Restriction map for
pFN21AE2200
(Circular) (Six-base) MAPSORT of: pFN21AE2200.seq Check: 8657 from: 1 to: 6256
pFN21AE2200 6256 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3599 1937
Size: 1662 4594
Acc65I G'GTAC_C
Cuts at: 3577 3577
Size: 6256
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3073 3422 3433 4319 5703 5971 1632
Size: 1441 349 11 886 1384 268 1917
Fragments arranged by size:
1917 1441 1384 886 349 268 11
AfeI AGC'GCT
Cuts at: 2152 2152
Size: 6256
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6059
AflIII A'CryG_T
Cuts at: 1203 2024 3112 5155 6132 1203
Size: 821 1088 2043 977 1327
Fragments arranged by size:
2043 1327 1088 977 821
AgeI A'CCGG_T
Cuts at: 5087 5087
Size: 6256
AhdI GACnn_n'nnGTC
Cuts at: 4985 4985
Size: 6256
AleI CACnn'nnGTG
Cuts at: 1166 3252 1166
Size: 2086 4170
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6256
AlwNI CAG_nnn'CTG
Cuts at: 2473 3999 5571 2473
Size: 1526 1572 3158
Fragments arranged by size:
3158 1572 1526
ApaI G_GGCC'C
Cuts at: 2541 2541
Size: 6256
ApaLI G'TGCA_C
Cuts at: 4313 5469 4313
Size: 1156 5100
ApoI r'AATT_y
Cuts at: 1493 3565 3791 3943 6052 1493
Size: 2072 226 152 2109 1697
Fragments arranged by size:
2109 2072 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4810 160
Size: 872 3778 1606
Fragments arranged by size:
3778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 2110 2335 2621 3581 1870
Size: 73 167 225 286 960 4545
Fragments arranged by size:
4545 960 286 225 167 73
BamHI G'GATC_C
Cuts at: 2746 3586 3953 2746
Size: 840 367 5049
Fragments arranged by size:
5049 840 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2073 2135 2311 2791 3577
Size: 325 217 627 286 62 176 480 786
Cuts at: 3577 5032 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 786 627 480 325 286 217
176 62
BanII G_rGCy'C
Cuts at: 1391 2216 2541 2768 2972 3061 3575 1391
Size: 825 325 227 204 89 514 4072
Fragments arranged by size:
4072 825 514 325 227 204 89
BbeI G_GCGC'C
Cuts at: 2139 2315 2139
Size: 176 6080
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3079 3410 928
Size: 971 1180 331 3774
Fragments arranged by size:
3774 1180 971 331
BbvCI CC'TCA_GC
Cuts at: 2858 2858
Size: 6256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4447 4481 1903
Size: 34 50 34 2426 34 3678
Fragments arranged by size:
3678 2426 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4148 5358 4148
Size: 1210 5046
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6256
BfrBI ATG'CAT
Cuts at: 2913 3520 2913
Size: 607 5649
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2144 2763 4867
Size: 107 122 71 631 605 472 619 2104
Cuts at: 4867 136
Size: 1525
Fragments arranged by size:
2104 1525 631 619 605 472 122 107
71
BglII A'GATC_T
Cuts at: 6251 6251
Size: 6256
BlpI GC'TnA_GC
Cuts at: 3673 3673
Size: 6256
Bme1580I G_kGCm'C
Cuts at: 1790 2284 2541 4317 5473 1790
Size: 494 257 1776 1156 2573
Fragments arranged by size:
2573 1776 1156 494 257
BmgBI CAC'GTC
Cuts at: 1318 2221 3113 1318
Size: 903 892 4461
Fragments arranged by size:
4461 903 892
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3987 4945 442
Size: 416 53 83 399 24 2570 958 1753
Fragments arranged by size:
2570 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 2956 3141 3527 4916 1239
Size: 908 809 185 386 1389 2579
Fragments arranged by size:
2579 1389 908 809 386 185
Bpu10I CC'TnA_GC
Cuts at: 2474 2858 2474
Size: 384 5872
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4381 5246 5544 4381
Size: 865 298 5093
Fragments arranged by size:
5093 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3149 4919 882
Size: 617 1650 1770 2219
Fragments arranged by size:
2219 1770 1650 617
BsaAI yAC'GTr
Cuts at: 493 1204 2025 4047 493
Size: 711 821 2022 2702
Fragments arranged by size:
2702 2022 821 711
BsaBI GATnn'nnATC
Cuts at: 6250 6250
Size: 6256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 2136 2186 2312
Size: 53 83 186 936 509 94 50 126
Cuts at: 2312 2567 2678 2864 4063 4445 5080 275
Size: 255 111 186 1199 382 635 1451
Fragments arranged by size:
1451 1199 936 635 509 382 255 186
186 126 111 94 83 53 50
BsaWI w'CCGG_w
Cuts at: 4689 5087 5361 5508 5919 4689
Size: 398 274 147 411 5026
Fragments arranged by size:
5026 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 1178
Size: 30 165 30 624 30 5377
Fragments arranged by size:
5377 624 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2188 2477 2541 1186
Size: 1002 289 64 4901
Fragments arranged by size:
4901 1002 289 64
BseYI C'CCAG_C
Cuts at: 2439 2558 2612 2681 2770 2901 2963 5459 5955
Size: 119 54 69 89 131 62 2496 496
Cuts at: 5955 2439
Size: 2740
Fragments arranged by size:
2740 2496 496 131 119 89 69 62
54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2818 1163
Size: 573 1082 4601
Fragments arranged by size:
4601 1082 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3146 3706 4467 5495 1540
Size: 275 186 1145 560 761 1028 2301
Fragments arranged by size:
2301 1145 1028 761 560 275 186
BsiHKAI G_wGCw'C
Cuts at: 2972 4317 4402 5473 2972
Size: 1345 85 1071 3755
Fragments arranged by size:
3755 1345 1071 85
BsmI GAATG_Cn'
Cuts at: 2915 3096 3767 3860 2915
Size: 181 671 93 5311
Fragments arranged by size:
5311 671 181 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2049 2165 2049
Size: 116 6140
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 2284 2541 2768 2972 3061 3575
Size: 399 426 68 257 227 204 89 514
Cuts at: 3575 4317 4402 5473 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 514 426 399 257 227
204 89 85 68
BspEI T'CCGG_A
Cuts at: 5919 5919
Size: 6256
BspHI T'CATG_A
Cuts at: 4145 5875 4145
Size: 1730 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2616 3611 844
Size: 1772 995 3489
Fragments arranged by size:
3489 1772 995
BsrBI CCG'CTC
Cuts at: 1394 1823 2061 2295 4143 1394
Size: 429 238 234 1848 3507
Fragments arranged by size:
3507 1848 429 238 234
BsrDI GCAATG_nn'
Cuts at: 65 1248 4751 4925 65
Size: 1183 3503 174 1396
Fragments arranged by size:
3503 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2038 2314 2380 2837 4900 5087 1700
Size: 110 228 276 66 457 2063 187 2869
Fragments arranged by size:
2869 2063 457 276 228 187 110 66
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6256
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6256
BssSI C'ACGA_G
Cuts at: 2459 4316 5328 2459
Size: 1857 1012 3387
Fragments arranged by size:
3387 1857 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6256
BstBI TT'CG_AA
Cuts at: 5060 5060
Size: 6256
BstEII G'GTnAC_C
Cuts at: 1184 2662 1184
Size: 1478 4778
BstXI CCAn_nnnn'nTGG
Cuts at: 2510 2510
Size: 6256
BstYI r'GATC_y
Cuts at: 1972 2746 3586 3953 4338 4355 5807 5893 5905
Size: 774 840 367 385 17 1452 86 12
Cuts at: 5905 6251 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 840 774 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2179 2751 513
Size: 552 1114 572 4018
Fragments arranged by size:
4018 1114 572 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5517
Fragments arranged by size:
5517 683 56
BtsI GCAGTG_nn'
Cuts at: 2312 2474 2881 3772 4566 4586 2312
Size: 162 407 891 794 20 3982
Fragments arranged by size:
3982 891 794 407 162 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6221
DraI TTT'AAA
Cuts at: 3560 3560
Size: 6256
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2500 2575 3461
Size: 54 1425 50 275 18 670 75 886
Cuts at: 3461 3703 4592 6221 8
Size: 242 889 1629 43
Fragments arranged by size:
1629 1425 889 886 670 275 242 75
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3703 1537
Size: 275 1891 4090
Fragments arranged by size:
4090 1891 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4186 1353
Size: 2833 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2376 2544 3494 4843 5217 5363
Size: 1064 138 787 168 950 1349 374 146
Cuts at: 5363 387
Size: 1280
Fragments arranged by size:
1349 1280 1064 950 787 374 168 146
138
EcoICRI GAG'CTC
Cuts at: 2970 2970
Size: 6256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2956 3073 3141 3422 3433 3527
Size: 393 515 809 117 68 281 11 94
Cuts at: 3527 4319 4916 5703 5971 1239
Size: 792 597 787 268 1524
Fragments arranged by size:
1524 809 792 787 597 515 393 281
268 117 94 68 11
EcoO109I rG'GnC_Cy
Cuts at: 2011 2107 2227 2538 2011
Size: 96 120 311 5729
Fragments arranged by size:
5729 311 120 96
EcoRI G'AATT_C
Cuts at: 3565 3943 3565
Size: 378 5878
FspI TGC'GCA
Cuts at: 4762 4762
Size: 6256
HaeII r_GCGC'y
Cuts at: 1678 2139 2154 2315 2343 2481 3554 5403 1678
Size: 461 15 161 28 138 1073 1849 2531
Fragments arranged by size:
2531 1849 1073 461 161 138 28 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2908 2940 4897
Size: 32 135 32 455 32 849 32 1957
Cuts at: 4897 4929 4971 5003 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1957 849 455 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3600 3846 669
Size: 988 281 1662 246 3079
Fragments arranged by size:
3079 1662 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6256
HpaI GTT'AAC
Cuts at: 3846 3846
Size: 6256
KasI G'GCGC_C
Cuts at: 2135 2311 2135
Size: 176 6080
KpnI G_GTAC'C
Cuts at: 3581 3581
Size: 6256
MfeI C'AATT_G
Cuts at: 3855 3855
Size: 6256
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2241 3417 5370 5554 1305
Size: 936 1176 1953 184 2007
Fragments arranged by size:
2007 1953 1176 936 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3463 10
Size: 54 1425 343 1631 2803
Fragments arranged by size:
2803 1631 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3252 3268 3342 3358 4214 4573 4732
Size: 648 2086 16 74 16 856 359 159
Cuts at: 4732 518
Size: 2042
Fragments arranged by size:
2086 2042 856 648 359 159 74 16
16
MspA1I CmG'CkG
Cuts at: 1606 2181 2616 2832 3453 3547 3672 4349 5497
Size: 575 435 216 621 94 125 677 1148
Cuts at: 5497 5742 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 621 575 435 245 216
125 94
NaeI GCC'GGC
Cuts at: 2040 2040
Size: 6256
NarI GG'CG_CC
Cuts at: 2136 2312 2136
Size: 176 6080
NcoI C'CATG_G
Cuts at: 513 1065 2751 513
Size: 552 1686 4018
Fragments arranged by size:
4018 1686 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6256
NgoMIV G'CCGG_C
Cuts at: 2038 2038
Size: 6256
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6256
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NsiI A_TGCA'T
Cuts at: 2915 3522 2915
Size: 607 5649
NspI r_CATG'y
Cuts at: 1132 1229 3520 3614 5159 1132
Size: 97 2291 94 1545 2229
Fragments arranged by size:
2291 2229 1545 97 94
PasI CC'CwG_GG
Cuts at: 1348 2260 1348
Size: 912 5344
PciI A'CATG_T
Cuts at: 5155 5155
Size: 6256
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6256
PfoI T'CCnGG_A
Cuts at: 2031 2103 2031
Size: 72 6184
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PmlI CAC'GTG
Cuts at: 2025 2025
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 5838 5870 4325
Size: 32 1481 32 4711
Fragments arranged by size:
4711 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2011 2107 2227 2011
Size: 96 120 6040
Fragments arranged by size:
6040 120 96
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 3826 3826
Size: 6256
PspOMI G'GGCC_C
Cuts at: 2537 2537
Size: 6256
PstI C_TGCA'G
Cuts at: 830 3608 830
Size: 2778 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
PvuII CAG'CTG
Cuts at: 2616 2832 3453 3547 2616
Size: 216 621 94 5325
Fragments arranged by size:
5325 621 216 94
RsrII CG'GwC_CG
Cuts at: 3390 3390
Size: 6256
SacI G_AGCT'C
Cuts at: 2972 2972
Size: 6256
SacII CC_GC'GG
Cuts at: 2182 2182
Size: 6256
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SanDI GG'GwC_CC
Cuts at: 2107 2107
Size: 6256
SbfI CC_TGCA'GG
Cuts at: 3608 3608
Size: 6256
ScaI AGT'ACT
Cuts at: 2786 4504 6216 2786
Size: 1718 1712 2826
Fragments arranged by size:
2826 1718 1712
SfcI C'TryA_G
Cuts at: 826 1045 2078 2869 3604 4739 5420 5611 826
Size: 219 1033 791 735 1135 681 191 1471
Fragments arranged by size:
1471 1135 1033 791 735 681 219 191
SfoI GGC'GCC
Cuts at: 2137 2313 2137
Size: 176 6080
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 2314 1700
Size: 614 5642
SmaI CCC'GGG
Cuts at: 1872 2112 2337 3583 1872
Size: 240 225 1246 4545
Fragments arranged by size:
4545 1246 240 225
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2621 4360 5261 5523 820
Size: 197 926 678 1739 901 262 1553
Fragments arranged by size:
1739 1553 926 901 678 262 197
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6256
SphI G_CATG'C
Cuts at: 1132 3614 1132
Size: 2482 3774
SspI AAT'ATT
Cuts at: 5 52 4180 5
Size: 47 4128 2081
Fragments arranged by size:
4128 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6256
StyI C'CwwG_G
Cuts at: 513 1065 2751 3695 513
Size: 552 1686 944 3074
Fragments arranged by size:
3074 1686 944 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2061 2252 2534 2762 4283 4300 4453
Size: 1255 481 191 282 228 1521 17 153
Cuts at: 4453 4638 5083 325
Size: 185 445 1498
Fragments arranged by size:
1521 1498 1255 481 445 282 228 191
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2784 4502 6214
Size: 274 80 33 51 1443 807 1718 1712
Cuts at: 6214 96
Size: 138
Fragments arranged by size:
1718 1712 1443 807 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6216 6248 6216
Size: 32 6224
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6256
XbaI T'CTAG_A
Cuts at: 3592 3592
Size: 6256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3544 3544
Size: 6256
XhoI C'TCGA_G
Cuts at: 1943 2621 1943
Size: 678 5578
XmaI C'CCGG_G
Cuts at: 1870 2110 2335 3581 1870
Size: 240 225 1246 4545
Fragments arranged by size:
4545 1246 240 225
XmnI GAAnn'nnTTC
Cuts at: 3298 3353 4385 3298
Size: 55 1032 5169
Fragments arranged by size:
5169 1032 55
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BplI BsiWI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI
PacI PspXI PsrI SapI SexAI SfiI SrfI SwaI