Restriction map for
pFN21AE2109
(Circular) (Six-base) MAPSORT of: pFN21AE2109.seq Check: 64 from: 1 to: 7495
pFN21AE2109 7495 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5305 278
Size: 53 83 186 936 3769 2468
Fragments arranged by size:
3769 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3045 3327 4782 4838 1937
Size: 1108 282 1455 56 4594
Fragments arranged by size:
4594 1455 1108 282 56
Acc65I G'GTAC_C
Cuts at: 4816 4816
Size: 7495
AclI AA'CG_TT
Cuts at: 1563 5622 5995 1563
Size: 4059 373 3063
Fragments arranged by size:
4059 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2574 3811 4104 4439 4740 5558 6942 7210
Size: 942 1237 293 335 301 818 1384 268
Cuts at: 7210 1632
Size: 1917
Fragments arranged by size:
1917 1384 1237 942 818 335 301 293
268
AfeI AGC'GCT
Cuts at: 2910 2910
Size: 7495
AflII C'TTAA_G
Cuts at: 820 1017 2254 820
Size: 197 1237 6061
Fragments arranged by size:
6061 1237 197
AflIII A'CryG_T
Cuts at: 1203 6394 7371 1203
Size: 5191 977 1327
Fragments arranged by size:
5191 1327 977
AgeI A'CCGG_T
Cuts at: 6326 6326
Size: 7495
AhdI GACnn_n'nnGTC
Cuts at: 2640 2652 6224 2640
Size: 12 3572 3911
Fragments arranged by size:
3911 3572 12
AleI CACnn'nnGTG
Cuts at: 1166 4280 1166
Size: 3114 4381
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2261 4580 1184
Size: 1077 2319 4099
Fragments arranged by size:
4099 2319 1077
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4369 4401 4369
Size: 32 7463
AlwNI CAG_nnn'CTG
Cuts at: 2485 3442 4048 4178 4295 4554 5238 6810 2485
Size: 957 606 130 117 259 684 1572 3170
Fragments arranged by size:
3170 1572 957 684 606 259 130 117
ApaI G_GGCC'C
Cuts at: 2704 3172 3263 3429 2704
Size: 468 91 166 6770
Fragments arranged by size:
6770 468 166 91
ApaLI G'TGCA_C
Cuts at: 5552 6708 5552
Size: 1156 6339
ApoI r'AATT_y
Cuts at: 1493 3894 4804 5030 5182 7291 1493
Size: 2401 910 226 152 2109 1697
Fragments arranged by size:
2401 2109 1697 910 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6049 160
Size: 872 5017 1606
Fragments arranged by size:
5017 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7495
AvaI C'yCGr_G
Cuts at: 1870 1943 2771 3313 3422 4112 4648 4684 4820
Size: 73 828 542 109 690 536 36 136
Cuts at: 4820 1870
Size: 4545
Fragments arranged by size:
4545 828 690 542 536 136 109 73
36
AvrII C'CTAG_G
Cuts at: 2063 2063
Size: 7495
BamHI G'GATC_C
Cuts at: 4825 5192 4825
Size: 367 7128
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3097 4564 4816 6271 618
Size: 325 217 627 1310 1467 252 1455 1842
Fragments arranged by size:
1842 1467 1455 1310 627 325 252 217
BanII G_rGCy'C
Cuts at: 1391 2104 2398 2704 3172 3263 3423 3429 3555
Size: 713 294 306 468 91 160 6 126
Cuts at: 3555 4008 4244 4814 1391
Size: 453 236 570 4072
Fragments arranged by size:
4072 713 570 468 453 306 294 236
160 126 91 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6524
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5686 5720 1903
Size: 34 3749 34 3678
Fragments arranged by size:
3749 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4475 5387 6597 4475
Size: 912 1210 5373
Fragments arranged by size:
5373 1210 912
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7495
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3114 3258 3780
Size: 107 122 71 631 605 1442 144 522
Cuts at: 3780 6106 136
Size: 2326 1525
Fragments arranged by size:
2326 1525 1442 631 605 522 144 122
107 71
BglII A'GATC_T
Cuts at: 7490 7490
Size: 7495
BlpI GC'TnA_GC
Cuts at: 4912 4912
Size: 7495
Bme1580I G_kGCm'C
Cuts at: 1790 2704 3102 3172 3263 3429 5556 6712 1790
Size: 914 398 70 91 166 2127 1156 2573
Fragments arranged by size:
2573 2127 1156 914 398 166 91 70
BmgBI CAC'GTC
Cuts at: 1318 2970 2979 1318
Size: 1652 9 5834
Fragments arranged by size:
5834 1652 9
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2495 4255 5226
Size: 416 53 83 399 24 1078 1760 971
Cuts at: 5226 6184 442
Size: 958 1753
Fragments arranged by size:
1760 1753 1078 971 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2334 2487 3153 3194 3218 3323 3820 4129
Size: 1095 153 666 41 24 105 497 309
Cuts at: 4129 4203 6155 1239
Size: 74 1952 2579
Fragments arranged by size:
2579 1952 1095 666 497 309 153 105
74 41 24
Bpu10I CC'TnA_GC
Cuts at: 2759 2759
Size: 7495
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2168 2650 2659 3740 5620 6485 6783 2168
Size: 482 9 1081 1880 865 298 2880
Fragments arranged by size:
2880 1880 1081 865 482 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2521 6158 882
Size: 617 1022 3637 2219
Fragments arranged by size:
3637 2219 1022 617
BsaAI yAC'GTr
Cuts at: 493 1204 5286 493
Size: 711 4082 2702
Fragments arranged by size:
4082 2702 711
BsaBI GATnn'nnATC
Cuts at: 7489 7489
Size: 7495
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2627 5302 5684 6319
Size: 53 83 186 936 1094 2675 382 635
Cuts at: 6319 275
Size: 1451
Fragments arranged by size:
2675 1451 1094 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2037 5928 6326 6600 6747 7158 2037
Size: 3891 398 274 147 411 2374
Fragments arranged by size:
3891 2374 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4238 4241 4268 4271 4369
Size: 30 165 30 2835 3 27 3 98
Cuts at: 4369 4399 1178
Size: 30 4274
Fragments arranged by size:
4274 2835 165 98 30 30 30 27
3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2202 2214 3320 3345 3563 4246 4314 4765
Size: 1016 12 1106 25 218 683 68 451
Cuts at: 4765 1186
Size: 3916
Fragments arranged by size:
3916 1106 1016 683 451 218 68 25
12
BseYI C'CCAG_C
Cuts at: 3084 3435 4178 4395 6698 7194 3084
Size: 351 743 217 2303 496 3385
Fragments arranged by size:
3385 2303 743 496 351 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3263 4061 1163
Size: 573 1527 798 4597
Fragments arranged by size:
4597 1527 798 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3279 4945 5706 6734 1540
Size: 275 186 1278 1666 761 1028 2301
Fragments arranged by size:
2301 1666 1278 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3180 3204 3423 3555 4008 5556 5641 6712 3180
Size: 24 219 132 453 1548 85 1071 3963
Fragments arranged by size:
3963 1548 1071 453 219 132 85 24
BsmI GAATG_Cn'
Cuts at: 2569 3078 5006 5099 2569
Size: 509 1928 93 4965
Fragments arranged by size:
4965 1928 509 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2104 2398 2704 3102 3172 3180 3204
Size: 399 314 294 306 398 70 8 24
Cuts at: 3204 3263 3423 3429 3555 4008 4244 4814 5556
Size: 59 160 6 126 453 236 570 742
Cuts at: 5556 5641 6712 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 570 453 399 398 314
306 294 236 160 126 85 70 59
24 8 6
BspEI T'CCGG_A
Cuts at: 7158 7158
Size: 7495
BspHI T'CATG_A
Cuts at: 5384 7114 5384
Size: 1730 5765
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4026 4850 844
Size: 3182 824 3489
Fragments arranged by size:
3489 3182 824
BsrBI CCG'CTC
Cuts at: 1394 1823 2895 3349 5382 1394
Size: 429 1072 454 2033 3507
Fragments arranged by size:
3507 2033 1072 454 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2565 3502 5990 6164 65
Size: 1183 1317 937 2488 174 1396
Fragments arranged by size:
2488 1396 1317 1183 937 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3016 3148 3274 3278 3620 4054 6139
Size: 110 1206 132 126 4 342 434 2085
Cuts at: 6139 6326 1700
Size: 187 2869
Fragments arranged by size:
2869 2085 1206 434 342 187 132 126
110 4
BsrGI T'GTAC_A
Cuts at: 96 4231 96
Size: 4135 3360
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7495
BssSI C'ACGA_G
Cuts at: 3179 3554 5555 6567 3179
Size: 375 2001 1012 4107
Fragments arranged by size:
4107 2001 1012 375
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2165 2564 3253 3504 4048 4313 794
Size: 1371 399 689 251 544 265 3976
Fragments arranged by size:
3976 1371 689 544 399 265 251
BstBI TT'CG_AA
Cuts at: 6299 6299
Size: 7495
BstEII G'GTnAC_C
Cuts at: 1184 2302 2455 1184
Size: 1118 153 6224
Fragments arranged by size:
6224 1118 153
BstXI CCAn_nnnn'nTGG
Cuts at: 2983 4475 2983
Size: 1492 6003
BstYI r'GATC_y
Cuts at: 1972 2752 3759 4825 5192 5577 5594 7046 7132
Size: 780 1007 1066 367 385 17 1452 86
Cuts at: 7132 7144 7490 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1066 1007 780 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2194 2194
Size: 7495
BtgI C'CryG_G
Cuts at: 513 1065 2722 4092 4474 4599 4749 513
Size: 552 1657 1370 382 125 150 3259
Fragments arranged by size:
3259 1657 1370 552 382 150 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6756
Fragments arranged by size:
6756 683 56
BtsI GCAGTG_nn'
Cuts at: 4564 5011 5805 5825 4564
Size: 447 794 20 6234
Fragments arranged by size:
6234 794 447 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7495
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7460
DraI TTT'AAA
Cuts at: 2340 4799 2340
Size: 2459 5036
DraIII CAC_nnn'GTG
Cuts at: 3238 3238
Size: 7495
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2782 4586 6502 809
Size: 507 1466 1804 1916 1802
Fragments arranged by size:
1916 1804 1802 1466 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3018 3276 4056
Size: 54 1425 50 275 18 1188 258 780
Cuts at: 4056 4942 5831 7460 8
Size: 886 889 1629 43
Fragments arranged by size:
1629 1425 1188 889 886 780 275 258
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3276 4942 1537
Size: 275 1464 1666 4090
Fragments arranged by size:
4090 1666 1464 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2897 4450 5425 1353
Size: 1544 1553 975 3423
Fragments arranged by size:
3423 1553 1544 975
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6082 6456 6602 387
Size: 1064 138 4493 374 146 1280
Fragments arranged by size:
4493 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3421 3553 4006 3421
Size: 132 453 6910
Fragments arranged by size:
6910 453 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2334 2487 2574 3153 3194 3218 3323
Size: 393 702 153 87 579 41 24 105
Cuts at: 3323 3811 3820 4104 4129 4203 4439 4740 5558
Size: 488 9 284 25 74 236 301 818
Cuts at: 5558 6155 6942 7210 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 818 787 702 597 579 488 393
301 284 268 236 153 105 87 74
41 25 24 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2388 3739 4218 2388
Size: 1351 479 5665
Fragments arranged by size:
5665 1351 479
EcoO109I rG'GnC_Cy
Cuts at: 2060 2601 2700 2701 2880 3168 3169 3260 3374
Size: 541 99 1 179 288 1 91 114
Cuts at: 3374 3426 2060
Size: 52 6129
Fragments arranged by size:
6129 541 288 179 114 99 91 52
1 1
EcoRI G'AATT_C
Cuts at: 4804 5182 4804
Size: 378 7117
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2414 3781 2414
Size: 1367 6128
FseI GG_CCGG'CC
Cuts at: 3282 3282
Size: 7495
FspI TGC'GCA
Cuts at: 3402 6001 3402
Size: 2599 4896
FspAI rTGC'GCAy
Cuts at: 3402 3402
Size: 7495
HaeII r_GCGC'y
Cuts at: 1678 2912 3146 3531 6642 1678
Size: 1234 234 385 3111 2531
Fragments arranged by size:
3111 2531 1234 385 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2197 2229 2259 2286 2291
Size: 32 135 32 625 32 30 27 5
Cuts at: 2291 2318 2475 2507 2585 2617 2626 2638 2658
Size: 27 157 32 78 32 9 12 20
Cuts at: 2658 2670 6136 6168 6210 6242 1373
Size: 12 3466 32 42 32 2626
Fragments arranged by size:
3466 2626 625 157 135 78 42 32
32 32 32 32 32 32 30 27
27 20 12 12 9 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3229 4839 5085 669
Size: 988 281 1291 1610 246 3079
Fragments arranged by size:
3079 1610 1291 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7495
HpaI GTT'AAC
Cuts at: 5085 5085
Size: 7495
KpnI G_GTAC'C
Cuts at: 4820 4820
Size: 7495
MfeI C'AATT_G
Cuts at: 5094 5094
Size: 7495
MluI A'CGCG_T
Cuts at: 7371 7371
Size: 7495
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2742 6609 6793 1305
Size: 1437 3867 184 2007
Fragments arranged by size:
3867 2007 1437 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5673
Fragments arranged by size:
5673 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3239 3396 4280 4473 4658 5453 5812
Size: 648 2073 157 884 193 185 795 359
Cuts at: 5812 5971 518
Size: 159 2042
Fragments arranged by size:
2073 2042 884 795 648 359 193 185
159 157
MspA1I CmG'CkG
Cuts at: 1606 2439 2926 3075 3196 3442 3513 3859 4175
Size: 833 487 149 121 246 71 346 316
Cuts at: 4175 4280 4492 4713 4758 4911 5588 6736 6981
Size: 105 212 221 45 153 677 1148 245
Cuts at: 6981 1606
Size: 2120
Fragments arranged by size:
2120 1148 833 677 487 346 316 246
245 221 212 153 149 121 105 71
45
NaeI GCC'GGC
Cuts at: 3018 3150 3276 3280 4056 3018
Size: 132 126 4 776 6457
Fragments arranged by size:
6457 776 132 126 4
NcoI C'CATG_G
Cuts at: 513 1065 4474 4599 513
Size: 552 3409 125 3409
Fragments arranged by size:
3409 3409 552 125
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7495
NgoMIV G'CCGG_C
Cuts at: 3016 3148 3274 3278 4054 3016
Size: 132 126 4 776 6457
Fragments arranged by size:
6457 776 132 126 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7495
NotI GC'GGCC_GC
Cuts at: 4942 4942
Size: 7495
NspI r_CATG'y
Cuts at: 1132 1229 2323 2529 3190 3947 4853 6398 1132
Size: 97 1094 206 661 757 906 1545 2229
Fragments arranged by size:
2229 1545 1094 906 757 661 206 97
PasI CC'CwG_GG
Cuts at: 1348 2689 1348
Size: 1341 6154
PciI A'CATG_T
Cuts at: 6394 6394
Size: 7495
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2384 3238 3442 1108
Size: 1276 854 204 5161
Fragments arranged by size:
5161 1276 854 204
PfoI T'CCnGG_A
Cuts at: 2108 3898 4219 2108
Size: 1790 321 5384
Fragments arranged by size:
5384 1790 321
PmeI GTTT'AAAC
Cuts at: 4799 4799
Size: 7495
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4369 4401 5564 5596 7077 7109 4369
Size: 32 1163 32 1481 32 4755
Fragments arranged by size:
4755 1481 1163 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2601 2880 2601
Size: 279 7216
PshAI GACnn'nnGTC
Cuts at: 1613 4758 1613
Size: 3145 4350
PsiI TTA'TAA
Cuts at: 5065 5065
Size: 7495
PspOMI G'GGCC_C
Cuts at: 2700 3168 3259 3425 2700
Size: 468 91 166 6770
Fragments arranged by size:
6770 468 166 91
PspXI vC'TCGA_Gb
Cuts at: 4684 4684
Size: 7495
PstI C_TGCA'G
Cuts at: 830 3216 3527 4053 4497 4559 4847 830
Size: 2386 311 526 444 62 288 3478
Fragments arranged by size:
3478 2386 526 444 311 288 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7495
PvuII CAG'CTG
Cuts at: 2439 3075 3196 3442 4175 4280 4492 2439
Size: 636 121 246 733 105 212 5442
Fragments arranged by size:
5442 733 636 246 212 121 105
SacI G_AGCT'C
Cuts at: 3423 3555 4008 3423
Size: 132 453 6910
Fragments arranged by size:
6910 453 132
SalI G'TCGA_C
Cuts at: 1936 4837 1936
Size: 2901 4594
SbfI CC_TGCA'GG
Cuts at: 4847 4847
Size: 7495
ScaI AGT'ACT
Cuts at: 5743 7455 5743
Size: 1712 5783
SexAI A'CCwGG_T
Cuts at: 2597 2876 2597
Size: 279 7216
SfcI C'TryA_G
Cuts at: 826 1045 2520 2695 3212 3523 4049 4493 4555
Size: 219 1475 175 517 311 526 444 62
Cuts at: 4555 4843 5978 6659 6850 826
Size: 288 1135 681 191 1471
Fragments arranged by size:
1475 1471 1135 681 526 517 444 311
288 219 191 175 62
SfiI GGCCn_nnn'nGGCC
Cuts at: 3258 3258
Size: 7495
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7495
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7495
SmaI CCC'GGG
Cuts at: 1872 2773 3315 4114 4822 1872
Size: 901 542 799 708 4545
Fragments arranged by size:
4545 901 799 708 542
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2183 2254 2638 2665 3755 4684
Size: 197 926 240 71 384 27 1090 929
Cuts at: 4684 5599 6500 6762 820
Size: 915 901 262 1553
Fragments arranged by size:
1553 1090 929 926 915 901 384 262
240 197 71 27
SnaBI TAC'GTA
Cuts at: 493 5286 493
Size: 4793 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7495
SphI G_CATG'C
Cuts at: 1132 2529 3190 4853 1132
Size: 1397 661 1663 3774
Fragments arranged by size:
3774 1663 1397 661
SspI AAT'ATT
Cuts at: 5 52 5419 5
Size: 47 5367 2081
Fragments arranged by size:
5367 2081 47
StuI AGG'CCT
Cuts at: 1517 3138 3222 3734 3824 1517
Size: 1621 84 512 90 5188
Fragments arranged by size:
5188 1621 512 90 84
StyI C'CwwG_G
Cuts at: 513 1065 2063 2982 3370 4474 4599 4934 513
Size: 552 998 919 388 1104 125 335 3074
Fragments arranged by size:
3074 1104 998 919 552 388 335 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2321 3308 5522 5539 5692 5877 6322
Size: 1255 741 987 2214 17 153 185 445
Cuts at: 6322 325
Size: 1498
Fragments arranged by size:
2214 1498 1255 987 741 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4231 5741 7453
Size: 274 80 33 51 1443 2254 1510 1712
Cuts at: 7453 96
Size: 138
Fragments arranged by size:
2254 1712 1510 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2059 2091 2131 2163 2449 2481 4238 4270 4406
Size: 32 40 32 286 32 1757 32 136
Cuts at: 4406 4438 7455 7487 2059
Size: 32 3017 32 2067
Fragments arranged by size:
3017 2067 1757 286 136 40 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7495
XbaI T'CTAG_A
Cuts at: 4831 4831
Size: 7495
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2983 4481 2983
Size: 1498 5997
XhoI C'TCGA_G
Cuts at: 1943 4684 1943
Size: 2741 4754
XmaI C'CCGG_G
Cuts at: 1870 2771 3313 4112 4820 1870
Size: 901 542 799 708 4545
Fragments arranged by size:
4545 901 799 708 542
XmnI GAAnn'nnTTC
Cuts at: 3824 5624 3824
Size: 1800 5695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5303 276
Size: 53 83 186 936 3769 2468
Fragments arranged by size:
3769 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I EcoRV KasI NarI NruI NsiI PacI
PmlI PsrI RsrII SacII SanDI SapI SfoI SrfI
SwaI