Restriction map for
pFN21AE2192
(Circular) (Six-base) MAPSORT of: pFN21AE2192.seq Check: 217 from: 1 to: 6427
pFN21AE2192 6427 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3640 3640
Size: 6427
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4237 278
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3770 1937
Size: 1833 4594
Acc65I G'GTAC_C
Cuts at: 3748 3748
Size: 6427
AclI AA'CG_TT
Cuts at: 1563 4554 4927 1563
Size: 2991 373 3063
Fragments arranged by size:
3063 2991 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2372 2662 3090 3207 3429 3681 4490 5874
Size: 740 290 428 117 222 252 809 1384
Cuts at: 5874 6142 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 809 740 428 290 268 252
222 117
AflII C'TTAA_G
Cuts at: 820 1017 2411 820
Size: 197 1394 4836
Fragments arranged by size:
4836 1394 197
AflIII A'CryG_T
Cuts at: 1203 5326 6303 1203
Size: 4123 977 1327
Fragments arranged by size:
4123 1327 977
AgeI A'CCGG_T
Cuts at: 5258 5258
Size: 6427
AhdI GACnn_n'nnGTC
Cuts at: 5156 5156
Size: 6427
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6427
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2749 2781 2749
Size: 32 6395
AlwNI CAG_nnn'CTG
Cuts at: 2822 4170 5742 2822
Size: 1348 1572 3507
Fragments arranged by size:
3507 1572 1348
ApaLI G'TGCA_C
Cuts at: 3653 4484 5640 3653
Size: 831 1156 4440
Fragments arranged by size:
4440 1156 831
ApoI r'AATT_y
Cuts at: 1493 2968 3008 3624 3736 3962 4114 6223 1493
Size: 1475 40 616 112 226 152 2109 1697
Fragments arranged by size:
2109 1697 1475 616 226 152 112 40
AseI AT'TA_AT
Cuts at: 160 1032 4981 160
Size: 872 3949 1606
Fragments arranged by size:
3949 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
AvaI C'yCGr_G
Cuts at: 1870 1943 3752 1870
Size: 73 1809 4545
Fragments arranged by size:
4545 1809 73
BamHI G'GATC_C
Cuts at: 3757 4124 3757
Size: 367 6060
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3748 5203 618
Size: 325 217 627 1961 1455 1842
Fragments arranged by size:
1961 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3746 1391
Size: 2355 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2312 3669 928
Size: 971 413 1357 3686
Fragments arranged by size:
3686 1357 971 413
BbvCI CC'TCA_GC
Cuts at: 3450 3450
Size: 6427
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2535 2569 4618 4652 1903
Size: 34 598 34 2049 34 3678
Fragments arranged by size:
3678 2049 598 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3046 3450 3585 4319 5529 3046
Size: 404 135 734 1210 3944
Fragments arranged by size:
3944 1210 734 404 135
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6427
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5038 136
Size: 107 122 71 631 605 3366 1525
Fragments arranged by size:
3366 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6422 6422
Size: 6427
BlpI GC'TnA_GC
Cuts at: 3844 3844
Size: 6427
Bme1580I G_kGCm'C
Cuts at: 1790 2012 3657 4488 5644 1790
Size: 222 1645 831 1156 2573
Fragments arranged by size:
2573 1645 1156 831 222
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6427
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2025 4158 5116
Size: 416 53 83 399 24 608 2133 958
Cuts at: 5116 442
Size: 1753
Fragments arranged by size:
2133 1753 958 608 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6427
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2904 3452 3493 5087 1239
Size: 803 862 548 41 1594 2579
Fragments arranged by size:
2579 1594 862 803 548 41
Bpu10I CC'TnA_GC
Cuts at: 3450 3450
Size: 6427
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4552 5417 5715 4552
Size: 865 298 5264
Fragments arranged by size:
5264 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2891 3512 5090 882
Size: 617 1392 621 1578 2219
Fragments arranged by size:
2219 1578 1392 621 617
BsaAI yAC'GTr
Cuts at: 493 1204 4218 493
Size: 711 3014 2702
Fragments arranged by size:
3014 2702 711
BsaBI GATnn'nnATC
Cuts at: 6421 6421
Size: 6427
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4234 4616 5251 275
Size: 53 83 186 936 2701 382 635 1451
Fragments arranged by size:
2701 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4860 5258 5532 5679 6090 4860
Size: 398 274 147 411 5197
Fragments arranged by size:
5197 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6202
Fragments arranged by size:
6202 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3297 1186
Size: 2111 4316
BseYI C'CCAG_C
Cuts at: 5630 6126 5630
Size: 496 5931
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5854
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3877 4638 5666 1540
Size: 275 186 1876 761 1028 2301
Fragments arranged by size:
2301 1876 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3657 4488 4573 5644 3657
Size: 831 85 1071 4440
Fragments arranged by size:
4440 1071 831 85
BsmI GAATG_Cn'
Cuts at: 2252 3938 4031 2252
Size: 1686 93 4648
Fragments arranged by size:
4648 1686 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 3657 3746 4488 4573 5644 1391
Size: 399 222 1645 89 742 85 1071 2174
Fragments arranged by size:
2174 1645 1071 742 399 222 89 85
BspEI T'CCGG_A
Cuts at: 6090 6090
Size: 6427
BspHI T'CATG_A
Cuts at: 3346 3685 3706 4316 6046 3346
Size: 339 21 610 1730 3727
Fragments arranged by size:
3727 1730 610 339 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3640 3782 844
Size: 2796 142 3489
Fragments arranged by size:
3489 2796 142
BsrBI CCG'CTC
Cuts at: 1394 1823 4314 1394
Size: 429 2491 3507
Fragments arranged by size:
3507 2491 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4922 5096 65
Size: 1183 3674 174 1396
Fragments arranged by size:
3674 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5071 5258 1700
Size: 110 3261 187 2869
Fragments arranged by size:
3261 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6427
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6427
BssSI C'ACGA_G
Cuts at: 4487 5499 4487
Size: 1012 5415
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6427
BstBI TT'CG_AA
Cuts at: 5231 5231
Size: 6427
BstEII G'GTnAC_C
Cuts at: 1184 3312 1184
Size: 2128 4299
BstXI CCAn_nnnn'nTGG
Cuts at: 2271 2271
Size: 6427
BstYI r'GATC_y
Cuts at: 1972 2360 3757 4124 4509 4526 5978 6064 6076
Size: 388 1397 367 385 17 1452 86 12
Cuts at: 6076 6422 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1397 388 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2537 3394 2537
Size: 857 5570
BtgI C'CryG_G
Cuts at: 513 1065 2004 2044 3353 513
Size: 552 939 40 1309 3587
Fragments arranged by size:
3587 1309 939 552 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2194 3352 487
Size: 683 56 968 1158 3562
Fragments arranged by size:
3562 1158 968 683 56
BtsI GCAGTG_nn'
Cuts at: 2185 3572 3943 4737 4757 2185
Size: 1387 371 794 20 3855
Fragments arranged by size:
3855 1387 794 371 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6427
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6392
DraI TTT'AAA
Cuts at: 3132 3731 3132
Size: 599 5828
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5434 809
Size: 507 4118 1802
Fragments arranged by size:
4118 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3874 4763 6392
Size: 54 1425 50 275 18 2044 889 1629
Cuts at: 6392 8
Size: 43
Fragments arranged by size:
2044 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3874 1537
Size: 275 2062 4090
Fragments arranged by size:
4090 2062 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2753 4357 1353
Size: 1400 1604 3423
Fragments arranged by size:
3423 1604 1400
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5014 5388 5534 387
Size: 1064 138 3425 374 146 1280
Fragments arranged by size:
3425 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2372 2662 2904 3090 3207 3429
Size: 393 410 330 290 242 186 117 222
Cuts at: 3429 3452 3493 3681 4490 5087 5874 6142 1239
Size: 23 41 188 809 597 787 268 1524
Fragments arranged by size:
1524 809 787 597 410 393 330 290
268 242 222 188 186 117 41 23
EcoO109I rG'GnC_Cy
Cuts at: 2541 2541
Size: 6427
EcoRI G'AATT_C
Cuts at: 3736 4114 3736
Size: 378 6049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3281 3356 3281
Size: 75 6352
FspI TGC'GCA
Cuts at: 4933 4933
Size: 6427
HaeII r_GCGC'y
Cuts at: 1678 2080 5574 1678
Size: 402 3494 2531
Fragments arranged by size:
3494 2531 402
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2017 2049 2485 2517 3292
Size: 32 135 32 445 32 436 32 775
Cuts at: 3292 3324 5068 5100 5142 5174 1373
Size: 32 1744 32 42 32 2626
Fragments arranged by size:
2626 1744 775 445 436 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2674 3771 4017 669
Size: 988 281 736 1097 246 3079
Fragments arranged by size:
3079 1097 988 736 281 246
HindIII A'AGCT_T
Cuts at: 748 3359 748
Size: 2611 3816
HpaI GTT'AAC
Cuts at: 2674 4017 2674
Size: 1343 5084
KpnI G_GTAC'C
Cuts at: 3752 3752
Size: 6427
MfeI C'AATT_G
Cuts at: 3579 4026 3579
Size: 447 5980
MluI A'CGCG_T
Cuts at: 6303 6303
Size: 6427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5541 5725 1305
Size: 4236 184 2007
Fragments arranged by size:
4236 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4605
Fragments arranged by size:
4605 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2269 2394 4385 4744 4903 518
Size: 648 1103 125 1991 359 159 2042
Fragments arranged by size:
2042 1991 1103 648 359 159 125
MspA1I CmG'CkG
Cuts at: 1606 2046 3449 3843 4520 5668 5913 1606
Size: 440 1403 394 677 1148 245 2120
Fragments arranged by size:
2120 1403 1148 677 440 394 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3353 513
Size: 552 939 1349 3587
Fragments arranged by size:
3587 1349 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6427
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6427
NotI GC'GGCC_GC
Cuts at: 3874 3874
Size: 6427
NspI r_CATG'y
Cuts at: 1132 1229 2892 3785 5330 1132
Size: 97 1663 893 1545 2229
Fragments arranged by size:
2229 1663 1545 893 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6427
PciI A'CATG_T
Cuts at: 5326 5326
Size: 6427
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6427
PmeI GTTT'AAAC
Cuts at: 3731 3731
Size: 6427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 6009 6041 4496
Size: 32 1481 32 4882
Fragments arranged by size:
4882 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2541 2541
Size: 6427
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6427
PsiI TTA'TAA
Cuts at: 3997 3997
Size: 6427
PstI C_TGCA'G
Cuts at: 830 2239 2480 3779 830
Size: 1409 241 1299 3478
Fragments arranged by size:
3478 1409 1299 241
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6427
PvuII CAG'CTG
Cuts at: 3449 3449
Size: 6427
SacII CC_GC'GG
Cuts at: 2047 2047
Size: 6427
SalI G'TCGA_C
Cuts at: 1936 3769 1936
Size: 1833 4594
SapI GCTCTTCn'nnn_
Cuts at: 2753 2753
Size: 6427
SbfI CC_TGCA'GG
Cuts at: 3779 3779
Size: 6427
ScaI AGT'ACT
Cuts at: 4675 6387 4675
Size: 1712 4715
SfcI C'TryA_G
Cuts at: 826 1045 2235 2476 3775 4910 5591 5782 826
Size: 219 1190 241 1299 1135 681 191 1471
Fragments arranged by size:
1471 1299 1190 1135 681 241 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6427
SmaI CCC'GGG
Cuts at: 1872 3754 1872
Size: 1882 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2411 4531 5432 5694 820
Size: 197 926 468 2120 901 262 1553
Fragments arranged by size:
2120 1553 926 901 468 262 197
SnaBI TAC'GTA
Cuts at: 493 4218 493
Size: 3725 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6427
SphI G_CATG'C
Cuts at: 1132 3785 1132
Size: 2653 3774
SspI AAT'ATT
Cuts at: 5 52 4351 5
Size: 47 4299 2081
Fragments arranged by size:
4299 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6427
StyI C'CwwG_G
Cuts at: 513 1065 2004 3353 3866 513
Size: 552 939 1349 513 3074
Fragments arranged by size:
3074 1349 939 552 513
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3300 4454 4471 4624 4809 5254 325
Size: 1255 1720 1154 17 153 185 445 1498
Fragments arranged by size:
1720 1498 1255 1154 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2328 2437 4673
Size: 274 80 33 51 1443 351 109 2236
Cuts at: 4673 6385 96
Size: 1712 138
Fragments arranged by size:
2236 1712 1443 351 274 138 109 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6387 6419 6387
Size: 32 6395
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6427
XbaI T'CTAG_A
Cuts at: 3763 3763
Size: 6427
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6427
XmaI C'CCGG_G
Cuts at: 1870 3752 1870
Size: 1882 4545
XmnI GAAnn'nnTTC
Cuts at: 3179 4556 3179
Size: 1377 5050
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4235 276
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SanDI
SexAI SfiI SfoI SrfI SwaI XcmI