Restriction map for
pFN21AE2108
(Circular) (Six-base) MAPSORT of: pFN21AE2108.seq Check: 2597 from: 1 to: 7240
pFN21AE2108 7240 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2492 2492
Size: 7240
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 5050 278
Size: 53 83 186 936 2245 1269 2468
Fragments arranged by size:
2468 2245 1269 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3514 3975 4471 4583 1937
Size: 1577 461 496 112 4594
Fragments arranged by size:
4594 1577 496 461 112
Acc65I G'GTAC_C
Cuts at: 4561 4561
Size: 7240
AclI AA'CG_TT
Cuts at: 1563 5367 5740 1563
Size: 3804 373 3063
Fragments arranged by size:
3804 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3469 4464 5303 6687 6955 1632
Size: 1837 995 839 1384 268 1917
Fragments arranged by size:
1917 1837 1384 995 839 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7043
AflIII A'CryG_T
Cuts at: 1203 2216 2462 3517 3870 4494 6139 7116 1203
Size: 1013 246 1055 353 624 1645 977 1327
Fragments arranged by size:
1645 1327 1055 1013 977 624 353 246
AgeI A'CCGG_T
Cuts at: 6071 6071
Size: 7240
AhdI GACnn_n'nnGTC
Cuts at: 3700 5969 3700
Size: 2269 4971
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7240
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7240
AlwNI CAG_nnn'CTG
Cuts at: 3042 4983 6555 3042
Size: 1941 1572 3727
Fragments arranged by size:
3727 1941 1572
ApaI G_GGCC'C
Cuts at: 2966 2966
Size: 7240
ApaLI G'TGCA_C
Cuts at: 2122 5297 6453 2122
Size: 3175 1156 2909
Fragments arranged by size:
3175 2909 1156
ApoI r'AATT_y
Cuts at: 1493 2972 3228 4019 4549 4775 4927 7036 1493
Size: 1479 256 791 530 226 152 2109 1697
Fragments arranged by size:
2109 1697 1479 791 530 256 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4179 5794 160
Size: 872 3147 1615 1606
Fragments arranged by size:
3147 1615 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7240
AvaI C'yCGr_G
Cuts at: 1870 1943 2061 4565 1870
Size: 73 118 2504 4545
Fragments arranged by size:
4545 2504 118 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4260 4293 4481 4514 4260
Size: 33 188 33 6986
Fragments arranged by size:
6986 188 33 33
BamHI G'GATC_C
Cuts at: 4570 4937 4570
Size: 367 6873
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 2072 2366 2585 4010
Size: 325 217 627 269 16 294 219 1425
Cuts at: 4010 4561 6016 618
Size: 551 1455 1842
Fragments arranged by size:
1842 1455 1425 627 551 325 294 269
219 217 16
BanII G_rGCy'C
Cuts at: 1391 2966 3057 4559 1391
Size: 1575 91 1502 4072
Fragments arranged by size:
4072 1575 1502 91
BbeI G_GCGC'C
Cuts at: 2060 2076 2370 2589 2060
Size: 16 294 219 6711
Fragments arranged by size:
6711 294 219 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2178 2219 4134 928
Size: 971 279 41 1915 4034
Fragments arranged by size:
4034 1915 971 279 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2630 2664 5431 5465 1903
Size: 34 693 34 2767 34 3678
Fragments arranged by size:
3678 2767 693 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2396 2912 5132 6342 2396
Size: 516 2220 1210 3294
Fragments arranged by size:
3294 2220 1210 516
BclI T'GATC_A
Cuts at: 1548 4183 1548
Size: 2635 4605
BfrBI ATG'CAT
Cuts at: 2997 2997
Size: 7240
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4319 5851 136
Size: 107 122 71 631 605 2647 1532 1525
Fragments arranged by size:
2647 1532 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7235 7235
Size: 7240
BlpI GC'TnA_GC
Cuts at: 4657 4657
Size: 7240
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2126 2572 2966 5301 6457 1790
Size: 222 114 446 394 2335 1156 2573
Fragments arranged by size:
2573 2335 1156 446 394 222 114
BmgBI CAC'GTC
Cuts at: 1318 2013 2217 1318
Size: 695 204 6341
Fragments arranged by size:
6341 695 204
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2384 3736 4209
Size: 416 53 83 399 24 967 1352 473
Cuts at: 4209 4971 5929 442
Size: 762 958 1753
Fragments arranged by size:
1753 1352 967 958 762 473 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4040 1056
Size: 2984 4256
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3697 4471 3697
Size: 774 6466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2419 2473 2491 2702 3064 3947 5900 1239
Size: 1180 54 18 211 362 883 1953 2579
Fragments arranged by size:
2579 1953 1180 883 362 211 54 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5365 6230 6528 5365
Size: 865 298 6077
Fragments arranged by size:
6077 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5903 882
Size: 617 4404 2219
Fragments arranged by size:
4404 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4497 5031 493
Size: 711 3293 534 2702
Fragments arranged by size:
3293 2702 711 534
BsaBI GATnn'nnATC
Cuts at: 2283 4530 7234 2283
Size: 2247 2704 2289
Fragments arranged by size:
2704 2289 2247
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2057 2073 2367 2586
Size: 53 83 186 936 524 16 294 219
Cuts at: 2586 3778 5047 5429 6064 275
Size: 1192 1269 382 635 1451
Fragments arranged by size:
1451 1269 1192 936 635 524 382 294
219 186 83 53 16
BsaWI w'CCGG_w
Cuts at: 2078 5673 6071 6345 6492 6903 2078
Size: 3595 398 274 147 411 2415
Fragments arranged by size:
3595 2415 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7015
Fragments arranged by size:
7015 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2602 1186
Size: 1416 5824
BseYI C'CCAG_C
Cuts at: 2052 2261 2631 3155 4501 6443 6939 2052
Size: 209 370 524 1346 1942 496 2353
Fragments arranged by size:
2353 1942 1346 524 496 370 209
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2623 3001 3171 1163
Size: 573 887 378 170 5232
Fragments arranged by size:
5232 887 573 378 170
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4690 5451 6479 1540
Size: 275 186 2689 761 1028 2301
Fragments arranged by size:
2689 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2126 5301 5386 6457 2126
Size: 3175 85 1071 2909
Fragments arranged by size:
3175 2909 1071 85
BsmI GAATG_Cn'
Cuts at: 2999 3086 3206 4751 4844 2999
Size: 87 120 1545 93 5395
Fragments arranged by size:
5395 1545 120 93 87
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2126 2572 2966 3057 4559 5301
Size: 399 222 114 446 394 91 1502 742
Cuts at: 5301 5386 6457 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1502 1071 742 446 399 394 222
114 91 85
BspEI T'CCGG_A
Cuts at: 6903 6903
Size: 7240
BspHI T'CATG_A
Cuts at: 4087 5129 6859 4087
Size: 1042 1730 4468
Fragments arranged by size:
4468 1730 1042
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2492 4595 844
Size: 1648 2103 3489
Fragments arranged by size:
3489 2103 1648
BsrBI CCG'CTC
Cuts at: 1394 1823 2070 5127 1394
Size: 429 247 3057 3507
Fragments arranged by size:
3507 3057 429 247
BsrDI GCAATG_nn'
Cuts at: 65 1248 2530 3435 3555 5735 5909 65
Size: 1183 1282 905 120 2180 174 1396
Fragments arranged by size:
2180 1396 1282 1183 905 174 120
BsrFI r'CCGG_y
Cuts at: 1700 1810 5884 6071 1700
Size: 110 4074 187 2869
Fragments arranged by size:
4074 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3573 4096 96
Size: 3477 523 3240
Fragments arranged by size:
3477 3240 523
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7240
BssSI C'ACGA_G
Cuts at: 5300 6312 5300
Size: 1012 6228
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3557 794
Size: 2763 4477
BstBI TT'CG_AA
Cuts at: 6044 6044
Size: 7240
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7240
BstXI CCAn_nnnn'nTGG
Cuts at: 2397 3691 2397
Size: 1294 5946
BstYI r'GATC_y
Cuts at: 1972 2284 3286 4570 4937 5322 5339 6791 6877
Size: 312 1002 1284 367 385 17 1452 86
Cuts at: 6877 6889 7235 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1284 1002 385 367 346 312
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2878 513
Size: 552 939 874 4875
Fragments arranged by size:
4875 939 874 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2559 2788 487
Size: 683 56 1333 229 4939
Fragments arranged by size:
4939 1333 683 229 56
BtsI GCAGTG_nn'
Cuts at: 2194 2557 2693 2812 2897 4756 5550 5570 2194
Size: 363 136 119 85 1859 794 20 3864
Fragments arranged by size:
3864 1859 794 363 136 119 85 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7240
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7205
DraI TTT'AAA
Cuts at: 4544 4544
Size: 7240
DraIII CAC_nnn'GTG
Cuts at: 2718 2718
Size: 7240
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4079 6247 809
Size: 507 2763 2168 1802
Fragments arranged by size:
2763 2168 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2248 2494 2527
Size: 54 1425 50 275 18 418 246 33
Cuts at: 2527 2692 3895 4240 4687 5576 7205 8
Size: 165 1203 345 447 889 1629 43
Fragments arranged by size:
1629 1425 1203 889 447 418 345 275
246 165 54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 4687 1537
Size: 275 2875 4090
Fragments arranged by size:
4090 2875 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3343 4440 5170 1353
Size: 1990 1097 730 3423
Fragments arranged by size:
3423 1990 1097 730
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4393 5827 6201 6347 387
Size: 1064 138 2804 1434 374 146 1280
Fragments arranged by size:
2804 1434 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2419 2473 2491 2702 3064 3469 3947
Size: 393 787 54 18 211 362 405 478
Cuts at: 3947 4464 5303 5900 6687 6955 1239
Size: 517 839 597 787 268 1524
Fragments arranged by size:
1524 839 787 787 597 517 478 405
393 362 268 211 54 18
EcoO109I rG'GnC_Cy
Cuts at: 2785 2963 4450 2785
Size: 178 1487 5575
Fragments arranged by size:
5575 1487 178
EcoRI G'AATT_C
Cuts at: 4549 4927 4549
Size: 378 6862
EcoRV GAT'ATC
Cuts at: 3004 3799 3004
Size: 795 6445
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3385 3499 3385
Size: 114 7126
FspI TGC'GCA
Cuts at: 5746 5746
Size: 7240
HaeII r_GCGC'y
Cuts at: 1678 2030 2060 2076 2370 2589 6387 1678
Size: 352 30 16 294 219 3798 2531
Fragments arranged by size:
3798 2531 352 294 219 30 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2898 2930 3686 3718 4168
Size: 32 135 32 1326 32 756 32 450
Cuts at: 4168 4200 5881 5913 5955 5987 1373
Size: 32 1681 32 42 32 2626
Fragments arranged by size:
2626 1681 1326 756 450 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2182 2818 2935 4051 4584 4830
Size: 988 281 244 636 117 1116 533 246
Cuts at: 4830 669
Size: 3079
Fragments arranged by size:
3079 1116 988 636 533 281 246 244
117
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7240
HpaI GTT'AAC
Cuts at: 4830 4830
Size: 7240
KasI G'GCGC_C
Cuts at: 2056 2072 2366 2585 2056
Size: 16 294 219 6711
Fragments arranged by size:
6711 294 219 16
KpnI G_GTAC'C
Cuts at: 4565 4565
Size: 7240
MfeI C'AATT_G
Cuts at: 3495 4839 3495
Size: 1344 5896
MluI A'CGCG_T
Cuts at: 7116 7116
Size: 7240
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2685 3415 4468 6354 6538 1305
Size: 1380 730 1053 1886 184 2007
Fragments arranged by size:
2007 1886 1380 1053 730 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2250 2529 3897 10
Size: 54 1425 343 418 279 1368 3353
Fragments arranged by size:
3353 1425 1368 418 343 279 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3689 5198 5557 5716 518
Size: 648 2523 1509 359 159 2042
Fragments arranged by size:
2523 2042 1509 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2361 2499 2631 2871 3155 3794 4656 5333
Size: 755 138 132 240 284 639 862 677
Cuts at: 5333 6481 6726 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 862 755 677 639 284 245
240 138 132
NarI GG'CG_CC
Cuts at: 2057 2073 2367 2586 2057
Size: 16 294 219 6711
Fragments arranged by size:
6711 294 219 16
NcoI C'CATG_G
Cuts at: 513 1065 2004 2878 513
Size: 552 939 874 4875
Fragments arranged by size:
4875 939 874 552
NdeI CA'TA_TG
Cuts at: 387 2331 387
Size: 1944 5296
NheI G'CTAG_C
Cuts at: 1052 4036 1052
Size: 2984 4256
NotI GC'GGCC_GC
Cuts at: 4687 4687
Size: 7240
NsiI A_TGCA'T
Cuts at: 2999 2999
Size: 7240
NspI r_CATG'y
Cuts at: 1132 1229 2343 2466 3521 3874 4598 6143 1132
Size: 97 1114 123 1055 353 724 1545 2229
Fragments arranged by size:
2229 1545 1114 1055 724 353 123 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7240
PciI A'CATG_T
Cuts at: 2462 3517 3870 6139 2462
Size: 1055 353 2269 3563
Fragments arranged by size:
3563 2269 1055 353
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2268 2582 2631 3588 3704 1108
Size: 1160 314 49 957 116 4644
Fragments arranged by size:
4644 1160 957 314 116 49
PfoI T'CCnGG_A
Cuts at: 2396 2507 3211 2396
Size: 111 704 6425
Fragments arranged by size:
6425 704 111
PmeI GTTT'AAAC
Cuts at: 4544 4544
Size: 7240
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5309 5341 6822 6854 5309
Size: 32 1481 32 5695
Fragments arranged by size:
5695 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2785 4450 2785
Size: 1665 5575
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7240
PsiI TTA'TAA
Cuts at: 3833 4810 3833
Size: 977 6263
PspOMI G'GGCC_C
Cuts at: 2962 2962
Size: 7240
PstI C_TGCA'G
Cuts at: 830 2543 3038 3224 3553 4592 830
Size: 1713 495 186 329 1039 3478
Fragments arranged by size:
3478 1713 1039 495 329 186
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7240
PvuII CAG'CTG
Cuts at: 2361 2631 2871 3155 2361
Size: 270 240 284 6446
Fragments arranged by size:
6446 284 270 240
SalI G'TCGA_C
Cuts at: 1936 4582 1936
Size: 2646 4594
SanDI GG'GwC_CC
Cuts at: 2785 2785
Size: 7240
SbfI CC_TGCA'GG
Cuts at: 4592 4592
Size: 7240
ScaI AGT'ACT
Cuts at: 3915 5488 7200 3915
Size: 1573 1712 3955
Fragments arranged by size:
3955 1712 1573
SfcI C'TryA_G
Cuts at: 826 1045 2539 3034 3220 3549 4588 5723 6404
Size: 219 1494 495 186 329 1039 1135 681
Cuts at: 6404 6595 826
Size: 191 1471
Fragments arranged by size:
1494 1471 1135 1039 681 495 329 219
191 186
SfoI GGC'GCC
Cuts at: 2058 2074 2368 2587 2058
Size: 16 294 219 6711
Fragments arranged by size:
6711 294 219 16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7240
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7240
SmaI CCC'GGG
Cuts at: 1872 2063 4567 1872
Size: 191 2504 4545
Fragments arranged by size:
4545 2504 191
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5344 6245 6507 820
Size: 197 926 3401 901 262 1553
Fragments arranged by size:
3401 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5031 493
Size: 4538 2702
SpeI A'CTAG_T
Cuts at: 152 3394 152
Size: 3242 3998
SphI G_CATG'C
Cuts at: 1132 4598 1132
Size: 3466 3774
SspI AAT'ATT
Cuts at: 5 52 3960 5164 5
Size: 47 3908 1204 2081
Fragments arranged by size:
3908 2081 1204 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7240
StyI C'CwwG_G
Cuts at: 513 1065 2004 2878 4679 513
Size: 552 939 874 1801 3074
Fragments arranged by size:
3074 1801 939 874 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3559 5267 5284 5437 5622 6067 325
Size: 1255 1979 1708 17 153 185 445 1498
Fragments arranged by size:
1979 1708 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3573 3913 4096
Size: 274 80 33 51 1443 1596 340 183
Cuts at: 4096 4428 5486 7198 96
Size: 332 1058 1712 138
Fragments arranged by size:
1712 1596 1443 1058 340 332 274 183
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2704 2736 3169 3201 3788 3820 3860 3892 7200
Size: 32 433 32 587 32 40 32 3308
Cuts at: 7200 7232 2704
Size: 32 2712
Fragments arranged by size:
3308 2712 587 433 40 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2912 3536 1316
Size: 1596 624 5020
Fragments arranged by size:
5020 1596 624
XbaI T'CTAG_A
Cuts at: 4576 4576
Size: 7240
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2126 2744 2126
Size: 618 6622
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7240
XmaI C'CCGG_G
Cuts at: 1870 2061 4565 1870
Size: 191 2504 4545
Fragments arranged by size:
4545 2504 191
XmnI GAAnn'nnTTC
Cuts at: 3452 3767 5369 3452
Size: 315 1602 5323
Fragments arranged by size:
5323 1602 315
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 5048 276
Size: 53 83 186 936 2245 1269 2468
Fragments arranged by size:
2468 2245 1269 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbvCI Bpu10I BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI NaeI NgoMIV
NruI PacI PmlI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SrfI SwaI