Restriction map for
pFN21AE2246
(Circular) (Six-base) MAPSORT of: pFN21AE2246.seq Check: 9067 from: 1 to: 5605
pFN21AE2246 5605 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3415 278
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2948 1937
Size: 1011 4594
Acc65I G'GTAC_C
Cuts at: 2617 2926 2617
Size: 309 5296
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2789 3668 5052 5320 1632
Size: 1157 879 1384 268 1917
Fragments arranged by size:
1917 1384 1157 879 268
AfeI AGC'GCT
Cuts at: 2264 2888 2264
Size: 624 4981
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 2210 4504 5481 1203
Size: 1007 2294 977 1327
Fragments arranged by size:
2294 1327 1007 977
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 2174 2334 1166
Size: 1008 160 4437
Fragments arranged by size:
4437 1008 160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2192 2332 1184
Size: 1008 140 4457
Fragments arranged by size:
4457 1008 140
AlwNI CAG_nnn'CTG
Cuts at: 2627 2698 2842 3348 4920 2627
Size: 71 144 506 1572 3312
Fragments arranged by size:
3312 1572 506 144 71
ApaLI G'TGCA_C
Cuts at: 2581 2900 3662 4818 2581
Size: 319 762 1156 3368
Fragments arranged by size:
3368 1156 762 319
ApoI r'AATT_y
Cuts at: 1493 2914 3140 3292 5401 1493
Size: 1421 226 152 2109 1697
Fragments arranged by size:
2109 1697 1421 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2425 2711 2861 2930 1870
Size: 73 482 286 150 69 4545
Fragments arranged by size:
4545 482 286 150 73 69
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2601 2634 2601
Size: 33 5572
BamHI G'GATC_C
Cuts at: 2935 3302 2935
Size: 367 5238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2074 2168 2408 2429 2471
Size: 325 217 627 287 94 240 21 42
Cuts at: 2471 2617 2926 4381 618
Size: 146 309 1455 1842
Fragments arranged by size:
1842 1455 627 325 309 287 240 217
146 94 42 21
BanII G_rGCy'C
Cuts at: 1391 2796 2924 1391
Size: 1405 128 4072
Fragments arranged by size:
4072 1405 128
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4634
BbvCI CC'TCA_GC
Cuts at: 2606 2628 2606
Size: 22 5583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3796 3830 1903
Size: 34 1859 34 3678
Fragments arranged by size:
3678 1859 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3497 4707 3497
Size: 1210 4395
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2166 2355
Size: 107 122 71 631 605 337 157 189
Cuts at: 2355 2469 2714 4216 136
Size: 114 245 1502 1525
Fragments arranged by size:
1525 1502 631 605 337 245 189 157
122 114 107 71
BglII A'GATC_T
Cuts at: 5600 5600
Size: 5605
BlpI GC'TnA_GC
Cuts at: 3022 3022
Size: 5605
Bme1580I G_kGCm'C
Cuts at: 1790 2162 2432 2465 2585 2904 3666 4822 1790
Size: 372 270 33 120 319 762 1156 2573
Fragments arranged by size:
2573 1156 762 372 319 270 120 33
BmgBI CAC'GTC
Cuts at: 1318 2211 1318
Size: 893 4712
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2149 3336 4294
Size: 416 53 83 399 24 732 1187 958
Cuts at: 4294 442
Size: 1753
Fragments arranged by size:
1753 1187 958 732 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2279 2476 2888 4265 1239
Size: 797 243 197 412 1377 2579
Fragments arranged by size:
2579 1377 797 412 243 197
Bpu10I CC'TnA_GC
Cuts at: 2606 2628 2606
Size: 22 5583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3730 4595 4893 3730
Size: 865 298 4442
Fragments arranged by size:
4442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4268 882
Size: 617 2769 2219
Fragments arranged by size:
2769 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3396 493
Size: 711 2192 2702
Fragments arranged by size:
2702 2192 711
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3412 3794 4429 275
Size: 53 83 186 936 1879 382 635 1451
Fragments arranged by size:
1879 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4038 4436 4710 4857 5268 4038
Size: 398 274 147 411 4375
Fragments arranged by size:
4375 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2866 2896 1178
Size: 30 165 30 1463 30 3887
Fragments arranged by size:
3887 1463 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2315 2318 2645 1186
Size: 1129 3 327 4146
Fragments arranged by size:
4146 1129 327 3
BseYI C'CCAG_C
Cuts at: 2435 2727 4808 5304 2435
Size: 292 2081 496 2736
Fragments arranged by size:
2736 2081 496 292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 2509 2575 1163
Size: 573 462 311 66 4193
Fragments arranged by size:
4193 573 462 311 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2186 3055 3816 4844 1540
Size: 275 186 185 869 761 1028 2301
Fragments arranged by size:
2301 1028 869 761 275 186 185
BsiHKAI G_wGCw'C
Cuts at: 2391 2585 2796 2904 3666 3751 4822 2391
Size: 194 211 108 762 85 1071 3174
Fragments arranged by size:
3174 1071 762 211 194 108 85
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 2391 2432 2465 2585 2796 2904
Size: 399 372 229 41 33 120 211 108
Cuts at: 2904 2924 3666 3751 4822 1391
Size: 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 372 229 211 120
108 85 41 33 20
BspEI T'CCGG_A
Cuts at: 5268 5268
Size: 5605
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2960 844
Size: 2116 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2140 2872 3492 1394
Size: 429 317 732 620 3507
Fragments arranged by size:
3507 732 620 429 317
BsrDI GCAATG_nn'
Cuts at: 65 1248 4100 4274 65
Size: 1183 2852 174 1396
Fragments arranged by size:
2852 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2071 2077 2354 2411 4249 4436 1700
Size: 110 261 6 277 57 1838 187 2869
Fragments arranged by size:
2869 1838 277 261 187 110 57 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2589 2698 2842 794
Size: 1795 109 144 3557
Fragments arranged by size:
3557 1795 144 109
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstYI r'GATC_y
Cuts at: 1972 2935 3302 3687 3704 5156 5242 5254 5600
Size: 963 367 385 17 1452 86 12 346
Cuts at: 5600 1972
Size: 1977
Fragments arranged by size:
1977 1452 963 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5053
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4866
Fragments arranged by size:
4866 683 56
BtsI GCAGTG_nn'
Cuts at: 2456 2510 3121 3915 3935 2456
Size: 54 611 794 20 4126
Fragments arranged by size:
4126 794 611 54 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5570
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DraIII CAC_nnn'GTG
Cuts at: 2211 2408 2451 2580 2211
Size: 197 43 129 5236
Fragments arranged by size:
5236 197 129 43
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4612 809
Size: 507 3296 1802
Fragments arranged by size:
3296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2183 2850 3052
Size: 54 1425 50 275 18 353 667 202
Cuts at: 3052 3941 5570 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 667 353 275 202 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2183 3052 1537
Size: 275 371 869 4090
Fragments arranged by size:
4090 869 371 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2566 3535 1353
Size: 1213 969 3423
Fragments arranged by size:
3423 1213 969
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4192 4566 4712 387
Size: 1064 138 2603 374 146 1280
Fragments arranged by size:
2603 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2794 2794
Size: 5605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2279 2476 2789 2888 3668 4265
Size: 393 404 243 197 313 99 780 597
Cuts at: 4265 5052 5320 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 780 597 404 393 313 268
243 197 99
EcoO109I rG'GnC_Cy
Cuts at: 2707 2716 2707
Size: 9 5596
EcoRI G'AATT_C
Cuts at: 2914 3292 2914
Size: 378 5227
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 2015 2057 2266 2819 2843 2890 4752 1678
Size: 337 42 209 553 24 47 1862 2531
Fragments arranged by size:
2531 1862 553 337 209 47 42 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2378 2410 4246 4278 4320
Size: 32 135 32 806 32 1836 32 42
Cuts at: 4320 4352 1373
Size: 32 2626
Fragments arranged by size:
2626 1836 806 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2949 3195 669
Size: 988 281 1011 246 3079
Fragments arranged by size:
3079 1011 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5605
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KpnI G_GTAC'C
Cuts at: 2621 2930 2621
Size: 309 5296
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 5481 5481
Size: 5605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2759 4719 4903 1305
Size: 1454 1960 184 2007
Fragments arranged by size:
2007 1960 1454 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2852 10
Size: 54 1425 343 1020 2763
Fragments arranged by size:
2763 1425 1020 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 2334 3563 3922 4081 518
Size: 648 1008 160 1229 359 159 2042
Fragments arranged by size:
2042 1229 1008 648 359 160 159
MspA1I CmG'CkG
Cuts at: 1606 2182 2346 2589 2695 2767 3021 3698 4846
Size: 576 164 243 106 72 254 677 1148
Cuts at: 4846 5091 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 576 254 245 243 164
106 72
NaeI GCC'GGC
Cuts at: 2079 2413 2079
Size: 334 5271
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5053
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NgoMIV G'CCGG_C
Cuts at: 2077 2411 2077
Size: 334 5271
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2963 4508 1132
Size: 97 1734 1545 2229
Fragments arranged by size:
2229 1734 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2533 1348
Size: 1185 4420
PciI A'CATG_T
Cuts at: 4504 4504
Size: 5605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5605
PfoI T'CCnGG_A
Cuts at: 2667 2667
Size: 5605
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 5187 5219 3674
Size: 32 1481 32 4060
Fragments arranged by size:
4060 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PspXI vC'TCGA_Gb
Cuts at: 2711 2711
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2694 2706 2838 2957 830
Size: 1864 12 132 119 3478
Fragments arranged by size:
3478 1864 132 119 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
PvuII CAG'CTG
Cuts at: 2589 2767 2589
Size: 178 5427
SacI G_AGCT'C
Cuts at: 2796 2796
Size: 5605
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SapI GCTCTTCn'nnn_
Cuts at: 2566 2566
Size: 5605
SbfI CC_TGCA'GG
Cuts at: 2957 2957
Size: 5605
ScaI AGT'ACT
Cuts at: 3853 5565 3853
Size: 1712 3893
SexAI A'CCwGG_T
Cuts at: 2404 2404
Size: 5605
SfcI C'TryA_G
Cuts at: 826 1045 2690 2702 2834 2953 4088 4769 4960
Size: 219 1645 12 132 119 1135 681 191
Cuts at: 4960 826
Size: 1471
Fragments arranged by size:
1645 1471 1135 681 219 191 132 119
12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2714 2714
Size: 5605
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5605
SmaI CCC'GGG
Cuts at: 1872 2427 2863 2932 1872
Size: 555 436 69 4545
Fragments arranged by size:
4545 555 436 69
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2711 3709 4610 4872 820
Size: 197 926 768 998 901 262 1553
Fragments arranged by size:
1553 998 926 901 768 262 197
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SrfI GCCC'GGGC
Cuts at: 2863 2863
Size: 5605
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 2028 1517
Size: 511 5094
StyI C'CwwG_G
Cuts at: 513 1065 2741 3044 513
Size: 552 1676 303 3074
Fragments arranged by size:
3074 1676 552 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2119 2342 3632 3649 3802 3987 4432
Size: 1255 539 223 1290 17 153 185 445
Cuts at: 4432 325
Size: 1498
Fragments arranged by size:
1498 1290 1255 539 445 223 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3851 5563 96
Size: 274 80 33 51 1443 1874 1712 138
Fragments arranged by size:
1874 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2866 2898 5565 5597 2866
Size: 32 2667 32 2874
Fragments arranged by size:
2874 2667 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5605
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 2711 1943
Size: 768 4837
XmaI C'CCGG_G
Cuts at: 1870 2425 2861 2930 1870
Size: 555 436 69 4545
Fragments arranged by size:
4545 555 436 69
XmnI GAAnn'nnTTC
Cuts at: 3734 3734
Size: 5605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3413 276
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PpuMI PspOMI PsrI RsrII SacII SanDI SfoI SwaI