Restriction map for
pFN21AE2157
(Circular) (Six-base) MAPSORT of: pFN21AE2157.seq Check: 7587 from: 1 to: 5695
pFN21AE2157 5695 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3505 278
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3038 1937
Size: 1101 4594
Acc65I G'GTAC_C
Cuts at: 3016 3016
Size: 5695
AclI AA'CG_TT
Cuts at: 1563 3822 4195 1563
Size: 2259 373 3063
Fragments arranged by size:
3063 2259 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2286 3758 5142 5410 1632
Size: 654 1472 1384 268 1917
Fragments arranged by size:
1917 1472 1384 654 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5498
AflIII A'CryG_T
Cuts at: 1203 2753 2951 4594 5571 1203
Size: 1550 198 1643 977 1327
Fragments arranged by size:
1643 1550 1327 977 198
AgeI A'CCGG_T
Cuts at: 4526 4526
Size: 5695
AhdI GACnn_n'nnGTC
Cuts at: 4424 4424
Size: 5695
AleI CACnn'nnGTG
Cuts at: 1166 2248 1166
Size: 1082 4613
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5695
AlwNI CAG_nnn'CTG
Cuts at: 3438 5010 3438
Size: 1572 4123
ApaI G_GGCC'C
Cuts at: 2634 2634
Size: 5695
ApaLI G'TGCA_C
Cuts at: 3752 4908 3752
Size: 1156 4539
ApoI r'AATT_y
Cuts at: 1493 2097 2894 3004 3230 3382 5491 1493
Size: 604 797 110 226 152 2109 1697
Fragments arranged by size:
2109 1697 797 604 226 152 110
AseI AT'TA_AT
Cuts at: 160 1032 4249 160
Size: 872 3217 1606
Fragments arranged by size:
3217 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
AvaI C'yCGr_G
Cuts at: 1870 1943 2811 3020 1870
Size: 73 868 209 4545
Fragments arranged by size:
4545 868 209 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2957 2990 2957
Size: 33 5662
BamHI G'GATC_C
Cuts at: 3025 3392 3025
Size: 367 5328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3016 4471 618
Size: 325 217 627 1229 1455 1842
Fragments arranged by size:
1842 1455 1229 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2540 2634 3014 1391
Size: 1149 94 380 4072
Fragments arranged by size:
4072 1149 380 94
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3886 3920 1903
Size: 34 1949 34 3678
Fragments arranged by size:
3678 1949 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2348 2985 3587 4797 2348
Size: 637 602 1210 3246
Fragments arranged by size:
3246 1210 637 602
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4306 136
Size: 107 122 71 631 605 2634 1525
Fragments arranged by size:
2634 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5690 5690
Size: 5695
BlpI GC'TnA_GC
Cuts at: 3112 3112
Size: 5695
Bme1580I G_kGCm'C
Cuts at: 1790 2634 3756 4912 1790
Size: 844 1122 1156 2573
Fragments arranged by size:
2573 1156 1122 844
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5695
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3426 4384 442
Size: 416 53 83 399 24 2009 958 1753
Fragments arranged by size:
2009 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2673 2990 4355 1239
Size: 1434 317 1365 2579
Fragments arranged by size:
2579 1434 1365 317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2753 3820 4685 4983 2753
Size: 1067 865 298 3465
Fragments arranged by size:
3465 1067 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4358 882
Size: 617 2859 2219
Fragments arranged by size:
2859 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3486 493
Size: 711 2282 2702
Fragments arranged by size:
2702 2282 711
BsaBI GATnn'nnATC
Cuts at: 5689 5689
Size: 5695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3502 3884 4519 275
Size: 53 83 186 936 1969 382 635 1451
Fragments arranged by size:
1969 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4128 4526 4800 4947 5358 4128
Size: 398 274 147 411 4465
Fragments arranged by size:
4465 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5470
Fragments arranged by size:
5470 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5695
BseYI C'CCAG_C
Cuts at: 4898 5394 4898
Size: 496 5199
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5122
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3145 3906 4934 1540
Size: 275 186 1144 761 1028 2301
Fragments arranged by size:
2301 1144 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3756 3841 4912 3756
Size: 85 1071 4539
Fragments arranged by size:
4539 1071 85
BsmI GAATG_Cn'
Cuts at: 3206 3299 3206
Size: 93 5602
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2540 2634 3014 3756 3841 4912 1391
Size: 399 750 94 380 742 85 1071 2174
Fragments arranged by size:
2174 1071 750 742 399 380 94 85
BspEI T'CCGG_A
Cuts at: 5358 5358
Size: 5695
BspHI T'CATG_A
Cuts at: 3584 5314 3584
Size: 1730 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2415 3050 844
Size: 1571 635 3489
Fragments arranged by size:
3489 1571 635
BsrBI CCG'CTC
Cuts at: 1394 1823 3582 1394
Size: 429 1759 3507
Fragments arranged by size:
3507 1759 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2939 4190 4364 65
Size: 1183 1691 1251 174 1396
Fragments arranged by size:
1691 1396 1251 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4339 4526 1700
Size: 110 2529 187 2869
Fragments arranged by size:
2869 2529 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5695
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5695
BssSI C'ACGA_G
Cuts at: 3755 4767 3755
Size: 1012 4683
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2217 794
Size: 1423 4272
BstBI TT'CG_AA
Cuts at: 4499 4499
Size: 5695
BstEII G'GTnAC_C
Cuts at: 1184 2235 1184
Size: 1051 4644
BstYI r'GATC_y
Cuts at: 1972 3025 3392 3777 3794 5246 5332 5344 5690
Size: 1053 367 385 17 1452 86 12 346
Cuts at: 5690 1972
Size: 1977
Fragments arranged by size:
1977 1452 1053 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2092 513
Size: 552 1027 4116
Fragments arranged by size:
4116 1027 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4956
Fragments arranged by size:
4956 683 56
BtsI GCAGTG_nn'
Cuts at: 3211 4005 4025 3211
Size: 794 20 4881
Fragments arranged by size:
4881 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5660
DraI TTT'AAA
Cuts at: 2577 2999 2577
Size: 422 5273
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4702 809
Size: 507 3386 1802
Fragments arranged by size:
3386 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3142 4031 5660
Size: 54 1425 50 275 18 1312 889 1629
Cuts at: 5660 8
Size: 43
Fragments arranged by size:
1629 1425 1312 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3142 1537
Size: 275 1330 4090
Fragments arranged by size:
4090 1330 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3625 1353
Size: 2272 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2442 4282 4656 4802 387
Size: 1064 138 853 1840 374 146 1280
Fragments arranged by size:
1840 1280 1064 853 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 2673 2990 3758 4355 5142 5410
Size: 393 654 387 317 768 597 787 268
Cuts at: 5410 1239
Size: 1524
Fragments arranged by size:
1524 787 768 654 597 393 387 317
268
EcoO109I rG'GnC_Cy
Cuts at: 2631 2631
Size: 5695
EcoRI G'AATT_C
Cuts at: 2097 3004 3382 2097
Size: 907 378 4410
Fragments arranged by size:
4410 907 378
EcoRV GAT'ATC
Cuts at: 2043 2915 2043
Size: 872 4823
FspI TGC'GCA
Cuts at: 4201 4201
Size: 5695
HaeII r_GCGC'y
Cuts at: 1678 4842 1678
Size: 3164 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2485 2517 4336 4368 4410
Size: 32 135 32 913 32 1819 32 42
Cuts at: 4410 4442 1373
Size: 32 2626
Fragments arranged by size:
2626 1819 913 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3039 3285 669
Size: 988 281 1101 246 3079
Fragments arranged by size:
3079 1101 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2864 748
Size: 2116 3579
HpaI GTT'AAC
Cuts at: 3285 3285
Size: 5695
KpnI G_GTAC'C
Cuts at: 3020 3020
Size: 5695
MfeI C'AATT_G
Cuts at: 3294 3294
Size: 5695
MluI A'CGCG_T
Cuts at: 5571 5571
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3002 4809 4993 1305
Size: 1697 1807 184 2007
Fragments arranged by size:
2007 1807 1697 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3873
Fragments arranged by size:
3873 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2248 2602 2785 2799 2845 3653 4012
Size: 648 1082 354 183 14 46 808 359
Cuts at: 4012 4171 518
Size: 159 2042
Fragments arranged by size:
2042 1082 808 648 359 354 183 159
46 14
MspA1I CmG'CkG
Cuts at: 1606 2891 3111 3788 4936 5181 1606
Size: 1285 220 677 1148 245 2120
Fragments arranged by size:
2120 1285 1148 677 245 220
NcoI C'CATG_G
Cuts at: 513 1065 2092 513
Size: 552 1027 4116
Fragments arranged by size:
4116 1027 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5695
NotI GC'GGCC_GC
Cuts at: 3142 3142
Size: 5695
NspI r_CATG'y
Cuts at: 1132 1229 2220 2757 2955 3053 4598 1132
Size: 97 991 537 198 98 1545 2229
Fragments arranged by size:
2229 1545 991 537 198 98 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5695
PciI A'CATG_T
Cuts at: 2753 2951 4594 2753
Size: 198 1643 3854
Fragments arranged by size:
3854 1643 198
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5695
PmeI GTTT'AAAC
Cuts at: 2999 2999
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 5277 5309 3764
Size: 32 1481 32 4150
Fragments arranged by size:
4150 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2770 1613
Size: 1157 4538
PsiI TTA'TAA
Cuts at: 2832 3265 2832
Size: 433 5262
PspOMI G'GGCC_C
Cuts at: 2630 2630
Size: 5695
PstI C_TGCA'G
Cuts at: 830 3047 830
Size: 2217 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5695
PvuII CAG'CTG
Cuts at: 2891 2891
Size: 5695
SalI G'TCGA_C
Cuts at: 1936 3037 1936
Size: 1101 4594
SbfI CC_TGCA'GG
Cuts at: 3047 3047
Size: 5695
ScaI AGT'ACT
Cuts at: 3943 5655 3943
Size: 1712 3983
SfcI C'TryA_G
Cuts at: 826 1045 2142 3043 4178 4859 5050 826
Size: 219 1097 901 1135 681 191 1471
Fragments arranged by size:
1471 1135 1097 901 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5695
SmaI CCC'GGG
Cuts at: 1872 3022 1872
Size: 1150 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2732 3799 4700 4962 820
Size: 197 926 789 1067 901 262 1553
Fragments arranged by size:
1553 1067 926 901 789 262 197
SnaBI TAC'GTA
Cuts at: 493 3486 493
Size: 2993 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5695
SphI G_CATG'C
Cuts at: 1132 3053 1132
Size: 1921 3774
SspI AAT'ATT
Cuts at: 5 52 3619 5
Size: 47 3567 2081
Fragments arranged by size:
3567 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5695
StyI C'CwwG_G
Cuts at: 513 1065 2092 3134 513
Size: 552 1027 1042 3074
Fragments arranged by size:
3074 1042 1027 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3722 3739 3892 4077 4522 325
Size: 1255 2142 17 153 185 445 1498
Fragments arranged by size:
2142 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2461 3941 5653
Size: 274 80 33 51 1443 484 1480 1712
Cuts at: 5653 96
Size: 138
Fragments arranged by size:
1712 1480 1443 484 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5655 5687 5655
Size: 32 5663
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5695
XbaI T'CTAG_A
Cuts at: 3031 3031
Size: 5695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5695
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5695
XmaI C'CCGG_G
Cuts at: 1870 3020 1870
Size: 1150 4545
XmnI GAAnn'nnTTC
Cuts at: 3824 3824
Size: 5695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3503 276
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI