Restriction map for
pFN21AE2156
(Circular) (Six-base) MAPSORT of: pFN21AE2156.seq Check: 8146 from: 1 to: 5962
pFN21AE2156 5962 bp
With 182 enzymes: SgfI *
July 11, 2011 17:14 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2168 3305 1937
Size: 231 1137 4594
Fragments arranged by size:
4594 1137 231
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2148 2522 4025 5409 5677 1632
Size: 516 374 1503 1384 268 1917
Fragments arranged by size:
1917 1503 1384 516 374 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 2504 2713 4861 5838 1203
Size: 1301 209 2148 977 1327
Fragments arranged by size:
2148 1327 1301 977 209
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5962
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2143 2175 2143
Size: 32 5930
AlwNI CAG_nnn'CTG
Cuts at: 2728 3705 5277 2728
Size: 977 1572 3413
Fragments arranged by size:
3413 1572 977
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 2030 2801 3003 3271 3497 3649 5758 1493
Size: 537 771 202 268 226 152 2109 1697
Fragments arranged by size:
2109 1697 771 537 268 226 202 152
AseI AT'TA_AT
Cuts at: 160 1032 2918 4516 160
Size: 872 1886 1598 1606
Fragments arranged by size:
1886 1606 1598 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 2907 3287 1870
Size: 73 964 380 4545
Fragments arranged by size:
4545 964 380 73
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3283 4738 618
Size: 325 217 627 1496 1455 1842
Fragments arranged by size:
1842 1496 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3281 1391
Size: 1890 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3082 928
Size: 971 1183 3808
Fragments arranged by size:
3808 1183 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4153 4187 1903
Size: 34 2216 34 3678
Fragments arranged by size:
3678 2216 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3854 5064 3854
Size: 1210 4752
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5962
BfrBI ATG'CAT
Cuts at: 2279 2279
Size: 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4573 136
Size: 107 122 71 631 605 2901 1525
Fragments arranged by size:
2901 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2131 3109 5957 2131
Size: 978 2848 2136
Fragments arranged by size:
2848 2136 978
BlpI GC'TnA_GC
Cuts at: 3379 3379
Size: 5962
Bme1580I G_kGCm'C
Cuts at: 1790 4023 5179 1790
Size: 2233 1156 2573
Fragments arranged by size:
2573 2233 1156
BmgBI CAC'GTC
Cuts at: 1318 2584 1318
Size: 1266 4696
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3693 4651 442
Size: 416 53 83 399 24 2276 958 1753
Fragments arranged by size:
2276 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2574 1056
Size: 1518 4444
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2663 2663
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4622 1239
Size: 3383 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3240 4087 4952 5250 3240
Size: 847 865 298 3952
Fragments arranged by size:
3952 865 847 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4625 882
Size: 617 3126 2219
Fragments arranged by size:
3126 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2714 3753 493
Size: 711 1510 1039 2702
Fragments arranged by size:
2702 1510 1039 711
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3769 4151 4786 275
Size: 53 83 186 936 2236 382 635 1451
Fragments arranged by size:
2236 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4395 4793 5067 5214 5625 4395
Size: 398 274 147 411 4732
Fragments arranged by size:
4732 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5737
Fragments arranged by size:
5737 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2478 1186
Size: 1292 4670
BseYI C'CCAG_C
Cuts at: 5165 5661 5165
Size: 496 5466
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2073 1163
Size: 573 337 5052
Fragments arranged by size:
5052 573 337
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2658 3412 4173 5201 1540
Size: 275 186 657 754 761 1028 2301
Fragments arranged by size:
2301 1028 761 754 657 275 186
BsiHKAI G_wGCw'C
Cuts at: 2767 4023 4108 5179 2767
Size: 1256 85 1071 3550
Fragments arranged by size:
3550 1256 1071 85
BsmI GAATG_Cn'
Cuts at: 3473 3566 3473
Size: 93 5869
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2767 3281 4023 4108 5179 1391
Size: 399 977 514 742 85 1071 2174
Fragments arranged by size:
2174 1071 977 742 514 399 85
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 2527 3851 5581 2527
Size: 1324 1730 2908
Fragments arranged by size:
2908 1730 1324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3317 844
Size: 2473 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3849 1394
Size: 429 2026 3507
Fragments arranged by size:
3507 2026 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4457 4631 65
Size: 1183 3209 174 1396
Fragments arranged by size:
3209 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3231 4606 4793 1700
Size: 110 1421 1375 187 2869
Fragments arranged by size:
2869 1421 1375 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5962
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 4022 5034 4022
Size: 1012 4950
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5962
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 2131 2223 2833 3109 3292 3659 4044 4061
Size: 159 92 610 276 183 367 385 17
Cuts at: 4061 5513 5599 5611 5957 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 610 385 367 346 276 183
159 92 86 17 12
BstZ17I GTA'TAC
Cuts at: 2169 2169
Size: 5962
BtgI C'CryG_G
Cuts at: 513 1065 2004 2758 513
Size: 552 939 754 3717
Fragments arranged by size:
3717 939 754 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 3478 4272 4292 2061
Size: 1417 794 20 3731
Fragments arranged by size:
3731 1417 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4969 809
Size: 507 3653 1802
Fragments arranged by size:
3653 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2708 3409 4298
Size: 54 1425 50 275 18 878 701 889
Cuts at: 4298 5927 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 878 701 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3409 1537
Size: 275 1597 4090
Fragments arranged by size:
4090 1597 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3195 3892 1353
Size: 1842 697 3423
Fragments arranged by size:
3423 1842 697
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2522 4025 4622 5409 5677 1239
Size: 393 516 374 1503 597 787 268 1524
Fragments arranged by size:
1524 1503 787 597 516 393 374 268
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 5109 1678
Size: 3431 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2722 2754 3115 3147 4603
Size: 32 135 32 1150 32 361 32 1456
Cuts at: 4603 4635 4677 4709 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1456 1150 361 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2256 3306 3552 669
Size: 988 281 318 1050 246 3079
Fragments arranged by size:
3079 1050 988 318 281 246
HindIII A'AGCT_T
Cuts at: 748 2879 748
Size: 2131 3831
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 2100 3561 2100
Size: 1461 4501
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2480 5076 5260 1305
Size: 1175 2596 184 2007
Fragments arranged by size:
2596 2007 1175 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4140
Fragments arranged by size:
4140 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3920 4279 4438 518
Size: 648 2754 359 159 2042
Fragments arranged by size:
2754 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2828 3196 3378 4055 5203 5448 1606
Size: 1222 368 182 677 1148 245 2120
Fragments arranged by size:
2120 1222 1148 677 368 245 182
NcoI C'CATG_G
Cuts at: 513 1065 2004 2758 513
Size: 552 939 754 3717
Fragments arranged by size:
3717 939 754 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 2570 1052
Size: 1518 4444
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NsiI A_TGCA'T
Cuts at: 2281 2281
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 2508 3184 3320 4865 1132
Size: 97 1279 676 136 1545 2229
Fragments arranged by size:
2229 1545 1279 676 136 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5962
PciI A'CATG_T
Cuts at: 2504 4861 2504
Size: 2357 3605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5962
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PmlI CAC'GTG
Cuts at: 2714 2714
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PstI C_TGCA'G
Cuts at: 830 3314 830
Size: 2484 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
PvuII CAG'CTG
Cuts at: 3196 3196
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SfcI C'TryA_G
Cuts at: 826 1045 2808 3055 3310 4445 5126 5317 826
Size: 219 1763 247 255 1135 681 191 1471
Fragments arranged by size:
1763 1471 1135 681 255 247 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 2909 3289 1872
Size: 1037 380 4545
Fragments arranged by size:
4545 1037 380
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3255 4066 4967 5229 820
Size: 197 926 1312 811 901 262 1553
Fragments arranged by size:
1553 1312 926 901 811 262 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 3217 152
Size: 3065 2897
SphI G_CATG'C
Cuts at: 1132 3184 3320 1132
Size: 2052 136 3774
Fragments arranged by size:
3774 2052 136
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5962
StyI C'CwwG_G
Cuts at: 513 1065 2004 2758 3401 513
Size: 552 939 754 643 3074
Fragments arranged by size:
3074 939 754 643 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3989 4006 4159 4344 4789 325
Size: 1255 2409 17 153 185 445 1498
Fragments arranged by size:
2409 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2076 4208 5920
Size: 274 80 33 51 1443 99 2132 1712
Cuts at: 5920 96
Size: 138
Fragments arranged by size:
2132 1712 1443 274 138 99 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2788 2820 5922 5954 2788
Size: 32 3102 32 2796
Fragments arranged by size:
3102 2796 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5962
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2180 2180
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 2907 3287 1870
Size: 1037 380 4545
Fragments arranged by size:
4545 1037 380
XmnI GAAnn'nnTTC
Cuts at: 2378 2535 2781 3006 4091 2378
Size: 157 246 225 1085 4249
Fragments arranged by size:
4249 1085 246 225 157
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI