Restriction map for
pFN21AE2094
(Circular) (Six-base) MAPSORT of: pFN21AE2094.seq Check: 6668 from: 1 to: 7093
pFN21AE2094 7093 bp
With 182 enzymes: SgfI *
March 19, 2012 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2196 2196
Size: 7093
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4903 278
Size: 53 83 186 936 3367 2468
Fragments arranged by size:
3367 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4272 4374 4436 1937
Size: 2335 102 62 4594
Fragments arranged by size:
4594 2335 102 62
Acc65I G'GTAC_C
Cuts at: 4188 4414 4188
Size: 226 6867
AclI AA'CG_TT
Cuts at: 1563 5220 5593 1563
Size: 3657 373 3063
Fragments arranged by size:
3657 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2612 3478 3662 5156 6540 6808 1632
Size: 980 866 184 1494 1384 268 1917
Fragments arranged by size:
1917 1494 1384 980 866 268 184
AfeI AGC'GCT
Cuts at: 2486 2486
Size: 7093
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6896
AflIII A'CryG_T
Cuts at: 1203 4131 5992 6969 1203
Size: 2928 1861 977 1327
Fragments arranged by size:
2928 1861 1327 977
AgeI A'CCGG_T
Cuts at: 5924 5924
Size: 7093
AhdI GACnn_n'nnGTC
Cuts at: 3992 5822 3992
Size: 1830 5263
AleI CACnn'nnGTG
Cuts at: 1166 2297 2882 1166
Size: 1131 585 5377
Fragments arranged by size:
5377 1131 585
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4085 4234 1184
Size: 2901 149 4043
Fragments arranged by size:
4043 2901 149
AlwNI CAG_nnn'CTG
Cuts at: 2239 2248 2431 2527 3886 3988 4836 6408 2239
Size: 9 183 96 1359 102 848 1572 2924
Fragments arranged by size:
2924 1572 1359 848 183 102 96 9
ApaI G_GGCC'C
Cuts at: 2170 2550 2170
Size: 380 6713
ApaLI G'TGCA_C
Cuts at: 3944 4334 5150 6306 3944
Size: 390 816 1156 4731
Fragments arranged by size:
4731 1156 816 390
ApoI r'AATT_y
Cuts at: 1493 4402 4628 4780 6889 1493
Size: 2909 226 152 2109 1697
Fragments arranged by size:
2909 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5647 160
Size: 872 4615 1606
Fragments arranged by size:
4615 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7093
AvaI C'yCGr_G
Cuts at: 1870 1943 2140 2157 3164 4091 4418 1870
Size: 73 197 17 1007 927 327 4545
Fragments arranged by size:
4545 1007 927 327 197 73 17
BamHI G'GATC_C
Cuts at: 2685 4423 4790 2685
Size: 1738 367 4988
Fragments arranged by size:
4988 1738 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2099 2323 2602 2646 2932
Size: 325 217 627 312 224 279 44 286
Cuts at: 2932 3560 4188 4363 4414 5869 618
Size: 628 628 175 51 1455 1842
Fragments arranged by size:
1842 1455 628 628 627 325 312 286
279 224 217 175 51 44
BanII G_rGCy'C
Cuts at: 1391 2170 2217 2256 2550 3462 4308 4412 1391
Size: 779 47 39 294 912 846 104 4072
Fragments arranged by size:
4072 912 846 779 294 104 47 39
BbeI G_GCGC'C
Cuts at: 2103 2327 3564 2103
Size: 224 1237 5632
Fragments arranged by size:
5632 1237 224
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3568 928
Size: 971 1669 4453
Fragments arranged by size:
4453 1669 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5284 5318 1903
Size: 34 50 34 3263 34 3678
Fragments arranged by size:
3678 3263 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4985 6195 4985
Size: 1210 5883
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7093
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2133 5704 136
Size: 107 122 71 631 605 461 3571 1525
Fragments arranged by size:
3571 1525 631 605 461 122 107 71
BglII A'GATC_T
Cuts at: 4161 7088 4161
Size: 2927 4166
BlpI GC'TnA_GC
Cuts at: 3687 4510 3687
Size: 823 6270
Bme1580I G_kGCm'C
Cuts at: 1790 2170 2470 2550 2586 2607 3948 4338 5154
Size: 380 300 80 36 21 1341 390 816
Cuts at: 5154 6310 1790
Size: 1156 2573
Fragments arranged by size:
2573 1341 1156 816 390 380 300 80
36 21
BmgBI CAC'GTC
Cuts at: 1318 2635 2796 3064 4132 4235 1318
Size: 1317 161 268 1068 103 4176
Fragments arranged by size:
4176 1317 1068 268 161 103
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2513 4824 5782
Size: 416 53 83 399 24 1096 2311 958
Cuts at: 5782 442
Size: 1753
Fragments arranged by size:
2311 1753 1096 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3900 1056
Size: 2844 4249
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 7093
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 2405 2690 2720 3023 3237 3360 3843
Size: 1031 135 285 30 303 214 123 483
Cuts at: 3843 5753 1239
Size: 1910 2579
Fragments arranged by size:
2579 1910 1031 483 303 285 214 135
123 30
Bpu10I CC'TnA_GC
Cuts at: 3606 3606
Size: 7093
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3524 5218 6083 6381 3524
Size: 1694 865 298 4236
Fragments arranged by size:
4236 1694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2315 2836 2969 3014 3213 3262 5756
Size: 617 816 521 133 45 199 49 2494
Cuts at: 5756 882
Size: 2219
Fragments arranged by size:
2494 2219 816 617 521 199 133 49
45
BsaAI yAC'GTr
Cuts at: 493 1204 4253 4884 493
Size: 711 3049 631 2702
Fragments arranged by size:
3049 2702 711 631
BsaBI GATnn'nnATC
Cuts at: 7087 7087
Size: 7093
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2100 2324 3561 3650
Size: 53 83 186 936 567 224 1237 89
Cuts at: 3650 3735 4900 5282 5917 275
Size: 85 1165 382 635 1451
Fragments arranged by size:
1451 1237 1165 936 635 567 382 224
186 89 85 83 53
BsaWI w'CCGG_w
Cuts at: 5526 5924 6198 6345 6756 5526
Size: 398 274 147 411 5863
Fragments arranged by size:
5863 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3210 3240 4181 4211 1178
Size: 30 165 30 1807 30 941 30 4060
Fragments arranged by size:
4060 1807 941 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2128 2337 2507 2510 1186
Size: 942 209 170 3 5769
Fragments arranged by size:
5769 942 209 170 3
BseYI C'CCAG_C
Cuts at: 2046 2487 2799 3550 4308 6296 6792 2046
Size: 441 312 751 758 1988 496 2347
Fragments arranged by size:
2347 1988 758 751 496 441 312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2429 2438 2858 2888 3724 1163
Size: 573 693 9 420 30 836 4532
Fragments arranged by size:
4532 836 693 573 420 30 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4543 5304 6332 1540
Size: 275 186 2542 761 1028 2301
Fragments arranged by size:
2542 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 2256 2970 3321 3540 3664 3948 4155 4338
Size: 39 714 351 219 124 284 207 183
Cuts at: 4338 5154 5239 6310 2217
Size: 816 85 1071 3000
Fragments arranged by size:
3000 1071 816 714 351 284 219 207
183 124 85 39
BsmI GAATG_Cn'
Cuts at: 4604 4697 4604
Size: 93 7000
BsmBI CGTCTCn'nnnn_
Cuts at: 2854 2943 2854
Size: 89 7004
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2170 2217 2256 2470 2550 2586 2607
Size: 399 380 47 39 214 80 36 21
Cuts at: 2607 2970 3321 3462 3540 3664 3948 4155 4308
Size: 363 351 141 78 124 284 207 153
Cuts at: 4308 4338 4412 5154 5239 6310 1391
Size: 30 74 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 380 363 351 284
214 207 153 141 124 85 80 78
74 47 39 36 30 21
BspEI T'CCGG_A
Cuts at: 6756 6756
Size: 7093
BspHI T'CATG_A
Cuts at: 3408 4982 6712 3408
Size: 1574 1730 3789
Fragments arranged by size:
3789 1730 1574
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2196 4448 844
Size: 1352 2252 3489
Fragments arranged by size:
3489 2252 1352
BsrBI CCG'CTC
Cuts at: 1394 1823 4246 4980 1394
Size: 429 2423 734 3507
Fragments arranged by size:
3507 2423 734 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5588 5762 65
Size: 1183 4340 174 1396
Fragments arranged by size:
4340 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2678 2929 3007 3193 5737 5924 1700
Size: 110 868 251 78 186 2544 187 2869
Fragments arranged by size:
2869 2544 868 251 187 186 110 78
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7093
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7093
BssSI C'ACGA_G
Cuts at: 5153 6165 5153
Size: 1012 6081
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2239 3721 3943 794
Size: 1445 1482 222 3944
Fragments arranged by size:
3944 1482 1445 222
BstBI TT'CG_AA
Cuts at: 5897 5897
Size: 7093
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7093
BstXI CCAn_nnnn'nTGG
Cuts at: 3835 3835
Size: 7093
BstYI r'GATC_y
Cuts at: 1972 2685 4161 4423 4790 5175 5192 6644 6730
Size: 713 1476 262 367 385 17 1452 86
Cuts at: 6730 6742 7088 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1476 1452 713 385 367 346 262
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2131 2712 3814 513
Size: 552 1066 581 1102 3792
Fragments arranged by size:
3792 1102 1066 581 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2164 487
Size: 683 56 938 5416
Fragments arranged by size:
5416 938 683 56
BtsI GCAGTG_nn'
Cuts at: 2537 4609 5403 5423 2537
Size: 2072 794 20 4207
Fragments arranged by size:
4207 2072 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7093
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3310 3345 529
Size: 35 2746 35 4277
Fragments arranged by size:
4277 2746 35 35
DraI TTT'AAA
Cuts at: 4397 4397
Size: 7093
DraIII CAC_nnn'GTG
Cuts at: 3721 3721
Size: 7093
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6100 809
Size: 507 4784 1802
Fragments arranged by size:
4784 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2757 2859 3106
Size: 54 1425 50 275 18 927 102 247
Cuts at: 3106 3276 3390 3957 4247 4540 5429 7058 8
Size: 170 114 567 290 293 889 1629 43
Fragments arranged by size:
1629 1425 927 889 567 293 290 275
247 170 114 102 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4540 1537
Size: 275 2728 4090
Fragments arranged by size:
4090 2728 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2297 2957 3299 5023 1353
Size: 944 660 342 1724 3423
Fragments arranged by size:
3423 1724 944 660 342
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2031 2523 3106 5680 6054 6200
Size: 1064 138 442 492 583 2574 374 146
Cuts at: 6200 387
Size: 1280
Fragments arranged by size:
2574 1280 1064 583 492 442 374 146
138
EcoICRI GAG'CTC
Cuts at: 2215 2254 2215
Size: 39 7054
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 2405 2612 2690 2720 3023 3237
Size: 393 638 135 207 78 30 303 214
Cuts at: 3237 3360 3478 3662 3843 5156 5753 6540 6808
Size: 123 118 184 181 1313 597 787 268
Cuts at: 6808 1239
Size: 1524
Fragments arranged by size:
1524 1313 787 638 597 393 303 268
214 207 184 181 135 123 118 78
30
EcoNI CCTnn'n_nnAGG
Cuts at: 2672 4358 2672
Size: 1686 5407
EcoO109I rG'GnC_Cy
Cuts at: 2166 2167 2269 2413 2546 2547 2665 3169 3790
Size: 1 102 144 133 1 118 504 621
Cuts at: 3790 3863 3952 2166
Size: 73 89 5307
Fragments arranged by size:
5307 621 504 144 133 118 102 89
73 1 1
EcoRI G'AATT_C
Cuts at: 4402 4780 4402
Size: 378 6715
EcoRV GAT'ATC
Cuts at: 4027 4027
Size: 7093
FspI TGC'GCA
Cuts at: 5599 5599
Size: 7093
HaeII r_GCGC'y
Cuts at: 1678 2103 2327 2488 3526 3564 6240 1678
Size: 425 224 161 1038 38 2676 2531
Fragments arranged by size:
2676 2531 1038 425 224 161 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3978 4010 5734 5766 5808
Size: 32 135 32 2406 32 1724 32 42
Cuts at: 5808 5840 1373
Size: 32 2626
Fragments arranged by size:
2626 2406 1724 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2845 3067 4437 4683 669
Size: 988 281 907 222 1370 246 3079
Fragments arranged by size:
3079 1370 988 907 281 246 222
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7093
HpaI GTT'AAC
Cuts at: 4683 4683
Size: 7093
KasI G'GCGC_C
Cuts at: 2099 2323 3560 2099
Size: 224 1237 5632
Fragments arranged by size:
5632 1237 224
KpnI G_GTAC'C
Cuts at: 4192 4418 4192
Size: 226 6867
MfeI C'AATT_G
Cuts at: 4692 4692
Size: 7093
MluI A'CGCG_T
Cuts at: 6969 6969
Size: 7093
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4221 4224 6207 6391 1305
Size: 2916 3 1983 184 2007
Fragments arranged by size:
2916 2007 1983 184 3
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2759 3392 3959 10
Size: 54 1425 343 927 633 567 3144
Fragments arranged by size:
3144 1425 927 633 567 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2297 2717 2882 5051 5410 5569 518
Size: 648 1131 420 165 2169 359 159 2042
Fragments arranged by size:
2169 2042 1131 648 420 359 165 159
MspA1I CmG'CkG
Cuts at: 1606 2046 2574 2872 3883 4300 4509 5186 6334
Size: 440 528 298 1011 417 209 677 1148
Cuts at: 6334 6579 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1011 677 528 440 417 298
245 209
NaeI GCC'GGC
Cuts at: 2680 2931 3009 2680
Size: 251 78 6764
Fragments arranged by size:
6764 251 78
NarI GG'CG_CC
Cuts at: 2100 2324 3561 2100
Size: 224 1237 5632
Fragments arranged by size:
5632 1237 224
NcoI C'CATG_G
Cuts at: 513 1065 3814 513
Size: 552 2749 3792
Fragments arranged by size:
3792 2749 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7093
NgoMIV G'CCGG_C
Cuts at: 2678 2929 3007 2678
Size: 251 78 6764
Fragments arranged by size:
6764 251 78
NheI G'CTAG_C
Cuts at: 1052 3896 1052
Size: 2844 4249
NotI GC'GGCC_GC
Cuts at: 4540 4540
Size: 7093
NspI r_CATG'y
Cuts at: 1132 1229 2563 4451 5996 1132
Size: 97 1334 1888 1545 2229
Fragments arranged by size:
2229 1888 1545 1334 97
PasI CC'CwG_GG
Cuts at: 1348 2743 3868 1348
Size: 1395 1125 4573
Fragments arranged by size:
4573 1395 1125
PciI A'CATG_T
Cuts at: 5992 5992
Size: 7093
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3383 3988 4080 1108
Size: 2275 605 92 4121
Fragments arranged by size:
4121 2275 605 92
PfoI T'CCnGG_A
Cuts at: 2396 3000 2396
Size: 604 6489
PmeI GTTT'AAAC
Cuts at: 4397 4397
Size: 7093
PmlI CAC'GTG
Cuts at: 4253 4253
Size: 7093
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5162 5194 6675 6707 5162
Size: 32 1481 32 5548
Fragments arranged by size:
5548 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3169 3790 3952 3169
Size: 621 162 6310
Fragments arranged by size:
6310 621 162
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7093
PsiI TTA'TAA
Cuts at: 4663 4663
Size: 7093
PspOMI G'GGCC_C
Cuts at: 2166 2546 2166
Size: 380 6713
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3473 3505 4097 4129 3473
Size: 32 592 32 6437
Fragments arranged by size:
6437 592 32 32
PstI C_TGCA'G
Cuts at: 830 2244 2532 3708 4445 830
Size: 1414 288 1176 737 3478
Fragments arranged by size:
3478 1414 1176 737 288
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7093
PvuII CAG'CTG
Cuts at: 2046 2574 2872 3883 2046
Size: 528 298 1011 5256
Fragments arranged by size:
5256 1011 528 298
SacI G_AGCT'C
Cuts at: 2217 2256 2217
Size: 39 7054
SalI G'TCGA_C
Cuts at: 1936 4435 1936
Size: 2499 4594
SapI GCTCTTCn'nnn_
Cuts at: 2957 2957
Size: 7093
SbfI CC_TGCA'GG
Cuts at: 4445 4445
Size: 7093
ScaI AGT'ACT
Cuts at: 5341 7053 5341
Size: 1712 5381
SfcI C'TryA_G
Cuts at: 826 1045 2240 2480 2528 3704 4030 4441 5576
Size: 219 1195 240 48 1176 326 411 1135
Cuts at: 5576 6257 6448 826
Size: 681 191 1471
Fragments arranged by size:
1471 1195 1176 1135 681 411 326 240
219 191 48
SfoI GGC'GCC
Cuts at: 2101 2325 3562 2101
Size: 224 1237 5632
Fragments arranged by size:
5632 1237 224
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7093
SgrAI Cr'CCGG_yG
Cuts at: 1700 3193 1700
Size: 1493 5600
SmaI CCC'GGG
Cuts at: 1872 2159 4420 1872
Size: 287 2261 4545
Fragments arranged by size:
4545 2261 287
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3539 5197 6098 6360 820
Size: 197 926 1596 1658 901 262 1553
Fragments arranged by size:
1658 1596 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4884 493
Size: 4391 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7093
SphI G_CATG'C
Cuts at: 1132 4451 1132
Size: 3319 3774
SspI AAT'ATT
Cuts at: 5 52 5017 5
Size: 47 4965 2081
Fragments arranged by size:
4965 2081 47
StuI AGG'CCT
Cuts at: 1517 3113 1517
Size: 1596 5497
StyI C'CwwG_G
Cuts at: 513 1065 3463 3814 4532 513
Size: 552 2398 351 718 3074
Fragments arranged by size:
3074 2398 718 552 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3074 3407 5120 5137 5290 5475 5920
Size: 1255 1494 333 1713 17 153 185 445
Cuts at: 5920 325
Size: 1498
Fragments arranged by size:
1713 1498 1494 1255 445 333 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5339 7051 96
Size: 274 80 33 51 1443 3362 1712 138
Fragments arranged by size:
3362 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3210 3242 7053 7085 3210
Size: 32 3811 32 3218
Fragments arranged by size:
3811 3218 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7093
XbaI T'CTAG_A
Cuts at: 4429 4429
Size: 7093
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2614 2614
Size: 7093
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7093
XmaI C'CCGG_G
Cuts at: 1870 2157 4418 1870
Size: 287 2261 4545
Fragments arranged by size:
4545 2261 287
XmnI GAAnn'nnTTC
Cuts at: 3575 5222 3575
Size: 1647 5446
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4901 276
Size: 53 83 186 936 3367 2468
Fragments arranged by size:
3367 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I FalI FseI FspAI NruI NsiI PacI PspXI
RsrII SacII SanDI SexAI SfiI SrfI SwaI