Restriction map for
pFN21AE2222
(Circular) (Six-base) MAPSORT of: pFN21AE2222.seq Check: 3663 from: 1 to: 5443
pFN21AE2222 5443 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3253 278
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2786 1937
Size: 849 4594
Acc65I G'GTAC_C
Cuts at: 2764 2764
Size: 5443
AclI AA'CG_TT
Cuts at: 1563 3570 3943 1563
Size: 2007 373 3063
Fragments arranged by size:
3063 2007 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2685 3506 4890 5158 1632
Size: 1053 821 1384 268 1917
Fragments arranged by size:
1917 1384 1053 821 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5246
AflIII A'CryG_T
Cuts at: 1203 4342 5319 1203
Size: 3139 977 1327
Fragments arranged by size:
3139 1327 977
AgeI A'CCGG_T
Cuts at: 2160 4274 2160
Size: 2114 3329
AhdI GACnn_n'nnGTC
Cuts at: 4172 4172
Size: 5443
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5443
AlwNI CAG_nnn'CTG
Cuts at: 2093 2212 2252 3186 4758 2093
Size: 119 40 934 1572 2778
Fragments arranged by size:
2778 1572 934 119 40
ApaLI G'TGCA_C
Cuts at: 3500 4656 3500
Size: 1156 4287
ApoI r'AATT_y
Cuts at: 1493 2636 2752 2978 3130 5239 1493
Size: 1143 116 226 152 2109 1697
Fragments arranged by size:
2109 1697 1143 226 152 116
AseI AT'TA_AT
Cuts at: 160 1032 3997 160
Size: 872 2965 1606
Fragments arranged by size:
2965 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
AvaI C'yCGr_G
Cuts at: 1870 1943 2401 2423 2768 1870
Size: 73 458 22 345 4545
Fragments arranged by size:
4545 458 345 73 22
BamHI G'GATC_C
Cuts at: 2773 3140 2773
Size: 367 5076
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2202 2470 2764 4219 618
Size: 325 217 627 415 268 294 1455 1842
Fragments arranged by size:
1842 1455 627 415 325 294 268 217
BanII G_rGCy'C
Cuts at: 1391 2762 1391
Size: 1371 4072
BbeI G_GCGC'C
Cuts at: 2474 2474
Size: 5443
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3634 3668 1903
Size: 34 1697 34 3678
Fragments arranged by size:
3678 1697 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3335 4545 3335
Size: 1210 4233
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4054 136
Size: 107 122 71 631 605 2382 1525
Fragments arranged by size:
2382 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2338 5438 2338
Size: 3100 2343
BlpI GC'TnA_GC
Cuts at: 2094 2860 2094
Size: 766 4677
Bme1580I G_kGCm'C
Cuts at: 1790 2384 2732 3504 4660 1790
Size: 594 348 772 1156 2573
Fragments arranged by size:
2573 1156 772 594 348
BmgBI CAC'GTC
Cuts at: 1318 2392 1318
Size: 1074 4369
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3174 4132 442
Size: 416 53 83 399 24 1757 958 1753
Fragments arranged by size:
1757 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2281 4103 1239
Size: 968 74 1822 2579
Fragments arranged by size:
2579 1822 968 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3568 4433 4731 3568
Size: 865 298 4280
Fragments arranged by size:
4280 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4106 882
Size: 617 2607 2219
Fragments arranged by size:
2607 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3234 493
Size: 711 2030 2702
Fragments arranged by size:
2702 2030 711
BsaBI GATnn'nnATC
Cuts at: 5437 5437
Size: 5443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2471 3250 3632 4267
Size: 53 83 186 936 938 779 382 635
Cuts at: 4267 275
Size: 1451
Fragments arranged by size:
1451 938 936 779 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2160 3876 4274 4548 4695 5106 2160
Size: 1716 398 274 147 411 2497
Fragments arranged by size:
2497 1716 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5218
Fragments arranged by size:
5218 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2635 1186
Size: 1449 3994
BseYI C'CCAG_C
Cuts at: 2306 4646 5142 2306
Size: 2340 496 2607
Fragments arranged by size:
2607 2340 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2297 1163
Size: 573 561 4309
Fragments arranged by size:
4309 573 561
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2893 3654 4682 1540
Size: 275 186 892 761 1028 2301
Fragments arranged by size:
2301 1028 892 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 3504 3589 4660 2100
Size: 1404 85 1071 2883
Fragments arranged by size:
2883 1404 1071 85
BsmI GAATG_Cn'
Cuts at: 2954 3047 2954
Size: 93 5350
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2384 2732 2762 3504 3589 4660
Size: 399 310 284 348 30 742 85 1071
Cuts at: 4660 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 348 310 284 85
30
BspEI T'CCGG_A
Cuts at: 5106 5106
Size: 5443
BspHI T'CATG_A
Cuts at: 2503 3332 5062 2503
Size: 829 1730 2884
Fragments arranged by size:
2884 1730 829
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2731 2798 844
Size: 1887 67 3489
Fragments arranged by size:
3489 1887 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3330 1394
Size: 429 1507 3507
Fragments arranged by size:
3507 1507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3938 4112 65
Size: 1183 2690 174 1396
Fragments arranged by size:
2690 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2160 4087 4274 1700
Size: 110 350 1927 187 2869
Fragments arranged by size:
2869 1927 350 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5443
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5443
BssSI C'ACGA_G
Cuts at: 3503 4515 3503
Size: 1012 4431
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5443
BstBI TT'CG_AA
Cuts at: 4247 4247
Size: 5443
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5443
BstXI CCAn_nnnn'nTGG
Cuts at: 2063 2063
Size: 5443
BstYI r'GATC_y
Cuts at: 1972 2302 2338 2773 3140 3525 3542 4994 5080
Size: 330 36 435 367 385 17 1452 86
Cuts at: 5080 5092 5438 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 435 385 367 346 330 86
36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2716 513
Size: 552 939 712 3240
Fragments arranged by size:
3240 939 712 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4704
Fragments arranged by size:
4704 683 56
BtsI GCAGTG_nn'
Cuts at: 2959 3753 3773 2959
Size: 794 20 4629
Fragments arranged by size:
4629 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5408
DraI TTT'AAA
Cuts at: 2747 2747
Size: 5443
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4450 809
Size: 507 3134 1802
Fragments arranged by size:
3134 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2703 2890 3779
Size: 54 1425 50 275 18 873 187 889
Cuts at: 3779 5408 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 873 275 187 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2890 1537
Size: 275 1078 4090
Fragments arranged by size:
4090 1078 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3373 1353
Size: 2020 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4030 4404 4550 387
Size: 1064 138 2441 374 146 1280
Fragments arranged by size:
2441 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2281 2685 3506 4103 4890 5158
Size: 393 575 74 404 821 597 787 268
Cuts at: 5158 1239
Size: 1524
Fragments arranged by size:
1524 821 787 597 575 404 393 268
74
EcoO109I rG'GnC_Cy
Cuts at: 2125 2256 2494 2125
Size: 131 238 5074
Fragments arranged by size:
5074 238 131
EcoRI G'AATT_C
Cuts at: 2636 2752 3130 2636
Size: 116 378 4949
Fragments arranged by size:
4949 378 116
FspI TGC'GCA
Cuts at: 3949 3949
Size: 5443
HaeII r_GCGC'y
Cuts at: 1678 2094 2175 2474 4590 1678
Size: 416 81 299 2116 2531
Fragments arranged by size:
2531 2116 416 299 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2101 2133 2294 2326 4084
Size: 32 135 32 529 32 161 32 1758
Cuts at: 4084 4116 4158 4190 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1758 529 161 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2287 2787 3033 669
Size: 988 281 349 500 246 3079
Fragments arranged by size:
3079 988 500 349 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5443
HpaI GTT'AAC
Cuts at: 3033 3033
Size: 5443
KasI G'GCGC_C
Cuts at: 2470 2470
Size: 5443
KpnI G_GTAC'C
Cuts at: 2768 2768
Size: 5443
MfeI C'AATT_G
Cuts at: 3042 3042
Size: 5443
MluI A'CGCG_T
Cuts at: 5319 5319
Size: 5443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4557 4741 1305
Size: 3252 184 2007
Fragments arranged by size:
3252 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2705 10
Size: 54 1425 343 873 2748
Fragments arranged by size:
2748 1425 873 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3401 3760 3919 518
Size: 648 2235 359 159 2042
Fragments arranged by size:
2235 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2859 3536 4684 4929 1606
Size: 1253 677 1148 245 2120
Fragments arranged by size:
2120 1253 1148 677 245
NarI GG'CG_CC
Cuts at: 2471 2471
Size: 5443
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3952
Fragments arranged by size:
3952 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5443
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5443
NotI GC'GGCC_GC
Cuts at: 2890 2890
Size: 5443
NspI r_CATG'y
Cuts at: 1132 1229 2801 4346 1132
Size: 97 1572 1545 2229
Fragments arranged by size:
2229 1572 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5443
PciI A'CATG_T
Cuts at: 4342 4342
Size: 5443
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5443
PfoI T'CCnGG_A
Cuts at: 2184 2490 2184
Size: 306 5137
PmeI GTTT'AAAC
Cuts at: 2747 2747
Size: 5443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3512 3544 5025 5057 3512
Size: 32 1481 32 3898
Fragments arranged by size:
3898 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2494 2125
Size: 369 5074
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5443
PsiI TTA'TAA
Cuts at: 3013 3013
Size: 5443
PspXI vC'TCGA_Gb
Cuts at: 2423 2423
Size: 5443
PstI C_TGCA'G
Cuts at: 830 2795 830
Size: 1965 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5443
SalI G'TCGA_C
Cuts at: 1936 2785 1936
Size: 849 4594
SanDI GG'GwC_CC
Cuts at: 2125 2125
Size: 5443
SbfI CC_TGCA'GG
Cuts at: 2795 2795
Size: 5443
ScaI AGT'ACT
Cuts at: 3691 5403 3691
Size: 1712 3731
SfcI C'TryA_G
Cuts at: 826 1045 2791 3926 4607 4798 826
Size: 219 1746 1135 681 191 1471
Fragments arranged by size:
1746 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2472 2472
Size: 5443
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5443
SmaI CCC'GGG
Cuts at: 1872 2770 1872
Size: 898 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2423 3547 4448 4710 820
Size: 197 926 480 1124 901 262 1553
Fragments arranged by size:
1553 1124 926 901 480 262 197
SnaBI TAC'GTA
Cuts at: 493 3234 493
Size: 2741 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5443
SphI G_CATG'C
Cuts at: 1132 2801 1132
Size: 1669 3774
SspI AAT'ATT
Cuts at: 5 52 3367 5
Size: 47 3315 2081
Fragments arranged by size:
3315 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5443
StyI C'CwwG_G
Cuts at: 513 1065 2004 2882 513
Size: 552 939 878 3074
Fragments arranged by size:
3074 939 878 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3470 3487 3640 3825 4270 325
Size: 1255 1890 17 153 185 445 1498
Fragments arranged by size:
1890 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2694 3689 5401
Size: 274 80 33 51 1443 717 995 1712
Cuts at: 5401 96
Size: 138
Fragments arranged by size:
1712 1443 995 717 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2403 2435 5403 5435 2403
Size: 32 2968 32 2411
Fragments arranged by size:
2968 2411 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5443
XbaI T'CTAG_A
Cuts at: 2779 2779
Size: 5443
XhoI C'TCGA_G
Cuts at: 1943 2423 1943
Size: 480 4963
XmaI C'CCGG_G
Cuts at: 1870 2768 1870
Size: 898 4545
XmnI GAAnn'nnTTC
Cuts at: 3572 3572
Size: 5443
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3251 276
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII
SacI SacII SapI SexAI SfiI SrfI SwaI XcmI