Restriction map for
pFN21AE2134
(Circular) (Six-base) MAPSORT of: pFN21AE2134.seq Check: 3768 from: 1 to: 7279
pFN21AE2134 7279 bp
With 182 enzymes: SgfI *
December 8, 2014 13:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4326 4326
Size: 7279
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5089 278
Size: 53 83 186 936 3553 2468
Fragments arranged by size:
3553 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2246 4622 1937
Size: 309 2376 4594
Fragments arranged by size:
4594 2376 309
Acc65I G'GTAC_C
Cuts at: 3343 4600 3343
Size: 1257 6022
AclI AA'CG_TT
Cuts at: 1563 5406 5779 1563
Size: 3843 373 3063
Fragments arranged by size:
3843 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2260 3396 4084 4303 5342 6726 6994 1632
Size: 628 1136 688 219 1039 1384 268 1917
Fragments arranged by size:
1917 1384 1136 1039 688 628 268 219
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7082
AflIII A'CryG_T
Cuts at: 1203 6178 7155 1203
Size: 4975 977 1327
Fragments arranged by size:
4975 1327 977
AgeI A'CCGG_T
Cuts at: 6110 6110
Size: 7279
AhdI GACnn_n'nnGTC
Cuts at: 6008 6008
Size: 7279
AleI CACnn'nnGTG
Cuts at: 1166 4121 1166
Size: 2955 4324
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2358 1184
Size: 1174 6105
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3423 3455 3423
Size: 32 7247
AlwNI CAG_nnn'CTG
Cuts at: 2189 2227 2356 4033 4067 5022 6594 2189
Size: 38 129 1677 34 955 1572 2874
Fragments arranged by size:
2874 1677 1572 955 129 38 34
ApaLI G'TGCA_C
Cuts at: 5336 6492 5336
Size: 1156 6123
ApoI r'AATT_y
Cuts at: 1493 2064 2431 2512 3067 3146 4588 4814 4966
Size: 571 367 81 555 79 1442 226 152
Cuts at: 4966 7075 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1442 571 555 367 226 152
81 79
AseI AT'TA_AT
Cuts at: 160 1032 5833 160
Size: 872 4801 1606
Fragments arranged by size:
4801 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7279
AvaI C'yCGr_G
Cuts at: 1870 1943 2234 2573 4604 1870
Size: 73 291 339 2031 4545
Fragments arranged by size:
4545 2031 339 291 73
AvrII C'CTAG_G
Cuts at: 2029 2029
Size: 7279
BamHI G'GATC_C
Cuts at: 4609 4976 4609
Size: 367 6912
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2801 3343 4305 4600 6055
Size: 325 217 627 1014 542 962 295 1455
Cuts at: 6055 618
Size: 1842
Fragments arranged by size:
1842 1455 1014 962 627 542 325 295
217
BanII G_rGCy'C
Cuts at: 1391 2235 2574 2580 3483 4598 1391
Size: 844 339 6 903 1115 4072
Fragments arranged by size:
4072 1115 903 844 339 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2896 928
Size: 971 997 5311
Fragments arranged by size:
5311 997 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5470 5504 1903
Size: 34 3533 34 3678
Fragments arranged by size:
3678 3533 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5171 6381 5171
Size: 1210 6069
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7279
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5890 136
Size: 107 122 71 631 605 4218 1525
Fragments arranged by size:
4218 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7274 7274
Size: 7279
BlpI GC'TnA_GC
Cuts at: 2162 4696 2162
Size: 2534 4745
Bme1580I G_kGCm'C
Cuts at: 1790 4061 5340 6496 1790
Size: 2271 1279 1156 2573
Fragments arranged by size:
2573 2271 1279 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7279
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2659 4387 5010
Size: 416 53 83 399 24 1242 1728 623
Cuts at: 5010 5968 442
Size: 958 1753
Fragments arranged by size:
1753 1728 1242 958 623 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7279
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2057 2057
Size: 7279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2708 3338 4454 5939 1239
Size: 1010 459 630 1116 1485 2579
Fragments arranged by size:
2579 1485 1116 1010 630 459
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2921 3253 5404 6269 6567 2921
Size: 332 2151 865 298 3633
Fragments arranged by size:
3633 2151 865 332 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2004 2399 5942 882
Size: 617 505 395 3543 2219
Fragments arranged by size:
3543 2219 617 505 395
BsaAI yAC'GTr
Cuts at: 493 1204 5070 493
Size: 711 3866 2702
Fragments arranged by size:
3866 2702 711
BsaBI GATnn'nnATC
Cuts at: 7273 7273
Size: 7279
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5086 5468 6103 275
Size: 53 83 186 936 3553 382 635 1451
Fragments arranged by size:
3553 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5712 6110 6384 6531 6942 5712
Size: 398 274 147 411 6049
Fragments arranged by size:
6049 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3336 3366 1178
Size: 30 165 30 1933 30 5091
Fragments arranged by size:
5091 1933 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3376 1186
Size: 2190 5089
BseYI C'CCAG_C
Cuts at: 3422 3809 6482 6978 3422
Size: 387 2673 496 3723
Fragments arranged by size:
3723 2673 496 387
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4145 1163
Size: 573 2409 4297
Fragments arranged by size:
4297 2409 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4729 5490 6518 1540
Size: 275 186 2728 761 1028 2301
Fragments arranged by size:
2728 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2043 2235 2574 3483 4363 5340 5425 6496 2043
Size: 192 339 909 880 977 85 1071 2826
Fragments arranged by size:
2826 1071 977 909 880 339 192 85
BsmI GAATG_Cn'
Cuts at: 4135 4790 4883 4135
Size: 655 93 6531
Fragments arranged by size:
6531 655 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2235 2574 2580 3483 4061 4363
Size: 399 253 192 339 6 903 578 302
Cuts at: 4363 4598 5340 5425 6496 1391
Size: 235 742 85 1071 2174
Fragments arranged by size:
2174 1071 903 742 578 399 339 302
253 235 192 85 6
BspEI T'CCGG_A
Cuts at: 6942 6942
Size: 7279
BspHI T'CATG_A
Cuts at: 3966 5168 6898 3966
Size: 1202 1730 4347
Fragments arranged by size:
4347 1730 1202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2292 3652 4326 4634 844
Size: 1448 1360 674 308 3489
Fragments arranged by size:
3489 1448 1360 674 308
BsrBI CCG'CTC
Cuts at: 1394 1823 5166 1394
Size: 429 3343 3507
Fragments arranged by size:
3507 3343 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2752 3117 4224 5774 5948 65
Size: 1183 1504 365 1107 1550 174 1396
Fragments arranged by size:
1550 1504 1396 1183 1107 365 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 5923 6110 1700
Size: 110 238 3875 187 2869
Fragments arranged by size:
3875 2869 238 187 110
BsrGI T'GTAC_A
Cuts at: 96 4074 4432 96
Size: 3978 358 2943
Fragments arranged by size:
3978 2943 358
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7279
BssSI C'ACGA_G
Cuts at: 2919 5339 6351 2919
Size: 2420 1012 3847
Fragments arranged by size:
3847 2420 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7279
BstBI TT'CG_AA
Cuts at: 3065 6083 3065
Size: 3018 4261
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7279
BstXI CCAn_nnnn'nTGG
Cuts at: 4478 4478
Size: 7279
BstYI r'GATC_y
Cuts at: 1972 2455 2830 2884 3172 3657 4465 4609 4976
Size: 483 375 54 288 485 808 144 367
Cuts at: 4976 5361 5378 6830 6916 6928 7274 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 808 485 483 385 375 367
346 288 144 86 54 17 12
BstZ17I GTA'TAC
Cuts at: 2247 2247
Size: 7279
Bsu36I CC'TnA_GG
Cuts at: 3084 4461 3084
Size: 1377 5902
BtgI C'CryG_G
Cuts at: 513 1065 2004 3879 513
Size: 552 939 1875 3913
Fragments arranged by size:
3913 1875 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6540
Fragments arranged by size:
6540 683 56
BtsI GCAGTG_nn'
Cuts at: 2187 4795 5589 5609 2187
Size: 2608 794 20 3857
Fragments arranged by size:
3857 2608 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7279
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7244
DraI TTT'AAA
Cuts at: 2436 3369 4583 2436
Size: 933 1214 5132
Fragments arranged by size:
5132 1214 933
DraIII CAC_nnn'GTG
Cuts at: 3719 3719
Size: 7279
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6286 809
Size: 507 4970 1802
Fragments arranged by size:
4970 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2086 2859
Size: 54 1425 50 275 18 216 40 773
Cuts at: 2859 4726 5615 7244 8
Size: 1867 889 1629 43
Fragments arranged by size:
1867 1629 1425 889 773 275 216 54
50 43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 4726 1537
Size: 275 2914 4090
Fragments arranged by size:
4090 2914 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3872 3891 5209 1353
Size: 2519 19 1318 3423
Fragments arranged by size:
3423 2519 1318 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5866 6240 6386 387
Size: 1064 138 4277 374 146 1280
Fragments arranged by size:
4277 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2233 2572 3481 2233
Size: 339 909 6031
Fragments arranged by size:
6031 909 339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2260 2708 3338 3396 4084 4303
Size: 393 617 11 448 630 58 688 219
Cuts at: 4303 4454 5342 5939 6726 6994 1239
Size: 151 888 597 787 268 1524
Fragments arranged by size:
1524 888 787 688 630 617 597 448
393 268 219 151 58 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2120 2120
Size: 7279
EcoO109I rG'GnC_Cy
Cuts at: 2125 2134 4383 2125
Size: 9 2249 5021
Fragments arranged by size:
5021 2249 9
EcoRI G'AATT_C
Cuts at: 2064 2512 4588 4966 2064
Size: 448 2076 378 4377
Fragments arranged by size:
4377 2076 448 378
EcoRV GAT'ATC
Cuts at: 3226 3226
Size: 7279
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2290 2344 2290
Size: 54 7225
FspI TGC'GCA
Cuts at: 5785 5785
Size: 7279
HaeII r_GCGC'y
Cuts at: 1678 6426 1678
Size: 4748 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2522 2554 3157 3189 3585
Size: 32 135 32 950 32 603 32 396
Cuts at: 3585 3617 3836 3868 4191 4223 4255 4287 4396
Size: 32 219 32 323 32 32 32 109
Cuts at: 4396 4428 5920 5952 5994 6026 1373
Size: 32 1492 32 42 32 2626
Fragments arranged by size:
2626 1492 950 603 396 323 219 135
109 42 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4623 4869 669
Size: 988 281 2685 246 3079
Fragments arranged by size:
3079 2685 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7279
HpaI GTT'AAC
Cuts at: 4869 4869
Size: 7279
KpnI G_GTAC'C
Cuts at: 3347 4604 3347
Size: 1257 6022
MfeI C'AATT_G
Cuts at: 4878 4878
Size: 7279
MluI A'CGCG_T
Cuts at: 7155 7155
Size: 7279
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2565 3633 6393 6577 1305
Size: 1260 1068 2760 184 2007
Fragments arranged by size:
2760 2007 1260 1068 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2861 10
Size: 54 1425 343 1029 4428
Fragments arranged by size:
4428 1425 1029 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2340 3795 4121 4370 4373 5237 5596
Size: 648 1174 1455 326 249 3 864 359
Cuts at: 5596 5755 518
Size: 159 2042
Fragments arranged by size:
2042 1455 1174 864 648 359 326 249
159 3
MspA1I CmG'CkG
Cuts at: 1606 2218 2224 4695 5372 6520 6765 1606
Size: 612 6 2471 677 1148 245 2120
Fragments arranged by size:
2471 2120 1148 677 612 245 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5788
Fragments arranged by size:
5788 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7279
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7279
NotI GC'GGCC_GC
Cuts at: 4726 4726
Size: 7279
NspI r_CATG'y
Cuts at: 1132 1229 4637 6182 1132
Size: 97 3408 1545 2229
Fragments arranged by size:
3408 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4054 4330 1348
Size: 2706 276 4297
Fragments arranged by size:
4297 2706 276
PciI A'CATG_T
Cuts at: 6178 6178
Size: 7279
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2797 4033 1108
Size: 1689 1236 4354
Fragments arranged by size:
4354 1689 1236
PfoI T'CCnGG_A
Cuts at: 3150 3660 3671 4236 4470 3150
Size: 510 11 565 234 5959
Fragments arranged by size:
5959 565 510 234 11
PmeI GTTT'AAAC
Cuts at: 4583 4583
Size: 7279
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2556 2588 5348 5380 6861 6893 2556
Size: 32 2760 32 1481 32 2942
Fragments arranged by size:
2942 2760 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2134 4383 2125
Size: 9 2249 5021
Fragments arranged by size:
5021 2249 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7279
PsiI TTA'TAA
Cuts at: 4017 4849 4017
Size: 832 6447
PspXI vC'TCGA_Gb
Cuts at: 2573 2573
Size: 7279
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2948 2980 2948
Size: 32 7247
PstI C_TGCA'G
Cuts at: 830 2223 3823 4631 830
Size: 1393 1600 808 3478
Fragments arranged by size:
3478 1600 1393 808
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7279
PvuII CAG'CTG
Cuts at: 2218 2224 2218
Size: 6 7273
SacI G_AGCT'C
Cuts at: 2235 2574 3483 2235
Size: 339 909 6031
Fragments arranged by size:
6031 909 339
SalI G'TCGA_C
Cuts at: 1936 4621 1936
Size: 2685 4594
SbfI CC_TGCA'GG
Cuts at: 4631 4631
Size: 7279
ScaI AGT'ACT
Cuts at: 5527 7239 5527
Size: 1712 5567
SfcI C'TryA_G
Cuts at: 826 1045 2219 3391 3804 3819 4563 4627 5762
Size: 219 1174 1172 413 15 744 64 1135
Cuts at: 5762 6443 6634 826
Size: 681 191 1471
Fragments arranged by size:
1471 1174 1172 1135 744 681 413 219
191 64 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7279
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7279
SmaI CCC'GGG
Cuts at: 1872 4606 1872
Size: 2734 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2573 2936 3232 5383 6284 6546
Size: 197 926 630 363 296 2151 901 262
Cuts at: 6546 820
Size: 1553
Fragments arranged by size:
2151 1553 926 901 630 363 296 262
197
SnaBI TAC'GTA
Cuts at: 493 5070 493
Size: 4577 2702
SpeI A'CTAG_T
Cuts at: 152 3247 152
Size: 3095 4184
SphI G_CATG'C
Cuts at: 1132 4637 1132
Size: 3505 3774
SspI AAT'ATT
Cuts at: 5 52 2550 5203 5
Size: 47 2498 2653 2081
Fragments arranged by size:
2653 2498 2081 47
StuI AGG'CCT
Cuts at: 1517 2273 2768 1517
Size: 756 495 6028
Fragments arranged by size:
6028 756 495
StyI C'CwwG_G
Cuts at: 513 1065 2004 2029 3168 3442 4718 513
Size: 552 939 25 1139 274 1276 3074
Fragments arranged by size:
3074 1276 1139 939 552 274 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2135 2789 4111 5306 5323 5476 5661
Size: 1255 555 654 1322 1195 17 153 185
Cuts at: 5661 6106 325
Size: 445 1498
Fragments arranged by size:
1498 1322 1255 1195 654 555 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4074 4432 5525
Size: 274 80 33 51 1443 2097 358 1093
Cuts at: 5525 7237 96
Size: 1712 138
Fragments arranged by size:
2097 1712 1443 1093 358 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7239 7271 7239
Size: 32 7247
Tth111I GACn'n_nGTC
Cuts at: 1316 4205 1316
Size: 2889 4390
XbaI T'CTAG_A
Cuts at: 3316 4615 3316
Size: 1299 5980
XhoI C'TCGA_G
Cuts at: 1943 2573 1943
Size: 630 6649
XmaI C'CCGG_G
Cuts at: 1870 4604 1870
Size: 2734 4545
XmnI GAAnn'nnTTC
Cuts at: 2068 2669 5408 2068
Size: 601 2739 3939
Fragments arranged by size:
3939 2739 601
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5087 276
Size: 53 83 186 936 3553 2468
Fragments arranged by size:
3553 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI