Restriction map for
pFN21AE2111
(Circular) (Six-base) MAPSORT of: pFN21AE2111.seq Check: 2707 from: 1 to: 7183
pFN21AE2111 7183 bp
With 182 enzymes: SgfI *
March 28, 2012 12:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3640 3640
Size: 7183
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4144 4993 278
Size: 53 83 186 936 2608 849 2468
Fragments arranged by size:
2608 2468 936 849 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2854 4526 1937
Size: 917 1672 4594
Fragments arranged by size:
4594 1672 917
Acc65I G'GTAC_C
Cuts at: 4504 4504
Size: 7183
AclI AA'CG_TT
Cuts at: 1563 5310 5683 1563
Size: 3747 373 3063
Fragments arranged by size:
3747 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2342 2359 2438 3263 5246 6630 6898 1632
Size: 710 17 79 825 1983 1384 268 1917
Fragments arranged by size:
1983 1917 1384 825 710 268 79 17
AflII C'TTAA_G
Cuts at: 820 1017 2609 820
Size: 197 1592 5394
Fragments arranged by size:
5394 1592 197
AflIII A'CryG_T
Cuts at: 1203 3604 6082 7059 1203
Size: 2401 2478 977 1327
Fragments arranged by size:
2478 2401 1327 977
AgeI A'CCGG_T
Cuts at: 6014 6014
Size: 7183
AhdI GACnn_n'nnGTC
Cuts at: 3001 5912 3001
Size: 2911 4272
AleI CACnn'nnGTG
Cuts at: 1166 3857 4292 1166
Size: 2691 435 4057
Fragments arranged by size:
4057 2691 435
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3086 3875 1184
Size: 1902 789 4492
Fragments arranged by size:
4492 1902 789
AlwNI CAG_nnn'CTG
Cuts at: 2081 2136 2815 2980 4926 6498 2081
Size: 55 679 165 1946 1572 2766
Fragments arranged by size:
2766 1946 1572 679 165 55
ApaI G_GGCC'C
Cuts at: 3297 3679 3297
Size: 382 6801
ApaLI G'TGCA_C
Cuts at: 5240 6396 5240
Size: 1156 6027
ApoI r'AATT_y
Cuts at: 1493 4492 4718 4870 6979 1493
Size: 2999 226 152 2109 1697
Fragments arranged by size:
2999 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 4133 4133
Size: 7183
AseI AT'TA_AT
Cuts at: 160 1032 5737 160
Size: 872 4705 1606
Fragments arranged by size:
4705 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7183
AvaI C'yCGr_G
Cuts at: 1870 1943 2113 2836 3535 3729 4508 1870
Size: 73 170 723 699 194 779 4545
Fragments arranged by size:
4545 779 723 699 194 170 73
BamHI G'GATC_C
Cuts at: 2888 3030 3045 4513 4880 2888
Size: 142 15 1468 367 5191
Fragments arranged by size:
5191 1468 367 142 15
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3100 3133 3336 3527 3859
Size: 325 217 627 1313 33 203 191 332
Cuts at: 3859 3866 3939 4504 5959 618
Size: 7 73 565 1455 1842
Fragments arranged by size:
1842 1455 1313 627 565 332 325 217
203 191 73 33 7
BanII G_rGCy'C
Cuts at: 1391 2391 2657 2681 3180 3297 3381 3679 4502
Size: 1000 266 24 499 117 84 298 823
Cuts at: 4502 1391
Size: 4072
Fragments arranged by size:
4072 1000 823 499 298 266 117 84
24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2806 3954 928
Size: 971 907 1148 4157
Fragments arranged by size:
4157 1148 971 907
BbvCI CC'TCA_GC
Cuts at: 3440 4035 3440
Size: 595 6588
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5374 5408 1903
Size: 34 3437 34 3678
Fragments arranged by size:
3678 3437 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2705 5075 6285 2705
Size: 2370 1210 3603
Fragments arranged by size:
3603 2370 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7183
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3301 3827 5794
Size: 107 122 71 631 605 1629 526 1967
Cuts at: 5794 136
Size: 1525
Fragments arranged by size:
1967 1629 1525 631 605 526 122 107
71
BglII A'GATC_T
Cuts at: 7178 7178
Size: 7183
BlpI GC'TnA_GC
Cuts at: 4600 4600
Size: 7183
Bme1580I G_kGCm'C
Cuts at: 1790 3105 3297 3498 3543 3679 4228 5244 6400
Size: 1315 192 201 45 136 549 1016 1156
Cuts at: 6400 1790
Size: 2573
Fragments arranged by size:
2573 1315 1156 1016 549 201 192 136
45
BmgBI CAC'GTC
Cuts at: 1318 3607 3619 1318
Size: 2289 12 4882
Fragments arranged by size:
4882 2289 12
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2069 3530 4914
Size: 416 53 83 399 24 652 1461 1384
Cuts at: 4914 5872 442
Size: 958 1753
Fragments arranged by size:
1753 1461 1384 958 652 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2405 2833 2843 3212 3251 3982 4145 4166
Size: 1166 428 10 369 39 731 163 21
Cuts at: 4166 5843 1239
Size: 1677 2579
Fragments arranged by size:
2579 1677 1166 731 428 369 163 39
21 10
Bpu10I CC'TnA_GC
Cuts at: 3440 4035 3440
Size: 595 6588
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4034 5308 6173 6471 4034
Size: 1274 865 298 4746
Fragments arranged by size:
4746 1274 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2848 5846 882
Size: 617 1349 2998 2219
Fragments arranged by size:
2998 2219 1349 617
BsaAI yAC'GTr
Cuts at: 493 1204 3126 3427 3499 3553 4974 493
Size: 711 1922 301 72 54 1421 2702
Fragments arranged by size:
2702 1922 1421 711 301 72 54
BsaBI GATnn'nnATC
Cuts at: 7177 7177
Size: 7183
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4141 4990 5372 6007
Size: 53 83 186 936 2608 849 382 635
Cuts at: 6007 275
Size: 1451
Fragments arranged by size:
2608 1451 936 849 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4041 5616 6014 6288 6435 6846 4041
Size: 1575 398 274 147 411 4378
Fragments arranged by size:
4378 1575 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2085 2115 2167 2197 1178
Size: 30 165 30 682 30 52 30 6164
Fragments arranged by size:
6164 682 165 52 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 2420 2836 3188 3236 3248 3275 3410
Size: 916 318 416 352 48 12 27 135
Cuts at: 3410 4142 4163 4424 4463 1186
Size: 732 21 261 39 3906
Fragments arranged by size:
3906 916 732 416 352 318 261 135
48 39 27 21 12
BseYI C'CCAG_C
Cuts at: 2944 4099 4119 4227 6386 6882 2944
Size: 1155 20 108 2159 496 3245
Fragments arranged by size:
3245 2159 1155 496 108 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2350 3104 3221 3575 4073 1163
Size: 573 614 754 117 354 498 4273
Fragments arranged by size:
4273 754 614 573 498 354 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3585 4633 5394 6422 1540
Size: 275 186 1584 1048 761 1028 2301
Fragments arranged by size:
2301 1584 1048 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2681 3180 3426 4020 4177 5244 5329 6400 2681
Size: 499 246 594 157 1067 85 1071 3464
Fragments arranged by size:
3464 1071 1067 594 499 246 157 85
BsmI GAATG_Cn'
Cuts at: 2272 4694 4787 2272
Size: 2422 93 4668
Fragments arranged by size:
4668 2422 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2775 2775
Size: 7183
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2391 2657 2681 3105 3180 3297 3381
Size: 399 601 266 24 424 75 117 84
Cuts at: 3381 3426 3498 3543 3679 4020 4177 4228 4502
Size: 45 72 45 136 341 157 51 274
Cuts at: 4502 5244 5329 6400 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 601 424 399 341 274
266 157 136 117 85 84 75 72
51 45 45 24
BspEI T'CCGG_A
Cuts at: 4041 6846 4041
Size: 2805 4378
BspHI T'CATG_A
Cuts at: 2520 3988 5072 6802 2520
Size: 1468 1084 1730 2901
Fragments arranged by size:
2901 1730 1468 1084
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2724 3076 3640 4538 844
Size: 1880 352 564 898 3489
Fragments arranged by size:
3489 1880 898 564 352
BsrBI CCG'CTC
Cuts at: 1394 1823 3238 5070 1394
Size: 429 1415 1832 3507
Fragments arranged by size:
3507 1832 1415 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5678 5852 65
Size: 1183 4430 174 1396
Fragments arranged by size:
4430 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2927 3584 3950 4028 5827 6014 1700
Size: 110 1117 657 366 78 1799 187 2869
Fragments arranged by size:
2869 1799 1117 657 366 187 110 78
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7183
BssHII G'CGCG_C
Cuts at: 1925 4133 1925
Size: 2208 4975
BssSI C'ACGA_G
Cuts at: 3392 5243 6255 3392
Size: 1851 1012 4320
Fragments arranged by size:
4320 1851 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2278 794
Size: 1484 5699
BstBI TT'CG_AA
Cuts at: 5987 5987
Size: 7183
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7183
BstXI CCAn_nnnn'nTGG
Cuts at: 2681 3464 2681
Size: 783 6400
BstYI r'GATC_y
Cuts at: 1972 2197 2888 3030 3045 4513 4880 5265 5282
Size: 225 691 142 15 1468 367 385 17
Cuts at: 5282 6734 6820 6832 7178 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1468 1452 691 385 367 346 225
142 86 17 15 12
Bsu36I CC'TnA_GG
Cuts at: 2220 2220
Size: 7183
BtgI C'CryG_G
Cuts at: 513 1065 3715 3955 513
Size: 552 2650 240 3741
Fragments arranged by size:
3741 2650 552 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3003 3461 487
Size: 683 56 1777 458 4209
Fragments arranged by size:
4209 1777 683 458 56
BtsI GCAGTG_nn'
Cuts at: 2233 2267 3911 4699 5493 5513 2233
Size: 34 1644 788 794 20 3903
Fragments arranged by size:
3903 1644 794 788 34 20
ClaI AT'CG_AT
Cuts at: 1554 2535 1554
Size: 981 6202
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2232 2267 529
Size: 35 1668 35 5445
Fragments arranged by size:
5445 1668 35 35
DraI TTT'AAA
Cuts at: 4487 4487
Size: 7183
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4430 6190 809
Size: 507 3114 1760 1802
Fragments arranged by size:
3114 1802 1760 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 3451 3472
Size: 54 1425 50 275 18 348 1273 21
Cuts at: 3472 3501 3582 3952 4630 5519 7148 8
Size: 29 81 370 678 889 1629 43
Fragments arranged by size:
1629 1425 1273 889 678 370 348 275
81 54 50 43 29 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3582 4630 1537
Size: 275 1770 1048 4090
Fragments arranged by size:
4090 1770 1048 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2405 3836 4073 5113 1353
Size: 1052 1431 237 1040 3423
Fragments arranged by size:
3423 1431 1052 1040 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5770 6144 6290 387
Size: 1064 138 4181 374 146 1280
Fragments arranged by size:
4181 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2679 3178 2679
Size: 499 6684
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 2359 2405 2438 2833 2843 3212
Size: 393 710 17 46 33 395 10 369
Cuts at: 3212 3251 3263 3982 4145 4166 5246 5843 6630
Size: 39 12 719 163 21 1080 597 787
Cuts at: 6630 6898 1239
Size: 268 1524
Fragments arranged by size:
1524 1080 787 719 710 597 395 393
369 268 163 46 39 33 21 17
12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2324 2324
Size: 7183
EcoO109I rG'GnC_Cy
Cuts at: 3293 3294 3675 3697 3726 3293
Size: 1 381 22 29 6750
Fragments arranged by size:
6750 381 29 22 1
EcoRI G'AATT_C
Cuts at: 4492 4870 4492
Size: 378 6805
FseI GG_CCGG'CC
Cuts at: 3588 4032 3588
Size: 444 6739
FspI TGC'GCA
Cuts at: 3254 5689 3254
Size: 2435 4748
FspAI rTGC'GCAy
Cuts at: 3254 3254
Size: 7183
HaeII r_GCGC'y
Cuts at: 1678 2194 3756 3849 6330 1678
Size: 516 1562 93 2481 2531
Fragments arranged by size:
2531 2481 1562 516 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2167 2199 2663 2695 2987
Size: 32 135 32 595 32 464 32 292
Cuts at: 2987 3019 3272 3304 5824 5856 5898 5930 1373
Size: 32 253 32 2520 32 42 32 2626
Fragments arranged by size:
2626 2520 595 464 292 253 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2908 4527 4773 669
Size: 988 281 970 1619 246 3079
Fragments arranged by size:
3079 1619 988 970 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7183
HpaI GTT'AAC
Cuts at: 4773 4773
Size: 7183
KpnI G_GTAC'C
Cuts at: 4508 4508
Size: 7183
MfeI C'AATT_G
Cuts at: 4782 4782
Size: 7183
MluI A'CGCG_T
Cuts at: 7059 7059
Size: 7183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6297 6481 1305
Size: 4992 184 2007
Fragments arranged by size:
4992 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3474 3503 10
Size: 54 1425 343 1642 29 3690
Fragments arranged by size:
3690 1642 1425 343 54 29
MslI CAynn'nnrTG
Cuts at: 518 1166 3714 3857 4247 4292 5141 5500 5659
Size: 648 2548 143 390 45 849 359 159
Cuts at: 5659 518
Size: 2042
Fragments arranged by size:
2548 2042 849 648 390 359 159 143
45
MspA1I CmG'CkG
Cuts at: 1606 2530 2831 3354 4123 4599 5276 6424 6669
Size: 924 301 523 769 476 677 1148 245
Cuts at: 6669 1606
Size: 2120
Fragments arranged by size:
2120 1148 924 769 677 523 476 301
245
NaeI GCC'GGC
Cuts at: 2929 3586 4030 2929
Size: 657 444 6082
Fragments arranged by size:
6082 657 444
NcoI C'CATG_G
Cuts at: 513 1065 3715 513
Size: 552 2650 3981
Fragments arranged by size:
3981 2650 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7183
NgoMIV G'CCGG_C
Cuts at: 2927 3584 4028 2927
Size: 657 444 6082
Fragments arranged by size:
6082 657 444
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7183
NotI GC'GGCC_GC
Cuts at: 4630 4630
Size: 7183
NspI r_CATG'y
Cuts at: 1132 1229 4541 6086 1132
Size: 97 3312 1545 2229
Fragments arranged by size:
3312 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3299 4023 4315 1348
Size: 1951 724 292 4216
Fragments arranged by size:
4216 1951 724 292
PciI A'CATG_T
Cuts at: 6082 6082
Size: 7183
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2081 2761 3190 1108
Size: 973 680 429 5101
Fragments arranged by size:
5101 973 680 429
PfoI T'CCnGG_A
Cuts at: 2141 2820 3381 3414 2141
Size: 679 561 33 5910
Fragments arranged by size:
5910 679 561 33
PmeI GTTT'AAAC
Cuts at: 4487 4487
Size: 7183
PmlI CAC'GTG
Cuts at: 3499 3499
Size: 7183
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5252 5284 6765 6797 5252
Size: 32 1481 32 5638
Fragments arranged by size:
5638 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3726 3726
Size: 7183
PshAI GACnn'nnGTC
Cuts at: 1613 4006 1613
Size: 2393 4790
PsiI TTA'TAA
Cuts at: 4753 4753
Size: 7183
PspOMI G'GGCC_C
Cuts at: 3293 3675 3293
Size: 382 6801
PstI C_TGCA'G
Cuts at: 830 4535 830
Size: 3705 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7183
PvuII CAG'CTG
Cuts at: 2530 2831 4123 2530
Size: 301 1292 5590
Fragments arranged by size:
5590 1292 301
SacI G_AGCT'C
Cuts at: 2681 3180 2681
Size: 499 6684
SalI G'TCGA_C
Cuts at: 1936 4525 1936
Size: 2589 4594
SapI GCTCTTCn'nnn_
Cuts at: 3836 3836
Size: 7183
SbfI CC_TGCA'GG
Cuts at: 4535 4535
Size: 7183
ScaI AGT'ACT
Cuts at: 5431 7143 5431
Size: 1712 5471
SfcI C'TryA_G
Cuts at: 826 1045 2128 2665 4354 4531 5666 6347 6538
Size: 219 1083 537 1689 177 1135 681 191
Cuts at: 6538 826
Size: 1471
Fragments arranged by size:
1689 1471 1135 1083 681 537 219 191
177
SfiI GGCCn_nnn'nGGCC
Cuts at: 3301 3301
Size: 7183
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7183
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7183
SmaI CCC'GGG
Cuts at: 1872 4510 1872
Size: 2638 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2609 4013 5287 6188 6450 820
Size: 197 926 666 1404 1274 901 262 1553
Fragments arranged by size:
1553 1404 1274 926 901 666 262 197
SnaBI TAC'GTA
Cuts at: 493 4974 493
Size: 4481 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7183
SphI G_CATG'C
Cuts at: 1132 4541 1132
Size: 3409 3774
SspI AAT'ATT
Cuts at: 5 52 5107 5
Size: 47 5055 2081
Fragments arranged by size:
5055 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7183
StyI C'CwwG_G
Cuts at: 513 1065 2031 3642 3715 4622 513
Size: 552 966 1611 73 907 3074
Fragments arranged by size:
3074 1611 966 907 552 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2579 4113 5210 5227 5380 5565 6010
Size: 1255 999 1534 1097 17 153 185 445
Cuts at: 6010 325
Size: 1498
Fragments arranged by size:
1534 1498 1255 1097 999 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2445 5429 7141
Size: 274 80 33 51 1443 468 2984 1712
Cuts at: 7141 96
Size: 138
Fragments arranged by size:
2984 1712 1443 468 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2085 2117 2732 2764 7143 7175 2085
Size: 32 615 32 4379 32 2093
Fragments arranged by size:
4379 2093 615 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7183
XbaI T'CTAG_A
Cuts at: 4519 4519
Size: 7183
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2253 3511 3649 2253
Size: 1258 138 5787
Fragments arranged by size:
5787 1258 138
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7183
XmaI C'CCGG_G
Cuts at: 1870 4508 1870
Size: 2638 4545
XmnI GAAnn'nnTTC
Cuts at: 5312 5312
Size: 7183
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4142 4991 276
Size: 53 83 186 936 2608 849 2468
Fragments arranged by size:
2608 2468 936 849 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FalI KasI NarI NruI NsiI
PacI PspXI PsrI RsrII SacII SanDI SexAI SfoI
SrfI SwaI