Restriction map for
pFN21AE2163
(Circular) (Six-base) MAPSORT of: pFN21AE2163.seq Check: 8981 from: 1 to: 7351
pFN21AE2163 7351 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3340 3340
Size: 7351
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5161 278
Size: 53 83 186 936 3625 2468
Fragments arranged by size:
3625 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4694 1937
Size: 2757 4594
Acc65I G'GTAC_C
Cuts at: 3101 4143 4672 3101
Size: 1042 529 5780
Fragments arranged by size:
5780 1042 529
AclI AA'CG_TT
Cuts at: 1563 5478 5851 1563
Size: 3915 373 3063
Fragments arranged by size:
3915 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2609 3416 4568 5414 6798 7066 1632
Size: 977 807 1152 846 1384 268 1917
Fragments arranged by size:
1917 1384 1152 977 846 807 268
AfeI AGC'GCT
Cuts at: 4292 4292
Size: 7351
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7154
AflIII A'CryG_T
Cuts at: 1203 2043 3171 3803 6250 7227 1203
Size: 840 1128 632 2447 977 1327
Fragments arranged by size:
2447 1327 1128 977 840 632
AgeI A'CCGG_T
Cuts at: 6182 6182
Size: 7351
AhdI GACnn_n'nnGTC
Cuts at: 3787 6080 3787
Size: 2293 5058
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7351
AlwNI CAG_nnn'CTG
Cuts at: 2812 3685 4324 4358 5094 6666 2812
Size: 873 639 34 736 1572 3497
Fragments arranged by size:
3497 1572 873 736 639 34
ApaLI G'TGCA_C
Cuts at: 3320 5408 6564 3320
Size: 2088 1156 4107
Fragments arranged by size:
4107 2088 1156
ApoI r'AATT_y
Cuts at: 1493 3086 4660 4886 5038 7147 1493
Size: 1593 1574 226 152 2109 1697
Fragments arranged by size:
2109 1697 1593 1574 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5905 160
Size: 872 4873 1606
Fragments arranged by size:
4873 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7351
AvaI C'yCGr_G
Cuts at: 1870 1943 2159 3304 4517 4536 4676 1870
Size: 73 216 1145 1213 19 140 4545
Fragments arranged by size:
4545 1213 1145 216 140 73 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3085 3118 3085
Size: 33 7318
BamHI G'GATC_C
Cuts at: 4681 5048 4681
Size: 367 6984
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3101 3292 3851 4060 4143
Size: 325 217 627 1314 191 559 209 83
Cuts at: 4143 4455 4672 6127 618
Size: 312 217 1455 1842
Fragments arranged by size:
1842 1455 1314 627 559 325 312 217
217 209 191 83
BanII G_rGCy'C
Cuts at: 1391 2145 3546 4162 4581 4670 1391
Size: 754 1401 616 419 89 4072
Fragments arranged by size:
4072 1401 754 616 419 89
BbeI G_GCGC'C
Cuts at: 3296 4064 3296
Size: 768 6583
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2875 3806 928
Size: 971 976 931 4473
Fragments arranged by size:
4473 976 971 931
BbvCI CC'TCA_GC
Cuts at: 3111 3111
Size: 7351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5542 5576 1903
Size: 34 50 34 3521 34 3678
Fragments arranged by size:
3678 3521 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5243 6453 5243
Size: 1210 6141
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7351
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5962 136
Size: 107 122 71 631 605 4290 1525
Fragments arranged by size:
4290 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3720 7346 3720
Size: 3626 3725
BlpI GC'TnA_GC
Cuts at: 4768 4768
Size: 7351
Bme1580I G_kGCm'C
Cuts at: 1790 2987 3324 4243 5412 6568 1790
Size: 1197 337 919 1169 1156 2573
Fragments arranged by size:
2573 1197 1169 1156 919 337
BmgBI CAC'GTC
Cuts at: 1318 3007 3804 1318
Size: 1689 797 4865
Fragments arranged by size:
4865 1689 797
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2190 2300 4166
Size: 416 53 83 399 24 773 110 1866
Cuts at: 4166 5082 6040 442
Size: 916 958 1753
Fragments arranged by size:
1866 1753 958 916 773 416 399 110
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7351
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4652 4652
Size: 7351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2159 2336 3493 4229 4622 6011 1239
Size: 920 177 1157 736 393 1389 2579
Fragments arranged by size:
2579 1389 1157 920 736 393 177
Bpu10I CC'TnA_GC
Cuts at: 3111 3893 3111
Size: 782 6569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2198 2414 2534 5476 6341 6639 2198
Size: 216 120 2942 865 298 2910
Fragments arranged by size:
2942 2910 865 298 216 120
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3911 6014 882
Size: 617 2412 2103 2219
Fragments arranged by size:
2412 2219 2103 617
BsaAI yAC'GTr
Cuts at: 493 1204 2044 2629 3325 5142 493
Size: 711 840 585 696 1817 2702
Fragments arranged by size:
2702 1817 840 711 696 585
BsaBI GATnn'nnATC
Cuts at: 7345 7345
Size: 7351
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2448 2769 3293 4061
Size: 53 83 186 936 915 321 524 768
Cuts at: 4061 5158 5540 6175 275
Size: 1097 382 635 1451
Fragments arranged by size:
1451 1097 936 915 768 635 524 382
321 186 83 53
BsaWI w'CCGG_w
Cuts at: 2025 5784 6182 6456 6603 7014 2025
Size: 3759 398 274 147 411 2362
Fragments arranged by size:
3759 2362 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2020 2050 3143 3173 3788
Size: 30 165 30 617 30 1093 30 615
Cuts at: 3788 3818 3920 3950 3995 4025 1178
Size: 30 102 30 45 30 4504
Fragments arranged by size:
4504 1093 617 615 165 102 45 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3395 3551 3746 3815 3941 1186
Size: 2209 156 195 69 126 4596
Fragments arranged by size:
4596 2209 195 156 126 69
BseYI C'CCAG_C
Cuts at: 3119 3908 6554 7050 3119
Size: 789 2646 496 3420
Fragments arranged by size:
3420 2646 789 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2243 2555 2769 2774 2804 2855 3347
Size: 573 507 312 214 5 30 51 492
Cuts at: 3347 3638 3716 4400 1163
Size: 291 78 684 4114
Fragments arranged by size:
4114 684 573 507 492 312 291 214
78 51 30 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4801 5562 6590 1540
Size: 275 186 2800 761 1028 2301
Fragments arranged by size:
2800 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2664 3324 3546 3561 4581 5412 5497 6568 2664
Size: 660 222 15 1020 831 85 1071 3447
Fragments arranged by size:
3447 1071 1020 831 660 222 85 15
BsmI GAATG_Cn'
Cuts at: 3264 4862 4955 3264
Size: 1598 93 5660
Fragments arranged by size:
5660 1598 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2664 2987 3324 3546 3561 4162
Size: 399 355 519 323 337 222 15 601
Cuts at: 4162 4243 4581 4670 5412 5497 6568 1391
Size: 81 338 89 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 601 519 399 355 338
337 323 222 89 85 81 15
BspEI T'CCGG_A
Cuts at: 2025 7014 2025
Size: 4989 2362
BspHI T'CATG_A
Cuts at: 3582 5240 6970 3582
Size: 1658 1730 3963
Fragments arranged by size:
3963 1730 1658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3340 3467 4366 4706 844
Size: 2496 127 899 340 3489
Fragments arranged by size:
3489 2496 899 340 127
BsrBI CCG'CTC
Cuts at: 1394 1823 3745 5238 1394
Size: 429 1922 1493 3507
Fragments arranged by size:
3507 1922 1493 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5846 6020 65
Size: 1183 4598 174 1396
Fragments arranged by size:
4598 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2771 4211 5995 6182 1700
Size: 110 961 1440 1784 187 2869
Fragments arranged by size:
2869 1784 1440 961 187 110
BsrGI T'GTAC_A
Cuts at: 96 3624 96
Size: 3528 3823
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7351
BssSI C'ACGA_G
Cuts at: 5411 6423 5411
Size: 1012 6339
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3643 3685 794
Size: 2849 42 4460
Fragments arranged by size:
4460 2849 42
BstBI TT'CG_AA
Cuts at: 6155 6155
Size: 7351
BstEII G'GTnAC_C
Cuts at: 1184 4396 1184
Size: 3212 4139
BstXI CCAn_nnnn'nTGG
Cuts at: 3277 3517 4369 4489 3277
Size: 240 852 120 6139
Fragments arranged by size:
6139 852 240 120
BstYI r'GATC_y
Cuts at: 1972 3720 3930 4681 5048 5433 5450 6902 6988
Size: 1748 210 751 367 385 17 1452 86
Cuts at: 6988 7000 7346 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1748 1452 751 385 367 346 210
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2392 2895 3415 3449 4122 513
Size: 552 1327 503 520 34 673 3742
Fragments arranged by size:
3742 1327 673 552 520 503 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6612
Fragments arranged by size:
6612 683 56
BtsI GCAGTG_nn'
Cuts at: 2786 3294 3683 3855 4867 5661 5681 2786
Size: 508 389 172 1012 794 20 4456
Fragments arranged by size:
4456 1012 794 508 389 172 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7351
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2374 2409 529
Size: 35 1810 35 5471
Fragments arranged by size:
5471 1810 35 35
DraI TTT'AAA
Cuts at: 4655 4655
Size: 7351
DraIII CAC_nnn'GTG
Cuts at: 3643 3643
Size: 7351
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2737 3592 6358 809
Size: 507 1421 855 2766 1802
Fragments arranged by size:
2766 1802 1421 855 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2307 2395 2724
Size: 54 1425 50 275 18 477 88 329
Cuts at: 2724 3219 3946 4798 5687 7316 8
Size: 495 727 852 889 1629 43
Fragments arranged by size:
1629 1425 889 852 727 495 477 329
275 88 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4798 1537
Size: 275 2986 4090
Fragments arranged by size:
4090 2986 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3473 3551 5281 1353
Size: 2120 78 1730 3423
Fragments arranged by size:
3423 2120 1730 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2165 5938 6312 6458 387
Size: 1064 138 576 3773 374 146 1280
Fragments arranged by size:
3773 1280 1064 576 374 146 138
EcoICRI GAG'CTC
Cuts at: 3544 4579 3544
Size: 1035 6316
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2336 2609 3416 3493 4229 4568
Size: 393 527 177 273 807 77 736 339
Cuts at: 4568 4622 5414 6011 6798 7066 1239
Size: 54 792 597 787 268 1524
Fragments arranged by size:
1524 807 792 787 736 597 527 393
339 273 268 177 77 54
EcoO109I rG'GnC_Cy
Cuts at: 2176 2809 3013 3739 3754 4099 4633 2176
Size: 633 204 726 15 345 534 4894
Fragments arranged by size:
4894 726 633 534 345 204 15
EcoRI G'AATT_C
Cuts at: 4660 5038 4660
Size: 378 6973
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2899 4207 2899
Size: 1308 6043
FspI TGC'GCA
Cuts at: 2544 5857 2544
Size: 3313 4038
HaeII r_GCGC'y
Cuts at: 1678 2920 3296 4038 4064 4294 6498 1678
Size: 1242 376 742 26 230 2204 2531
Fragments arranged by size:
2531 2204 1242 742 376 230 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2020 2052 3773 3805 4211
Size: 32 135 32 448 32 1721 32 406
Cuts at: 4211 4243 5992 6024 6066 6098 1373
Size: 32 1749 32 42 32 2626
Fragments arranged by size:
2626 1749 1721 448 406 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2440 4695 4941 669
Size: 988 281 502 2255 246 3079
Fragments arranged by size:
3079 2255 988 502 281 246
HindIII A'AGCT_T
Cuts at: 748 2876 748
Size: 2128 5223
HpaI GTT'AAC
Cuts at: 4941 4941
Size: 7351
KasI G'GCGC_C
Cuts at: 3292 4060 3292
Size: 768 6583
KpnI G_GTAC'C
Cuts at: 3105 4147 4676 3105
Size: 1042 529 5780
Fragments arranged by size:
5780 1042 529
MfeI C'AATT_G
Cuts at: 4950 4950
Size: 7351
MluI A'CGCG_T
Cuts at: 7227 7227
Size: 7351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3621 4026 4038 6465 6649 1305
Size: 2316 405 12 2427 184 2007
Fragments arranged by size:
2427 2316 2007 405 184 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2309 2726 3948 10
Size: 54 1425 343 477 417 1222 3413
Fragments arranged by size:
3413 1425 1222 477 417 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2948 2969 2990 2996 3275 3365 3581
Size: 648 1782 21 21 6 279 90 216
Cuts at: 3581 5309 5668 5827 518
Size: 1728 359 159 2042
Fragments arranged by size:
2042 1782 1728 648 359 279 216 159
90 21 21 6
MspA1I CmG'CkG
Cuts at: 1606 2620 3331 4124 4254 4767 5444 6592 6837
Size: 1014 711 793 130 513 677 1148 245
Cuts at: 6837 1606
Size: 2120
Fragments arranged by size:
2120 1148 1014 793 711 677 513 245
130
NaeI GCC'GGC
Cuts at: 2773 4213 2773
Size: 1440 5911
NarI GG'CG_CC
Cuts at: 3293 4061 3293
Size: 768 6583
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6799
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7351
NgoMIV G'CCGG_C
Cuts at: 2771 4211 2771
Size: 1440 5911
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7351
NotI GC'GGCC_GC
Cuts at: 4798 4798
Size: 7351
NspI r_CATG'y
Cuts at: 1132 1229 2022 2371 2783 3175 3187 3883 4709
Size: 97 793 349 412 392 12 696 826
Cuts at: 4709 6254 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 826 793 696 412 392 349
97 12
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7351
PciI A'CATG_T
Cuts at: 3171 6250 3171
Size: 3079 4272
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2302 2812 4324 1108
Size: 1194 510 1512 4135
Fragments arranged by size:
4135 1512 1194 510
PfoI T'CCnGG_A
Cuts at: 2313 2805 3659 2313
Size: 492 854 6005
Fragments arranged by size:
6005 854 492
PmeI GTTT'AAAC
Cuts at: 4655 4655
Size: 7351
PmlI CAC'GTG
Cuts at: 2044 3325 2044
Size: 1281 6070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5420 5452 6933 6965 5420
Size: 32 1481 32 5806
Fragments arranged by size:
5806 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2176 2809 3739 3754 4099 4633 2176
Size: 633 930 15 345 534 4894
Fragments arranged by size:
4894 930 633 534 345 15
PshAI GACnn'nnGTC
Cuts at: 1613 3771 1613
Size: 2158 5193
PsiI TTA'TAA
Cuts at: 4921 4921
Size: 7351
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3968 4000 3968
Size: 32 7319
PstI C_TGCA'G
Cuts at: 830 2600 3681 4703 830
Size: 1770 1081 1022 3478
Fragments arranged by size:
3478 1770 1081 1022
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7351
PvuII CAG'CTG
Cuts at: 3331 3331
Size: 7351
RsrII CG'GwC_CG
Cuts at: 4126 4126
Size: 7351
SacI G_AGCT'C
Cuts at: 3546 4581 3546
Size: 1035 6316
SacII CC_GC'GG
Cuts at: 4125 4125
Size: 7351
SalI G'TCGA_C
Cuts at: 1936 4693 1936
Size: 2757 4594
SanDI GG'GwC_CC
Cuts at: 2176 2176
Size: 7351
SapI GCTCTTCn'nnn_
Cuts at: 3551 3551
Size: 7351
SbfI CC_TGCA'GG
Cuts at: 4703 4703
Size: 7351
ScaI AGT'ACT
Cuts at: 2477 3713 5599 7311 2477
Size: 1236 1886 1712 2517
Fragments arranged by size:
2517 1886 1712 1236
SexAI A'CCwGG_T
Cuts at: 3639 3639
Size: 7351
SfcI C'TryA_G
Cuts at: 826 1045 2596 3677 4699 5834 6515 6706 826
Size: 219 1551 1081 1022 1135 681 191 1471
Fragments arranged by size:
1551 1471 1135 1081 1022 681 219 191
SfoI GGC'GCC
Cuts at: 3294 4062 3294
Size: 768 6583
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7351
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7351
SmaI CCC'GGG
Cuts at: 1872 4678 1872
Size: 2806 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2213 2429 2549 5455 6356 6618
Size: 197 926 270 216 120 2906 901 262
Cuts at: 6618 820
Size: 1553
Fragments arranged by size:
2906 1553 926 901 270 262 216 197
120
SnaBI TAC'GTA
Cuts at: 493 5142 493
Size: 4649 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7351
SphI G_CATG'C
Cuts at: 1132 2022 2783 3883 4709 1132
Size: 890 761 1100 826 3774
Fragments arranged by size:
3774 1100 890 826 761
SspI AAT'ATT
Cuts at: 5 52 5275 5
Size: 47 5223 2081
Fragments arranged by size:
5223 2081 47
StuI AGG'CCT
Cuts at: 1517 2136 1517
Size: 619 6732
StyI C'CwwG_G
Cuts at: 513 1065 3792 4095 4390 4425 4790 513
Size: 552 2727 303 295 35 365 3074
Fragments arranged by size:
3074 2727 552 365 303 295 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4295 5378 5395 5548 5733 6178 325
Size: 1255 2715 1083 17 153 185 445 1498
Fragments arranged by size:
2715 1498 1255 1083 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2475 3624 3711
Size: 274 80 33 51 1443 498 1149 87
Cuts at: 3711 4185 5597 7309 96
Size: 474 1412 1712 138
Fragments arranged by size:
1712 1443 1412 1149 498 474 274 138
87 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3143 3175 3194 3226 3788 3820 7311 7343 3143
Size: 32 19 32 562 32 3491 32 3151
Fragments arranged by size:
3491 3151 562 32 32 32 32 19
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7351
XbaI T'CTAG_A
Cuts at: 4687 4687
Size: 7351
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2755 3941 4321 2011
Size: 744 1186 380 5041
Fragments arranged by size:
5041 1186 744 380
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7351
XmaI C'CCGG_G
Cuts at: 1870 4676 1870
Size: 2806 4545
XmnI GAAnn'nnTTC
Cuts at: 3089 3152 3713 5480 3089
Size: 63 561 1767 4960
Fragments arranged by size:
4960 1767 561 63
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5159 276
Size: 53 83 186 936 3625 2468
Fragments arranged by size:
3625 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BfrBI BsiWI BsmBI BstZ17I
Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI PacI
PspOMI PspXI SfiI SrfI SwaI