Restriction map for
pFN21AE2097
(Circular) (Six-base) MAPSORT of: pFN21AE2097.seq Check: 3605 from: 1 to: 7120
pFN21AE2097 7120 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4930 278
Size: 53 83 186 936 3394 2468
Fragments arranged by size:
3394 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4463 1937
Size: 2526 4594
Acc65I G'GTAC_C
Cuts at: 4441 4441
Size: 7120
AclI AA'CG_TT
Cuts at: 1563 5247 5620 1563
Size: 3684 373 3063
Fragments arranged by size:
3684 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2190 2273 2288 2304 3055 4119 5183 6567
Size: 558 83 15 16 751 1064 1064 1384
Cuts at: 6567 6835 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1064 1064 751 558 268 83
16 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6923
AflIII A'CryG_T
Cuts at: 1203 3366 6019 6996 1203
Size: 2163 2653 977 1327
Fragments arranged by size:
2653 2163 1327 977
AgeI A'CCGG_T
Cuts at: 5951 5951
Size: 7120
AhdI GACnn_n'nnGTC
Cuts at: 2209 5849 2209
Size: 3640 3480
AleI CACnn'nnGTG
Cuts at: 1166 3092 3627 1166
Size: 1926 535 4659
Fragments arranged by size:
4659 1926 535
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7120
AlwNI CAG_nnn'CTG
Cuts at: 3653 3664 4863 6435 3653
Size: 11 1199 1572 4338
Fragments arranged by size:
4338 1572 1199 11
ApaLI G'TGCA_C
Cuts at: 3085 5177 6333 3085
Size: 2092 1156 3872
Fragments arranged by size:
3872 2092 1156
ApoI r'AATT_y
Cuts at: 1493 2265 2992 3405 3805 4429 4655 4807 6916
Size: 772 727 413 400 624 226 152 2109
Cuts at: 6916 1493
Size: 1697
Fragments arranged by size:
2109 1697 772 727 624 413 400 226
152
AseI AT'TA_AT
Cuts at: 160 1032 5674 160
Size: 872 4642 1606
Fragments arranged by size:
4642 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7120
AvaI C'yCGr_G
Cuts at: 1870 1943 4445 1870
Size: 73 2502 4545
Fragments arranged by size:
4545 2502 73
BamHI G'GATC_C
Cuts at: 3075 4450 4817 3075
Size: 1375 367 5378
Fragments arranged by size:
5378 1375 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4441 5896 618
Size: 325 217 627 2654 1455 1842
Fragments arranged by size:
2654 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3774 4439 1391
Size: 2383 665 4072
Fragments arranged by size:
4072 2383 665
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2121 2310 2340 2954 3263 3300 928
Size: 971 222 189 30 614 309 37 4748
Fragments arranged by size:
4748 971 614 309 222 189 37 30
BbvCI CC'TCA_GC
Cuts at: 2531 3677 2531
Size: 1146 5974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5311 5345 1903
Size: 34 3374 34 3678
Fragments arranged by size:
3678 3374 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2262 4176 5012 6222 2262
Size: 1914 836 1210 3160
Fragments arranged by size:
3160 1914 1210 836
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7120
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5731 136
Size: 107 122 71 631 605 4059 1525
Fragments arranged by size:
4059 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2510 7115 2510
Size: 4605 2515
BlpI GC'TnA_GC
Cuts at: 4537 4537
Size: 7120
Bme1580I G_kGCm'C
Cuts at: 1790 3089 3480 5181 6337 1790
Size: 1299 391 1701 1156 2573
Fragments arranged by size:
2573 1701 1299 1156 391
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7120
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4302 4851 5809
Size: 416 53 83 399 24 2885 549 958
Cuts at: 5809 442
Size: 1753
Fragments arranged by size:
2885 1753 958 549 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2527 3853 5780 1239
Size: 1288 1326 1927 2579
Fragments arranged by size:
2579 1927 1326 1288
Bpu10I CC'TnA_GC
Cuts at: 2531 3249 3677 2531
Size: 718 428 5974
Fragments arranged by size:
5974 718 428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2961 5245 6110 6408 2961
Size: 2284 865 298 3673
Fragments arranged by size:
3673 2284 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5783 882
Size: 617 4284 2219
Fragments arranged by size:
4284 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4911 493
Size: 711 3707 2702
Fragments arranged by size:
3707 2702 711
BsaBI GATnn'nnATC
Cuts at: 7114 7114
Size: 7120
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4927 5309 5944 275
Size: 53 83 186 936 3394 382 635 1451
Fragments arranged by size:
3394 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5553 5951 6225 6372 6783 5553
Size: 398 274 147 411 5890
Fragments arranged by size:
5890 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4248 4278 1178
Size: 30 165 30 2845 30 4020
Fragments arranged by size:
4020 2845 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2631 4019 1186
Size: 1445 1388 4287
Fragments arranged by size:
4287 1445 1388
BseYI C'CCAG_C
Cuts at: 2198 6323 6819 2198
Size: 4125 496 2499
Fragments arranged by size:
4125 2499 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3147 3605 4047 1163
Size: 573 1411 458 442 4236
Fragments arranged by size:
4236 1411 573 458 442
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4570 5331 6359 1540
Size: 275 186 2569 761 1028 2301
Fragments arranged by size:
2569 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2328 2378 3089 5181 5266 6337 2328
Size: 50 711 2092 85 1071 3111
Fragments arranged by size:
3111 2092 1071 711 85 50
BsmI GAATG_Cn'
Cuts at: 2429 2758 4631 4724 2429
Size: 329 1873 93 4825
Fragments arranged by size:
4825 1873 329 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2378 3089 3480 3774 4439 5181
Size: 399 538 50 711 391 294 665 742
Cuts at: 5181 5266 6337 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 711 665 538 399 391
294 85 50
BspEI T'CCGG_A
Cuts at: 6783 6783
Size: 7120
BspHI T'CATG_A
Cuts at: 3124 5009 6739 3124
Size: 1885 1730 3505
Fragments arranged by size:
3505 1885 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4475 844
Size: 3631 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5007 1394
Size: 429 3184 3507
Fragments arranged by size:
3507 3184 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2898 5615 5789 65
Size: 1183 1650 2717 174 1396
Fragments arranged by size:
2717 1650 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5764 5951 1700
Size: 110 3954 187 2869
Fragments arranged by size:
3954 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7120
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7120
BssSI C'ACGA_G
Cuts at: 5180 6192 5180
Size: 1012 6108
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3161 794
Size: 2367 4753
BstBI TT'CG_AA
Cuts at: 5924 5924
Size: 7120
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7120
BstYI r'GATC_y
Cuts at: 1972 2510 3075 4450 4817 5202 5219 6671 6757
Size: 538 565 1375 367 385 17 1452 86
Cuts at: 6757 6769 7115 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1375 565 538 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2659 513
Size: 552 1594 4974
Fragments arranged by size:
4974 1594 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6381
Fragments arranged by size:
6381 683 56
BtsI GCAGTG_nn'
Cuts at: 3752 4059 4636 5430 5450 3752
Size: 307 577 794 20 5422
Fragments arranged by size:
5422 794 577 307 20
ClaI AT'CG_AT
Cuts at: 1554 4356 1554
Size: 2802 4318
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7085
DraI TTT'AAA
Cuts at: 2223 3258 3397 3972 4424 2223
Size: 1035 139 575 452 4919
Fragments arranged by size:
4919 1035 575 452 139
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6127 809
Size: 507 4811 1802
Fragments arranged by size:
4811 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2662 4567 5456
Size: 54 1425 50 275 18 832 1905 889
Cuts at: 5456 7085 8
Size: 1629 43
Fragments arranged by size:
1905 1629 1425 889 832 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4567 1537
Size: 275 2755 4090
Fragments arranged by size:
4090 2755 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2020 2166 2307 2541 2691 5050 1353
Size: 667 146 141 234 150 2359 3423
Fragments arranged by size:
3423 2359 667 234 150 146 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5707 6081 6227 387
Size: 1064 138 4118 374 146 1280
Fragments arranged by size:
4118 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 2273 2288 2304 2527 3055 3853
Size: 393 558 83 15 16 223 528 798
Cuts at: 3853 4119 5183 5780 6567 6835 1239
Size: 266 1064 597 787 268 1524
Fragments arranged by size:
1524 1064 798 787 597 558 528 393
268 266 223 83 16 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2734 3059 2734
Size: 325 6795
EcoO109I rG'GnC_Cy
Cuts at: 3052 3558 3614 3052
Size: 506 56 6558
Fragments arranged by size:
6558 506 56
EcoRI G'AATT_C
Cuts at: 3805 4429 4807 3805
Size: 624 378 6118
Fragments arranged by size:
6118 624 378
EcoRV GAT'ATC
Cuts at: 4084 4084
Size: 7120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3085 3605 3085
Size: 520 6600
FspI TGC'GCA
Cuts at: 5626 5626
Size: 7120
HaeII r_GCGC'y
Cuts at: 1678 6267 1678
Size: 4589 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 2502 2534 3106
Size: 32 135 32 623 32 275 32 572
Cuts at: 3106 3138 3491 3523 4288 4320 5761 5793 5835
Size: 32 353 32 765 32 1441 32 42
Cuts at: 5835 5867 1373
Size: 32 2626
Fragments arranged by size:
2626 1441 765 623 572 353 275 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4464 4710 669
Size: 988 281 2526 246 3079
Fragments arranged by size:
3079 2526 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2285 748
Size: 1537 5583
HpaI GTT'AAC
Cuts at: 4710 4710
Size: 7120
KpnI G_GTAC'C
Cuts at: 4445 4445
Size: 7120
MfeI C'AATT_G
Cuts at: 2778 4719 2778
Size: 1941 5179
MluI A'CGCG_T
Cuts at: 6996 6996
Size: 7120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6234 6418 1305
Size: 4929 184 2007
Fragments arranged by size:
4929 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5298
Fragments arranged by size:
5298 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2422 2724 2730 3092 3627 3860 3904
Size: 648 1256 302 6 362 535 233 44
Cuts at: 3904 4118 5078 5437 5596 518
Size: 214 960 359 159 2042
Fragments arranged by size:
2042 1256 960 648 535 362 359 302
233 214 159 44 6
MspA1I CmG'CkG
Cuts at: 1606 2293 2530 2661 4536 5213 6361 6606 1606
Size: 687 237 131 1875 677 1148 245 2120
Fragments arranged by size:
2120 1875 1148 687 677 245 237 131
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6568
NdeI CA'TA_TG
Cuts at: 387 3157 3841 3901 3949 4226 387
Size: 2770 684 60 48 277 3281
Fragments arranged by size:
3281 2770 684 277 60 48
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7120
NotI GC'GGCC_GC
Cuts at: 4567 4567
Size: 7120
NspI r_CATG'y
Cuts at: 1132 1229 3370 4478 6023 1132
Size: 97 2141 1108 1545 2229
Fragments arranged by size:
2229 2141 1545 1108 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7120
PciI A'CATG_T
Cuts at: 3366 6019 3366
Size: 2653 4467
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3048 4196 1108
Size: 1940 1148 4032
Fragments arranged by size:
4032 1940 1148
PfoI T'CCnGG_A
Cuts at: 4124 4381 4124
Size: 257 6863
PmeI GTTT'AAAC
Cuts at: 4424 4424
Size: 7120
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5189 5221 6702 6734 5189
Size: 32 1481 32 5575
Fragments arranged by size:
5575 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3052 3558 3052
Size: 506 6614
PshAI GACnn'nnGTC
Cuts at: 1613 3834 1613
Size: 2221 4899
PsiI TTA'TAA
Cuts at: 2154 4690 2154
Size: 2536 4584
PstI C_TGCA'G
Cuts at: 830 2298 3241 4472 830
Size: 1468 943 1231 3478
Fragments arranged by size:
3478 1468 1231 943
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7120
PvuII CAG'CTG
Cuts at: 2293 2530 2293
Size: 237 6883
SacII CC_GC'GG
Cuts at: 2662 2662
Size: 7120
SalI G'TCGA_C
Cuts at: 1936 4462 1936
Size: 2526 4594
SapI GCTCTTCn'nnn_
Cuts at: 2020 2307 2020
Size: 287 6833
SbfI CC_TGCA'GG
Cuts at: 4472 4472
Size: 7120
ScaI AGT'ACT
Cuts at: 2389 3965 5368 7080 2389
Size: 1576 1403 1712 2429
Fragments arranged by size:
2429 1712 1576 1403
SexAI A'CCwGG_T
Cuts at: 2440 2440
Size: 7120
SfcI C'TryA_G
Cuts at: 826 1045 2294 2383 2653 3237 3645 4468 5603
Size: 219 1249 89 270 584 408 823 1135
Cuts at: 5603 6284 6475 826
Size: 681 191 1471
Fragments arranged by size:
1471 1249 1135 823 681 584 408 270
219 191 89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7120
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7120
SmaI CCC'GGG
Cuts at: 1872 4447 1872
Size: 2575 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2976 5224 6125 6387 820
Size: 197 926 1033 2248 901 262 1553
Fragments arranged by size:
2248 1553 1033 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4911 493
Size: 4418 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7120
SphI G_CATG'C
Cuts at: 1132 4478 1132
Size: 3346 3774
SspI AAT'ATT
Cuts at: 5 52 2164 2925 5044 5
Size: 47 2112 761 2119 2081
Fragments arranged by size:
2119 2112 2081 761 47
StuI AGG'CCT
Cuts at: 1517 2906 1517
Size: 1389 5731
StyI C'CwwG_G
Cuts at: 513 1065 4559 513
Size: 552 3494 3074
Fragments arranged by size:
3494 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5147 5164 5317 5502 5947 325
Size: 1255 3567 17 153 185 445 1498
Fragments arranged by size:
3567 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2387 2838 3963
Size: 274 80 33 51 1443 410 451 1125
Cuts at: 3963 5366 7078 96
Size: 1403 1712 138
Fragments arranged by size:
1712 1443 1403 1125 451 410 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4248 4280 7080 7112 4248
Size: 32 2800 32 4256
Fragments arranged by size:
4256 2800 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7120
XbaI T'CTAG_A
Cuts at: 4456 4456
Size: 7120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2086 3212 2086
Size: 1126 5994
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7120
XmaI C'CCGG_G
Cuts at: 1870 4445 1870
Size: 2575 4545
XmnI GAAnn'nnTTC
Cuts at: 2721 3665 5249 2721
Size: 944 1584 4592
Fragments arranged by size:
4592 1584 944
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4928 276
Size: 53 83 186 936 3394 2468
Fragments arranged by size:
3394 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SanDI SfiI SfoI SrfI SwaI