Restriction map for
pFN21AE2166
(Circular) (Six-base) MAPSORT of: pFN21AE2166.seq Check: 7417 from: 1 to: 5764
pFN21AE2166 5764 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3574 278
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3107 1937
Size: 1170 4594
Acc65I G'GTAC_C
Cuts at: 3085 3085
Size: 5764
AclI AA'CG_TT
Cuts at: 1563 3891 4264 1563
Size: 2328 373 3063
Fragments arranged by size:
3063 2328 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2241 3827 5211 5479 1632
Size: 609 1586 1384 268 1917
Fragments arranged by size:
1917 1586 1384 609 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5567
AflIII A'CryG_T
Cuts at: 1203 2949 4663 5640 1203
Size: 1746 1714 977 1327
Fragments arranged by size:
1746 1714 1327 977
AgeI A'CCGG_T
Cuts at: 4595 4595
Size: 5764
AhdI GACnn_n'nnGTC
Cuts at: 4493 4493
Size: 5764
AleI CACnn'nnGTG
Cuts at: 1166 2551 1166
Size: 1385 4379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5764
AlwNI CAG_nnn'CTG
Cuts at: 3507 5079 3507
Size: 1572 4192
ApaI G_GGCC'C
Cuts at: 2756 2756
Size: 5764
ApaLI G'TGCA_C
Cuts at: 3821 4977 3821
Size: 1156 4608
ApoI r'AATT_y
Cuts at: 1493 3073 3299 3451 5560 1493
Size: 1580 226 152 2109 1697
Fragments arranged by size:
2109 1697 1580 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4318 160
Size: 872 3286 1606
Fragments arranged by size:
3286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
AvaI C'yCGr_G
Cuts at: 1870 1943 2906 3089 1870
Size: 73 963 183 4545
Fragments arranged by size:
4545 963 183 73
BamHI G'GATC_C
Cuts at: 3094 3461 3094
Size: 367 5397
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2382 2630 3085 4540
Size: 325 217 627 258 337 248 455 1455
Cuts at: 4540 618
Size: 1842
Fragments arranged by size:
1842 1455 627 455 337 325 258 248
217
BanII G_rGCy'C
Cuts at: 1391 2142 2508 2756 3083 1391
Size: 751 366 248 327 4072
Fragments arranged by size:
4072 751 366 327 248
BbeI G_GCGC'C
Cuts at: 2049 2049
Size: 5764
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2767 928
Size: 971 868 3925
Fragments arranged by size:
3925 971 868
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2962 2996 3955 3989 1903
Size: 34 1025 34 959 34 3678
Fragments arranged by size:
3678 1025 959 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3656 4866 3656
Size: 1210 4554
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5764
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4375 136
Size: 107 122 71 631 605 2703 1525
Fragments arranged by size:
2703 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5759 5759
Size: 5764
BlpI GC'TnA_GC
Cuts at: 3181 3181
Size: 5764
Bme1580I G_kGCm'C
Cuts at: 1790 2633 2756 2975 3001 3825 4981 1790
Size: 843 123 219 26 824 1156 2573
Fragments arranged by size:
2573 1156 843 824 219 123 26
BmgBI CAC'GTC
Cuts at: 1318 2991 1318
Size: 1673 4091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2629 3495 4453
Size: 416 53 83 399 24 1212 866 958
Cuts at: 4453 442
Size: 1753
Fragments arranged by size:
1753 1212 958 866 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2214 2270 2521 4424 1239
Size: 975 56 251 1903 2579
Fragments arranged by size:
2579 1903 975 251 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2582 3889 4754 5052 2582
Size: 1307 865 298 3294
Fragments arranged by size:
3294 1307 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4427 882
Size: 617 2928 2219
Fragments arranged by size:
2928 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2332 2950 3555 493
Size: 711 1128 618 605 2702
Fragments arranged by size:
2702 1128 711 618 605
BsaBI GATnn'nnATC
Cuts at: 5758 5758
Size: 5764
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 2532 2829 3571
Size: 53 83 186 936 513 486 297 742
Cuts at: 3571 3953 4588 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 742 635 513 486 382 297
186 83 53
BsaWI w'CCGG_w
Cuts at: 2262 4197 4595 4869 5016 5427 2262
Size: 1935 398 274 147 411 2599
Fragments arranged by size:
2599 1935 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5539
Fragments arranged by size:
5539 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2924 1186
Size: 1738 4026
BseYI C'CCAG_C
Cuts at: 2499 2626 3009 4967 5463 2499
Size: 127 383 1958 496 2800
Fragments arranged by size:
2800 1958 496 383 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2050 2510 1163
Size: 573 314 460 4417
Fragments arranged by size:
4417 573 460 314
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2307 2487 3214 3975 5003 1540
Size: 275 186 306 180 727 761 1028 2301
Fragments arranged by size:
2301 1028 761 727 306 275 186 180
BsiHKAI G_wGCw'C
Cuts at: 2294 2526 3825 3910 4981 2294
Size: 232 1299 85 1071 3077
Fragments arranged by size:
3077 1299 1071 232 85
BsmI GAATG_Cn'
Cuts at: 3275 3368 3275
Size: 93 5671
BsmBI CGTCTCn'nnnn_
Cuts at: 2110 2110
Size: 5764
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2294 2508 2526 2633 2756 2975
Size: 399 352 152 214 18 107 123 219
Cuts at: 2975 3001 3083 3825 3910 4981 1391
Size: 26 82 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 352 219 214 152
123 107 85 82 26 18
BspEI T'CCGG_A
Cuts at: 5427 5427
Size: 5764
BspHI T'CATG_A
Cuts at: 2112 3653 5383 2112
Size: 1541 1730 2493
Fragments arranged by size:
2493 1730 1541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2175 2448 2520 2613 3119 844
Size: 1331 273 72 93 506 3489
Fragments arranged by size:
3489 1331 506 273 93 72
BsrBI CCG'CTC
Cuts at: 1394 1823 3651 1394
Size: 429 1828 3507
Fragments arranged by size:
3507 1828 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4259 4433 65
Size: 1183 3011 174 1396
Fragments arranged by size:
3011 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2679 4408 4595 1700
Size: 110 869 1729 187 2869
Fragments arranged by size:
2869 1729 869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5764
BssSI C'ACGA_G
Cuts at: 2684 3041 3824 4836 2684
Size: 357 783 1012 3612
Fragments arranged by size:
3612 1012 783 357
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5764
BstBI TT'CG_AA
Cuts at: 4568 4568
Size: 5764
BstEII G'GTnAC_C
Cuts at: 1184 2744 1184
Size: 1560 4204
BstXI CCAn_nnnn'nTGG
Cuts at: 2626 2626
Size: 5764
BstYI r'GATC_y
Cuts at: 1972 2178 2736 3094 3461 3846 3863 5315 5401
Size: 206 558 358 367 385 17 1452 86
Cuts at: 5401 5413 5759 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 558 385 367 358 346 206
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2847 2847
Size: 5764
BtgI C'CryG_G
Cuts at: 513 1065 2218 2824 2918 513
Size: 552 1153 606 94 3359
Fragments arranged by size:
3359 1153 606 552 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5025
Fragments arranged by size:
5025 683 56
BtsI GCAGTG_nn'
Cuts at: 2999 3280 4074 4094 2999
Size: 281 794 20 4669
Fragments arranged by size:
4669 794 281 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5729
DraI TTT'AAA
Cuts at: 3068 3068
Size: 5764
DraIII CAC_nnn'GTG
Cuts at: 2131 2131
Size: 5764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4771 809
Size: 507 3455 1802
Fragments arranged by size:
3455 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2304 2469 2484
Size: 54 1425 50 275 18 474 165 15
Cuts at: 2484 2805 3211 4100 5729 8
Size: 321 406 889 1629 43
Fragments arranged by size:
1629 1425 889 474 406 321 275 165
54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2304 2484 3211 1537
Size: 275 492 180 727 4090
Fragments arranged by size:
4090 727 492 275 180
EarI CTCTTCn'nnn_
Cuts at: 1353 2957 3694 1353
Size: 1604 737 3423
Fragments arranged by size:
3423 1604 737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4351 4725 4871 387
Size: 1064 138 2762 374 146 1280
Fragments arranged by size:
2762 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2214 2241 2270 2521 3827 4424 5211
Size: 393 582 27 29 251 1306 597 787
Cuts at: 5211 5479 1239
Size: 268 1524
Fragments arranged by size:
1524 1306 787 597 582 393 268 251
29 27
EcoO109I rG'GnC_Cy
Cuts at: 2752 2752
Size: 5764
EcoRI G'AATT_C
Cuts at: 3073 3451 3073
Size: 378 5386
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2905 2905
Size: 5764
FspI TGC'GCA
Cuts at: 4270 4270
Size: 5764
HaeII r_GCGC'y
Cuts at: 1678 2049 2500 2680 2699 4911 1678
Size: 371 451 180 19 2212 2531
Fragments arranged by size:
2531 2212 451 371 180 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2728 2760 2975 3007 4405
Size: 32 135 32 1156 32 215 32 1398
Cuts at: 4405 4437 4479 4511 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1398 1156 215 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3108 3354 669
Size: 988 281 1170 246 3079
Fragments arranged by size:
3079 1170 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5764
HpaI GTT'AAC
Cuts at: 3354 3354
Size: 5764
KasI G'GCGC_C
Cuts at: 2045 2045
Size: 5764
KpnI G_GTAC'C
Cuts at: 3089 3089
Size: 5764
MfeI C'AATT_G
Cuts at: 3363 3363
Size: 5764
MluI A'CGCG_T
Cuts at: 5640 5640
Size: 5764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2553 4878 5062 1305
Size: 1248 2325 184 2007
Fragments arranged by size:
2325 2007 1248 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3942
Fragments arranged by size:
3942 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2551 3053 3722 4081 4240 518
Size: 648 1385 502 669 359 159 2042
Fragments arranged by size:
2042 1385 669 648 502 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 2434 2761 2826 2920 3013 3180 3857
Size: 453 375 327 65 94 93 167 677
Cuts at: 3857 5005 5250 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 453 375 327 245 167
94 93 65
NaeI GCC'GGC
Cuts at: 2681 2681
Size: 5764
NarI GG'CG_CC
Cuts at: 2046 2046
Size: 5764
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5212
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5764
NgoMIV G'CCGG_C
Cuts at: 2679 2679
Size: 5764
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5764
NotI GC'GGCC_GC
Cuts at: 3211 3211
Size: 5764
NspI r_CATG'y
Cuts at: 1132 1229 2707 3020 3122 4667 1132
Size: 97 1478 313 102 1545 2229
Fragments arranged by size:
2229 1545 1478 313 102 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5764
PciI A'CATG_T
Cuts at: 4663 4663
Size: 5764
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2335 2868 1108
Size: 1227 533 4004
Fragments arranged by size:
4004 1227 533
PfoI T'CCnGG_A
Cuts at: 2247 2454 2537 2247
Size: 207 83 5474
Fragments arranged by size:
5474 207 83
PmeI GTTT'AAAC
Cuts at: 3068 3068
Size: 5764
PmlI CAC'GTG
Cuts at: 2332 2950 2332
Size: 618 5146
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 5346 5378 3833
Size: 32 1481 32 4219
Fragments arranged by size:
4219 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5764
PsiI TTA'TAA
Cuts at: 3334 3334
Size: 5764
PspOMI G'GGCC_C
Cuts at: 2752 2752
Size: 5764
PstI C_TGCA'G
Cuts at: 830 3116 830
Size: 2286 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5764
PvuII CAG'CTG
Cuts at: 2761 3013 2761
Size: 252 5512
SacII CC_GC'GG
Cuts at: 2827 2921 2827
Size: 94 5670
SalI G'TCGA_C
Cuts at: 1936 3106 1936
Size: 1170 4594
SapI GCTCTTCn'nnn_
Cuts at: 2957 2957
Size: 5764
SbfI CC_TGCA'GG
Cuts at: 3116 3116
Size: 5764
ScaI AGT'ACT
Cuts at: 2888 4012 5724 2888
Size: 1124 1712 2928
Fragments arranged by size:
2928 1712 1124
SfcI C'TryA_G
Cuts at: 826 1045 3112 4247 4928 5119 826
Size: 219 2067 1135 681 191 1471
Fragments arranged by size:
2067 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2047 2047
Size: 5764
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5764
SmaI CCC'GGG
Cuts at: 1872 3091 1872
Size: 1219 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2597 3868 4769 5031 820
Size: 197 926 654 1271 901 262 1553
Fragments arranged by size:
1553 1271 926 901 654 262 197
SnaBI TAC'GTA
Cuts at: 493 3555 493
Size: 3062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5764
SphI G_CATG'C
Cuts at: 1132 2707 3020 3122 1132
Size: 1575 313 102 3774
Fragments arranged by size:
3774 1575 313 102
SspI AAT'ATT
Cuts at: 5 52 3688 5
Size: 47 3636 2081
Fragments arranged by size:
3636 2081 47
StuI AGG'CCT
Cuts at: 1517 2852 1517
Size: 1335 4429
StyI C'CwwG_G
Cuts at: 513 1065 2051 2142 3203 513
Size: 552 986 91 1061 3074
Fragments arranged by size:
3074 1061 986 552 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2648 3791 3808 3961 4146 4591 325
Size: 1255 1068 1143 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1143 1068 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2886 3057 4010
Size: 274 80 33 51 1443 909 171 953
Cuts at: 4010 5722 96
Size: 1712 138
Fragments arranged by size:
1712 1443 953 909 274 171 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2309 2341 5724 5756 2309
Size: 32 3383 32 2317
Fragments arranged by size:
3383 2317 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5764
XbaI T'CTAG_A
Cuts at: 3100 3100
Size: 5764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2800 3001 2800
Size: 201 5563
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5764
XmaI C'CCGG_G
Cuts at: 1870 3089 1870
Size: 1219 4545
XmnI GAAnn'nnTTC
Cuts at: 3893 3893
Size: 5764
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3572 276
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I EcoICRI EcoNI EcoRV FseI
FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII
SacI SanDI SexAI SfiI SrfI SwaI