Restriction map for
pFN21AE2221
(Circular) (Six-base) MAPSORT of: pFN21AE2221.seq Check: 4757 from: 1 to: 5710
pFN21AE2221 5710 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2675 3053 1937
Size: 738 378 4594
Fragments arranged by size:
4594 738 378
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 2314 3773 5157 5425 1632
Size: 492 190 1459 1384 268 1917
Fragments arranged by size:
1917 1459 1384 492 268 190
AfeI AGC'GCT
Cuts at: 2391 2391
Size: 5710
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 2706 4609 5586 1203
Size: 1503 1903 977 1327
Fragments arranged by size:
1903 1503 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 2559 4439 2559
Size: 1880 3830
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5710
AlwNI CAG_nnn'CTG
Cuts at: 3453 5025 3453
Size: 1572 4138
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 3019 3245 3397 5506 1493
Size: 1526 226 152 2109 1697
Fragments arranged by size:
2109 1697 1526 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 2646 3035 1870
Size: 73 703 389 4545
Fragments arranged by size:
4545 703 389 73
BamHI G'GATC_C
Cuts at: 2718 2854 2962 3040 3407 2718
Size: 136 108 78 367 5021
Fragments arranged by size:
5021 367 136 108 78
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3031 4486 618
Size: 325 217 627 1244 1455 1842
Fragments arranged by size:
1842 1455 1244 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2412 2809 3029 1391
Size: 1021 397 220 4072
Fragments arranged by size:
4072 1021 397 220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2130 928
Size: 971 231 4508
Fragments arranged by size:
4508 971 231
BbvCI CC'TCA_GC
Cuts at: 2106 2106
Size: 5710
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3901 3935 1903
Size: 34 1964 34 3678
Fragments arranged by size:
3678 1964 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BfrBI ATG'CAT
Cuts at: 2984 2984
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4321 136
Size: 107 122 71 631 605 2649 1525
Fragments arranged by size:
2649 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5705 5705
Size: 5710
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 2073 3771 4927 1790
Size: 283 1698 1156 2573
Fragments arranged by size:
2573 1698 1156 283
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2654 3441 4399
Size: 416 53 83 399 24 1237 787 958
Cuts at: 4399 442
Size: 1753
Fragments arranged by size:
1753 1237 958 787 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2039 2039
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2396 4370 1239
Size: 1157 1974 2579
Fragments arranged by size:
2579 1974 1157
Bpu10I CC'TnA_GC
Cuts at: 2106 2463 2106
Size: 357 5353
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2305 3835 4700 4998 2305
Size: 1530 865 298 3017
Fragments arranged by size:
3017 1530 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2338 2657 4373 882
Size: 617 839 319 1716 2219
Fragments arranged by size:
2219 1716 839 617 319
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3517 3899 4534 275
Size: 53 83 186 936 1984 382 635 1451
Fragments arranged by size:
1984 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4143 4541 4815 4962 5373 4143
Size: 398 274 147 411 4480
Fragments arranged by size:
4480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2442 2472 2556 2586 1178
Size: 30 165 30 1039 30 84 30 4302
Fragments arranged by size:
4302 1039 165 84 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5710
BseYI C'CCAG_C
Cuts at: 2796 4913 5409 2796
Size: 2117 496 3097
Fragments arranged by size:
3097 2117 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2075 1163
Size: 573 339 4798
Fragments arranged by size:
4798 573 339
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2809 3771 3856 4927 2809
Size: 962 85 1071 3592
Fragments arranged by size:
3592 1071 962 85
BsmI GAATG_Cn'
Cuts at: 2986 3221 3314 2986
Size: 235 93 5382
Fragments arranged by size:
5382 235 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2412 2809 3029 3771 3856 4927
Size: 399 283 339 397 220 742 85 1071
Cuts at: 4927 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 397 339 283 220
85
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 2440 3599 5329 2440
Size: 1159 1730 2821
Fragments arranged by size:
2821 1730 1159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2097 3065 844
Size: 1253 968 3489
Fragments arranged by size:
3489 1253 968
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4205 4379 65
Size: 1183 2957 174 1396
Fragments arranged by size:
2957 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4354 4541 1700
Size: 110 2544 187 2869
Fragments arranged by size:
2869 2544 187 110
BsrGI T'GTAC_A
Cuts at: 96 2889 96
Size: 2793 2917
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 3770 4782 3770
Size: 1012 4698
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstXI CCAn_nnnn'nTGG
Cuts at: 2383 2383
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 2718 2854 2962 3040 3407 3792 3809 5261
Size: 746 136 108 78 367 385 17 1452
Cuts at: 5261 5347 5359 5705 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 746 385 367 346 136 108
86 78 17 12
BstZ17I GTA'TAC
Cuts at: 2676 2676
Size: 5710
BtgI C'CryG_G
Cuts at: 513 1065 2004 2906 2967 513
Size: 552 939 902 61 3256
Fragments arranged by size:
3256 939 902 552 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 3226 4020 4040 3226
Size: 794 20 4896
Fragments arranged by size:
4896 794 20
ClaI AT'CG_AT
Cuts at: 1554 2494 1554
Size: 940 4770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 3157 4046
Size: 54 1425 50 275 18 234 1093 889
Cuts at: 4046 5675 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1093 889 275 234 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3640 1353
Size: 2287 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2807 2807
Size: 5710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2314 2396 3773 4370 5157 5425
Size: 393 492 190 82 1377 597 787 268
Cuts at: 5425 1239
Size: 1524
Fragments arranged by size:
1524 1377 787 597 492 393 268 190
82
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 2393 2797 4857 1678
Size: 715 404 2060 2531
Fragments arranged by size:
2531 2060 715 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2157 2189 2278 2310 2335
Size: 32 135 32 585 32 89 32 25
Cuts at: 2335 2367 2509 2541 2545 2577 4351 4383 4425
Size: 32 142 32 4 32 1774 32 42
Cuts at: 4425 4457 1373
Size: 32 2626
Fragments arranged by size:
2626 1774 585 142 135 89 42 32
32 32 32 32 32 32 32 32
25 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2182 2264 4824 5008 1305
Size: 877 82 2560 184 2007
Fragments arranged by size:
2560 2007 877 184 82
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3888
Fragments arranged by size:
3888 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2445 3668 4027 4186 518
Size: 648 1279 1223 359 159 2042
Fragments arranged by size:
2042 1279 1223 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2908 3126 3803 4951 5196 1606
Size: 1302 218 677 1148 245 2120
Fragments arranged by size:
2120 1302 1148 677 245 218
NcoI C'CATG_G
Cuts at: 513 1065 2004 2967 513
Size: 552 939 963 3256
Fragments arranged by size:
3256 963 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NsiI A_TGCA'T
Cuts at: 2986 2986
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 2710 3068 4613 1132
Size: 97 1481 358 1545 2229
Fragments arranged by size:
2229 1545 1481 358 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5710
PciI A'CATG_T
Cuts at: 2706 4609 2706
Size: 1903 3807
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2666 1108
Size: 1558 4152
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PstI C_TGCA'G
Cuts at: 830 3062 830
Size: 2232 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
SacI G_AGCT'C
Cuts at: 2809 2809
Size: 5710
SacII CC_GC'GG
Cuts at: 2909 2909
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 2476 3058 4193 4874 5065 826
Size: 219 1431 582 1135 681 191 1471
Fragments arranged by size:
1471 1431 1135 681 582 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2284 2646 3814 4715 4977 820
Size: 197 926 341 362 1168 901 262 1553
Fragments arranged by size:
1553 1168 926 901 362 341 262 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2004 2967 3149 513
Size: 552 939 963 182 3074
Fragments arranged by size:
3074 963 939 552 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2183 2623 2788 3737 3754 3907 4092
Size: 1255 603 440 165 949 17 153 185
Cuts at: 4092 4537 325
Size: 445 1498
Fragments arranged by size:
1498 1255 949 603 445 440 185 165
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2763 2889 3956
Size: 274 80 33 51 1443 786 126 1067
Cuts at: 3956 5668 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1067 786 274 138 126 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2556 2588 5670 5702 2556
Size: 32 3082 32 2564
Fragments arranged by size:
3082 2564 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XhoI C'TCGA_G
Cuts at: 1943 2646 1943
Size: 703 5007
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 2783 3839 2783
Size: 1056 4654
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SacI SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI