 597
Cuts at: 6041 6828 7096 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1356 787 597 581 480 393 354
324 268 190 140 111 72 63 54
48 21 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2141 3119 2141
Size: 978 6403
EcoO109I rG'GnC_Cy
Cuts at: 2068 2290 3298 3465 3466 3595 3933 3990 4304
Size: 222 1008 167 1 129 338 57 314
Cuts at: 4304 2068
Size: 5145
Fragments arranged by size:
5145 1008 338 314 222 167 129 57
1
EcoRI G'AATT_C
Cuts at: 4690 5068 4690
Size: 378 7003
FspI TGC'GCA
Cuts at: 5887 5887
Size: 7381
HaeII r_GCGC'y
Cuts at: 1678 2968 3700 4100 4661 6528 1678
Size: 1290 732 400 561 1867 2531
Fragments arranged by size:
2531 1867 1290 732 561 400
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4436 4468 4628 4660 6022
Size: 32 135 32 2864 32 160 32 1362
Cuts at: 6022 6054 6096 6128 1373
Size: 32 42 32 2626
Fragments arranged by size:
2864 2626 1362 160 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2284 2497 2809 3421 4042 4725
Size: 988 281 346 213 312 612 621 683
Cuts at: 4725 4971 669
Size: 246 3079
Fragments arranged by size:
3079 988 683 621 612 346 312 281
246 213
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7381
HpaI GTT'AAC
Cuts at: 4971 4971
Size: 7381
KasI G'GCGC_C
Cuts at: 2964 2964
Size: 7381
KpnI G_GTAC'C
Cuts at: 4706 4706
Size: 7381
MfeI C'AATT_G
Cuts at: 4980 4980
Size: 7381
MluI A'CGCG_T
Cuts at: 7257 7257
Size: 7381
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2301 2668 2844 6495 6679 1305
Size: 996 367 176 3651 184 2007
Fragments arranged by size:
3651 2007 996 367 184 176
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2237 2852 3704 10
Size: 54 1425 343 405 615 852 3687
Fragments arranged by size:
3687 1425 852 615 405 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 3980 4143 4145 4535 5339 5698
Size: 648 1257 1557 163 2 390 804 359
Cuts at: 5698 5857 518
Size: 159 2042
Fragments arranged by size:
2042 1557 1257 804 648 390 359 163
159 2
MspA1I CmG'CkG
Cuts at: 1606 3004 3532 3832 4066 4162 4797 5474 6622
Size: 1398 528 300 234 96 635 677 1148
Cuts at: 6622 6867 1606
Size: 245 2120
Fragments arranged by size:
2120 1398 1148 677 635 528 300 245
234 96
NarI GG'CG_CC
Cuts at: 2965 2965
Size: 7381
NcoI C'CATG_G
Cuts at: 513 1065 2004 3588 4131 4536 513
Size: 552 939 1584 543 405 3358
Fragments arranged by size:
3358 1584 939 552 543 405
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7381
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7381
NotI GC'GGCC_GC
Cuts at: 4828 4828
Size: 7381
NsiI A_TGCA'T
Cuts at: 2925 3987 2925
Size: 1062 6319
NspI r_CATG'y
Cuts at: 1132 1229 2044 2927 3872 4645 4739 6284 1132
Size: 97 815 883 945 773 94 1545 2229
Fragments arranged by size:
2229 1545 945 883 815 773 97 94
PasI CC'CwG_GG
Cuts at: 1348 2374 4363 1348
Size: 1026 1989 4366
Fragments arranged by size:
4366 1989 1026
PciI A'CATG_T
Cuts at: 4641 6280 4641
Size: 1639 5742
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4492 1108
Size: 3384 3997
PfoI T'CCnGG_A
Cuts at: 2076 2181 2412 2952 4089 2076
Size: 105 231 540 1137 5368
Fragments arranged by size:
5368 1137 540 231 105
PmeI GTTT'AAAC
Cuts at: 4685 4685
Size: 7381
PmlI CAC'GTG
Cuts at: 3823 3823
Size: 7381
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5450 5482 6963 6995 5450
Size: 32 1481 32 5836
Fragments arranged by size:
5836 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2068 3595 3990 4304 2068
Size: 1527 395 314 5145
Fragments arranged by size:
5145 1527 395 314
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7381
PsiI TTA'TAA
Cuts at: 4951 4951
Size: 7381
PspOMI G'GGCC_C
Cuts at: 3465 3932 3465
Size: 467 6914
PstI C_TGCA'G
Cuts at: 830 3030 3603 4733 830
Size: 2200 573 1130 3478
Fragments arranged by size:
3478 2200 1130 573
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7381
PvuII CAG'CTG
Cuts at: 3004 3532 3832 4066 4162 3004
Size: 528 300 234 96 6223
Fragments arranged by size:
6223 528 300 234 96
RsrII CG'GwC_CG
Cuts at: 3501 3501
Size: 7381
SacI G_AGCT'C
Cuts at: 3387 3387
Size: 7381
SalI G'TCGA_C
Cuts at: 1936 4723 1936
Size: 2787 4594
SanDI GG'GwC_CC
Cuts at: 3990 4304 3990
Size: 314 7067
SapI GCTCTTCn'nnn_
Cuts at: 4025 4025
Size: 7381
SbfI CC_TGCA'GG
Cuts at: 3603 4733 3603
Size: 1130 6251
ScaI AGT'ACT
Cuts at: 4580 5629 7341 4580
Size: 1049 1712 4620
Fragments arranged by size:
4620 1712 1049
SfcI C'TryA_G
Cuts at: 826 1045 3026 3599 4729 5864 6545 6736 826
Size: 219 1981 573 1130 1135 681 191 1471
Fragments arranged by size:
1981 1471 1135 1130 681 573 219 191
SfoI GGC'GCC
Cuts at: 2966 2966
Size: 7381
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7381
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7381
SmaI CCC'GGG
Cuts at: 1872 4217 4708 1872
Size: 2345 491 4545
Fragments arranged by size:
4545 2345 491
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3305 5485 6386 6648 820
Size: 197 926 1362 2180 901 262 1553
Fragments arranged by size:
2180 1553 1362 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5172 493
Size: 4679 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7381
SphI G_CATG'C
Cuts at: 1132 2927 3872 4739 1132
Size: 1795 945 867 3774
Fragments arranged by size:
3774 1795 945 867
SspI AAT'ATT
Cuts at: 5 52 5305 5
Size: 47 5253 2081
Fragments arranged by size:
5253 2081 47
StuI AGG'CCT
Cuts at: 1517 3212 3227 3320 4436 1517
Size: 1695 15 93 1116 4462
Fragments arranged by size:
4462 1695 1116 93 15
StyI C'CwwG_G
Cuts at: 513 1065 2004 2442 2982 3588 4131 4224 4536
Size: 552 939 438 540 606 543 93 312
Cuts at: 4536 4820 513
Size: 284 3074
Fragments arranged by size:
3074 939 606 552 543 540 438 312
284 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3427 3518 5408 5425 5578 5763 6208
Size: 1255 1847 91 1890 17 153 185 445
Cuts at: 6208 325
Size: 1498
Fragments arranged by size:
1890 1847 1498 1255 445 185 153 91
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3534 4578 5627
Size: 274 80 33 51 1443 1557 1044 1049
Cuts at: 5627 7339 96
Size: 1712 138
Fragments arranged by size:
1712 1557 1443 1049 1044 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2258 2290 4288 4320 7341 7373 2258
Size: 32 1998 32 3021 32 2266
Fragments arranged by size:
3021 2266 1998 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4487 1316
Size: 3171 4210
XbaI T'CTAG_A
Cuts at: 4717 4717
Size: 7381
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2055 2750 2860 4129 2055
Size: 695 110 1269 5307
Fragments arranged by size:
5307 1269 695 110
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7381
XmaI C'CCGG_G
Cuts at: 1870 4215 4706 1870
Size: 2345 491 4545
Fragments arranged by size:
4545 2345 491
XmnI GAAnn'nnTTC
Cuts at: 4253 5510 4253
Size: 1257 6124
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5189 276
Size: 53 83 186 936 3655 2468
Fragments arranged by size:
3655 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI
BsmBI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI PacI PspXI PsrI SacII SexAI SfiI SrfI
SwaI