Restriction map for
pFN21AE2185
(Circular) (Six-base) MAPSORT of: pFN21AE2185.seq Check: 4867 from: 1 to: 5551
pFN21AE2185 5551 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3361 278
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2894 1937
Size: 957 4594
Acc65I G'GTAC_C
Cuts at: 2872 2872
Size: 5551
AclI AA'CG_TT
Cuts at: 1563 3678 4051 1563
Size: 2115 373 3063
Fragments arranged by size:
3063 2115 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2240 2437 3614 4998 5266 1632
Size: 608 197 1177 1384 268 1917
Fragments arranged by size:
1917 1384 1177 608 268 197
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5354
AflIII A'CryG_T
Cuts at: 1203 4450 5427 1203
Size: 3247 977 1327
Fragments arranged by size:
3247 1327 977
AgeI A'CCGG_T
Cuts at: 4382 4382
Size: 5551
AhdI GACnn_n'nnGTC
Cuts at: 4280 4280
Size: 5551
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5551
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2276 2308 2276
Size: 32 5519
AlwNI CAG_nnn'CTG
Cuts at: 2378 3294 4866 2378
Size: 916 1572 3063
Fragments arranged by size:
3063 1572 916
ApaLI G'TGCA_C
Cuts at: 3608 4764 3608
Size: 1156 4395
ApoI r'AATT_y
Cuts at: 1493 2860 3086 3238 5347 1493
Size: 1367 226 152 2109 1697
Fragments arranged by size:
2109 1697 1367 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4105 160
Size: 872 3073 1606
Fragments arranged by size:
3073 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
AvaI C'yCGr_G
Cuts at: 1870 1943 2876 1870
Size: 73 933 4545
Fragments arranged by size:
4545 933 73
BamHI G'GATC_C
Cuts at: 2881 3248 2881
Size: 367 5184
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2381 2872 4327 618
Size: 325 217 627 594 491 1455 1842
Fragments arranged by size:
1842 1455 627 594 491 325 217
BanII G_rGCy'C
Cuts at: 1391 2870 1391
Size: 1479 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4580
BbvCI CC'TCA_GC
Cuts at: 2134 2134
Size: 5551
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3742 3776 1903
Size: 34 1805 34 3678
Fragments arranged by size:
3678 1805 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3443 4653 3443
Size: 1210 4341
BclI T'GATC_A
Cuts at: 1548 2320 1548
Size: 772 4779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4162 136
Size: 107 122 71 631 605 2490 1525
Fragments arranged by size:
2490 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5546 5546
Size: 5551
BlpI GC'TnA_GC
Cuts at: 2968 2968
Size: 5551
Bme1580I G_kGCm'C
Cuts at: 1790 2818 3612 4768 1790
Size: 1028 794 1156 2573
Fragments arranged by size:
2573 1156 1028 794
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5551
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3282 4240 442
Size: 416 53 83 399 24 1865 958 1753
Fragments arranged by size:
1865 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5551
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 5551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2053 2085 2466 4211 1239
Size: 814 32 381 1745 2579
Fragments arranged by size:
2579 1745 814 381 32
Bpu10I CC'TnA_GC
Cuts at: 2134 2165 2134
Size: 31 5520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2453 3676 4541 4839 2453
Size: 1223 865 298 3165
Fragments arranged by size:
3165 1223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2489 4214 882
Size: 617 990 1725 2219
Fragments arranged by size:
2219 1725 990 617
BsaAI yAC'GTr
Cuts at: 493 1204 3342 493
Size: 711 2138 2702
Fragments arranged by size:
2702 2138 711
BsaBI GATnn'nnATC
Cuts at: 5545 5545
Size: 5551
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3358 3740 4375 275
Size: 53 83 186 936 1825 382 635 1451
Fragments arranged by size:
1825 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3984 4382 4656 4803 5214 3984
Size: 398 274 147 411 4321
Fragments arranged by size:
4321 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2657 2687 1178
Size: 30 165 30 1254 30 4042
Fragments arranged by size:
4042 1254 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5551
BseYI C'CCAG_C
Cuts at: 2138 2529 2811 4754 5250 2138
Size: 391 282 1943 496 2439
Fragments arranged by size:
2439 1943 496 391 282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2155 1163
Size: 573 419 4559
Fragments arranged by size:
4559 573 419
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3001 3762 4790 1540
Size: 275 186 1000 761 1028 2301
Fragments arranged by size:
2301 1028 1000 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2646 3612 3697 4768 2646
Size: 966 85 1071 3429
Fragments arranged by size:
3429 1071 966 85
BsmI GAATG_Cn'
Cuts at: 2428 3062 3155 2428
Size: 634 93 4824
Fragments arranged by size:
4824 634 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2646 2818 2870 3612 3697 4768 1391
Size: 399 856 172 52 742 85 1071 2174
Fragments arranged by size:
2174 1071 856 742 399 172 85 52
BspEI T'CCGG_A
Cuts at: 5214 5214
Size: 5551
BspHI T'CATG_A
Cuts at: 3440 5170 3440
Size: 1730 3821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2906 844
Size: 2062 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3438 1394
Size: 429 1615 3507
Fragments arranged by size:
3507 1615 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2344 2694 4046 4220 65
Size: 1183 1096 350 1352 174 1396
Fragments arranged by size:
1396 1352 1183 1096 350 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4195 4382 1700
Size: 110 2385 187 2869
Fragments arranged by size:
2869 2385 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5551
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5551
BssSI C'ACGA_G
Cuts at: 3611 4623 3611
Size: 1012 4539
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2315 794
Size: 1521 4030
BstBI TT'CG_AA
Cuts at: 4355 4355
Size: 5551
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5551
BstYI r'GATC_y
Cuts at: 1972 2022 2881 3248 3633 3650 5102 5188 5200
Size: 50 859 367 385 17 1452 86 12
Cuts at: 5200 5546 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 859 385 367 346 86 50
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4060
Fragments arranged by size:
4060 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4812
Fragments arranged by size:
4812 683 56
BtsI GCAGTG_nn'
Cuts at: 2316 3067 3861 3881 2316
Size: 751 794 20 3986
Fragments arranged by size:
3986 794 751 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5551
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5516
DraI TTT'AAA
Cuts at: 2855 2855
Size: 5551
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4558 809
Size: 507 3242 1802
Fragments arranged by size:
3242 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2998 3887 5516
Size: 54 1425 50 275 18 1168 889 1629
Cuts at: 5516 8
Size: 43
Fragments arranged by size:
1629 1425 1168 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2998 1537
Size: 275 1186 4090
Fragments arranged by size:
4090 1186 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2033 2797 3481 1353
Size: 680 764 684 3423
Fragments arranged by size:
3423 764 684 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2761 4138 4512 4658 387
Size: 1064 138 1172 1377 374 146 1280
Fragments arranged by size:
1377 1280 1172 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2085 2240 2437 2466 3614 4211
Size: 393 421 32 155 197 29 1148 597
Cuts at: 4211 4998 5266 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1148 787 597 421 393 268 197
155 32 29
EcoRI G'AATT_C
Cuts at: 2860 3238 2860
Size: 378 5173
EcoRV GAT'ATC
Cuts at: 2203 2716 2203
Size: 513 5038
FspI TGC'GCA
Cuts at: 4057 4057
Size: 5551
HaeII r_GCGC'y
Cuts at: 1678 4698 1678
Size: 3020 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2097 2129 2258 2290 4192
Size: 32 135 32 525 32 129 32 1902
Cuts at: 4192 4224 4266 4298 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1902 525 135 129 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2560 2895 3141 669
Size: 988 281 622 335 246 3079
Fragments arranged by size:
3079 988 622 335 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5551
HpaI GTT'AAC
Cuts at: 3141 3141
Size: 5551
KpnI G_GTAC'C
Cuts at: 2876 2876
Size: 5551
MfeI C'AATT_G
Cuts at: 2070 3150 2070
Size: 1080 4471
MluI A'CGCG_T
Cuts at: 5427 5427
Size: 5551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2256 4665 4849 1305
Size: 951 2409 184 2007
Fragments arranged by size:
2409 2007 951 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3729
Fragments arranged by size:
3729 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3509 3868 4027 518
Size: 648 2343 359 159 2042
Fragments arranged by size:
2343 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2138 2761 2811 2967 3644 4792 5037 1606
Size: 532 623 50 156 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 623 532 245 156 50
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4060
Fragments arranged by size:
4060 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5551
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5551
NotI GC'GGCC_GC
Cuts at: 2998 2998
Size: 5551
NspI r_CATG'y
Cuts at: 1132 1229 2461 2909 4454 1132
Size: 97 1232 448 1545 2229
Fragments arranged by size:
2229 1545 1232 448 97
PasI CC'CwG_GG
Cuts at: 1348 2014 1348
Size: 666 4885
PciI A'CATG_T
Cuts at: 4450 4450
Size: 5551
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2811 1108
Size: 1703 3848
PmeI GTTT'AAAC
Cuts at: 2855 2855
Size: 5551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3620 3652 5133 5165 3620
Size: 32 1481 32 4006
Fragments arranged by size:
4006 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5551
PsiI TTA'TAA
Cuts at: 3121 3121
Size: 5551
PstI C_TGCA'G
Cuts at: 830 2903 830
Size: 2073 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5551
PvuII CAG'CTG
Cuts at: 2138 2761 2811 2138
Size: 623 50 4878
Fragments arranged by size:
4878 623 50
SalI G'TCGA_C
Cuts at: 1936 2893 1936
Size: 957 4594
SbfI CC_TGCA'GG
Cuts at: 2903 2903
Size: 5551
ScaI AGT'ACT
Cuts at: 3799 5511 3799
Size: 1712 3839
SfcI C'TryA_G
Cuts at: 826 1045 2756 2899 4034 4715 4906 826
Size: 219 1711 143 1135 681 191 1471
Fragments arranged by size:
1711 1471 1135 681 219 191 143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5551
SmaI CCC'GGG
Cuts at: 1872 2878 1872
Size: 1006 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2432 3655 4556 4818 820
Size: 197 926 489 1223 901 262 1553
Fragments arranged by size:
1553 1223 926 901 489 262 197
SnaBI TAC'GTA
Cuts at: 493 3342 493
Size: 2849 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5551
SphI G_CATG'C
Cuts at: 1132 2461 2909 1132
Size: 1329 448 3774
Fragments arranged by size:
3774 1329 448
SspI AAT'ATT
Cuts at: 5 52 3475 5
Size: 47 3423 2081
Fragments arranged by size:
3423 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5551
StyI C'CwwG_G
Cuts at: 513 1065 2004 2990 513
Size: 552 939 986 3074
Fragments arranged by size:
3074 986 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3578 3595 3748 3933 4378 325
Size: 1255 1998 17 153 185 445 1498
Fragments arranged by size:
1998 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3797 5509 96
Size: 274 80 33 51 1443 1820 1712 138
Fragments arranged by size:
1820 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5511 5543 5511
Size: 32 5519
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5551
XbaI T'CTAG_A
Cuts at: 2887 2887
Size: 5551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5551
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5551
XmaI C'CCGG_G
Cuts at: 1870 2876 1870
Size: 1006 4545
XmnI GAAnn'nnTTC
Cuts at: 3680 3680
Size: 5551
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3359 276
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI