Restriction map for
pFN21AE2239
(Circular) (Six-base) MAPSORT of: pFN21AE2239.seq Check: 5132 from: 1 to: 6004
pFN21AE2239 6004 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3347 1937
Size: 1410 4594
Acc65I G'GTAC_C
Cuts at: 2260 3325 2260
Size: 1065 4939
AclI AA'CG_TT
Cuts at: 1563 4131 4504 1563
Size: 2568 373 3063
Fragments arranged by size:
3063 2568 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2193 3141 4067 5451 5719 1632
Size: 561 948 926 1384 268 1917
Fragments arranged by size:
1917 1384 948 926 561 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 4903 5880 1203
Size: 3700 977 1327
Fragments arranged by size:
3700 1327 977
AgeI A'CCGG_T
Cuts at: 4835 4835
Size: 6004
AhdI GACnn_n'nnGTC
Cuts at: 4733 4733
Size: 6004
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6004
AlwNI CAG_nnn'CTG
Cuts at: 2216 2288 2312 2324 3151 3747 5319 2216
Size: 72 24 12 827 596 1572 2901
Fragments arranged by size:
2901 1572 827 596 72 24 12
ApaLI G'TGCA_C
Cuts at: 3014 4061 5217 3014
Size: 1047 1156 3801
Fragments arranged by size:
3801 1156 1047
ApoI r'AATT_y
Cuts at: 1493 2648 3066 3259 3313 3539 3691 5800 1493
Size: 1155 418 193 54 226 152 2109 1697
Fragments arranged by size:
2109 1697 1155 418 226 193 152 54
AseI AT'TA_AT
Cuts at: 160 1032 2037 4558 160
Size: 872 1005 2521 1606
Fragments arranged by size:
2521 1606 1005 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 3329 1870
Size: 73 1386 4545
Fragments arranged by size:
4545 1386 73
BamHI G'GATC_C
Cuts at: 3334 3701 3334
Size: 367 5637
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2260 2272 3325 4780 618
Size: 325 217 627 473 12 1053 1455 1842
Fragments arranged by size:
1842 1455 1053 627 473 325 217 12
BanII G_rGCy'C
Cuts at: 1391 3323 1391
Size: 1932 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2430 928
Size: 971 531 4502
Fragments arranged by size:
4502 971 531
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2903 2937 4195 4229 1903
Size: 34 966 34 1258 34 3678
Fragments arranged by size:
3678 1258 966 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6004
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4615 136
Size: 107 122 71 631 605 2943 1525
Fragments arranged by size:
2943 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5999 5999
Size: 6004
BlpI GC'TnA_GC
Cuts at: 3055 3421 3055
Size: 366 5638
Bme1580I G_kGCm'C
Cuts at: 1790 2413 2724 3018 4065 5221 1790
Size: 623 311 294 1047 1156 2573
Fragments arranged by size:
2573 1156 1047 623 311 294
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6004
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2591 3735 4693
Size: 416 53 83 399 24 1174 1144 958
Cuts at: 4693 442
Size: 1753
Fragments arranged by size:
1753 1174 1144 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2778 4664 1239
Size: 1539 1886 2579
Fragments arranged by size:
2579 1886 1539
Bpu10I CC'TnA_GC
Cuts at: 2313 2325 2355 2313
Size: 12 30 5962
Fragments arranged by size:
5962 30 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2567 4129 4994 5292 2567
Size: 1562 865 298 3279
Fragments arranged by size:
3279 1562 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2660 4667 882
Size: 617 1161 2007 2219
Fragments arranged by size:
2219 2007 1161 617
BsaAI yAC'GTr
Cuts at: 493 1204 3795 493
Size: 711 2591 2702
Fragments arranged by size:
2702 2591 711
BsaBI GATnn'nnATC
Cuts at: 5998 5998
Size: 6004
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3811 4193 4828 275
Size: 53 83 186 936 2278 382 635 1451
Fragments arranged by size:
2278 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4437 4835 5109 5256 5667 4437
Size: 398 274 147 411 4774
Fragments arranged by size:
4774 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2365 2395 2657 2687 1178
Size: 30 165 30 962 30 262 30 4495
Fragments arranged by size:
4495 962 262 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6004
BseYI C'CCAG_C
Cuts at: 5207 5703 5207
Size: 496 5508
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3249 1163
Size: 573 1513 3918
Fragments arranged by size:
3918 1513 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3454 4215 5243 1540
Size: 275 186 1453 761 1028 2301
Fragments arranged by size:
2301 1453 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3018 4065 4150 5221 3018
Size: 1047 85 1071 3801
Fragments arranged by size:
3801 1071 1047 85
BsmI GAATG_Cn'
Cuts at: 3515 3608 3515
Size: 93 5911
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2413 2724 3018 3323 4065 4150 5221
Size: 399 623 311 294 305 742 85 1071
Cuts at: 5221 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 623 399 311 305 294
85
BspEI T'CCGG_A
Cuts at: 5667 5667
Size: 6004
BspHI T'CATG_A
Cuts at: 3893 5623 3893
Size: 1730 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3359 844
Size: 2515 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3891 1394
Size: 429 2068 3507
Fragments arranged by size:
3507 2068 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4499 4673 65
Size: 1183 3251 174 1396
Fragments arranged by size:
3251 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4648 4835 1700
Size: 110 2838 187 2869
Fragments arranged by size:
2869 2838 187 110
BsrGI T'GTAC_A
Cuts at: 96 2673 96
Size: 2577 3427
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6004
BssSI C'ACGA_G
Cuts at: 4064 5076 4064
Size: 1012 4992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6004
BstBI TT'CG_AA
Cuts at: 4808 4808
Size: 6004
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6004
BstXI CCAn_nnnn'nTGG
Cuts at: 3292 3292
Size: 6004
BstYI r'GATC_y
Cuts at: 1972 3334 3701 4086 4103 5555 5641 5653 5999
Size: 1362 367 385 17 1452 86 12 346
Cuts at: 5999 1972
Size: 1977
Fragments arranged by size:
1977 1452 1362 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4513
Fragments arranged by size:
4513 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5265
Fragments arranged by size:
5265 683 56
BtsI GCAGTG_nn'
Cuts at: 2751 3520 4314 4334 2751
Size: 769 794 20 4421
Fragments arranged by size:
4421 794 769 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5969
DraI TTT'AAA
Cuts at: 2883 3308 2883
Size: 425 5579
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5011 809
Size: 507 3695 1802
Fragments arranged by size:
3695 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2970 3451 4340
Size: 54 1425 50 275 18 1140 481 889
Cuts at: 4340 5969 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1140 889 481 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3451 1537
Size: 275 1639 4090
Fragments arranged by size:
4090 1639 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2547 3135 3934 1353
Size: 1194 588 799 3423
Fragments arranged by size:
3423 1194 799 588
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4591 4965 5111 387
Size: 1064 138 3002 374 146 1280
Fragments arranged by size:
3002 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2193 2778 3141 4067 4664 5451 5719
Size: 393 561 585 363 926 597 787 268
Cuts at: 5719 1239
Size: 1524
Fragments arranged by size:
1524 926 787 597 585 561 393 363
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2432 2432
Size: 6004
EcoRI G'AATT_C
Cuts at: 3259 3313 3691 3259
Size: 54 378 5572
Fragments arranged by size:
5572 378 54
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2363 3146 2363
Size: 783 5221
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 2012 5151 1678
Size: 334 3139 2531
Fragments arranged by size:
3139 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2395 3079 3111 3229
Size: 32 135 32 791 32 684 32 118
Cuts at: 3229 3261 4645 4677 4719 4751 1373
Size: 32 1384 32 42 32 2626
Fragments arranged by size:
2626 1384 791 684 135 118 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3348 3594 669
Size: 988 281 1410 246 3079
Fragments arranged by size:
3079 1410 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6004
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KpnI G_GTAC'C
Cuts at: 2264 3329 2264
Size: 1065 4939
MfeI C'AATT_G
Cuts at: 3603 3603
Size: 6004
MluI A'CGCG_T
Cuts at: 5880 5880
Size: 6004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2979 5118 5302 1305
Size: 1674 2139 184 2007
Fragments arranged by size:
2139 2007 1674 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2972 10
Size: 54 1425 343 1140 3042
Fragments arranged by size:
3042 1425 1140 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2404 2675 2756 3962 4321 4480 518
Size: 648 1238 271 81 1206 359 159 2042
Fragments arranged by size:
2042 1238 1206 648 359 271 159 81
MspA1I CmG'CkG
Cuts at: 1606 2740 3256 3420 4097 5245 5490 1606
Size: 1134 516 164 677 1148 245 2120
Fragments arranged by size:
2120 1148 1134 677 516 245 164
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4513
Fragments arranged by size:
4513 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 3233 3362 4907 1132
Size: 97 2004 129 1545 2229
Fragments arranged by size:
2229 2004 1545 129 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6004
PciI A'CATG_T
Cuts at: 4903 4903
Size: 6004
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2801 1108
Size: 1693 4311
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 5586 5618 4073
Size: 32 1481 32 4459
Fragments arranged by size:
4459 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2430 1613
Size: 817 5187
PsiI TTA'TAA
Cuts at: 3574 3574
Size: 6004
PstI C_TGCA'G
Cuts at: 830 2175 2419 2739 3356 830
Size: 1345 244 320 617 3478
Fragments arranged by size:
3478 1345 617 320 244
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
PvuII CAG'CTG
Cuts at: 2740 3256 2740
Size: 516 5488
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SapI GCTCTTCn'nnn_
Cuts at: 2547 2547
Size: 6004
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 4252 5964 4252
Size: 1712 4292
SfcI C'TryA_G
Cuts at: 826 1045 2171 2415 2735 3352 4487 5168 5359
Size: 219 1126 244 320 617 1135 681 191
Cuts at: 5359 826
Size: 1471
Fragments arranged by size:
1471 1135 1126 681 617 320 244 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 3331 1872
Size: 1459 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2546 4108 5009 5271 820
Size: 197 926 603 1562 901 262 1553
Fragments arranged by size:
1562 1553 926 901 603 262 197
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 3268 152
Size: 3116 2888
SphI G_CATG'C
Cuts at: 1132 3233 3362 1132
Size: 2101 129 3774
Fragments arranged by size:
3774 2101 129
SspI AAT'ATT
Cuts at: 5 52 3928 5
Size: 47 3876 2081
Fragments arranged by size:
3876 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6004
StyI C'CwwG_G
Cuts at: 513 1065 2004 3285 3443 513
Size: 552 939 1281 158 3074
Fragments arranged by size:
3074 1281 939 552 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4031 4048 4201 4386 4831 325
Size: 1255 2451 17 153 185 445 1498
Fragments arranged by size:
2451 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2632 2673 4250
Size: 274 80 33 51 1443 655 41 1577
Cuts at: 4250 5962 96
Size: 1712 138
Fragments arranged by size:
1712 1577 1443 655 274 138 80 51
41 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5964 5996 5964
Size: 32 5972
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6004
XbaI T'CTAG_A
Cuts at: 3340 3340
Size: 6004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2980 2980
Size: 6004
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6004
XmaI C'CCGG_G
Cuts at: 1870 3329 1870
Size: 1459 4545
XmnI GAAnn'nnTTC
Cuts at: 3129 4133 3129
Size: 1004 5000
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI