Restriction map for
pFN21AE2217
(Circular) (Six-base) MAPSORT of: pFN21AE2217.seq Check: 172 from: 1 to: 5584
pFN21AE2217 5584 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3394 278
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2451 2927 1937
Size: 514 476 4594
Fragments arranged by size:
4594 514 476
Acc65I G'GTAC_C
Cuts at: 2905 2905
Size: 5584
AclI AA'CG_TT
Cuts at: 1563 3711 4084 1563
Size: 2148 373 3063
Fragments arranged by size:
3063 2148 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2340 3647 5031 5299 1632
Size: 708 1307 1384 268 1917
Fragments arranged by size:
1917 1384 1307 708 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5387
AflIII A'CryG_T
Cuts at: 1203 4483 5460 1203
Size: 3280 977 1327
Fragments arranged by size:
3280 1327 977
AgeI A'CCGG_T
Cuts at: 4415 4415
Size: 5584
AhdI GACnn_n'nnGTC
Cuts at: 4313 4313
Size: 5584
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5584
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5584
AlwNI CAG_nnn'CTG
Cuts at: 3327 4899 3327
Size: 1572 4012
ApaLI G'TGCA_C
Cuts at: 3641 4797 3641
Size: 1156 4428
ApoI r'AATT_y
Cuts at: 1493 2091 2727 2893 3119 3271 5380 1493
Size: 598 636 166 226 152 2109 1697
Fragments arranged by size:
2109 1697 636 598 226 166 152
AseI AT'TA_AT
Cuts at: 160 1032 4138 160
Size: 872 3106 1606
Fragments arranged by size:
3106 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
AvaI C'yCGr_G
Cuts at: 1870 1943 2909 1870
Size: 73 966 4545
Fragments arranged by size:
4545 966 73
BamHI G'GATC_C
Cuts at: 2693 2914 3281 2693
Size: 221 367 4996
Fragments arranged by size:
4996 367 221
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2596 2905 4360 618
Size: 325 217 627 809 309 1455 1842
Fragments arranged by size:
1842 1455 809 627 325 309 217
BanII G_rGCy'C
Cuts at: 1391 2903 1391
Size: 1512 4072
BbeI G_GCGC'C
Cuts at: 2600 2600
Size: 5584
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4613
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2207 2241 3775 3809 1903
Size: 34 270 34 1534 34 3678
Fragments arranged by size:
3678 1534 270 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2110 3476 4686 2110
Size: 1366 1210 3008
Fragments arranged by size:
3008 1366 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5584
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4195 136
Size: 107 122 71 631 605 2523 1525
Fragments arranged by size:
2523 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5579 5579
Size: 5584
BlpI GC'TnA_GC
Cuts at: 3001 3001
Size: 5584
Bme1580I G_kGCm'C
Cuts at: 1790 3645 4801 1790
Size: 1855 1156 2573
Fragments arranged by size:
2573 1855 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5584
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3315 4273 442
Size: 416 53 83 399 24 1898 958 1753
Fragments arranged by size:
1898 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5584
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2169 2169
Size: 5584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2366 2405 4244 1239
Size: 1127 39 1839 2579
Fragments arranged by size:
2579 1839 1127 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3709 4574 4872 3709
Size: 865 298 4421
Fragments arranged by size:
4421 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4247 882
Size: 617 2748 2219
Fragments arranged by size:
2748 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3375 493
Size: 711 2171 2702
Fragments arranged by size:
2702 2171 711
BsaBI GATnn'nnATC
Cuts at: 5578 5578
Size: 5584
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2597 3391 3773 4408
Size: 53 83 186 936 1064 794 382 635
Cuts at: 4408 275
Size: 1451
Fragments arranged by size:
1451 1064 936 794 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4017 4415 4689 4836 5247 4017
Size: 398 274 147 411 4354
Fragments arranged by size:
4354 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5359
Fragments arranged by size:
5359 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5584
BseYI C'CCAG_C
Cuts at: 2517 4787 5283 2517
Size: 2270 496 2818
Fragments arranged by size:
2818 2270 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2790 1163
Size: 573 1054 3957
Fragments arranged by size:
3957 1054 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2826 3034 3795 4823 1540
Size: 275 186 825 208 761 1028 2301
Fragments arranged by size:
2301 1028 825 761 275 208 186
BsiHKAI G_wGCw'C
Cuts at: 3645 3730 4801 3645
Size: 85 1071 4428
Fragments arranged by size:
4428 1071 85
BsmI GAATG_Cn'
Cuts at: 3095 3188 3095
Size: 93 5491
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2903 3645 3730 4801 1391
Size: 399 1113 742 85 1071 2174
Fragments arranged by size:
2174 1113 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5247 5247
Size: 5584
BspHI T'CATG_A
Cuts at: 3473 5203 3473
Size: 1730 3854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2939 844
Size: 2095 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3471 1394
Size: 429 1648 3507
Fragments arranged by size:
3507 1648 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4079 4253 65
Size: 1183 2831 174 1396
Fragments arranged by size:
2831 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4228 4415 1700
Size: 110 2418 187 2869
Fragments arranged by size:
2869 2418 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5584
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5584
BssSI C'ACGA_G
Cuts at: 2216 3644 4656 2216
Size: 1428 1012 3144
Fragments arranged by size:
3144 1428 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5584
BstBI TT'CG_AA
Cuts at: 4388 4388
Size: 5584
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5584
BstYI r'GATC_y
Cuts at: 1972 2693 2703 2914 3281 3666 3683 5135 5221
Size: 721 10 211 367 385 17 1452 86
Cuts at: 5221 5233 5579 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 721 385 367 346 211 86
17 12 10
Bsu36I CC'TnA_GG
Cuts at: 2313 2313
Size: 5584
BtgI C'CryG_G
Cuts at: 513 1065 2424 2592 513
Size: 552 1359 168 3505
Fragments arranged by size:
3505 1359 552 168
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4845
Fragments arranged by size:
4845 683 56
BtsI GCAGTG_nn'
Cuts at: 3100 3894 3914 3100
Size: 794 20 4770
Fragments arranged by size:
4770 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5584
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2842 2877 529
Size: 35 2278 35 3236
Fragments arranged by size:
3236 2278 35 35
DraI TTT'AAA
Cuts at: 2743 2888 2743
Size: 145 5439
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4591 809
Size: 507 3275 1802
Fragments arranged by size:
3275 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3031 3920 5549
Size: 54 1425 50 275 18 1201 889 1629
Cuts at: 5549 8
Size: 43
Fragments arranged by size:
1629 1425 1201 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3031 1537
Size: 275 1219 4090
Fragments arranged by size:
4090 1219 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3514 1353
Size: 2161 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4171 4545 4691 387
Size: 1064 138 2582 374 146 1280
Fragments arranged by size:
2582 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2340 2366 2405 3647 4244 5031 5299
Size: 393 708 26 39 1242 597 787 268
Cuts at: 5299 1239
Size: 1524
Fragments arranged by size:
1524 1242 787 708 597 393 268 39
26
EcoO109I rG'GnC_Cy
Cuts at: 2865 2865
Size: 5584
EcoRI G'AATT_C
Cuts at: 2893 3271 2893
Size: 378 5206
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2391 2423 2650 2391
Size: 32 227 5325
Fragments arranged by size:
5325 227 32
FspI TGC'GCA
Cuts at: 4090 4090
Size: 5584
HaeII r_GCGC'y
Cuts at: 1678 2600 4731 1678
Size: 922 2131 2531
Fragments arranged by size:
2531 2131 922
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2227 2259 4225 4257 4299
Size: 32 135 32 655 32 1966 32 42
Cuts at: 4299 4331 1373
Size: 32 2626
Fragments arranged by size:
2626 1966 655 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2452 2928 3174 669
Size: 988 281 514 476 246 3079
Fragments arranged by size:
3079 988 514 476 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5584
HpaI GTT'AAC
Cuts at: 3174 3174
Size: 5584
KasI G'GCGC_C
Cuts at: 2596 2596
Size: 5584
KpnI G_GTAC'C
Cuts at: 2909 2909
Size: 5584
MfeI C'AATT_G
Cuts at: 3183 3183
Size: 5584
MluI A'CGCG_T
Cuts at: 5460 5460
Size: 5584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4698 4882 1305
Size: 3393 184 2007
Fragments arranged by size:
3393 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3762
Fragments arranged by size:
3762 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2179 3542 3901 4060 518
Size: 648 1013 1363 359 159 2042
Fragments arranged by size:
2042 1363 1013 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2344 2521 2594 3000 3677 4825 5070 1606
Size: 738 177 73 406 677 1148 245 2120
Fragments arranged by size:
2120 1148 738 677 406 245 177 73
NarI GG'CG_CC
Cuts at: 2597 2597
Size: 5584
NcoI C'CATG_G
Cuts at: 513 1065 2424 513
Size: 552 1359 3673
Fragments arranged by size:
3673 1359 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5584
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5584
NotI GC'GGCC_GC
Cuts at: 3031 3031
Size: 5584
NspI r_CATG'y
Cuts at: 1132 1229 2338 2942 4487 1132
Size: 97 1109 604 1545 2229
Fragments arranged by size:
2229 1545 1109 604 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5584
PciI A'CATG_T
Cuts at: 4483 4483
Size: 5584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5584
PmeI GTTT'AAAC
Cuts at: 2888 2888
Size: 5584
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2572 2604 3653 3685 5166 5198 2572
Size: 32 1049 32 1481 32 2958
Fragments arranged by size:
2958 1481 1049 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2865 2865
Size: 5584
PshAI GACnn'nnGTC
Cuts at: 1613 2870 1613
Size: 1257 4327
PsiI TTA'TAA
Cuts at: 3154 3154
Size: 5584
PstI C_TGCA'G
Cuts at: 830 2936 830
Size: 2106 3478
PvuI CG_AT'CG
Cuts at: 2001 2826 2001
Size: 825 4759
PvuII CAG'CTG
Cuts at: 2344 2521 2344
Size: 177 5407
SacII CC_GC'GG
Cuts at: 2595 2595
Size: 5584
SalI G'TCGA_C
Cuts at: 1936 2450 2926 1936
Size: 514 476 4594
Fragments arranged by size:
4594 514 476
SanDI GG'GwC_CC
Cuts at: 2865 2865
Size: 5584
SbfI CC_TGCA'GG
Cuts at: 2936 2936
Size: 5584
ScaI AGT'ACT
Cuts at: 2258 3832 5544 2258
Size: 1574 1712 2298
Fragments arranged by size:
2298 1712 1574
SfcI C'TryA_G
Cuts at: 826 1045 2932 4067 4748 4939 826
Size: 219 1887 1135 681 191 1471
Fragments arranged by size:
1887 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2598 2598
Size: 5584
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5584
SmaI CCC'GGG
Cuts at: 1872 2911 1872
Size: 1039 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3688 4589 4851 820
Size: 197 926 1745 901 262 1553
Fragments arranged by size:
1745 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3375 493
Size: 2882 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5584
SphI G_CATG'C
Cuts at: 1132 2942 1132
Size: 1810 3774
SspI AAT'ATT
Cuts at: 5 52 2800 3508 5
Size: 47 2748 708 2081
Fragments arranged by size:
2748 2081 708 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5584
StyI C'CwwG_G
Cuts at: 513 1065 2229 2424 3023 513
Size: 552 1164 195 599 3074
Fragments arranged by size:
3074 1164 599 552 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3611 3628 3781 3966 4411 325
Size: 1255 2031 17 153 185 445 1498
Fragments arranged by size:
2031 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2132 2256 3830
Size: 274 80 33 51 1443 155 124 1574
Cuts at: 3830 5542 96
Size: 1712 138
Fragments arranged by size:
1712 1574 1443 274 155 138 124 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2204 2236 5544 5576 2204
Size: 32 3308 32 2212
Fragments arranged by size:
3308 2212 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5584
XbaI T'CTAG_A
Cuts at: 2920 2920
Size: 5584
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2431 2431
Size: 5584
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5584
XmaI C'CCGG_G
Cuts at: 1870 2909 1870
Size: 1039 4545
XmnI GAAnn'nnTTC
Cuts at: 2403 2799 3713 2403
Size: 396 914 4274
Fragments arranged by size:
4274 914 396
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3392 276
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SapI SexAI SfiI SrfI SwaI