Restriction map for
pFN21AE2155
(Circular) (Six-base) MAPSORT of: pFN21AE2155.seq Check: 8724 from: 1 to: 5839
pFN21AE2155 5839 bp
With 182 enzymes: SgfI *
August 25, 2016 14:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2934 3649 278
Size: 53 83 186 936 1398 715 2468
Fragments arranged by size:
2468 1398 936 715 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3182 1937
Size: 1245 4594
Acc65I G'GTAC_C
Cuts at: 3160 3160
Size: 5839
AclI AA'CG_TT
Cuts at: 1563 3966 4339 1563
Size: 2403 373 3063
Fragments arranged by size:
3063 2403 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2410 3902 5286 5554 1632
Size: 778 1492 1384 268 1917
Fragments arranged by size:
1917 1492 1384 778 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5642
AflIII A'CryG_T
Cuts at: 1203 4738 5715 1203
Size: 3535 977 1327
Fragments arranged by size:
3535 1327 977
AgeI A'CCGG_T
Cuts at: 4670 4670
Size: 5839
AhdI GACnn_n'nnGTC
Cuts at: 4568 4568
Size: 5839
AleI CACnn'nnGTG
Cuts at: 1166 2117 1166
Size: 951 4888
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5839
AlwNI CAG_nnn'CTG
Cuts at: 3582 5154 3582
Size: 1572 4267
ApaLI G'TGCA_C
Cuts at: 3896 5052 3896
Size: 1156 4683
ApoI r'AATT_y
Cuts at: 1493 3148 3374 3526 5635 1493
Size: 1655 226 152 2109 1697
Fragments arranged by size:
2109 1697 1655 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4393 160
Size: 872 3361 1606
Fragments arranged by size:
3361 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
AvaI C'yCGr_G
Cuts at: 1870 1943 3164 1870
Size: 73 1221 4545
Fragments arranged by size:
4545 1221 73
BamHI G'GATC_C
Cuts at: 3169 3536 3169
Size: 367 5472
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3160 4615 618
Size: 325 217 627 1373 1455 1842
Fragments arranged by size:
1842 1455 1373 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2586 2904 3158 1391
Size: 1195 318 254 4072
Fragments arranged by size:
4072 1195 318 254
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2365 2809 2818 3063 928
Size: 971 466 444 9 245 3704
Fragments arranged by size:
3704 971 466 444 245 9
BbvCI CC'TCA_GC
Cuts at: 3044 3044
Size: 5839
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4030 4064 1903
Size: 34 2093 34 3678
Fragments arranged by size:
3678 2093 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2547 3731 4941 2547
Size: 1184 1210 3445
Fragments arranged by size:
3445 1210 1184
BclI T'GATC_A
Cuts at: 1548 2796 1548
Size: 1248 4591
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2226 4450 136
Size: 107 122 71 631 605 554 2224 1525
Fragments arranged by size:
2224 1525 631 605 554 122 107 71
BglII A'GATC_T
Cuts at: 5834 5834
Size: 5839
BlpI GC'TnA_GC
Cuts at: 3256 3256
Size: 5839
Bme1580I G_kGCm'C
Cuts at: 1790 2512 3900 5056 1790
Size: 722 1388 1156 2573
Fragments arranged by size:
2573 1388 1156 722
BmgBI CAC'GTC
Cuts at: 1318 2161 1318
Size: 843 4996
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2234 3131 3570
Size: 416 53 83 399 24 817 897 439
Cuts at: 3570 4528 442
Size: 958 1753
Fragments arranged by size:
1753 958 897 817 439 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2585 2741 4499 1239
Size: 1346 156 1758 2579
Fragments arranged by size:
2579 1758 1346 156
Bpu10I CC'TnA_GC
Cuts at: 3044 3044
Size: 5839
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2255 3964 4829 5127 2255
Size: 1709 865 298 2967
Fragments arranged by size:
2967 1709 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3110 4502 882
Size: 617 1611 1392 2219
Fragments arranged by size:
2219 1611 1392 617
BsaAI yAC'GTr
Cuts at: 493 1204 2470 3630 493
Size: 711 1266 1160 2702
Fragments arranged by size:
2702 1266 1160 711
BsaBI GATnn'nnATC
Cuts at: 2690 5833 2690
Size: 3143 2696
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2931 2994 3646 4028
Size: 53 83 186 936 1398 63 652 382
Cuts at: 4028 4663 275
Size: 635 1451
Fragments arranged by size:
1451 1398 936 652 635 382 186 83
63 53
BsaWI w'CCGG_w
Cuts at: 4272 4670 4944 5091 5502 4272
Size: 398 274 147 411 4609
Fragments arranged by size:
4609 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2599 2629 1178
Size: 30 165 30 1196 30 4388
Fragments arranged by size:
4388 1196 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2620 3085 1186
Size: 1434 465 3940
Fragments arranged by size:
3940 1434 465
BseYI C'CCAG_C
Cuts at: 5042 5538 5042
Size: 496 5343
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5266
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3289 4050 5078 1540
Size: 275 186 1288 761 1028 2301
Fragments arranged by size:
2301 1288 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2150 2571 2586 2904 3900 3985 5056 2150
Size: 421 15 318 996 85 1071 2933
Fragments arranged by size:
2933 1071 996 421 318 85 15
BsmI GAATG_Cn'
Cuts at: 3350 3443 3350
Size: 93 5746
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2150 2512 2571 2586 2904 3158 3900
Size: 399 360 362 59 15 318 254 742
Cuts at: 3900 3985 5056 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 362 360 318 254
85 59 15
BspEI T'CCGG_A
Cuts at: 5502 5502
Size: 5839
BspHI T'CATG_A
Cuts at: 3728 5458 3728
Size: 1730 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2301 3194 844
Size: 1457 893 3489
Fragments arranged by size:
3489 1457 893
BsrBI CCG'CTC
Cuts at: 1394 1823 3726 1394
Size: 429 1903 3507
Fragments arranged by size:
3507 1903 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2236 4334 4508 65
Size: 1183 988 2098 174 1396
Fragments arranged by size:
2098 1396 1183 988 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4483 4670 1700
Size: 110 2673 187 2869
Fragments arranged by size:
2869 2673 187 110
BsrGI T'GTAC_A
Cuts at: 96 2589 96
Size: 2493 3346
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5839
BssSI C'ACGA_G
Cuts at: 3899 4911 3899
Size: 1012 4827
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2626 794
Size: 1832 4007
BstBI TT'CG_AA
Cuts at: 4643 4643
Size: 5839
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5839
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2677 3106 2011
Size: 666 429 4744
Fragments arranged by size:
4744 666 429
BstYI r'GATC_y
Cuts at: 1972 3169 3536 3921 3938 5390 5476 5488 5834
Size: 1197 367 385 17 1452 86 12 346
Cuts at: 5834 1972
Size: 1977
Fragments arranged by size:
1977 1452 1197 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4348
Fragments arranged by size:
4348 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2639 2644 2785 2905 487
Size: 683 56 1413 5 141 120 3421
Fragments arranged by size:
3421 1413 683 141 120 56 5
BtsI GCAGTG_nn'
Cuts at: 2620 3071 3355 4149 4169 2620
Size: 451 284 794 20 4290
Fragments arranged by size:
4290 794 451 284 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5804
DraI TTT'AAA
Cuts at: 2173 3143 2173
Size: 970 4869
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4846 809
Size: 507 3530 1802
Fragments arranged by size:
3530 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 2472 2715
Size: 54 1425 50 275 18 528 114 243
Cuts at: 2715 2868 3286 4175 5804 8
Size: 153 418 889 1629 43
Fragments arranged by size:
1629 1425 889 528 418 275 243 153
114 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3286 1537
Size: 275 1474 4090
Fragments arranged by size:
4090 1474 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2327 3020 3029 3769 1353
Size: 974 693 9 740 3423
Fragments arranged by size:
3423 974 740 693 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4426 4800 4946 387
Size: 1064 138 2837 374 146 1280
Fragments arranged by size:
2837 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2584 2902 2584
Size: 318 5521
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2410 2585 2741 3902 4499 5286 5554
Size: 393 778 175 156 1161 597 787 268
Cuts at: 5554 1239
Size: 1524
Fragments arranged by size:
1524 1161 787 778 597 393 268 175
156
EcoNI CCTnn'n_nnAGG
Cuts at: 2756 3047 2756
Size: 291 5548
EcoO109I rG'GnC_Cy
Cuts at: 3031 3031
Size: 5839
EcoRI G'AATT_C
Cuts at: 3148 3526 3148
Size: 378 5461
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2416 2437 2416
Size: 21 5818
FspI TGC'GCA
Cuts at: 4345 4345
Size: 5839
HaeII r_GCGC'y
Cuts at: 1678 3094 4986 1678
Size: 1416 1892 2531
Fragments arranged by size:
2531 1892 1416
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2622 2654 4480 4512 4554
Size: 32 135 32 1050 32 1826 32 42
Cuts at: 4554 4586 1373
Size: 32 2626
Fragments arranged by size:
2626 1826 1050 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2247 2968 3183 3429 669
Size: 988 281 309 721 215 246 3079
Fragments arranged by size:
3079 988 721 309 281 246 215
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5839
HpaI GTT'AAC
Cuts at: 3429 3429
Size: 5839
KpnI G_GTAC'C
Cuts at: 3164 3164
Size: 5839
MfeI C'AATT_G
Cuts at: 3438 3438
Size: 5839
MluI A'CGCG_T
Cuts at: 5715 5715
Size: 5839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4953 5137 1305
Size: 3648 184 2007
Fragments arranged by size:
3648 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2474 2717 2870 10
Size: 54 1425 343 642 243 153 2979
Fragments arranged by size:
2979 1425 642 343 243 153 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 2675 2792 2804 3104 3797 4156
Size: 648 951 558 117 12 300 693 359
Cuts at: 4156 4315 518
Size: 159 2042
Fragments arranged by size:
2042 951 693 648 558 359 300 159
117 12
MspA1I CmG'CkG
Cuts at: 1606 2833 2958 3255 3932 5080 5325 1606
Size: 1227 125 297 677 1148 245 2120
Fragments arranged by size:
2120 1227 1148 677 297 245 125
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4348
Fragments arranged by size:
4348 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5839
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5839
NotI GC'GGCC_GC
Cuts at: 3286 3286
Size: 5839
NspI r_CATG'y
Cuts at: 1132 1229 2437 2654 3197 4742 1132
Size: 97 1208 217 543 1545 2229
Fragments arranged by size:
2229 1545 1208 543 217 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5839
PciI A'CATG_T
Cuts at: 4738 4738
Size: 5839
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2367 2866 1108
Size: 1259 499 4081
Fragments arranged by size:
4081 1259 499
PmeI GTTT'AAAC
Cuts at: 3143 3143
Size: 5839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3908 3940 5421 5453 3908
Size: 32 1481 32 4294
Fragments arranged by size:
4294 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3031 3031
Size: 5839
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5839
PsiI TTA'TAA
Cuts at: 3409 3409
Size: 5839
PstI C_TGCA'G
Cuts at: 830 3191 830
Size: 2361 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5839
PvuII CAG'CTG
Cuts at: 2833 2833
Size: 5839
SacI G_AGCT'C
Cuts at: 2586 2904 2586
Size: 318 5521
SalI G'TCGA_C
Cuts at: 1936 3181 1936
Size: 1245 4594
SapI GCTCTTCn'nnn_
Cuts at: 3029 3029
Size: 5839
SbfI CC_TGCA'GG
Cuts at: 3191 3191
Size: 5839
ScaI AGT'ACT
Cuts at: 4087 5799 4087
Size: 1712 4127
SfcI C'TryA_G
Cuts at: 826 1045 2350 2938 3187 4322 5003 5194 826
Size: 219 1305 588 249 1135 681 191 1471
Fragments arranged by size:
1471 1305 1135 681 588 249 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5839
SmaI CCC'GGG
Cuts at: 1872 3166 1872
Size: 1294 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2270 3943 4844 5106 820
Size: 197 926 327 1673 901 262 1553
Fragments arranged by size:
1673 1553 926 901 327 262 197
SnaBI TAC'GTA
Cuts at: 493 3630 493
Size: 3137 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5839
SphI G_CATG'C
Cuts at: 1132 2437 3197 1132
Size: 1305 760 3774
Fragments arranged by size:
3774 1305 760
SspI AAT'ATT
Cuts at: 5 52 3763 5
Size: 47 3711 2081
Fragments arranged by size:
3711 2081 47
StuI AGG'CCT
Cuts at: 1517 2660 1517
Size: 1143 4696
StyI C'CwwG_G
Cuts at: 513 1065 2004 3278 513
Size: 552 939 1274 3074
Fragments arranged by size:
3074 1274 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2297 3866 3883 4036 4221 4666 325
Size: 1255 717 1569 17 153 185 445 1498
Fragments arranged by size:
1569 1498 1255 717 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2196 2589 4085
Size: 274 80 33 51 1443 219 393 1496
Cuts at: 4085 5797 96
Size: 1712 138
Fragments arranged by size:
1712 1496 1443 393 274 219 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2599 2631 5799 5831 2599
Size: 32 3168 32 2607
Fragments arranged by size:
3168 2607 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5839
XbaI T'CTAG_A
Cuts at: 3175 3175
Size: 5839
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5839
XmaI C'CCGG_G
Cuts at: 1870 3164 1870
Size: 1294 4545
XmnI GAAnn'nnTTC
Cuts at: 3968 3968
Size: 5839
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2932 3647 276
Size: 53 83 186 936 1398 715 2468
Fragments arranged by size:
2468 1398 936 715 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI