Restriction map for
pFN21AE2105
(Circular) (Six-base) MAPSORT of: pFN21AE2105.seq Check: 5048 from: 1 to: 7222
pFN21AE2105 7222 bp
With 182 enzymes: SgfI *
March 7, 2011 13:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2236 4339 2236
Size: 2103 5119
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5032 278
Size: 53 83 186 936 3496 2468
Fragments arranged by size:
3496 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4565 1937
Size: 2628 4594
Acc65I G'GTAC_C
Cuts at: 4543 4543
Size: 7222
AclI AA'CG_TT
Cuts at: 1563 5349 5722 1563
Size: 3786 373 3063
Fragments arranged by size:
3786 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2212 3554 3825 5285 6669 6937 1632
Size: 580 1342 271 1460 1384 268 1917
Fragments arranged by size:
1917 1460 1384 1342 580 271 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7025
AflIII A'CryG_T
Cuts at: 1203 6121 7098 1203
Size: 4918 977 1327
Fragments arranged by size:
4918 1327 977
AgeI A'CCGG_T
Cuts at: 6053 6053
Size: 7222
AhdI GACnn_n'nnGTC
Cuts at: 5951 5951
Size: 7222
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2603 3878 1184
Size: 1419 1275 4528
Fragments arranged by size:
4528 1419 1275
AlwNI CAG_nnn'CTG
Cuts at: 2251 2395 4282 4965 6537 2251
Size: 144 1887 683 1572 2936
Fragments arranged by size:
2936 1887 1572 683 144
ApaLI G'TGCA_C
Cuts at: 5279 6435 5279
Size: 1156 6066
ApoI r'AATT_y
Cuts at: 1493 2134 2789 2864 3026 4087 4531 4757 4909
Size: 641 655 75 162 1061 444 226 152
Cuts at: 4909 7018 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1061 655 641 444 226 162
152 75
AseI AT'TA_AT
Cuts at: 160 1032 5776 160
Size: 872 4744 1606
Fragments arranged by size:
4744 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7222
AvaI C'yCGr_G
Cuts at: 1870 1943 4547 1870
Size: 73 2604 4545
Fragments arranged by size:
4545 2604 73
BamHI G'GATC_C
Cuts at: 4552 4919 4552
Size: 367 6855
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4102 4543 5998 618
Size: 325 217 627 2315 441 1455 1842
Fragments arranged by size:
2315 1842 1455 627 441 325 217
BanII G_rGCy'C
Cuts at: 1391 2088 4541 1391
Size: 697 2453 4072
Fragments arranged by size:
4072 2453 697
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3560 4337 928
Size: 971 1661 777 3813
Fragments arranged by size:
3813 1661 971 777
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5413 5447 1903
Size: 34 3476 34 3678
Fragments arranged by size:
3678 3476 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3604 5114 6324 3604
Size: 1510 1210 4502
Fragments arranged by size:
4502 1510 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7222
BfrBI ATG'CAT
Cuts at: 2124 2124
Size: 7222
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5833 136
Size: 107 122 71 631 605 4161 1525
Fragments arranged by size:
4161 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3139 3217 7217 3139
Size: 78 4000 3144
Fragments arranged by size:
4000 3144 78
BlpI GC'TnA_GC
Cuts at: 4639 4639
Size: 7222
Bme1580I G_kGCm'C
Cuts at: 1790 5283 6439 1790
Size: 3493 1156 2573
Fragments arranged by size:
3493 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7222
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4072 4953 5911
Size: 416 53 83 399 24 2655 881 958
Cuts at: 5911 442
Size: 1753
Fragments arranged by size:
2655 1753 958 881 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3547 3548 3626 4409 4447 5882 1239
Size: 2308 1 78 783 38 1435 2579
Fragments arranged by size:
2579 2308 1435 783 78 38 1
Bpu10I CC'TnA_GC
Cuts at: 2186 2306 4340 2186
Size: 120 2034 5068
Fragments arranged by size:
5068 2034 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3149 3178 4030 5347 6212 6510 3149
Size: 29 852 1317 865 298 3861
Fragments arranged by size:
3861 1317 865 852 298 29
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2062 5885 882
Size: 617 563 3823 2219
Fragments arranged by size:
3823 2219 617 563
BsaAI yAC'GTr
Cuts at: 493 1204 5013 493
Size: 711 3809 2702
Fragments arranged by size:
3809 2702 711
BsaBI GATnn'nnATC
Cuts at: 7216 7216
Size: 7222
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5029 5411 6046 275
Size: 53 83 186 936 3496 382 635 1451
Fragments arranged by size:
3496 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5655 6053 6327 6474 6885 5655
Size: 398 274 147 411 5992
Fragments arranged by size:
5992 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3521 3545 3551 3575 1178
Size: 30 165 30 2118 24 6 24 4825
Fragments arranged by size:
4825 2118 165 30 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7222
BseYI C'CCAG_C
Cuts at: 2027 2041 2079 4218 6425 6921 2027
Size: 14 38 2139 2207 496 2328
Fragments arranged by size:
2328 2207 2139 496 38 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6649
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4672 5433 6461 1540
Size: 275 186 2671 761 1028 2301
Fragments arranged by size:
2671 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2541 5283 5368 6439 2541
Size: 2742 85 1071 3324
Fragments arranged by size:
3324 2742 1071 85
BsmI GAATG_Cn'
Cuts at: 3103 3295 4733 4826 3103
Size: 192 1438 93 5499
Fragments arranged by size:
5499 1438 192 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4423 4423
Size: 7222
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2541 4541 5283 5368 6439 1391
Size: 399 298 453 2000 742 85 1071 2174
Fragments arranged by size:
2174 2000 1071 742 453 399 298 85
BspEI T'CCGG_A
Cuts at: 6885 6885
Size: 7222
BspHI T'CATG_A
Cuts at: 5111 6841 5111
Size: 1730 5492
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2236 4339 4577 844
Size: 1392 2103 238 3489
Fragments arranged by size:
3489 2103 1392 238
BsrBI CCG'CTC
Cuts at: 1394 1823 5109 1394
Size: 429 3286 3507
Fragments arranged by size:
3507 3286 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3785 4155 5717 5891 65
Size: 1183 2537 370 1562 174 1396
Fragments arranged by size:
2537 1562 1396 1183 370 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5866 6053 1700
Size: 110 4056 187 2869
Fragments arranged by size:
4056 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7222
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7222
BssSI C'ACGA_G
Cuts at: 5282 6294 5282
Size: 1012 6210
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3856 794
Size: 3062 4160
BstBI TT'CG_AA
Cuts at: 6026 6026
Size: 7222
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7222
BstXI CCAn_nnnn'nTGG
Cuts at: 2079 2179 2079
Size: 100 7122
BstYI r'GATC_y
Cuts at: 1972 3139 3217 4552 4919 5304 5321 6773 6859
Size: 1167 78 1335 367 385 17 1452 86
Cuts at: 6859 6871 7217 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1335 1167 385 367 346 86
78 17 12
Bsu36I CC'TnA_GG
Cuts at: 2201 2201
Size: 7222
BtgI C'CryG_G
Cuts at: 513 1065 2046 513
Size: 552 981 5689
Fragments arranged by size:
5689 981 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6483
Fragments arranged by size:
6483 683 56
BtsI GCAGTG_nn'
Cuts at: 4738 5532 5552 4738
Size: 794 20 6408
Fragments arranged by size:
6408 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7222
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3073 3108 529
Size: 35 2509 35 4643
Fragments arranged by size:
4643 2509 35 35
DraI TTT'AAA
Cuts at: 2674 2724 4526 2674
Size: 50 1802 5370
Fragments arranged by size:
5370 1802 50
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6229 809
Size: 507 4913 1802
Fragments arranged by size:
4913 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4152 4669 5558
Size: 54 1425 50 275 18 2322 517 889
Cuts at: 5558 7187 8
Size: 1629 43
Fragments arranged by size:
2322 1629 1425 889 517 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4669 1537
Size: 275 2857 4090
Fragments arranged by size:
4090 2857 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5152 1353
Size: 3799 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5809 6183 6329 387
Size: 1064 138 4220 374 146 1280
Fragments arranged by size:
4220 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 3547 3548 3554 3626 3825 4409
Size: 393 580 1335 1 6 72 199 584
Cuts at: 4409 4447 5285 5882 6669 6937 1239
Size: 38 838 597 787 268 1524
Fragments arranged by size:
1524 1335 838 787 597 584 580 393
268 199 72 38 6 1
EcoNI CCTnn'n_nnAGG
Cuts at: 4319 4319
Size: 7222
EcoO109I rG'GnC_Cy
Cuts at: 2839 2839
Size: 7222
EcoRI G'AATT_C
Cuts at: 2864 4531 4909 2864
Size: 1667 378 5177
Fragments arranged by size:
5177 1667 378
EcoRV GAT'ATC
Cuts at: 3475 3475
Size: 7222
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3090 3090
Size: 7222
FspI TGC'GCA
Cuts at: 5728 5728
Size: 7222
HaeII r_GCGC'y
Cuts at: 1678 6369 1678
Size: 4691 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2179 2211 3521 3553 4445
Size: 32 135 32 607 32 1310 32 892
Cuts at: 4445 4477 5863 5895 5937 5969 1373
Size: 32 1386 32 42 32 2626
Fragments arranged by size:
2626 1386 1310 892 607 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3019 3524 4566 4812 669
Size: 988 281 1081 505 1042 246 3079
Fragments arranged by size:
3079 1081 1042 988 505 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7222
HpaI GTT'AAC
Cuts at: 3019 4812 3019
Size: 1793 5429
KpnI G_GTAC'C
Cuts at: 4547 4547
Size: 7222
MfeI C'AATT_G
Cuts at: 2280 4498 4821 2280
Size: 2218 323 4681
Fragments arranged by size:
4681 2218 323
MluI A'CGCG_T
Cuts at: 7098 7098
Size: 7222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2683 3108 3566 6336 6520 1305
Size: 1378 425 458 2770 184 2007
Fragments arranged by size:
2770 2007 1378 458 425 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4154 10
Size: 54 1425 343 2322 3078
Fragments arranged by size:
3078 2322 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3860 3908 5180 5539 5698 518
Size: 648 2694 48 1272 359 159 2042
Fragments arranged by size:
2694 2042 1272 648 359 159 48
MspA1I CmG'CkG
Cuts at: 1606 4239 4638 5315 6463 6708 1606
Size: 2633 399 677 1148 245 2120
Fragments arranged by size:
2633 2120 1148 677 399 245
NcoI C'CATG_G
Cuts at: 513 1065 2046 513
Size: 552 981 5689
Fragments arranged by size:
5689 981 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7222
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7222
NotI GC'GGCC_GC
Cuts at: 4669 4669
Size: 7222
NsiI A_TGCA'T
Cuts at: 2126 2126
Size: 7222
NspI r_CATG'y
Cuts at: 1132 1229 3859 4580 6125 1132
Size: 97 2630 721 1545 2229
Fragments arranged by size:
2630 2229 1545 721 97
PasI CC'CwG_GG
Cuts at: 1348 2359 1348
Size: 1011 6211
PciI A'CATG_T
Cuts at: 6121 6121
Size: 7222
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2776 1108
Size: 1668 5554
PfoI T'CCnGG_A
Cuts at: 3453 3504 3453
Size: 51 7171
PmeI GTTT'AAAC
Cuts at: 4526 4526
Size: 7222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5291 5323 6804 6836 5291
Size: 32 1481 32 5677
Fragments arranged by size:
5677 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7222
PsiI TTA'TAA
Cuts at: 2988 3624 4792 2988
Size: 636 1168 5418
Fragments arranged by size:
5418 1168 636
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4471 4503 4471
Size: 32 7190
PstI C_TGCA'G
Cuts at: 830 2247 4320 4574 830
Size: 1417 2073 254 3478
Fragments arranged by size:
3478 2073 1417 254
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7222
SalI G'TCGA_C
Cuts at: 1936 4564 1936
Size: 2628 4594
SbfI CC_TGCA'GG
Cuts at: 4574 4574
Size: 7222
ScaI AGT'ACT
Cuts at: 5470 7182 5470
Size: 1712 5510
SexAI A'CCwGG_T
Cuts at: 2662 2662
Size: 7222
SfcI C'TryA_G
Cuts at: 826 1045 2218 2243 3695 4129 4316 4570 5705
Size: 219 1173 25 1452 434 187 254 1135
Cuts at: 5705 6386 6577 826
Size: 681 191 1471
Fragments arranged by size:
1471 1452 1173 1135 681 434 254 219
191 187 25
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7222
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7222
SmaI CCC'GGG
Cuts at: 1872 4549 1872
Size: 2677 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3128 3157 4045 5326 6227 6489
Size: 197 926 1185 29 888 1281 901 262
Cuts at: 6489 820
Size: 1553
Fragments arranged by size:
1553 1281 1185 926 901 888 262 197
29
SnaBI TAC'GTA
Cuts at: 493 5013 493
Size: 4520 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7222
SphI G_CATG'C
Cuts at: 1132 3859 4580 1132
Size: 2727 721 3774
Fragments arranged by size:
3774 2727 721
SspI AAT'ATT
Cuts at: 5 52 2110 2328 5146 5
Size: 47 2058 218 2818 2081
Fragments arranged by size:
2818 2081 2058 218 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7222
StyI C'CwwG_G
Cuts at: 513 1065 2046 4661 513
Size: 552 981 2615 3074
Fragments arranged by size:
3074 2615 981 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3904 5249 5266 5419 5604 6049 325
Size: 1255 2324 1345 17 153 185 445 1498
Fragments arranged by size:
2324 1498 1345 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3752 5468 7180
Size: 274 80 33 51 1443 1775 1716 1712
Cuts at: 7180 96
Size: 138
Fragments arranged by size:
1775 1716 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7182 7214 7182
Size: 32 7190
Tth111I GACn'n_nGTC
Cuts at: 1316 4403 1316
Size: 3087 4135
XbaI T'CTAG_A
Cuts at: 2425 2533 2833 3136 4558 2425
Size: 108 300 303 1422 5089
Fragments arranged by size:
5089 1422 303 300 108
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7222
XmaI C'CCGG_G
Cuts at: 1870 4547 1870
Size: 2677 4545
XmnI GAAnn'nnTTC
Cuts at: 5351 5351
Size: 7222
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5030 276
Size: 53 83 186 936 3496 2468
Fragments arranged by size:
3496 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstZ17I DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI
PspXI PvuII RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI XcmI