Restriction map for
pFN21AE2148
(Circular) (Six-base) MAPSORT of: pFN21AE2148.seq Check: 6948 from: 1 to: 5161
pFN21AE2148 5161 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2971 278
Size: 53 83 186 936 1435 2468
Fragments arranged by size:
2468 1435 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2504 1937
Size: 567 4594
Acc65I G'GTAC_C
Cuts at: 2482 2482
Size: 5161
AclI AA'CG_TT
Cuts at: 1563 3288 3661 1563
Size: 1725 373 3063
Fragments arranged by size:
3063 1725 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2366 3224 4608 4876 1632
Size: 734 858 1384 268 1917
Fragments arranged by size:
1917 1384 858 734 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4964
AflIII A'CryG_T
Cuts at: 1203 2200 4060 5037 1203
Size: 997 1860 977 1327
Fragments arranged by size:
1860 1327 997 977
AgeI A'CCGG_T
Cuts at: 3992 3992
Size: 5161
AhdI GACnn_n'nnGTC
Cuts at: 3890 3890
Size: 5161
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5161
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5161
AlwNI CAG_nnn'CTG
Cuts at: 2904 4476 2904
Size: 1572 3589
ApaI G_GGCC'C
Cuts at: 2362 2362
Size: 5161
ApaLI G'TGCA_C
Cuts at: 3218 4374 3218
Size: 1156 4005
ApoI r'AATT_y
Cuts at: 1493 2470 2696 2848 4957 1493
Size: 977 226 152 2109 1697
Fragments arranged by size:
2109 1697 977 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3715 160
Size: 872 2683 1606
Fragments arranged by size:
2683 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5161
AvaI C'yCGr_G
Cuts at: 1870 1943 2486 1870
Size: 73 543 4545
Fragments arranged by size:
4545 543 73
BamHI G'GATC_C
Cuts at: 2491 2858 2491
Size: 367 4794
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2482 3937 618
Size: 325 217 627 695 1455 1842
Fragments arranged by size:
1842 1455 695 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2362 2480 1391
Size: 971 118 4072
Fragments arranged by size:
4072 971 118
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2337 928
Size: 971 438 3752
Fragments arranged by size:
3752 971 438
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3352 3386 1903
Size: 34 1415 34 3678
Fragments arranged by size:
3678 1415 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3053 4263 3053
Size: 1210 3951
BclI T'GATC_A
Cuts at: 1548 2205 1548
Size: 657 4504
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2430 3772 136
Size: 107 122 71 631 605 758 1342 1525
Fragments arranged by size:
1525 1342 758 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5156 5156
Size: 5161
BlpI GC'TnA_GC
Cuts at: 2578 2578
Size: 5161
Bme1580I G_kGCm'C
Cuts at: 1790 2362 3222 4378 1790
Size: 572 860 1156 2573
Fragments arranged by size:
2573 1156 860 572
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5161
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2892 3850 442
Size: 416 53 83 399 24 1475 958 1753
Fragments arranged by size:
1753 1475 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2357 3821 1239
Size: 1118 1464 2579
Fragments arranged by size:
2579 1464 1118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3286 4151 4449 3286
Size: 865 298 3998
Fragments arranged by size:
3998 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3824 882
Size: 617 2325 2219
Fragments arranged by size:
2325 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2952 493
Size: 711 1748 2702
Fragments arranged by size:
2702 1748 711
BsaBI GATnn'nnATC
Cuts at: 5155 5155
Size: 5161
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2968 3350 3985 275
Size: 53 83 186 936 1435 382 635 1451
Fragments arranged by size:
1451 1435 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3594 3992 4266 4413 4824 3594
Size: 398 274 147 411 3931
Fragments arranged by size:
3931 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4936
Fragments arranged by size:
4936 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5161
BseYI C'CCAG_C
Cuts at: 4364 4860 4364
Size: 496 4665
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4588
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2611 3372 4400 1540
Size: 275 186 610 761 1028 2301
Fragments arranged by size:
2301 1028 761 610 275 186
BsiHKAI G_wGCw'C
Cuts at: 3222 3307 4378 3222
Size: 85 1071 4005
Fragments arranged by size:
4005 1071 85
BsmI GAATG_Cn'
Cuts at: 2672 2765 2672
Size: 93 5068
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2362 2480 3222 3307 4378 1391
Size: 399 572 118 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 572 399 118 85
BspEI T'CCGG_A
Cuts at: 4824 4824
Size: 5161
BspHI T'CATG_A
Cuts at: 3050 4780 3050
Size: 1730 3431
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2516 844
Size: 1672 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3048 1394
Size: 429 1225 3507
Fragments arranged by size:
3507 1225 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3656 3830 65
Size: 1183 2408 174 1396
Fragments arranged by size:
2408 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3805 3992 1700
Size: 110 1995 187 2869
Fragments arranged by size:
2869 1995 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5161
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5161
BssSI C'ACGA_G
Cuts at: 3221 4233 3221
Size: 1012 4149
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2041 794
Size: 1247 3914
BstBI TT'CG_AA
Cuts at: 3965 3965
Size: 5161
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5161
BstXI CCAn_nnnn'nTGG
Cuts at: 2311 2311
Size: 5161
BstYI r'GATC_y
Cuts at: 1972 2238 2491 2858 3243 3260 4712 4798 4810
Size: 266 253 367 385 17 1452 86 12
Cuts at: 4810 5156 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 266 253 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2428 2439 513
Size: 552 1363 11 3235
Fragments arranged by size:
3235 1363 552 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4422
Fragments arranged by size:
4422 683 56
BtsI GCAGTG_nn'
Cuts at: 2094 2677 3471 3491 2094
Size: 583 794 20 3764
Fragments arranged by size:
3764 794 583 20
ClaI AT'CG_AT
Cuts at: 1554 2064 1554
Size: 510 4651
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5126
DraI TTT'AAA
Cuts at: 2465 2465
Size: 5161
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4168 809
Size: 507 2852 1802
Fragments arranged by size:
2852 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2422 2431 2608
Size: 54 1425 50 275 18 592 9 177
Cuts at: 2608 3497 5126 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 592 275 177 54 50
43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2608 1537
Size: 275 796 4090
Fragments arranged by size:
4090 796 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3091 1353
Size: 1738 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2089 3748 4122 4268 387
Size: 1064 138 500 1659 374 146 1280
Fragments arranged by size:
1659 1280 1064 500 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2357 2366 3224 3821 4608 4876 1239
Size: 393 725 9 858 597 787 268 1524
Fragments arranged by size:
1524 858 787 725 597 393 268 9
EcoO109I rG'GnC_Cy
Cuts at: 2296 2386 2296
Size: 90 5071
EcoRI G'AATT_C
Cuts at: 2470 2848 2470
Size: 378 4783
FspI TGC'GCA
Cuts at: 3667 3667
Size: 5161
HaeII r_GCGC'y
Cuts at: 1678 4308 1678
Size: 2630 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3802 3834 3876 3908 1373
Size: 32 135 32 2230 32 42 32 2626
Fragments arranged by size:
2626 2230 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2505 2751 669
Size: 988 281 567 246 3079
Fragments arranged by size:
3079 988 567 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5161
HpaI GTT'AAC
Cuts at: 2751 2751
Size: 5161
KpnI G_GTAC'C
Cuts at: 2486 2486
Size: 5161
MfeI C'AATT_G
Cuts at: 2760 2760
Size: 5161
MluI A'CGCG_T
Cuts at: 5037 5037
Size: 5161
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4275 4459 1305
Size: 2970 184 2007
Fragments arranged by size:
2970 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3339
Fragments arranged by size:
3339 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2213 3119 3478 3637 518
Size: 648 1047 906 359 159 2042
Fragments arranged by size:
2042 1047 906 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2577 3254 4402 4647 1606
Size: 971 677 1148 245 2120
Fragments arranged by size:
2120 1148 971 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4609
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5161
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5161
NotI GC'GGCC_GC
Cuts at: 2608 2608
Size: 5161
NspI r_CATG'y
Cuts at: 1132 1229 2519 4064 1132
Size: 97 1290 1545 2229
Fragments arranged by size:
2229 1545 1290 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5161
PciI A'CATG_T
Cuts at: 4060 4060
Size: 5161
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5161
PmeI GTTT'AAAC
Cuts at: 2465 2465
Size: 5161
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3230 3262 4743 4775 3230
Size: 32 1481 32 3616
Fragments arranged by size:
3616 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2296 2296
Size: 5161
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5161
PsiI TTA'TAA
Cuts at: 2731 2731
Size: 5161
PspOMI G'GGCC_C
Cuts at: 2358 2358
Size: 5161
PstI C_TGCA'G
Cuts at: 830 2513 830
Size: 1683 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5161
SalI G'TCGA_C
Cuts at: 1936 2503 1936
Size: 567 4594
SbfI CC_TGCA'GG
Cuts at: 2513 2513
Size: 5161
ScaI AGT'ACT
Cuts at: 2049 3409 5121 2049
Size: 1360 1712 2089
Fragments arranged by size:
2089 1712 1360
SfcI C'TryA_G
Cuts at: 826 1045 2509 3644 4325 4516 826
Size: 219 1464 1135 681 191 1471
Fragments arranged by size:
1471 1464 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2430 2430
Size: 5161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5161
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5161
SmaI CCC'GGG
Cuts at: 1872 2488 1872
Size: 616 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3265 4166 4428 820
Size: 197 926 1322 901 262 1553
Fragments arranged by size:
1553 1322 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2952 493
Size: 2459 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5161
SphI G_CATG'C
Cuts at: 1132 2519 1132
Size: 1387 3774
SspI AAT'ATT
Cuts at: 5 52 3085 5
Size: 47 3033 2081
Fragments arranged by size:
3033 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5161
StyI C'CwwG_G
Cuts at: 513 1065 2600 513
Size: 552 1535 3074
Fragments arranged by size:
3074 1535 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2432 3188 3205 3358 3543 3988 325
Size: 1255 852 756 17 153 185 445 1498
Fragments arranged by size:
1498 1255 852 756 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2047 2232 3407
Size: 274 80 33 51 1443 70 185 1175
Cuts at: 3407 5119 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1175 274 185 138 80 70
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5121 5153 5121
Size: 32 5129
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5161
XbaI T'CTAG_A
Cuts at: 2497 2497
Size: 5161
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2107 2107
Size: 5161
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5161
XmaI C'CCGG_G
Cuts at: 1870 2486 1870
Size: 616 4545
XmnI GAAnn'nnTTC
Cuts at: 3290 3290
Size: 5161
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2969 276
Size: 53 83 186 936 1435 2468
Fragments arranged by size:
2468 1435 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfoI SrfI SwaI