Restriction map for
pFN21AE2234
(Circular) (Six-base) MAPSORT of: pFN21AE2234.seq Check: 3668 from: 1 to: 5494
pFN21AE2234 5494 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2048 2048
Size: 5494
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3304 278
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2837 1937
Size: 900 4594
Acc65I G'GTAC_C
Cuts at: 2815 2815
Size: 5494
AclI AA'CG_TT
Cuts at: 1563 3621 3994 1563
Size: 2058 373 3063
Fragments arranged by size:
3063 2058 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2270 2675 3557 4941 5209 1632
Size: 638 405 882 1384 268 1917
Fragments arranged by size:
1917 1384 882 638 405 268
AfeI AGC'GCT
Cuts at: 2270 2270
Size: 5494
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5297
AflIII A'CryG_T
Cuts at: 1203 4393 5370 1203
Size: 3190 977 1327
Fragments arranged by size:
3190 1327 977
AgeI A'CCGG_T
Cuts at: 4325 4325
Size: 5494
AhdI GACnn_n'nnGTC
Cuts at: 4223 4223
Size: 5494
AleI CACnn'nnGTG
Cuts at: 1166 2484 2579 1166
Size: 1318 95 4081
Fragments arranged by size:
4081 1318 95
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2514 1184
Size: 1330 4164
AlwNI CAG_nnn'CTG
Cuts at: 2653 3237 4809 2653
Size: 584 1572 3338
Fragments arranged by size:
3338 1572 584
ApaLI G'TGCA_C
Cuts at: 3551 4707 3551
Size: 1156 4338
ApoI r'AATT_y
Cuts at: 1493 2198 2803 3029 3181 5290 1493
Size: 705 605 226 152 2109 1697
Fragments arranged by size:
2109 1697 705 605 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4048 160
Size: 872 3016 1606
Fragments arranged by size:
3016 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
AvaI C'yCGr_G
Cuts at: 1870 1943 2819 1870
Size: 73 876 4545
Fragments arranged by size:
4545 876 73
BamHI G'GATC_C
Cuts at: 2167 2824 3191 2167
Size: 657 367 4470
Fragments arranged by size:
4470 657 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2815 4270 618
Size: 325 217 627 1028 1455 1842
Fragments arranged by size:
1842 1455 1028 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2037 2602 2610 2813 1391
Size: 646 565 8 203 4072
Fragments arranged by size:
4072 646 565 203 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3685 3719 1903
Size: 34 1748 34 3678
Fragments arranged by size:
3678 1748 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3386 4596 3386
Size: 1210 4284
BclI T'GATC_A
Cuts at: 1548 2007 2715 1548
Size: 459 708 4327
Fragments arranged by size:
4327 708 459
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4105 136
Size: 107 122 71 631 605 2433 1525
Fragments arranged by size:
2433 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5489 5489
Size: 5494
BlpI GC'TnA_GC
Cuts at: 2036 2911 2036
Size: 875 4619
Bme1580I G_kGCm'C
Cuts at: 1790 3555 4711 1790
Size: 1765 1156 2573
Fragments arranged by size:
2573 1765 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5494
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3225 4183 442
Size: 416 53 83 399 24 1808 958 1753
Fragments arranged by size:
1808 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4154 1239
Size: 2915 2579
Bpu10I CC'TnA_GC
Cuts at: 2604 2604
Size: 5494
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3619 4484 4782 3619
Size: 865 298 4331
Fragments arranged by size:
4331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4157 882
Size: 617 2658 2219
Fragments arranged by size:
2658 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2448 3285 493
Size: 711 1244 837 2702
Fragments arranged by size:
2702 1244 837 711
BsaBI GATnn'nnATC
Cuts at: 5488 5488
Size: 5494
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3301 3683 4318 275
Size: 53 83 186 936 1768 382 635 1451
Fragments arranged by size:
1768 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3927 4325 4599 4746 5157 3927
Size: 398 274 147 411 4264
Fragments arranged by size:
4264 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2120 2150 1178
Size: 30 165 30 717 30 4522
Fragments arranged by size:
4522 717 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2189 2742 1186
Size: 1003 553 3938
Fragments arranged by size:
3938 1003 553
BseYI C'CCAG_C
Cuts at: 2029 2105 2163 2307 2502 4697 5193 2029
Size: 76 58 144 195 2195 496 2330
Fragments arranged by size:
2330 2195 496 195 144 76 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2032 1163
Size: 573 296 4625
Fragments arranged by size:
4625 573 296
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2944 3705 4733 1540
Size: 275 186 943 761 1028 2301
Fragments arranged by size:
2301 1028 943 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2592 3555 3640 4711 2592
Size: 963 85 1071 3375
Fragments arranged by size:
3375 1071 963 85
BsmI GAATG_Cn'
Cuts at: 3005 3098 3005
Size: 93 5401
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2592 2602 2610 2813 3555 3640
Size: 399 247 555 10 8 203 742 85
Cuts at: 3640 4711 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 555 399 247 203 85
10 8
BspEI T'CCGG_A
Cuts at: 5157 5157
Size: 5494
BspHI T'CATG_A
Cuts at: 3383 5113 3383
Size: 1730 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2048 2168 2849 844
Size: 1204 120 681 3489
Fragments arranged by size:
3489 1204 681 120
BsrBI CCG'CTC
Cuts at: 1394 1823 3381 1394
Size: 429 1558 3507
Fragments arranged by size:
3507 1558 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2284 3989 4163 65
Size: 1183 1036 1705 174 1396
Fragments arranged by size:
1705 1396 1183 1036 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4138 4325 1700
Size: 110 2328 187 2869
Fragments arranged by size:
2869 2328 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5494
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5494
BssSI C'ACGA_G
Cuts at: 2383 3554 4566 2383
Size: 1171 1012 3311
Fragments arranged by size:
3311 1171 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2272 794
Size: 1478 4016
BstBI TT'CG_AA
Cuts at: 4298 4298
Size: 5494
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5494
BstXI CCAn_nnnn'nTGG
Cuts at: 2510 2510
Size: 5494
BstYI r'GATC_y
Cuts at: 1972 2149 2167 2824 3191 3576 3593 5045 5131
Size: 177 18 657 367 385 17 1452 86
Cuts at: 5131 5143 5489 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 657 385 367 346 177 86
18 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4942
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4755
Fragments arranged by size:
4755 683 56
BtsI GCAGTG_nn'
Cuts at: 2491 2679 3010 3804 3824 2491
Size: 188 331 794 20 4161
Fragments arranged by size:
4161 794 331 188 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5494
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5459
DraI TTT'AAA
Cuts at: 2798 2798
Size: 5494
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4501 809
Size: 507 3185 1802
Fragments arranged by size:
3185 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2342 2734 2941
Size: 54 1425 50 275 18 512 392 207
Cuts at: 2941 3830 5459 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 512 392 275 207 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2941 1537
Size: 275 1129 4090
Fragments arranged by size:
4090 1129 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2760 3424 1353
Size: 1407 664 3423
Fragments arranged by size:
3423 1407 664
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2740 4081 4455 4601 387
Size: 1064 138 1151 1341 374 146 1280
Fragments arranged by size:
1341 1280 1151 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 2675 3557 4154 4941 5209 1239
Size: 393 638 405 882 597 787 268 1524
Fragments arranged by size:
1524 882 787 638 597 405 393 268
EcoRI G'AATT_C
Cuts at: 2803 3181 2803
Size: 378 5116
FspI TGC'GCA
Cuts at: 2276 2490 4000 2276
Size: 214 1510 3770
Fragments arranged by size:
3770 1510 214
FspAI rTGC'GCAy
Cuts at: 2490 2490
Size: 5494
HaeII r_GCGC'y
Cuts at: 1678 2272 4641 1678
Size: 594 2369 2531
Fragments arranged by size:
2531 2369 594
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4135 4167 4209 4241 1373
Size: 32 135 32 2563 32 42 32 2626
Fragments arranged by size:
2626 2563 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2838 3084 669
Size: 988 281 900 246 3079
Fragments arranged by size:
3079 988 900 281 246
HindIII A'AGCT_T
Cuts at: 748 2465 748
Size: 1717 3777
HpaI GTT'AAC
Cuts at: 3084 3084
Size: 5494
KpnI G_GTAC'C
Cuts at: 2819 2819
Size: 5494
MfeI C'AATT_G
Cuts at: 3093 3093
Size: 5494
MluI A'CGCG_T
Cuts at: 5370 5370
Size: 5494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2076 4608 4792 1305
Size: 771 2532 184 2007
Fragments arranged by size:
2532 2007 771 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2736 10
Size: 54 1425 343 904 2768
Fragments arranged by size:
2768 1425 904 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2261 2484 2579 3452 3811 3970 518
Size: 648 1095 223 95 873 359 159 2042
Fragments arranged by size:
2042 1095 873 648 359 223 159 95
MspA1I CmG'CkG
Cuts at: 1606 2023 2721 2910 3587 4735 4980 1606
Size: 417 698 189 677 1148 245 2120
Fragments arranged by size:
2120 1148 698 677 417 245 189
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4942
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5494
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5494
NotI GC'GGCC_GC
Cuts at: 2941 2941
Size: 5494
NspI r_CATG'y
Cuts at: 1132 1229 2852 4397 1132
Size: 97 1623 1545 2229
Fragments arranged by size:
2229 1623 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5494
PciI A'CATG_T
Cuts at: 4393 4393
Size: 5494
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5494
PmeI GTTT'AAAC
Cuts at: 2798 2798
Size: 5494
PmlI CAC'GTG
Cuts at: 2448 2448
Size: 5494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 5076 5108 3563
Size: 32 1481 32 3949
Fragments arranged by size:
3949 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5494
PsiI TTA'TAA
Cuts at: 3064 3064
Size: 5494
PstI C_TGCA'G
Cuts at: 830 2674 2846 830
Size: 1844 172 3478
Fragments arranged by size:
3478 1844 172
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5494
PvuII CAG'CTG
Cuts at: 2721 2721
Size: 5494
SalI G'TCGA_C
Cuts at: 1936 2836 1936
Size: 900 4594
SbfI CC_TGCA'GG
Cuts at: 2846 2846
Size: 5494
ScaI AGT'ACT
Cuts at: 3742 5454 3742
Size: 1712 3782
SfcI C'TryA_G
Cuts at: 826 1045 2670 2842 3977 4658 4849 826
Size: 219 1625 172 1135 681 191 1471
Fragments arranged by size:
1625 1471 1135 681 219 191 172
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5494
SmaI CCC'GGG
Cuts at: 1872 2821 1872
Size: 949 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3598 4499 4761 820
Size: 197 926 1655 901 262 1553
Fragments arranged by size:
1655 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3285 493
Size: 2792 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5494
SphI G_CATG'C
Cuts at: 1132 2852 1132
Size: 1720 3774
SspI AAT'ATT
Cuts at: 5 52 3418 5
Size: 47 3366 2081
Fragments arranged by size:
3366 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5494
StyI C'CwwG_G
Cuts at: 513 1065 2933 513
Size: 552 1868 3074
Fragments arranged by size:
3074 1868 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2321 3521 3538 3691 3876 4321 325
Size: 1255 741 1200 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1200 741 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3740 5452 96
Size: 274 80 33 51 1443 1763 1712 138
Fragments arranged by size:
1763 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2740 2772 5454 5486 2740
Size: 32 2682 32 2748
Fragments arranged by size:
2748 2682 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5494
XbaI T'CTAG_A
Cuts at: 2830 2830
Size: 5494
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5494
XmaI C'CCGG_G
Cuts at: 1870 2819 1870
Size: 949 4545
XmnI GAAnn'nnTTC
Cuts at: 3623 3623
Size: 5494
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3302 276
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI