Restriction map for
pFN21AE2208
(Circular) (Six-base) MAPSORT of: pFN21AE2208.seq Check: 5593 from: 1 to: 5473
pFN21AE2208 5473 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3283 278
Size: 53 83 186 936 1747 2468
Fragments arranged by size:
2468 1747 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2816 1937
Size: 879 4594
Acc65I G'GTAC_C
Cuts at: 2794 2794
Size: 5473
AclI AA'CG_TT
Cuts at: 1563 3600 3973 1563
Size: 2037 373 3063
Fragments arranged by size:
3063 2037 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 2507 3536 4920 5188 1632
Size: 695 180 1029 1384 268 1917
Fragments arranged by size:
1917 1384 1029 695 268 180
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5276
AflIII A'CryG_T
Cuts at: 1203 4372 5349 1203
Size: 3169 977 1327
Fragments arranged by size:
3169 1327 977
AgeI A'CCGG_T
Cuts at: 4304 4304
Size: 5473
AhdI GACnn_n'nnGTC
Cuts at: 4202 4202
Size: 5473
AleI CACnn'nnGTG
Cuts at: 1166 2123 1166
Size: 957 4516
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5473
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2354 2386 2354
Size: 32 5441
AlwNI CAG_nnn'CTG
Cuts at: 2381 2716 2731 3216 4788 2381
Size: 335 15 485 1572 3066
Fragments arranged by size:
3066 1572 485 335 15
ApaLI G'TGCA_C
Cuts at: 2126 3530 4686 2126
Size: 1404 1156 2913
Fragments arranged by size:
2913 1404 1156
ApoI r'AATT_y
Cuts at: 1493 2582 2782 3008 3160 5269 1493
Size: 1089 200 226 152 2109 1697
Fragments arranged by size:
2109 1697 1089 226 200 152
AseI AT'TA_AT
Cuts at: 160 1032 4027 160
Size: 872 2995 1606
Fragments arranged by size:
2995 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
AvaI C'yCGr_G
Cuts at: 1870 1943 2537 2546 2798 1870
Size: 73 594 9 252 4545
Fragments arranged by size:
4545 594 252 73 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2003 2036 2003
Size: 33 5440
BamHI G'GATC_C
Cuts at: 2259 2803 3170 2259
Size: 544 367 4562
Fragments arranged by size:
4562 544 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2794 4249 618
Size: 325 217 627 1007 1455 1842
Fragments arranged by size:
1842 1455 1007 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2427 2792 1391
Size: 1036 365 4072
Fragments arranged by size:
4072 1036 365
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3664 3698 1903
Size: 34 50 34 1643 34 3678
Fragments arranged by size:
3678 1643 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2031 3365 4575 2031
Size: 1334 1210 2929
Fragments arranged by size:
2929 1334 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5473
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2079 4084 136
Size: 107 122 71 631 605 407 2005 1525
Fragments arranged by size:
2005 1525 631 605 407 122 107 71
BglII A'GATC_T
Cuts at: 2688 5468 2688
Size: 2780 2693
BlpI GC'TnA_GC
Cuts at: 2890 2890
Size: 5473
Bme1580I G_kGCm'C
Cuts at: 1790 2130 2202 3534 4690 1790
Size: 340 72 1332 1156 2573
Fragments arranged by size:
2573 1332 1156 340 72
BmgBI CAC'GTC
Cuts at: 1318 2197 1318
Size: 879 4594
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3204 4162 442
Size: 416 53 83 399 24 1787 958 1753
Fragments arranged by size:
1787 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 2648 4133 1239
Size: 881 528 1485 2579
Fragments arranged by size:
2579 1485 881 528
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2426 3598 4463 4761 2426
Size: 1172 865 298 3138
Fragments arranged by size:
3138 1172 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4136 882
Size: 617 2637 2219
Fragments arranged by size:
2637 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3264 493
Size: 711 2060 2702
Fragments arranged by size:
2702 2060 711
BsaBI GATnn'nnATC
Cuts at: 5467 5467
Size: 5473
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2114 3280 3662 4297
Size: 53 83 186 936 581 1166 382 635
Cuts at: 4297 275
Size: 1451
Fragments arranged by size:
1451 1166 936 635 581 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3906 4304 4578 4725 5136 3906
Size: 398 274 147 411 4243
Fragments arranged by size:
4243 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2078 2104 2108 2134 2279
Size: 30 165 30 675 26 4 26 145
Cuts at: 2279 2309 2447 2450 2477 2480 2594 2624 1178
Size: 30 138 3 27 3 114 30 4027
Fragments arranged by size:
4027 675 165 145 138 114 30 30
30 30 27 26 26 4 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2120 2612 2615 2645 2675 1186
Size: 931 3 492 3 30 30 3984
Fragments arranged by size:
3984 931 492 30 30 3 3
BseYI C'CCAG_C
Cuts at: 2229 2526 4676 5172 2229
Size: 297 2150 496 2530
Fragments arranged by size:
2530 2150 496 297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2516 2771 1163
Size: 573 780 255 3865
Fragments arranged by size:
3865 780 573 255
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2923 3684 4712 1540
Size: 275 186 922 761 1028 2301
Fragments arranged by size:
2301 1028 922 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2130 2468 3534 3619 4690 2130
Size: 338 1066 85 1071 2913
Fragments arranged by size:
2913 1071 1066 338 85
BsmI GAATG_Cn'
Cuts at: 2984 3077 2984
Size: 93 5380
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2202 2427 2468 2792 3534 3619
Size: 399 340 72 225 41 324 742 85
Cuts at: 3619 4690 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 340 324 225 85
72 41
BspEI T'CCGG_A
Cuts at: 5136 5136
Size: 5473
BspHI T'CATG_A
Cuts at: 3362 5092 3362
Size: 1730 3743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2097 2828 844
Size: 1253 731 3489
Fragments arranged by size:
3489 1253 731
BsrBI CCG'CTC
Cuts at: 1394 1823 2110 2551 3360 1394
Size: 429 287 441 809 3507
Fragments arranged by size:
3507 809 441 429 287
BsrDI GCAATG_nn'
Cuts at: 65 1248 3968 4142 65
Size: 1183 2720 174 1396
Fragments arranged by size:
2720 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2740 4117 4304 1700
Size: 110 930 1377 187 2869
Fragments arranged by size:
2869 1377 930 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5473
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5473
BssSI C'ACGA_G
Cuts at: 3533 4545 3533
Size: 1012 4461
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5473
BstBI TT'CG_AA
Cuts at: 4277 4277
Size: 5473
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5473
BstYI r'GATC_y
Cuts at: 1972 2259 2355 2688 2803 3170 3555 3572 5024
Size: 287 96 333 115 367 385 17 1452
Cuts at: 5024 5110 5122 5468 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 333 287 115
96 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4921
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4734
Fragments arranged by size:
4734 683 56
BtsI GCAGTG_nn'
Cuts at: 2989 3783 3803 2989
Size: 794 20 4659
Fragments arranged by size:
4659 794 20
ClaI AT'CG_AT
Cuts at: 1554 2409 1554
Size: 855 4618
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5438
DraI TTT'AAA
Cuts at: 2777 2777
Size: 5473
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4480 809
Size: 507 3164 1802
Fragments arranged by size:
3164 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 2767 2920
Size: 54 1425 50 275 18 250 687 153
Cuts at: 2920 3809 5438 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 687 275 250 153 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2920 1537
Size: 275 1108 4090
Fragments arranged by size:
4090 1108 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2750 3403 1353
Size: 1397 653 3423
Fragments arranged by size:
3423 1397 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2714 4060 4434 4580 387
Size: 1064 138 1125 1346 374 146 1280
Fragments arranged by size:
1346 1280 1125 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2327 2507 2648 3536 4133 4920
Size: 393 488 207 180 141 888 597 787
Cuts at: 4920 5188 1239
Size: 268 1524
Fragments arranged by size:
1524 888 787 597 488 393 268 207
180 141
EcoNI CCTnn'n_nnAGG
Cuts at: 2102 2102
Size: 5473
EcoO109I rG'GnC_Cy
Cuts at: 2458 2521 2542 2668 2458
Size: 63 21 126 5263
Fragments arranged by size:
5263 126 63 21
EcoRI G'AATT_C
Cuts at: 2782 3160 2782
Size: 378 5095
FspI TGC'GCA
Cuts at: 3979 3979
Size: 5473
HaeII r_GCGC'y
Cuts at: 1678 4620 1678
Size: 2942 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2104 2136 2279 2311 2450
Size: 32 135 32 532 32 143 32 139
Cuts at: 2450 2482 4114 4146 4188 4220 1373
Size: 32 1632 32 42 32 2626
Fragments arranged by size:
2626 1632 532 143 139 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2817 3063 669
Size: 988 281 879 246 3079
Fragments arranged by size:
3079 988 879 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5473
HpaI GTT'AAC
Cuts at: 3063 3063
Size: 5473
KpnI G_GTAC'C
Cuts at: 2798 2798
Size: 5473
MfeI C'AATT_G
Cuts at: 3072 3072
Size: 5473
MluI A'CGCG_T
Cuts at: 5349 5349
Size: 5473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4587 4771 1305
Size: 3282 184 2007
Fragments arranged by size:
3282 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2769 10
Size: 54 1425 343 937 2714
Fragments arranged by size:
2714 1425 937 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 3431 3790 3949 518
Size: 648 957 1308 359 159 2042
Fragments arranged by size:
2042 1308 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2179 2233 2329 2530 2716 2889 3566
Size: 444 129 54 96 201 186 173 677
Cuts at: 3566 4714 4959 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 444 245 201 186 173
129 96 54
NaeI GCC'GGC
Cuts at: 2742 2742
Size: 5473
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4921
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5473
NgoMIV G'CCGG_C
Cuts at: 2740 2740
Size: 5473
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5473
NotI GC'GGCC_GC
Cuts at: 2920 2920
Size: 5473
NspI r_CATG'y
Cuts at: 1132 1229 2831 4376 1132
Size: 97 1602 1545 2229
Fragments arranged by size:
2229 1602 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5473
PciI A'CATG_T
Cuts at: 4372 4372
Size: 5473
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5473
PfoI T'CCnGG_A
Cuts at: 2358 2358
Size: 5473
PmeI GTTT'AAAC
Cuts at: 2777 2777
Size: 5473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2327 2359 3542 3574 5055 5087 2327
Size: 32 1183 32 1481 32 2713
Fragments arranged by size:
2713 1481 1183 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2458 2521 2542 2668 2458
Size: 63 21 126 5263
Fragments arranged by size:
5263 126 63 21
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5473
PsiI TTA'TAA
Cuts at: 3043 3043
Size: 5473
PstI C_TGCA'G
Cuts at: 830 2049 2721 2825 830
Size: 1219 672 104 3478
Fragments arranged by size:
3478 1219 672 104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5473
PvuII CAG'CTG
Cuts at: 2050 2329 2530 2716 2050
Size: 279 201 186 4807
Fragments arranged by size:
4807 279 201 186
SalI G'TCGA_C
Cuts at: 1936 2815 1936
Size: 879 4594
SbfI CC_TGCA'GG
Cuts at: 2825 2825
Size: 5473
ScaI AGT'ACT
Cuts at: 3721 5433 3721
Size: 1712 3761
SfcI C'TryA_G
Cuts at: 826 1045 2045 2131 2347 2717 2821 3956 4637
Size: 219 1000 86 216 370 104 1135 681
Cuts at: 4637 4828 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1000 681 370 219 216 191
104 86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5473
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5473
SmaI CCC'GGG
Cuts at: 1872 2800 1872
Size: 928 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2441 3577 4478 4740 820
Size: 197 926 498 1136 901 262 1553
Fragments arranged by size:
1553 1136 926 901 498 262 197
SnaBI TAC'GTA
Cuts at: 493 3264 493
Size: 2771 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5473
SphI G_CATG'C
Cuts at: 1132 2831 1132
Size: 1699 3774
SspI AAT'ATT
Cuts at: 5 52 3397 5
Size: 47 3345 2081
Fragments arranged by size:
3345 2081 47
StuI AGG'CCT
Cuts at: 1517 2137 1517
Size: 620 4853
StyI C'CwwG_G
Cuts at: 513 1065 2912 513
Size: 552 1847 3074
Fragments arranged by size:
3074 1847 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2602 3500 3517 3670 3855 4300 325
Size: 1255 1022 898 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1022 898 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2574 3719 5431
Size: 274 80 33 51 1443 597 1145 1712
Cuts at: 5431 96
Size: 138
Fragments arranged by size:
1712 1443 1145 597 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2447 2479 2594 2626 5433 5465 2447
Size: 32 115 32 2807 32 2455
Fragments arranged by size:
2807 2455 115 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5473
XbaI T'CTAG_A
Cuts at: 2809 2809
Size: 5473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2395 2476 2395
Size: 81 5392
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5473
XmaI C'CCGG_G
Cuts at: 1870 2798 1870
Size: 928 4545
XmnI GAAnn'nnTTC
Cuts at: 3602 3602
Size: 5473
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3281 276
Size: 53 83 186 936 1747 2468
Fragments arranged by size:
2468 1747 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI