Restriction map for
pFN21AE2241
(Circular) (Six-base) MAPSORT of: pFN21AE2241.seq Check: 595 from: 1 to: 6232
pFN21AE2241 6232 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2248 3011 2248
Size: 763 5469
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2220 4042 278
Size: 53 83 186 936 684 1822 2468
Fragments arranged by size:
2468 1822 936 684 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2205 3575 1937
Size: 268 1370 4594
Fragments arranged by size:
4594 1370 268
Acc65I G'GTAC_C
Cuts at: 3553 3553
Size: 6232
AclI AA'CG_TT
Cuts at: 1563 4359 4732 1563
Size: 2796 373 3063
Fragments arranged by size:
3063 2796 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2261 2489 4295 5679 5947 1632
Size: 629 228 1806 1384 268 1917
Fragments arranged by size:
1917 1806 1384 629 268 228
AflII C'TTAA_G
Cuts at: 820 1017 2126 820
Size: 197 1109 4926
Fragments arranged by size:
4926 1109 197
AflIII A'CryG_T
Cuts at: 1203 2166 2280 5131 6108 1203
Size: 963 114 2851 977 1327
Fragments arranged by size:
2851 1327 977 963 114
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 2335 4961 2335
Size: 2626 3606
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6232
AlwNI CAG_nnn'CTG
Cuts at: 3003 3975 5547 3003
Size: 972 1572 3688
Fragments arranged by size:
3688 1572 972
ApaI G_GGCC'C
Cuts at: 2277 2277
Size: 6232
ApaLI G'TGCA_C
Cuts at: 4289 5445 4289
Size: 1156 5076
ApoI r'AATT_y
Cuts at: 1493 2985 3541 3767 3919 6028 1493
Size: 1492 556 226 152 2109 1697
Fragments arranged by size:
2109 1697 1492 556 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4786 160
Size: 872 3754 1606
Fragments arranged by size:
3754 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 2345 3557 1870
Size: 73 402 1212 4545
Fragments arranged by size:
4545 1212 402 73
BamHI G'GATC_C
Cuts at: 2694 3562 3929 2694
Size: 868 367 4997
Fragments arranged by size:
4997 868 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2098 3553 5008 618
Size: 325 217 627 311 1455 1455 1842
Fragments arranged by size:
1842 1455 1455 627 325 311 217
BanII G_rGCy'C
Cuts at: 1391 2277 2637 2658 3446 3509 3551 1391
Size: 886 360 21 788 63 42 4072
Fragments arranged by size:
4072 886 788 360 63 42 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5261
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4423 4457 1903
Size: 34 2486 34 3678
Fragments arranged by size:
3678 2486 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4124 5334 4124
Size: 1210 5022
BclI T'GATC_A
Cuts at: 1548 2763 1548
Size: 1215 5017
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3467 4843 136
Size: 107 122 71 631 605 1795 1376 1525
Fragments arranged by size:
1795 1525 1376 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6227 6227
Size: 6232
BlpI GC'TnA_GC
Cuts at: 2631 2723 3173 3649 2631
Size: 92 450 476 5214
Fragments arranged by size:
5214 476 450 92
Bme1580I G_kGCm'C
Cuts at: 1790 2277 3019 4293 5449 1790
Size: 487 742 1274 1156 2573
Fragments arranged by size:
2573 1274 1156 742 487
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6232
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2142 3222 3331
Size: 416 53 83 399 24 725 1080 109
Cuts at: 3331 3963 4921 442
Size: 632 958 1753
Fragments arranged by size:
1753 1080 958 725 632 416 399 109
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6232
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3124 3124
Size: 6232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2840 2996 4892 1239
Size: 1601 156 1896 2579
Fragments arranged by size:
2579 1896 1601 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4357 5222 5520 4357
Size: 865 298 5069
Fragments arranged by size:
5069 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4895 882
Size: 617 3396 2219
Fragments arranged by size:
3396 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2281 4023 493
Size: 711 1077 1742 2702
Fragments arranged by size:
2702 1742 1077 711
BsaBI GATnn'nnATC
Cuts at: 2183 6226 2183
Size: 4043 2189
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2217 4039 4421
Size: 53 83 186 936 516 168 1822 382
Cuts at: 4421 5056 275
Size: 635 1451
Fragments arranged by size:
1822 1451 936 635 516 382 186 168
83 53
BsaWI w'CCGG_w
Cuts at: 3396 4665 5063 5337 5484 5895 3396
Size: 1269 398 274 147 411 3733
Fragments arranged by size:
3733 1269 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3418 3448 3481 3511 1178
Size: 30 165 30 2015 30 33 30 3899
Fragments arranged by size:
3899 2015 165 33 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6232
BseYI C'CCAG_C
Cuts at: 2028 2044 2074 2228 3355 5435 5931 2028
Size: 16 30 154 1127 2080 496 2329
Fragments arranged by size:
2329 2080 1127 496 154 30 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3071 1163
Size: 573 1335 4324
Fragments arranged by size:
4324 1335 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3682 4443 5471 1540
Size: 275 186 1681 761 1028 2301
Fragments arranged by size:
2301 1681 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2637 4293 4378 5449 2637
Size: 1656 85 1071 3420
Fragments arranged by size:
3420 1656 1071 85
BsmI GAATG_Cn'
Cuts at: 2489 2743 3743 3836 2489
Size: 254 1000 93 4885
Fragments arranged by size:
4885 1000 254 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2277 2637 2658 3019 3446 3509 3551
Size: 399 487 360 21 361 427 63 42
Cuts at: 3551 4293 4378 5449 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 487 427 399 361 360
85 63 42 21
BspEI T'CCGG_A
Cuts at: 3396 5895 3396
Size: 2499 3733
BspHI T'CATG_A
Cuts at: 2085 2220 2478 2517 2799 4121 5851 2085
Size: 135 258 39 282 1322 1730 2466
Fragments arranged by size:
2466 1730 1322 282 258 135 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2110 2248 2997 3011 3587 844
Size: 1266 138 749 14 576 3489
Fragments arranged by size:
3489 1266 749 576 138 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3261 4119 1394
Size: 429 1438 858 3507
Fragments arranged by size:
3507 1438 858 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4727 4901 65
Size: 1183 3479 174 1396
Fragments arranged by size:
3479 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4876 5063 1700
Size: 110 3066 187 2869
Fragments arranged by size:
3066 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2283 2862 96
Size: 2187 579 3466
Fragments arranged by size:
3466 2187 579
BssHII G'CGCG_C
Cuts at: 1925 2024 1925
Size: 99 6133
BssSI C'ACGA_G
Cuts at: 2702 4292 5304 2702
Size: 1590 1012 3630
Fragments arranged by size:
3630 1590 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6232
BstBI TT'CG_AA
Cuts at: 5036 5036
Size: 6232
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6232
BstXI CCAn_nnnn'nTGG
Cuts at: 2422 2422
Size: 6232
BstYI r'GATC_y
Cuts at: 1972 2178 2235 2694 3562 3929 4314 4331 5783
Size: 206 57 459 868 367 385 17 1452
Cuts at: 5783 5869 5881 6227 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 868 459 385 367 346 206
86 57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2383 3267 513
Size: 552 939 379 884 3478
Fragments arranged by size:
3478 939 884 552 379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5493
Fragments arranged by size:
5493 683 56
BtsI GCAGTG_nn'
Cuts at: 3748 4542 4562 3748
Size: 794 20 5418
Fragments arranged by size:
5418 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6197
DraI TTT'AAA
Cuts at: 3536 3536
Size: 6232
DraIII CAC_nnn'GTG
Cuts at: 2704 2704
Size: 6232
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2405 2809 5239 809
Size: 507 1089 404 2430 1802
Fragments arranged by size:
2430 1802 1089 507 404
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2789 3679 4568
Size: 54 1425 50 275 18 959 890 889
Cuts at: 4568 6197 8
Size: 1629 43
Fragments arranged by size:
1629 1425 959 890 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3679 1537
Size: 275 1867 4090
Fragments arranged by size:
4090 1867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2663 3288 4162 1353
Size: 1310 625 874 3423
Fragments arranged by size:
3423 1310 874 625
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2005 4819 5193 5339 387
Size: 1064 138 416 2814 374 146 1280
Fragments arranged by size:
2814 1280 1064 416 374 146 138
EcoICRI GAG'CTC
Cuts at: 2635 2635
Size: 6232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2489 2840 2996 4295 4892 5679
Size: 393 629 228 351 156 1299 597 787
Cuts at: 5679 5947 1239
Size: 268 1524
Fragments arranged by size:
1524 1299 787 629 597 393 351 268
228 156
EcoO109I rG'GnC_Cy
Cuts at: 2122 2274 2557 2122
Size: 152 283 5797
Fragments arranged by size:
5797 283 152
EcoRI G'AATT_C
Cuts at: 2985 3541 3919 2985
Size: 556 378 5298
Fragments arranged by size:
5298 556 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 6232
FspI TGC'GCA
Cuts at: 2138 4738 2138
Size: 2600 3632
FspAI rTGC'GCAy
Cuts at: 2138 2138
Size: 6232
HaeII r_GCGC'y
Cuts at: 1678 2045 2082 3356 5379 1678
Size: 367 37 1274 2023 2531
Fragments arranged by size:
2531 2023 1274 367 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 3481 3513 4873
Size: 32 135 32 749 32 1128 32 1360
Cuts at: 4873 4905 4947 4979 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1360 1128 749 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2734 3576 3822 669
Size: 988 281 796 842 246 3079
Fragments arranged by size:
3079 988 842 796 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6232
HpaI GTT'AAC
Cuts at: 3822 3822
Size: 6232
KpnI G_GTAC'C
Cuts at: 3557 3557
Size: 6232
MfeI C'AATT_G
Cuts at: 3831 3831
Size: 6232
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2670 5346 5530 1305
Size: 1365 2676 184 2007
Fragments arranged by size:
2676 2007 1365 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4410
Fragments arranged by size:
4410 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 2408 4190 4549 4708 518
Size: 648 924 318 1782 359 159 2042
Fragments arranged by size:
2042 1782 924 648 359 318 159
MspA1I CmG'CkG
Cuts at: 1606 3172 3241 3304 3648 4325 5473 5718 1606
Size: 1566 69 63 344 677 1148 245 2120
Fragments arranged by size:
2120 1566 1148 677 344 245 69 63
NcoI C'CATG_G
Cuts at: 513 1065 2004 3267 513
Size: 552 939 1263 3478
Fragments arranged by size:
3478 1263 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6232
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6232
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NspI r_CATG'y
Cuts at: 1132 1229 2170 2599 3590 5135 1132
Size: 97 941 429 991 1545 2229
Fragments arranged by size:
2229 1545 991 941 429 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6232
PciI A'CATG_T
Cuts at: 2166 5131 2166
Size: 2965 3267
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2704 1108
Size: 1596 4636
PfoI T'CCnGG_A
Cuts at: 3117 3117
Size: 6232
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 5814 5846 4301
Size: 32 1481 32 4687
Fragments arranged by size:
4687 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2122 2557 2122
Size: 435 5797
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6232
PsiI TTA'TAA
Cuts at: 3802 3802
Size: 6232
PspOMI G'GGCC_C
Cuts at: 2273 2273
Size: 6232
PspXI vC'TCGA_Gb
Cuts at: 2345 2345
Size: 6232
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2276 2308 2276
Size: 32 6200
PstI C_TGCA'G
Cuts at: 830 3008 3171 3519 3584 830
Size: 2178 163 348 65 3478
Fragments arranged by size:
3478 2178 348 163 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6232
PvuII CAG'CTG
Cuts at: 3172 3304 3172
Size: 132 6100
SacI G_AGCT'C
Cuts at: 2637 2637
Size: 6232
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SapI GCTCTTCn'nnn_
Cuts at: 2663 2663
Size: 6232
SbfI CC_TGCA'GG
Cuts at: 3008 3584 3008
Size: 576 5656
ScaI AGT'ACT
Cuts at: 2585 4480 6192 2585
Size: 1895 1712 2625
Fragments arranged by size:
2625 1895 1712
SfcI C'TryA_G
Cuts at: 826 1045 2827 3004 3167 3515 3580 4715 5396
Size: 219 1782 177 163 348 65 1135 681
Cuts at: 5396 5587 826
Size: 191 1471
Fragments arranged by size:
1782 1471 1135 681 348 219 191 177
163 65
SfiI GGCCn_nnn'nGGCC
Cuts at: 3467 3467
Size: 6232
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 3559 1872
Size: 1687 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2126 2345 4336 5237 5499 820
Size: 197 926 183 219 1991 901 262 1553
Fragments arranged by size:
1991 1553 926 901 262 219 197 183
SnaBI TAC'GTA
Cuts at: 493 4023 493
Size: 3530 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6232
SphI G_CATG'C
Cuts at: 1132 3590 1132
Size: 2458 3774
SspI AAT'ATT
Cuts at: 5 52 4156 5
Size: 47 4104 2081
Fragments arranged by size:
4104 2081 47
StuI AGG'CCT
Cuts at: 1517 2840 3470 1517
Size: 1323 630 4279
Fragments arranged by size:
4279 1323 630
StyI C'CwwG_G
Cuts at: 513 1065 2004 2484 3267 3318 3501 3671 513
Size: 552 939 480 783 51 183 170 3074
Fragments arranged by size:
3074 939 783 552 480 183 170 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2420 2522 2965 4259 4276 4429 4614
Size: 1255 840 102 443 1294 17 153 185
Cuts at: 4614 5059 325
Size: 445 1498
Fragments arranged by size:
1498 1294 1255 840 445 443 185 153
102 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2283 2583 2862
Size: 274 80 33 51 1443 306 300 279
Cuts at: 2862 4478 6190 96
Size: 1616 1712 138
Fragments arranged by size:
1712 1616 1443 306 300 279 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6192 6224 6192
Size: 32 6200
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6232
XbaI T'CTAG_A
Cuts at: 3568 3568
Size: 6232
XhoI C'TCGA_G
Cuts at: 1943 2345 1943
Size: 402 5830
XmaI C'CCGG_G
Cuts at: 1870 3557 1870
Size: 1687 4545
XmnI GAAnn'nnTTC
Cuts at: 4361 4361
Size: 6232
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2218 4040 276
Size: 53 83 186 936 684 1822 2468
Fragments arranged by size:
2468 1822 936 684 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
RsrII SacII SanDI SexAI SfoI SrfI SwaI XcmI