Restriction map for
pFN21AE2153
(Circular) (Six-base) MAPSORT of: pFN21AE2153.seq Check: 4378 from: 1 to: 6544
pFN21AE2153 6544 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2296 3058 2296
Size: 762 5782
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4354 278
Size: 53 83 186 936 2818 2468
Fragments arranged by size:
2818 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3714 3887 1937
Size: 1777 173 4594
Fragments arranged by size:
4594 1777 173
Acc65I G'GTAC_C
Cuts at: 2760 3675 3803 3865 2760
Size: 915 128 62 5439
Fragments arranged by size:
5439 915 128 62
AclI AA'CG_TT
Cuts at: 1563 4671 5044 1563
Size: 3108 373 3063
Fragments arranged by size:
3108 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4607 5991 6259 1632
Size: 2975 1384 268 1917
Fragments arranged by size:
2975 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6347
AflIII A'CryG_T
Cuts at: 1203 3301 5443 6420 1203
Size: 2098 2142 977 1327
Fragments arranged by size:
2142 2098 1327 977
AgeI A'CCGG_T
Cuts at: 5375 5375
Size: 6544
AhdI GACnn_n'nnGTC
Cuts at: 5273 5273
Size: 6544
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6544
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2546 2575 1184
Size: 1362 29 5153
Fragments arranged by size:
5153 1362 29
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2243 2275 2243
Size: 32 6512
AlwNI CAG_nnn'CTG
Cuts at: 2225 2548 2905 3648 3704 4287 5859 2225
Size: 323 357 743 56 583 1572 2910
Fragments arranged by size:
2910 1572 743 583 357 323 56
ApaI G_GGCC'C
Cuts at: 2086 2112 2174 2490 2857 3069 3119 3576 3612
Size: 26 62 316 367 212 50 457 36
Cuts at: 3612 2086
Size: 5018
Fragments arranged by size:
5018 457 367 316 212 62 50 36
26
ApaLI G'TGCA_C
Cuts at: 4601 5757 4601
Size: 1156 5388
ApoI r'AATT_y
Cuts at: 1493 3853 4079 4231 6340 1493
Size: 2360 226 152 2109 1697
Fragments arranged by size:
2360 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5098 160
Size: 872 4066 1606
Fragments arranged by size:
4066 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6544
AvaI C'yCGr_G
Cuts at: 1870 1943 2085 2102 2210 2283 2490 2624 3611
Size: 73 142 17 108 73 207 134 987
Cuts at: 3611 3869 1870
Size: 258 4545
Fragments arranged by size:
4545 987 258 207 142 134 108 73
73 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3756 3789 3756
Size: 33 6511
BamHI G'GATC_C
Cuts at: 2836 3874 4241 2836
Size: 1038 367 5139
Fragments arranged by size:
5139 1038 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 2629 2760 2780 2801
Size: 325 217 627 222 620 131 20 21
Cuts at: 2801 3046 3058 3209 3675 3788 3803 3865 5320
Size: 245 12 151 466 113 15 62 1455
Cuts at: 5320 618
Size: 1842
Fragments arranged by size:
1842 1455 627 620 466 325 245 222
217 151 131 113 62 21 20 15
12
BanII G_rGCy'C
Cuts at: 1391 2086 2112 2174 2490 2701 2857 2967 3069
Size: 695 26 62 316 211 156 110 102
Cuts at: 3069 3119 3471 3576 3612 3863 1391
Size: 50 352 105 36 251 4072
Fragments arranged by size:
4072 695 352 316 251 211 156 110
105 102 62 50 36 26
BbeI G_GCGC'C
Cuts at: 2013 2013
Size: 6544
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2266 928
Size: 971 367 5206
Fragments arranged by size:
5206 971 367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4735 4769 1903
Size: 34 2798 34 3678
Fragments arranged by size:
3678 2798 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2245 4436 5646 2245
Size: 2191 1210 3143
Fragments arranged by size:
3143 2191 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6544
BfrBI ATG'CAT
Cuts at: 3727 3727
Size: 6544
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2690 2882 5155
Size: 107 122 71 631 605 1018 192 2273
Cuts at: 5155 136
Size: 1525
Fragments arranged by size:
2273 1525 1018 631 605 192 122 107
71
BglII A'GATC_T
Cuts at: 6539 6539
Size: 6544
BlpI GC'TnA_GC
Cuts at: 2709 3470 3961 2709
Size: 761 491 5292
Fragments arranged by size:
5292 761 491
Bme1580I G_kGCm'C
Cuts at: 1790 2086 2112 2174 2490 2625 2785 2857 2998
Size: 296 26 62 316 135 160 72 141
Cuts at: 2998 3061 3069 3119 3212 3280 3576 3612 3640
Size: 63 8 50 93 68 296 36 28
Cuts at: 3640 3694 4605 5761 1790
Size: 54 911 1156 2573
Fragments arranged by size:
2573 1156 911 316 296 296 160 141
135 93 72 68 63 62 54 50
36 28 26 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6544
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2240 2251 2564
Size: 416 53 83 399 24 823 11 313
Cuts at: 2564 4275 5233 442
Size: 1711 958 1753
Fragments arranged by size:
1753 1711 958 823 416 399 313 83
53 24 11
BmtI G_CTAG'C
Cuts at: 1056 3347 1056
Size: 2291 4253
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3394 3490 3394
Size: 96 6448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2447 2715 3347 3561 5204 1239
Size: 1208 268 632 214 1643 2579
Fragments arranged by size:
2579 1643 1208 632 268 214
Bpu10I CC'TnA_GC
Cuts at: 3697 3697
Size: 6544
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2324 4669 5534 5832 2324
Size: 2345 865 298 3036
Fragments arranged by size:
3036 2345 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5207 882
Size: 617 3708 2219
Fragments arranged by size:
3708 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2890 4335 493
Size: 711 1686 1445 2702
Fragments arranged by size:
2702 1686 1445 711
BsaBI GATnn'nnATC
Cuts at: 6538 6538
Size: 6544
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 4351 4733 5368
Size: 53 83 186 936 477 2341 382 635
Cuts at: 5368 275
Size: 1451
Fragments arranged by size:
2341 1451 936 635 477 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2670 4977 5375 5649 5796 6207 2670
Size: 2307 398 274 147 411 3007
Fragments arranged by size:
3007 2307 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2378 2408 3342 3372 3537
Size: 30 165 30 975 30 934 30 165
Cuts at: 3537 3567 1178
Size: 30 4155
Fragments arranged by size:
4155 975 934 165 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3558 1186
Size: 2372 4172
BseYI C'CCAG_C
Cuts at: 2079 2178 2850 3004 3084 3486 3526 3641 5747
Size: 99 672 154 80 402 40 115 2106
Cuts at: 5747 6243 2079
Size: 496 2380
Fragments arranged by size:
2380 2106 672 496 402 154 115 99
80 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2211 2324 2330 2797 2918 3674 1163
Size: 573 475 113 6 467 121 756 4033
Fragments arranged by size:
4033 756 573 475 467 121 113 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2758 3073 3994 4755 5783 1540
Size: 275 186 757 315 921 761 1028 2301
Fragments arranged by size:
2301 1028 921 761 757 315 275 186
BsiHKAI G_wGCw'C
Cuts at: 2340 2701 3471 4605 4690 5761 2340
Size: 361 770 1134 85 1071 3123
Fragments arranged by size:
3123 1134 1071 770 361 85
BsmI GAATG_Cn'
Cuts at: 4055 4148 4055
Size: 93 6451
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2112 2174 2340 2490 2625 2701
Size: 399 296 26 62 166 150 135 76
Cuts at: 2701 2785 2857 2967 2998 3061 3069 3119 3212
Size: 84 72 110 31 63 8 50 93
Cuts at: 3212 3280 3471 3576 3612 3640 3694 3863 4605
Size: 68 191 105 36 28 54 169 742
Cuts at: 4605 4690 5761 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 296 191 169 166
150 135 110 105 93 85 84 76
72 68 63 62 54 50 36 31
28 26 8
BspEI T'CCGG_A
Cuts at: 6207 6207
Size: 6544
BspHI T'CATG_A
Cuts at: 3822 4433 6163 3822
Size: 611 1730 4203
Fragments arranged by size:
4203 1730 611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2296 3058 3899 844
Size: 1452 762 841 3489
Fragments arranged by size:
3489 1452 841 762
BsrBI CCG'CTC
Cuts at: 1394 1823 3696 4431 1394
Size: 429 1873 735 3507
Fragments arranged by size:
3507 1873 735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2158 2989 3138 5039 5213 65
Size: 1183 910 831 149 1901 174 1396
Fragments arranged by size:
1901 1396 1183 910 831 174 149
BsrFI r'CCGG_y
Cuts at: 1700 1810 2159 2369 3171 5188 5375 1700
Size: 110 349 210 802 2017 187 2869
Fragments arranged by size:
2869 2017 802 349 210 187 110
BsrGI T'GTAC_A
Cuts at: 96 3298 96
Size: 3202 3342
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6544
BssSI C'ACGA_G
Cuts at: 4604 5616 4604
Size: 1012 5532
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3275 794
Size: 2481 4063
BstBI TT'CG_AA
Cuts at: 3835 5348 3835
Size: 1513 5031
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6544
BstXI CCAn_nnnn'nTGG
Cuts at: 2917 2917
Size: 6544
BstYI r'GATC_y
Cuts at: 1972 2836 3874 4241 4626 4643 6095 6181 6193
Size: 864 1038 367 385 17 1452 86 12
Cuts at: 6193 6539 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1038 864 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2015 2015
Size: 6544
BtgI C'CryG_G
Cuts at: 513 1065 2004 2331 2685 3144 3364 513
Size: 552 939 327 354 459 220 3693
Fragments arranged by size:
3693 939 552 459 354 327 220
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5805
Fragments arranged by size:
5805 683 56
BtsI GCAGTG_nn'
Cuts at: 2607 2809 2814 2928 3694 4060 4854 4874 2607
Size: 202 5 114 766 366 794 20 4277
Fragments arranged by size:
4277 794 766 366 202 114 20 5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6544
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3781 3816 529
Size: 35 3217 35 3257
Fragments arranged by size:
3257 3217 35 35
DraI TTT'AAA
Cuts at: 3848 3848
Size: 6544
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5551 809
Size: 507 4235 1802
Fragments arranged by size:
4235 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2682 3070 3429
Size: 54 1425 50 275 18 852 388 359
Cuts at: 3429 3991 4880 6509 8
Size: 562 889 1629 43
Fragments arranged by size:
1629 1425 889 852 562 388 359 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3070 3991 1537
Size: 275 1258 921 4090
Fragments arranged by size:
4090 1258 921 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3821 4474 1353
Size: 2468 653 3423
Fragments arranged by size:
3423 2468 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5131 5505 5651 387
Size: 1064 138 3542 374 146 1280
Fragments arranged by size:
3542 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2699 3469 2699
Size: 770 5774
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2447 2715 3347 3561 4607 5204 5991
Size: 393 815 268 632 214 1046 597 787
Cuts at: 5991 6259 1239
Size: 268 1524
Fragments arranged by size:
1524 1046 815 787 632 597 393 268
268 214
EcoNI CCTnn'n_nnAGG
Cuts at: 3543 3543
Size: 6544
EcoO109I rG'GnC_Cy
Cuts at: 2108 2109 2170 2171 2215 2486 2487 2503 2933
Size: 1 61 1 44 271 1 16 430
Cuts at: 2933 3065 3116 3252 3548 3572 3573 3608 3701
Size: 132 51 136 296 24 1 35 93
Cuts at: 3701 2108
Size: 4951
Fragments arranged by size:
4951 430 296 271 136 132 93 61
51 44 35 24 16 1 1 1
1
EcoRI G'AATT_C
Cuts at: 3853 4231 3853
Size: 378 6166
FspI TGC'GCA
Cuts at: 5050 5050
Size: 6544
HaeII r_GCGC'y
Cuts at: 1678 2013 2317 3648 5691 1678
Size: 335 304 1331 2043 2531
Fragments arranged by size:
2531 2043 1331 335 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2821 2853 3537 3569 5185
Size: 32 135 32 1249 32 684 32 1616
Cuts at: 5185 5217 5259 5291 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1616 1249 684 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2646 3888 4134 669
Size: 988 281 708 1242 246 3079
Fragments arranged by size:
3079 1242 988 708 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6544
HpaI GTT'AAC
Cuts at: 4134 4134
Size: 6544
KasI G'GCGC_C
Cuts at: 2009 2009
Size: 6544
KpnI G_GTAC'C
Cuts at: 2764 3679 3807 3869 2764
Size: 915 128 62 5439
Fragments arranged by size:
5439 915 128 62
MfeI C'AATT_G
Cuts at: 4143 4143
Size: 6544
MluI A'CGCG_T
Cuts at: 6420 6420
Size: 6544
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2927 5658 5842 1305
Size: 1622 2731 184 2007
Fragments arranged by size:
2731 2007 1622 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4722
Fragments arranged by size:
4722 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 2892 3143 3398 4502 4861 5020
Size: 648 1362 364 251 255 1104 359 159
Cuts at: 5020 518
Size: 2042
Fragments arranged by size:
2042 1362 1104 648 364 359 255 251
159
MspA1I CmG'CkG
Cuts at: 1606 2047 2135 2427 2822 2850 2917 3088 3219
Size: 441 88 292 395 28 67 171 131
Cuts at: 3219 3366 3438 3490 3672 3960 4637 5785 6030
Size: 147 72 52 182 288 677 1148 245
Cuts at: 6030 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 441 395 292 288 245
182 171 147 131 88 72 67 52
28
NaeI GCC'GGC
Cuts at: 2371 2371
Size: 6544
NarI GG'CG_CC
Cuts at: 2010 2010
Size: 6544
NcoI C'CATG_G
Cuts at: 513 1065 2004 3144 513
Size: 552 939 1140 3913
Fragments arranged by size:
3913 1140 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6544
NgoMIV G'CCGG_C
Cuts at: 2369 2369
Size: 6544
NheI G'CTAG_C
Cuts at: 1052 3343 1052
Size: 2291 4253
NotI GC'GGCC_GC
Cuts at: 3991 3991
Size: 6544
NsiI A_TGCA'T
Cuts at: 3729 3729
Size: 6544
NspI r_CATG'y
Cuts at: 1132 1229 3305 3662 3727 3731 3902 5447 1132
Size: 97 2076 357 65 4 171 1545 2229
Fragments arranged by size:
2229 2076 1545 357 171 97 65 4
PasI CC'CwG_GG
Cuts at: 1348 2483 2957 1348
Size: 1135 474 4935
Fragments arranged by size:
4935 1135 474
PciI A'CATG_T
Cuts at: 3301 5443 3301
Size: 2142 4402
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3441 3493 3648 1108
Size: 2333 52 155 4004
Fragments arranged by size:
4004 2333 155 52
PmeI GTTT'AAAC
Cuts at: 3848 3848
Size: 6544
PmlI CAC'GTG
Cuts at: 2890 2890
Size: 6544
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4613 4645 6126 6158 4613
Size: 32 1481 32 4999
Fragments arranged by size:
4999 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3252 3252
Size: 6544
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6544
PsiI TTA'TAA
Cuts at: 4114 4114
Size: 6544
PspOMI G'GGCC_C
Cuts at: 2082 2108 2170 2486 2853 3065 3115 3572 3608
Size: 26 62 316 367 212 50 457 36
Cuts at: 3608 2082
Size: 5018
Fragments arranged by size:
5018 457 367 316 212 62 50 36
26
PstI C_TGCA'G
Cuts at: 830 2358 2655 2725 3896 830
Size: 1528 297 70 1171 3478
Fragments arranged by size:
3478 1528 1171 297 70
PvuI CG_AT'CG
Cuts at: 2001 2758 2001
Size: 757 5787
PvuII CAG'CTG
Cuts at: 2135 2850 2917 3088 3438 3490 3672 2135
Size: 715 67 171 350 52 182 5007
Fragments arranged by size:
5007 715 350 182 171 67 52
SacI G_AGCT'C
Cuts at: 2701 3471 2701
Size: 770 5774
SacII CC_GC'GG
Cuts at: 3367 3367
Size: 6544
SalI G'TCGA_C
Cuts at: 1936 3886 1936
Size: 1950 4594
SanDI GG'GwC_CC
Cuts at: 3252 3252
Size: 6544
SbfI CC_TGCA'GG
Cuts at: 2358 3896 2358
Size: 1538 5006
ScaI AGT'ACT
Cuts at: 4792 6504 4792
Size: 1712 4832
SfcI C'TryA_G
Cuts at: 826 1045 2354 2422 2651 2721 3030 3667 3892
Size: 219 1309 68 229 70 309 637 225
Cuts at: 3892 5027 5708 5899 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1309 1135 681 637 309 229 225
219 191 70 68
SfiI GGCCn_nnn'nGGCC
Cuts at: 2882 2882
Size: 6544
SfoI GGC'GCC
Cuts at: 2011 2011
Size: 6544
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6544
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6544
SmaI CCC'GGG
Cuts at: 1872 2087 3871 1872
Size: 215 1784 4545
Fragments arranged by size:
4545 1784 215
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2339 4648 5549 5811 820
Size: 197 926 396 2309 901 262 1553
Fragments arranged by size:
2309 1553 926 901 396 262 197
SnaBI TAC'GTA
Cuts at: 493 4335 493
Size: 3842 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6544
SphI G_CATG'C
Cuts at: 1132 3662 3731 3902 1132
Size: 2530 69 171 3774
Fragments arranged by size:
3774 2530 171 69
SspI AAT'ATT
Cuts at: 5 52 4468 5
Size: 47 4416 2081
Fragments arranged by size:
4416 2081 47
StuI AGG'CCT
Cuts at: 1517 3196 1517
Size: 1679 4865
StyI C'CwwG_G
Cuts at: 513 1065 2004 2247 2967 3144 3799 3983 513
Size: 552 939 243 720 177 655 184 3074
Fragments arranged by size:
3074 939 720 655 552 243 184 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2768 3433 3485 3640 4571 4588 4741
Size: 1255 1188 665 52 155 931 17 153
Cuts at: 4741 4926 5371 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1188 931 665 445 185 155
153 52 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3298 4790 6502
Size: 274 80 33 51 1443 1321 1492 1712
Cuts at: 6502 96
Size: 138
Fragments arranged by size:
1712 1492 1443 1321 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2378 2410 2963 2995 3342 3374 6504 6536 2378
Size: 32 553 32 347 32 3130 32 2386
Fragments arranged by size:
3130 2386 553 347 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2905 1316
Size: 1589 4955
XbaI T'CTAG_A
Cuts at: 3880 3880
Size: 6544
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3151 3442 3494 3151
Size: 291 52 6201
Fragments arranged by size:
6201 291 52
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6544
XmaI C'CCGG_G
Cuts at: 1870 2085 3869 1870
Size: 215 1784 4545
Fragments arranged by size:
4545 1784 215
XmnI GAAnn'nnTTC
Cuts at: 4673 4673
Size: 6544
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4352 276
Size: 53 83 186 936 2818 2468
Fragments arranged by size:
2818 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbvCI BsiWI BsmBI BstZ17I DraIII
EcoRV FalI FseI FspAI NruI PacI PfoI PspXI
PsrI RsrII SapI SexAI SrfI SwaI