Restriction map for
pFN21AE2122
(Circular) (Six-base) MAPSORT of: pFN21AE2122.seq Check: 3032 from: 1 to: 7420
pFN21AE2122 7420 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4051 4051
Size: 7420
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3456 5230 278
Size: 53 83 186 936 1920 1774 2468
Fragments arranged by size:
2468 1920 1774 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4763 1937
Size: 2826 4594
Acc65I G'GTAC_C
Cuts at: 4741 4741
Size: 7420
AclI AA'CG_TT
Cuts at: 1563 3801 5547 5920 1563
Size: 2238 1746 373 3063
Fragments arranged by size:
3063 2238 1746 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2615 2663 4647 5483 6867 7135 1632
Size: 983 48 1984 836 1384 268 1917
Fragments arranged by size:
1984 1917 1384 983 836 268 48
AfeI AGC'GCT
Cuts at: 2949 2949
Size: 7420
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7223
AflIII A'CryG_T
Cuts at: 1203 6319 7296 1203
Size: 5116 977 1327
Fragments arranged by size:
5116 1327 977
AgeI A'CCGG_T
Cuts at: 6251 6251
Size: 7420
AhdI GACnn_n'nnGTC
Cuts at: 6149 6149
Size: 7420
AleI CACnn'nnGTG
Cuts at: 1166 3518 1166
Size: 2352 5068
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2535 3032 1184
Size: 1351 497 5572
Fragments arranged by size:
5572 1351 497
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 7388
AlwNI CAG_nnn'CTG
Cuts at: 2593 3037 4408 4553 5163 6735 2593
Size: 444 1371 145 610 1572 3278
Fragments arranged by size:
3278 1572 1371 610 444 145
ApaLI G'TGCA_C
Cuts at: 5477 6633 5477
Size: 1156 6264
ApoI r'AATT_y
Cuts at: 1493 2212 4631 4697 4729 4955 5107 7216 1493
Size: 719 2419 66 32 226 152 2109 1697
Fragments arranged by size:
2419 2109 1697 719 226 152 66 32
AseI AT'TA_AT
Cuts at: 160 1032 4230 5974 160
Size: 872 3198 1744 1606
Fragments arranged by size:
3198 1744 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7420
AvaI C'yCGr_G
Cuts at: 1870 1943 2631 3648 4745 1870
Size: 73 688 1017 1097 4545
Fragments arranged by size:
4545 1097 1017 688 73
BamHI G'GATC_C
Cuts at: 2599 4750 5117 2599
Size: 2151 367 4902
Fragments arranged by size:
4902 2151 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2366 4741 6196 618
Size: 325 217 627 579 2375 1455 1842
Fragments arranged by size:
2375 1842 1455 627 579 325 217
BanII G_rGCy'C
Cuts at: 1391 2641 4422 4739 1391
Size: 1250 1781 317 4072
Fragments arranged by size:
4072 1781 1250 317
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2587 3448 928
Size: 971 688 861 4900
Fragments arranged by size:
4900 971 861 688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2816 2850 5611 5645 1903
Size: 34 879 34 2761 34 3678
Fragments arranged by size:
3678 2761 879 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5312 6522 5312
Size: 1210 6210
BclI T'GATC_A
Cuts at: 1548 3891 4164 4294 1548
Size: 2343 273 130 4674
Fragments arranged by size:
4674 2343 273 130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2636 2817 3305
Size: 107 122 71 631 605 964 181 488
Cuts at: 3305 4019 6031 136
Size: 714 2012 1525
Fragments arranged by size:
2012 1525 964 714 631 605 488 181
122 107 71
BglII A'GATC_T
Cuts at: 2254 3127 7415 2254
Size: 873 4288 2259
Fragments arranged by size:
4288 2259 873
BlpI GC'TnA_GC
Cuts at: 3041 4056 4837 3041
Size: 1015 781 5624
Fragments arranged by size:
5624 1015 781
Bme1580I G_kGCm'C
Cuts at: 1790 5481 6637 1790
Size: 3691 1156 2573
Fragments arranged by size:
3691 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7420
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5151 6109 442
Size: 416 53 83 399 24 3734 958 1753
Fragments arranged by size:
3734 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7420
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3104 4436 3104
Size: 1332 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2933 2945 3014 4575 6080 1239
Size: 1694 12 69 1561 1505 2579
Fragments arranged by size:
2579 1694 1561 1505 69 12
Bpu10I CC'TnA_GC
Cuts at: 2241 3438 2241
Size: 1197 6223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2661 2763 5545 6410 6708 2661
Size: 102 2782 865 298 3373
Fragments arranged by size:
3373 2782 865 298 102
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6083 882
Size: 617 4584 2219
Fragments arranged by size:
4584 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5211 493
Size: 711 4007 2702
Fragments arranged by size:
4007 2702 711
BsaBI GATnn'nnATC
Cuts at: 2315 7414 2315
Size: 5099 2321
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3453 5227 5609 6244
Size: 53 83 186 936 1920 1774 382 635
Cuts at: 6244 275
Size: 1451
Fragments arranged by size:
1920 1774 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2061 3615 5853 6251 6525 6672 7083 2061
Size: 1554 2238 398 274 147 411 2398
Fragments arranged by size:
2398 2238 1554 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3812 3842 1178
Size: 30 165 30 2409 30 4756
Fragments arranged by size:
4756 2409 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2314 2930 2953 3833 4052 4428 1186
Size: 1128 616 23 880 219 376 4178
Fragments arranged by size:
4178 1128 880 616 376 219 23
BseYI C'CCAG_C
Cuts at: 3692 6623 7119 3692
Size: 2931 496 3993
Fragments arranged by size:
3993 2931 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2953 4421 1163
Size: 573 1217 1468 4162
Fragments arranged by size:
4162 1468 1217 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2906 2983 4870 5631 6659 1540
Size: 275 186 905 77 1887 761 1028 2301
Fragments arranged by size:
2301 1887 1028 905 761 275 186 77
BsiHKAI G_wGCw'C
Cuts at: 4422 5481 5566 6637 4422
Size: 1059 85 1071 5205
Fragments arranged by size:
5205 1071 1059 85
BsmI GAATG_Cn'
Cuts at: 2656 4486 4931 5024 2656
Size: 1830 445 93 5052
Fragments arranged by size:
5052 1830 445 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2300 2970 2300
Size: 670 6750
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2641 4422 4739 5481 5566 6637 1391
Size: 399 851 1781 317 742 85 1071 2174
Fragments arranged by size:
2174 1781 1071 851 742 399 317 85
BspEI T'CCGG_A
Cuts at: 2061 7083 2061
Size: 5022 2398
BspHI T'CATG_A
Cuts at: 2442 4167 5309 7039 2442
Size: 1725 1142 1730 2823
Fragments arranged by size:
2823 1730 1725 1142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3045 3469 4051 4247 4775 844
Size: 2201 424 582 196 528 3489
Fragments arranged by size:
3489 2201 582 528 424 196
BsrBI CCG'CTC
Cuts at: 1394 1823 3228 3401 5307 1394
Size: 429 1405 173 1906 3507
Fragments arranged by size:
3507 1906 1405 429 173
BsrDI GCAATG_nn'
Cuts at: 65 1248 3093 3990 4135 4713 5915 6089 65
Size: 1183 1845 897 145 578 1202 174 1396
Fragments arranged by size:
1845 1396 1202 1183 897 578 174 145
BsrFI r'CCGG_y
Cuts at: 1700 1810 2759 2982 6064 6251 1700
Size: 110 949 223 3082 187 2869
Fragments arranged by size:
3082 2869 949 223 187 110
BsrGI T'GTAC_A
Cuts at: 96 2664 4681 96
Size: 2568 2017 2835
Fragments arranged by size:
2835 2568 2017
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7420
BssSI C'ACGA_G
Cuts at: 4281 4641 5480 6492 4281
Size: 360 839 1012 5209
Fragments arranged by size:
5209 1012 839 360
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7420
BstBI TT'CG_AA
Cuts at: 6224 6224
Size: 7420
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7420
BstXI CCAn_nnnn'nTGG
Cuts at: 2053 3286 2053
Size: 1233 6187
BstYI r'GATC_y
Cuts at: 1972 2254 2599 3082 3127 4750 5117 5502 5519
Size: 282 345 483 45 1623 367 385 17
Cuts at: 5519 6971 7057 7069 7415 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1623 1452 483 385 367 346 345
282 86 45 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2370 2400 3501 513
Size: 552 1305 30 1101 4432
Fragments arranged by size:
4432 1305 1101 552 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2740 3612 4434 487
Size: 683 56 1514 872 822 3473
Fragments arranged by size:
3473 1514 872 822 683 56
BtsI GCAGTG_nn'
Cuts at: 3159 4936 5730 5750 3159
Size: 1777 794 20 4829
Fragments arranged by size:
4829 1777 794 20
ClaI AT'CG_AT
Cuts at: 1554 3834 1554
Size: 2280 5140
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3349 3384 529
Size: 35 2785 35 4565
Fragments arranged by size:
4565 2785 35 35
DraI TTT'AAA
Cuts at: 2533 4724 2533
Size: 2191 5229
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6427 809
Size: 507 5111 1802
Fragments arranged by size:
5111 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2903 2955 3419
Size: 54 1425 50 275 18 1073 52 464
Cuts at: 3419 4107 4146 4867 5756 7385 8
Size: 688 39 721 889 1629 43
Fragments arranged by size:
1629 1425 1073 889 721 688 464 275
54 52 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2903 4867 1537
Size: 275 1091 1964 4090
Fragments arranged by size:
4090 1964 1091 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2259 2469 4607 5350 1353
Size: 906 210 2138 743 3423
Fragments arranged by size:
3423 2138 906 743 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2441 2678 3017 6007 6381 6527
Size: 1064 138 852 237 339 2990 374 146
Cuts at: 6527 387
Size: 1280
Fragments arranged by size:
2990 1280 1064 852 374 339 237 146
138
EcoICRI GAG'CTC
Cuts at: 4420 4420
Size: 7420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2615 2663 2933 2945 3014 4575 4647
Size: 393 983 48 270 12 69 1561 72
Cuts at: 4647 5483 6080 6867 7135 1239
Size: 836 597 787 268 1524
Fragments arranged by size:
1561 1524 983 836 787 597 393 270
268 72 69 48 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2222 3590 2222
Size: 1368 6052
EcoO109I rG'GnC_Cy
Cuts at: 2041 2479 3034 3897 2041
Size: 438 555 863 5564
Fragments arranged by size:
5564 863 555 438
EcoRI G'AATT_C
Cuts at: 4631 4697 4729 5107 4631
Size: 66 32 378 6944
Fragments arranged by size:
6944 378 66 32
EcoRV GAT'ATC
Cuts at: 4135 4135
Size: 7420
FspI TGC'GCA
Cuts at: 2294 5926 2294
Size: 3632 3788
HaeII r_GCGC'y
Cuts at: 1678 2951 3029 6567 1678
Size: 1273 78 3538 2531
Fragments arranged by size:
3538 2531 1273 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2239 2271 2333 2365 3152
Size: 32 135 32 667 32 62 32 787
Cuts at: 3152 3184 3500 3518 3532 3550 4060 4092 4573
Size: 32 316 18 14 18 510 32 481
Cuts at: 4573 4605 6061 6093 6135 6167 1373
Size: 32 1456 32 42 32 2626
Fragments arranged by size:
2626 1456 787 667 510 481 316 135
62 42 32 32 32 32 32 32
32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2122 3492 4558 4764 5010 669
Size: 988 281 184 1370 1066 206 246 3079
Fragments arranged by size:
3079 1370 1066 988 281 246 206 184
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7420
HpaI GTT'AAC
Cuts at: 5010 5010
Size: 7420
KpnI G_GTAC'C
Cuts at: 4745 4745
Size: 7420
MfeI C'AATT_G
Cuts at: 5019 5019
Size: 7420
MluI A'CGCG_T
Cuts at: 7296 7296
Size: 7420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2067 3345 3696 6534 6718 1305
Size: 762 1278 351 2838 184 2007
Fragments arranged by size:
2838 2007 1278 762 351 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4109 4148 10
Size: 54 1425 343 2277 39 3282
Fragments arranged by size:
3282 2277 1425 343 54 39
MslI CAynn'nnrTG
Cuts at: 518 1166 2697 3518 5378 5737 5896 518
Size: 648 1531 821 1860 359 159 2042
Fragments arranged by size:
2042 1860 1531 821 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2402 2940 4125 4553 4836 5513 6661 6906
Size: 796 538 1185 428 283 677 1148 245
Cuts at: 6906 1606
Size: 2120
Fragments arranged by size:
2120 1185 1148 796 677 538 428 283
245
NaeI GCC'GGC
Cuts at: 2761 2761
Size: 7420
NcoI C'CATG_G
Cuts at: 513 1065 2370 513
Size: 552 1305 5563
Fragments arranged by size:
5563 1305 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7420
NgoMIV G'CCGG_C
Cuts at: 2759 2759
Size: 7420
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7420
NotI GC'GGCC_GC
Cuts at: 4867 4867
Size: 7420
NspI r_CATG'y
Cuts at: 1132 1229 3278 4317 4778 6323 1132
Size: 97 2049 1039 461 1545 2229
Fragments arranged by size:
2229 2049 1545 1039 461 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7420
PciI A'CATG_T
Cuts at: 6319 6319
Size: 7420
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7420
PfoI T'CCnGG_A
Cuts at: 2217 2745 2868 3030 3180 2217
Size: 528 123 162 150 6457
Fragments arranged by size:
6457 528 162 150 123
PmeI GTTT'AAAC
Cuts at: 4724 4724
Size: 7420
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2882 2914 3812 3844 5489 5521 7002 7034 2882
Size: 32 898 32 1645 32 1481 32 3268
Fragments arranged by size:
3268 1645 1481 898 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2041 3034 3897 2041
Size: 993 863 5564
Fragments arranged by size:
5564 993 863
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7420
PsiI TTA'TAA
Cuts at: 4990 4990
Size: 7420
PspXI vC'TCGA_Gb
Cuts at: 3648 3648
Size: 7420
PstI C_TGCA'G
Cuts at: 830 3625 4382 4772 830
Size: 2795 757 390 3478
Fragments arranged by size:
3478 2795 757 390
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7420
PvuII CAG'CTG
Cuts at: 4553 4553
Size: 7420
SacI G_AGCT'C
Cuts at: 4422 4422
Size: 7420
SacII CC_GC'GG
Cuts at: 2403 2403
Size: 7420
SalI G'TCGA_C
Cuts at: 1936 4762 1936
Size: 2826 4594
SanDI GG'GwC_CC
Cuts at: 2041 2041
Size: 7420
SbfI CC_TGCA'GG
Cuts at: 4772 4772
Size: 7420
ScaI AGT'ACT
Cuts at: 5668 7380 5668
Size: 1712 5708
SfcI C'TryA_G
Cuts at: 826 1045 2193 2280 3621 4378 4768 5903 6584
Size: 219 1148 87 1341 757 390 1135 681
Cuts at: 6584 6775 826
Size: 191 1471
Fragments arranged by size:
1471 1341 1148 1135 757 681 390 219
191 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7420
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7420
SmaI CCC'GGG
Cuts at: 1872 4747 1872
Size: 2875 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2676 2778 3648 5524 6425 6687
Size: 197 926 733 102 870 1876 901 262
Cuts at: 6687 820
Size: 1553
Fragments arranged by size:
1876 1553 926 901 870 733 262 197
102
SnaBI TAC'GTA
Cuts at: 493 5211 493
Size: 4718 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7420
SphI G_CATG'C
Cuts at: 1132 3278 4778 1132
Size: 2146 1500 3774
Fragments arranged by size:
3774 2146 1500
SspI AAT'ATT
Cuts at: 5 52 5344 5
Size: 47 5292 2081
Fragments arranged by size:
5292 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7420
StyI C'CwwG_G
Cuts at: 513 1065 2370 2805 4859 513
Size: 552 1305 435 2054 3074
Fragments arranged by size:
3074 2054 1305 552 435
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2709 3377 5447 5464 5617 5802 6247
Size: 1255 1129 668 2070 17 153 185 445
Cuts at: 6247 325
Size: 1498
Fragments arranged by size:
2070 1498 1255 1129 668 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2179 2664 3048
Size: 274 80 33 51 1443 202 485 384
Cuts at: 3048 4141 4681 5666 7378 96
Size: 1093 540 985 1712 138
Fragments arranged by size:
1712 1443 1093 985 540 485 384 274
202 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7380 7412 7380
Size: 32 7388
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7420
XbaI T'CTAG_A
Cuts at: 4756 4756
Size: 7420
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4538 4538
Size: 7420
XhoI C'TCGA_G
Cuts at: 1943 3648 1943
Size: 1705 5715
XmaI C'CCGG_G
Cuts at: 1870 4745 1870
Size: 2875 4545
XmnI GAAnn'nnTTC
Cuts at: 3827 5549 3827
Size: 1722 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3454 5228 276
Size: 53 83 186 936 1920 1774 2468
Fragments arranged by size:
2468 1920 1774 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspOMI PsrI RsrII SapI
SexAI SfiI SfoI SrfI SwaI