Restriction map for
pFN21AE2147
(Circular) (Six-base) MAPSORT of: pFN21AE2147.seq Check: 4251 from: 1 to: 5077
pFN21AE2147 5077 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2887 278
Size: 53 83 186 936 1351 2468
Fragments arranged by size:
2468 1351 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2420 1937
Size: 483 4594
Acc65I G'GTAC_C
Cuts at: 2398 2398
Size: 5077
AclI AA'CG_TT
Cuts at: 1563 3204 3577 1563
Size: 1641 373 3063
Fragments arranged by size:
3063 1641 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3140 4524 4792 1632
Size: 1508 1384 268 1917
Fragments arranged by size:
1917 1508 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4880
AflIII A'CryG_T
Cuts at: 1203 3976 4953 1203
Size: 2773 977 1327
Fragments arranged by size:
2773 1327 977
AgeI A'CCGG_T
Cuts at: 2040 3908 2040
Size: 1868 3209
AhdI GACnn_n'nnGTC
Cuts at: 3806 3806
Size: 5077
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5077
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5077
AlwNI CAG_nnn'CTG
Cuts at: 2820 4392 2820
Size: 1572 3505
ApaLI G'TGCA_C
Cuts at: 3134 4290 3134
Size: 1156 3921
ApoI r'AATT_y
Cuts at: 1493 2163 2386 2612 2764 4873 1493
Size: 670 223 226 152 2109 1697
Fragments arranged by size:
2109 1697 670 226 223 152
AseI AT'TA_AT
Cuts at: 160 1032 3631 160
Size: 872 2599 1606
Fragments arranged by size:
2599 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5077
AvaI C'yCGr_G
Cuts at: 1870 1943 2250 2402 1870
Size: 73 307 152 4545
Fragments arranged by size:
4545 307 152 73
BamHI G'GATC_C
Cuts at: 2407 2774 2407
Size: 367 4710
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 2398 3853 618
Size: 325 217 627 288 323 1455 1842
Fragments arranged by size:
1842 1455 627 325 323 288 217
BanII G_rGCy'C
Cuts at: 1391 2284 2355 2396 1391
Size: 893 71 41 4072
Fragments arranged by size:
4072 893 71 41
BbeI G_GCGC'C
Cuts at: 2079 2079
Size: 5077
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3268 3302 1903
Size: 34 1331 34 3678
Fragments arranged by size:
3678 1331 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2969 4179 2969
Size: 1210 3867
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5077
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3688 136
Size: 107 122 71 631 605 337 1679 1525
Fragments arranged by size:
1679 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5072 5072
Size: 5077
BlpI GC'TnA_GC
Cuts at: 2494 2494
Size: 5077
Bme1580I G_kGCm'C
Cuts at: 1790 3138 4294 1790
Size: 1348 1156 2573
Fragments arranged by size:
2573 1348 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5077
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2808 3766 442
Size: 416 53 83 399 24 1391 958 1753
Fragments arranged by size:
1753 1391 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5077
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2339 3737 1239
Size: 1100 1398 2579
Fragments arranged by size:
2579 1398 1100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3202 4067 4365 3202
Size: 865 298 3914
Fragments arranged by size:
3914 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2104 3740 882
Size: 617 605 1636 2219
Fragments arranged by size:
2219 1636 617 605
BsaAI yAC'GTr
Cuts at: 493 1204 2868 493
Size: 711 1664 2702
Fragments arranged by size:
2702 1664 711
BsaBI GATnn'nnATC
Cuts at: 5071 5071
Size: 5077
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 2884 3266 3901
Size: 53 83 186 936 543 808 382 635
Cuts at: 3901 275
Size: 1451
Fragments arranged by size:
1451 936 808 635 543 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2040 3510 3908 4182 4329 4740 2040
Size: 1470 398 274 147 411 2377
Fragments arranged by size:
2377 1470 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4852
Fragments arranged by size:
4852 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5077
BseYI C'CCAG_C
Cuts at: 4280 4776 4280
Size: 496 4581
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4504
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2109 2527 3288 4316 1540
Size: 275 186 108 418 761 1028 2301
Fragments arranged by size:
2301 1028 761 418 275 186 108
BsiHKAI G_wGCw'C
Cuts at: 2284 3138 3223 4294 2284
Size: 854 85 1071 3067
Fragments arranged by size:
3067 1071 854 85
BsmI GAATG_Cn'
Cuts at: 2588 2681 2588
Size: 93 4984
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2284 2355 2396 3138 3223 4294 1391
Size: 399 494 71 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 494 399 85 71 41
BspEI T'CCGG_A
Cuts at: 4740 4740
Size: 5077
BspHI T'CATG_A
Cuts at: 2966 4696 2966
Size: 1730 3347
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2432 844
Size: 1588 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2964 1394
Size: 429 1141 3507
Fragments arranged by size:
3507 1141 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3572 3746 65
Size: 1183 2324 174 1396
Fragments arranged by size:
2324 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2028 2040 2330 3721 3908 1700
Size: 110 218 12 290 1391 187 2869
Fragments arranged by size:
2869 1391 290 218 187 110 12
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5077
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5077
BssSI C'ACGA_G
Cuts at: 3137 4149 3137
Size: 1012 4065
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5077
BstBI TT'CG_AA
Cuts at: 3881 3881
Size: 5077
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5077
BstYI r'GATC_y
Cuts at: 1972 2407 2774 3159 3176 4628 4714 4726 5072
Size: 435 367 385 17 1452 86 12 346
Cuts at: 5072 1972
Size: 1977
Fragments arranged by size:
1977 1452 435 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3586
Fragments arranged by size:
3586 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2030 2280 487
Size: 683 56 804 250 3284
Fragments arranged by size:
3284 804 683 250 56
BtsI GCAGTG_nn'
Cuts at: 2593 3387 3407 2593
Size: 794 20 4263
Fragments arranged by size:
4263 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5077
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5042
DraI TTT'AAA
Cuts at: 2381 2381
Size: 5077
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4084 809
Size: 507 2768 1802
Fragments arranged by size:
2768 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2131 2287 2524
Size: 54 1425 50 275 18 301 156 237
Cuts at: 2524 3413 5042 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 301 275 237 156 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2524 1537
Size: 275 712 4090
Fragments arranged by size:
4090 712 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3007 1353
Size: 1654 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3664 4038 4184 387
Size: 1064 138 2075 374 146 1280
Fragments arranged by size:
2075 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2282 2282
Size: 5077
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 3140 3737 4524 4792 1239
Size: 393 707 801 597 787 268 1524
Fragments arranged by size:
1524 801 787 707 597 393 268
EcoRI G'AATT_C
Cuts at: 2163 2386 2764 2163
Size: 223 378 4476
Fragments arranged by size:
4476 378 223
FspI TGC'GCA
Cuts at: 3583 3583
Size: 5077
HaeII r_GCGC'y
Cuts at: 1678 2079 4224 1678
Size: 401 2145 2531
Fragments arranged by size:
2531 2145 401
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3718 3750 3792 3824 1373
Size: 32 135 32 2146 32 42 32 2626
Fragments arranged by size:
2626 2146 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2421 2667 669
Size: 988 281 483 246 3079
Fragments arranged by size:
3079 988 483 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5077
HpaI GTT'AAC
Cuts at: 2667 2667
Size: 5077
KasI G'GCGC_C
Cuts at: 2075 2075
Size: 5077
KpnI G_GTAC'C
Cuts at: 2402 2402
Size: 5077
MfeI C'AATT_G
Cuts at: 2676 2676
Size: 5077
MluI A'CGCG_T
Cuts at: 4953 4953
Size: 5077
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4191 4375 1305
Size: 2886 184 2007
Fragments arranged by size:
2886 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2133 2289 10
Size: 54 1425 343 301 156 2798
Fragments arranged by size:
2798 1425 343 301 156 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3035 3394 3553 518
Size: 648 1869 359 159 2042
Fragments arranged by size:
2042 1869 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2493 3170 4318 4563 1606
Size: 887 677 1148 245 2120
Fragments arranged by size:
2120 1148 887 677 245
NaeI GCC'GGC
Cuts at: 2030 2030
Size: 5077
NarI GG'CG_CC
Cuts at: 2076 2076
Size: 5077
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3586
Fragments arranged by size:
3586 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5077
NgoMIV G'CCGG_C
Cuts at: 2028 2028
Size: 5077
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5077
NotI GC'GGCC_GC
Cuts at: 2524 2524
Size: 5077
NspI r_CATG'y
Cuts at: 1132 1229 2340 2435 3980 1132
Size: 97 1111 95 1545 2229
Fragments arranged by size:
2229 1545 1111 97 95
PasI CC'CwG_GG
Cuts at: 1348 2071 1348
Size: 723 4354
PciI A'CATG_T
Cuts at: 3976 3976
Size: 5077
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5077
PmeI GTTT'AAAC
Cuts at: 2381 2381
Size: 5077
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3146 3178 4659 4691 3146
Size: 32 1481 32 3532
Fragments arranged by size:
3532 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5077
PsiI TTA'TAA
Cuts at: 2647 2647
Size: 5077
PstI C_TGCA'G
Cuts at: 830 2429 830
Size: 1599 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5077
SacI G_AGCT'C
Cuts at: 2284 2284
Size: 5077
SalI G'TCGA_C
Cuts at: 1936 2419 1936
Size: 483 4594
SbfI CC_TGCA'GG
Cuts at: 2429 2429
Size: 5077
ScaI AGT'ACT
Cuts at: 3325 5037 3325
Size: 1712 3365
SfcI C'TryA_G
Cuts at: 826 1045 2155 2425 3560 4241 4432 826
Size: 219 1110 270 1135 681 191 1471
Fragments arranged by size:
1471 1135 1110 681 270 219 191
SfoI GGC'GCC
Cuts at: 2077 2077
Size: 5077
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5077
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5077
SmaI CCC'GGG
Cuts at: 1872 2404 1872
Size: 532 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3181 4082 4344 820
Size: 197 926 1238 901 262 1553
Fragments arranged by size:
1553 1238 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2868 493
Size: 2375 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5077
SphI G_CATG'C
Cuts at: 1132 2435 1132
Size: 1303 3774
SspI AAT'ATT
Cuts at: 5 52 3001 5
Size: 47 2949 2081
Fragments arranged by size:
2949 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5077
StyI C'CwwG_G
Cuts at: 513 1065 2004 2516 513
Size: 552 939 512 3074
Fragments arranged by size:
3074 939 552 512
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2307 3104 3121 3274 3459 3904 325
Size: 1255 727 797 17 153 185 445 1498
Fragments arranged by size:
1498 1255 797 727 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3323 5035 96
Size: 274 80 33 51 1443 1346 1712 138
Fragments arranged by size:
1712 1443 1346 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5037 5069 5037
Size: 32 5045
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5077
XbaI T'CTAG_A
Cuts at: 2413 2413
Size: 5077
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5077
XmaI C'CCGG_G
Cuts at: 1870 2402 1870
Size: 532 4545
XmnI GAAnn'nnTTC
Cuts at: 3206 3206
Size: 5077
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2885 276
Size: 53 83 186 936 1351 2468
Fragments arranged by size:
2468 1351 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI