Restriction map for
pFN21AE2114
(Circular) (Six-base) MAPSORT of: pFN21AE2114.seq Check: 1915 from: 1 to: 7351
pFN21AE2114 7351 bp
With 182 enzymes: SgfI *
September 11, 2017 14:08 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5161 278
Size: 53 83 186 936 3625 2468
Fragments arranged by size:
3625 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2191 2304 3026 3240 4694 1937
Size: 254 113 722 214 1454 4594
Fragments arranged by size:
4594 1454 722 254 214 113
Acc65I G'GTAC_C
Cuts at: 3382 4672 3382
Size: 1290 6061
AclI AA'CG_TT
Cuts at: 1563 4257 5478 5851 1563
Size: 2694 1221 373 3063
Fragments arranged by size:
3063 2694 1221 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2666 4365 5414 6798 7066 1632
Size: 1034 1699 1049 1384 268 1917
Fragments arranged by size:
1917 1699 1384 1049 1034 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7154
AflIII A'CryG_T
Cuts at: 1203 2115 3361 4626 6250 7227 1203
Size: 912 1246 1265 1624 977 1327
Fragments arranged by size:
1624 1327 1265 1246 977 912
AgeI A'CCGG_T
Cuts at: 2186 6182 2186
Size: 3996 3355
AhdI GACnn_n'nnGTC
Cuts at: 3272 6080 3272
Size: 2808 4543
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4143 1184
Size: 2959 4392
AlwNI CAG_nnn'CTG
Cuts at: 3101 5094 6666 3101
Size: 1993 1572 3786
Fragments arranged by size:
3786 1993 1572
ApaI G_GGCC'C
Cuts at: 2012 2012
Size: 7351
ApaLI G'TGCA_C
Cuts at: 5408 6564 5408
Size: 1156 6195
ApoI r'AATT_y
Cuts at: 1493 2170 2786 4122 4152 4429 4660 4886 5038
Size: 677 616 1336 30 277 231 226 152
Cuts at: 5038 7147 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1336 677 616 277 231 226
152 30
AseI AT'TA_AT
Cuts at: 160 1032 3609 5905 160
Size: 872 2577 2296 1606
Fragments arranged by size:
2577 2296 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7351
AvaI C'yCGr_G
Cuts at: 1870 1943 3636 4248 4676 1870
Size: 73 1693 612 428 4545
Fragments arranged by size:
4545 1693 612 428 73
BamHI G'GATC_C
Cuts at: 2734 4681 5048 2734
Size: 1947 367 5037
Fragments arranged by size:
5037 1947 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2125 2133 2364 3154 3382
Size: 325 217 627 338 8 231 790 228
Cuts at: 3382 3623 4672 6127 618
Size: 241 1049 1455 1842
Fragments arranged by size:
1842 1455 1049 790 627 338 325 241
231 228 217 8
BanII G_rGCy'C
Cuts at: 1391 2012 2085 2937 4224 4544 4670 1391
Size: 621 73 852 1287 320 126 4072
Fragments arranged by size:
4072 1287 852 621 320 126 73
BbeI G_GCGC'C
Cuts at: 2137 2137
Size: 7351
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2461 928
Size: 971 562 5818
Fragments arranged by size:
5818 971 562
BbvCI CC'TCA_GC
Cuts at: 2918 2918
Size: 7351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5542 5576 1903
Size: 34 3605 34 3678
Fragments arranged by size:
3678 3605 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5243 6453 5243
Size: 1210 6141
BclI T'GATC_A
Cuts at: 1548 2751 3090 1548
Size: 1203 339 5809
Fragments arranged by size:
5809 1203 339
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2094 5962 136
Size: 107 122 71 631 605 422 3868 1525
Fragments arranged by size:
3868 1525 631 605 422 122 107 71
BglII A'GATC_T
Cuts at: 7346 7346
Size: 7351
BlpI GC'TnA_GC
Cuts at: 3642 4768 3642
Size: 1126 6225
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2130 2240 2367 5412 6568 1790
Size: 222 118 110 127 3045 1156 2573
Fragments arranged by size:
3045 2573 1156 222 127 118 110
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7351
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2479 3006 5082
Size: 416 53 83 399 24 1062 527 2076
Cuts at: 5082 6040 442
Size: 958 1753
Fragments arranged by size:
2076 1753 1062 958 527 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2162 3623 3705 6011 1239
Size: 923 1461 82 2306 2579
Fragments arranged by size:
2579 2306 1461 923 82
Bpu10I CC'TnA_GC
Cuts at: 2918 2931 2918
Size: 13 7338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3989 4330 5476 6341 6639 3989
Size: 341 1146 865 298 4701
Fragments arranged by size:
4701 1146 865 341 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2059 3400 6014 882
Size: 617 560 1341 2614 2219
Fragments arranged by size:
2614 2219 1341 617 560
BsaAI yAC'GTr
Cuts at: 493 1204 3024 3141 3364 3670 3684 5142 493
Size: 711 1820 117 223 306 14 1458 2702
Fragments arranged by size:
2702 1820 1458 711 306 223 117 14
BsaBI GATnn'nnATC
Cuts at: 2750 4443 7345 2750
Size: 1693 2902 2756
Fragments arranged by size:
2902 2756 1693
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 2134 4083 5158
Size: 53 83 186 936 570 31 1949 1075
Cuts at: 5158 5540 6175 275
Size: 382 635 1451
Fragments arranged by size:
1949 1451 1075 936 635 570 382 186
83 53 31
BsaWI w'CCGG_w
Cuts at: 2186 3613 5784 6182 6456 6603 7014 2186
Size: 1427 2171 398 274 147 411 2523
Fragments arranged by size:
2523 2171 1427 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7126
Fragments arranged by size:
7126 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2047 2428 4216 4543 1186
Size: 861 381 1788 327 3994
Fragments arranged by size:
3994 1788 861 381 327
BseYI C'CCAG_C
Cuts at: 3223 6554 7050 3223
Size: 3331 496 3524
Fragments arranged by size:
3524 3331 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4304 1163
Size: 573 2568 4210
Fragments arranged by size:
4210 2568 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2191 4801 5562 6590 1540
Size: 275 186 190 2610 761 1028 2301
Fragments arranged by size:
2610 2301 1028 761 275 190 186
BsiHKAI G_wGCw'C
Cuts at: 2830 2937 3669 4544 5412 5497 6568 2830
Size: 107 732 875 868 85 1071 3613
Fragments arranged by size:
3613 1071 875 868 732 107 85
BsmI GAATG_Cn'
Cuts at: 2728 4862 4955 2728
Size: 2134 93 5124
Fragments arranged by size:
5124 2134 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2085 2130 2240 2367 2830 2937
Size: 399 222 73 45 110 127 463 107
Cuts at: 2937 3669 4224 4544 4670 5412 5497 6568 1391
Size: 732 555 320 126 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 732 555 463 399 320
222 127 126 110 107 85 73 45
BspEI T'CCGG_A
Cuts at: 3613 7014 3613
Size: 3401 3950
BspHI T'CATG_A
Cuts at: 4377 5240 6970 4377
Size: 863 1730 4758
Fragments arranged by size:
4758 1730 863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2041 4706 844
Size: 1197 2665 3489
Fragments arranged by size:
3489 2665 1197
BsrBI CCG'CTC
Cuts at: 1394 1823 4208 5238 1394
Size: 429 2385 1030 3507
Fragments arranged by size:
3507 2385 1030 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2676 5846 6020 65
Size: 1183 1428 3170 174 1396
Fragments arranged by size:
3170 1428 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2186 5995 6182 1700
Size: 110 376 3809 187 2869
Fragments arranged by size:
3809 2869 376 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7351
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7351
BssSI C'ACGA_G
Cuts at: 4496 5411 6423 4496
Size: 915 1012 5424
Fragments arranged by size:
5424 1012 915
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7351
BstBI TT'CG_AA
Cuts at: 6155 6155
Size: 7351
BstEII G'GTnAC_C
Cuts at: 1184 2758 2989 1184
Size: 1574 231 5546
Fragments arranged by size:
5546 1574 231
BstXI CCAn_nnnn'nTGG
Cuts at: 3001 3601 3001
Size: 600 6751
BstYI r'GATC_y
Cuts at: 1972 2289 2734 2926 4681 5048 5433 5450 6902
Size: 317 445 192 1755 367 385 17 1452
Cuts at: 6902 6988 7000 7346 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1755 1452 445 385 367 346 317
192 86 17 12
BstZ17I GTA'TAC
Cuts at: 3027 3241 3027
Size: 214 7137
Bsu36I CC'TnA_GG
Cuts at: 3831 3831
Size: 7351
BtgI C'CryG_G
Cuts at: 513 1065 2004 3426 513
Size: 552 939 1422 4438
Fragments arranged by size:
4438 1422 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2887 487
Size: 683 56 1661 4951
Fragments arranged by size:
4951 1661 683 56
BtsI GCAGTG_nn'
Cuts at: 3375 3528 3588 4867 5661 5681 3375
Size: 153 60 1279 794 20 5045
Fragments arranged by size:
5045 1279 794 153 60 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7351
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7316
DraI TTT'AAA
Cuts at: 4655 4655
Size: 7351
DraIII CAC_nnn'GTG
Cuts at: 2707 2707
Size: 7351
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6358 809
Size: 507 5042 1802
Fragments arranged by size:
5042 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2584 4798 5687
Size: 54 1425 50 275 18 754 2214 889
Cuts at: 5687 7316 8
Size: 1629 43
Fragments arranged by size:
2214 1629 1425 889 754 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4798 1537
Size: 275 2986 4090
Fragments arranged by size:
4090 2986 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2456 2699 2835 4284 5281 1353
Size: 1103 243 136 1449 997 3423
Fragments arranged by size:
3423 1449 1103 997 243 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4299 5938 6312 6458 387
Size: 1064 138 2710 1639 374 146 1280
Fragments arranged by size:
2710 1639 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2935 4542 2935
Size: 1607 5744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2666 3623 3705 4365 5414 6011
Size: 393 530 504 957 82 660 1049 597
Cuts at: 6011 6798 7066 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1049 957 787 660 597 530 504
393 268 82
EcoO109I rG'GnC_Cy
Cuts at: 2009 4327 2009
Size: 2318 5033
EcoRI G'AATT_C
Cuts at: 2786 4152 4660 5038 2786
Size: 1366 508 378 5099
Fragments arranged by size:
5099 1366 508 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2846 3991 2846
Size: 1145 6206
FspI TGC'GCA
Cuts at: 5857 5857
Size: 7351
HaeII r_GCGC'y
Cuts at: 1678 2036 2137 6498 1678
Size: 358 101 4361 2531
Fragments arranged by size:
4361 2531 358 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2139 2171 2644 2676 3115
Size: 32 135 32 567 32 473 32 439
Cuts at: 3115 3147 3258 3290 4197 4229 5992 6024 6066
Size: 32 111 32 907 32 1763 32 42
Cuts at: 6066 6098 1373
Size: 32 2626
Fragments arranged by size:
2626 1763 907 567 473 439 135 111
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2192 4695 4941 669
Size: 988 281 254 2503 246 3079
Fragments arranged by size:
3079 2503 988 281 254 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7351
HpaI GTT'AAC
Cuts at: 4941 4941
Size: 7351
KasI G'GCGC_C
Cuts at: 2133 2133
Size: 7351
KpnI G_GTAC'C
Cuts at: 3386 4676 3386
Size: 1290 6061
MfeI C'AATT_G
Cuts at: 4950 4950
Size: 7351
MluI A'CGCG_T
Cuts at: 7227 7227
Size: 7351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2568 2739 6465 6649 1305
Size: 1263 171 3726 184 2007
Fragments arranged by size:
3726 2007 1263 184 171
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2586 10
Size: 54 1425 343 754 4775
Fragments arranged by size:
4775 1425 754 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2984 3461 3551 5309 5668 5827 518
Size: 648 1818 477 90 1758 359 159 2042
Fragments arranged by size:
2042 1818 1758 648 477 359 159 90
MspA1I CmG'CkG
Cuts at: 1606 2490 3086 4079 4318 4767 5444 6592 6837
Size: 884 596 993 239 449 677 1148 245
Cuts at: 6837 1606
Size: 2120
Fragments arranged by size:
2120 1148 993 884 677 596 449 245
239
NarI GG'CG_CC
Cuts at: 2134 2134
Size: 7351
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5860
Fragments arranged by size:
5860 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7351
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7351
NotI GC'GGCC_GC
Cuts at: 4798 4798
Size: 7351
NspI r_CATG'y
Cuts at: 1132 1229 4630 4709 6254 1132
Size: 97 3401 79 1545 2229
Fragments arranged by size:
3401 2229 1545 97 79
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7351
PciI A'CATG_T
Cuts at: 4626 6250 4626
Size: 1624 5727
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2299 1108
Size: 1191 6160
PfoI T'CCnGG_A
Cuts at: 2833 2833
Size: 7351
PmeI GTTT'AAAC
Cuts at: 4655 4655
Size: 7351
PmlI CAC'GTG
Cuts at: 3670 3670
Size: 7351
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5420 5452 6933 6965 5420
Size: 32 1481 32 5806
Fragments arranged by size:
5806 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7351
PsiI TTA'TAA
Cuts at: 4921 4921
Size: 7351
PspOMI G'GGCC_C
Cuts at: 2008 2008
Size: 7351
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3668 3700 3668
Size: 32 7319
PstI C_TGCA'G
Cuts at: 830 2052 4703 830
Size: 1222 2651 3478
Fragments arranged by size:
3478 2651 1222
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7351
PvuII CAG'CTG
Cuts at: 2490 3086 4318 2490
Size: 596 1232 5523
Fragments arranged by size:
5523 1232 596
SacI G_AGCT'C
Cuts at: 2937 4544 2937
Size: 1607 5744
SalI G'TCGA_C
Cuts at: 1936 2190 4693 1936
Size: 254 2503 4594
Fragments arranged by size:
4594 2503 254
SapI GCTCTTCn'nnn_
Cuts at: 2835 2835
Size: 7351
SbfI CC_TGCA'GG
Cuts at: 4703 4703
Size: 7351
ScaI AGT'ACT
Cuts at: 5599 7311 5599
Size: 1712 5639
SexAI A'CCwGG_T
Cuts at: 2526 2526
Size: 7351
SfcI C'TryA_G
Cuts at: 826 1045 2048 2323 2572 3705 4087 4699 5834
Size: 219 1003 275 249 1133 382 612 1135
Cuts at: 5834 6515 6706 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1133 1003 681 612 382 275
249 219 191
SfoI GGC'GCC
Cuts at: 2135 2135
Size: 7351
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7351
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7351
SmaI CCC'GGG
Cuts at: 1872 4250 4678 1872
Size: 2378 428 4545
Fragments arranged by size:
4545 2378 428
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4004 4309 5455 6356 6618 820
Size: 197 926 2061 305 1146 901 262 1553
Fragments arranged by size:
2061 1553 1146 926 901 305 262 197
SnaBI TAC'GTA
Cuts at: 493 3024 5142 493
Size: 2531 2118 2702
Fragments arranged by size:
2702 2531 2118
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7351
SphI G_CATG'C
Cuts at: 1132 4709 1132
Size: 3577 3774
SspI AAT'ATT
Cuts at: 5 52 5275 5
Size: 47 5223 2081
Fragments arranged by size:
5223 2081 47
StuI AGG'CCT
Cuts at: 1517 2266 2393 1517
Size: 749 127 6475
Fragments arranged by size:
6475 749 127
StyI C'CwwG_G
Cuts at: 513 1065 2004 2012 2587 4790 513
Size: 552 939 8 575 2203 3074
Fragments arranged by size:
3074 2203 939 575 552 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2807 3021 3974 5378 5395 5548 5733
Size: 1255 1227 214 953 1404 17 153 185
Cuts at: 5733 6178 325
Size: 445 1498
Fragments arranged by size:
1498 1404 1255 1227 953 445 214 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3351 3927 5597
Size: 274 80 33 51 1443 1374 576 1670
Cuts at: 5597 7309 96
Size: 1712 138
Fragments arranged by size:
1712 1670 1443 1374 576 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2829 2861 3271 3303 7311 7343 2829
Size: 32 410 32 4008 32 2837
Fragments arranged by size:
4008 2837 410 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7351
XbaI T'CTAG_A
Cuts at: 4687 4687
Size: 7351
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3424 3424
Size: 7351
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7351
XmaI C'CCGG_G
Cuts at: 1870 4248 4676 1870
Size: 2378 428 4545
Fragments arranged by size:
4545 2378 428
XmnI GAAnn'nnTTC
Cuts at: 2332 4519 5480 2332
Size: 2187 961 4203
Fragments arranged by size:
4203 2187 961
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5159 276
Size: 53 83 186 936 3625 2468
Fragments arranged by size:
3625 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI EcoNI EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PpuMI PspXI RsrII SacII SanDI
SfiI SrfI SwaI