Restriction map for
pFN21AE2092
(Circular) (Six-base) MAPSORT of: pFN21AE2092.seq Check: 7562 from: 1 to: 7051
pFN21AE2092 7051 bp
With 182 enzymes: SgfI *
March 28, 2012 12:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3630 3630
Size: 7051
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3662 4861 278
Size: 53 83 186 936 2126 1199 2468
Fragments arranged by size:
2468 2126 1199 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3321 4394 1937
Size: 1384 1073 4594
Fragments arranged by size:
4594 1384 1073
Acc65I G'GTAC_C
Cuts at: 3771 4372 3771
Size: 601 6450
AclI AA'CG_TT
Cuts at: 1563 5178 5551 1563
Size: 3615 373 3063
Fragments arranged by size:
3615 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 3392 3758 3976 4295 4321 5114 6498
Size: 407 1353 366 218 319 26 793 1384
Cuts at: 6498 6766 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1353 793 407 366 319 268
218 26
AfeI AGC'GCT
Cuts at: 4025 4105 4025
Size: 80 6971
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6854
AflIII A'CryG_T
Cuts at: 1203 3825 5950 6927 1203
Size: 2622 2125 977 1327
Fragments arranged by size:
2622 2125 1327 977
AgeI A'CCGG_T
Cuts at: 5882 5882
Size: 7051
AhdI GACnn_n'nnGTC
Cuts at: 2791 3829 5780 2791
Size: 1038 1951 4062
Fragments arranged by size:
4062 1951 1038
AleI CACnn'nnGTG
Cuts at: 1166 3818 1166
Size: 2652 4399
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7051
AlwNI CAG_nnn'CTG
Cuts at: 2694 2873 3229 3286 3604 4794 6366 2694
Size: 179 356 57 318 1190 1572 3379
Fragments arranged by size:
3379 1572 1190 356 318 179 57
ApaI G_GGCC'C
Cuts at: 4331 4331
Size: 7051
ApaLI G'TGCA_C
Cuts at: 5108 6264 5108
Size: 1156 5895
ApoI r'AATT_y
Cuts at: 1493 2049 2113 4360 4586 4738 6847 1493
Size: 556 64 2247 226 152 2109 1697
Fragments arranged by size:
2247 2109 1697 556 226 152 64
AseI AT'TA_AT
Cuts at: 160 1032 5605 160
Size: 872 4573 1606
Fragments arranged by size:
4573 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7051
AvaI C'yCGr_G
Cuts at: 1870 1943 3881 4088 4222 4376 1870
Size: 73 1938 207 134 154 4545
Fragments arranged by size:
4545 1938 207 154 134 73
BamHI G'GATC_C
Cuts at: 3636 3728 4381 4748 3636
Size: 92 653 367 5939
Fragments arranged by size:
5939 653 367 92
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3305 3771 3797 4214 4372
Size: 325 217 627 1518 466 26 417 158
Cuts at: 4372 5827 618
Size: 1455 1842
Fragments arranged by size:
1842 1518 1455 627 466 417 325 217
158 26
BanII G_rGCy'C
Cuts at: 1391 3219 3969 4036 4331 4370 1391
Size: 1828 750 67 295 39 4072
Fragments arranged by size:
4072 1828 750 295 67 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2220 2232 2834 3590 928
Size: 971 321 12 602 756 4389
Fragments arranged by size:
4389 971 756 602 321 12
BbvCI CC'TCA_GC
Cuts at: 3413 4107 3413
Size: 694 6357
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2261 2295 2384 2418 3179 3213 4009
Size: 34 324 34 89 34 761 34 796
Cuts at: 4009 4043 5242 5276 1903
Size: 34 1199 34 3678
Fragments arranged by size:
3678 1199 796 761 324 89 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4943 6153 4943
Size: 1210 5841
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7051
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3684 5662 136
Size: 107 122 71 631 605 2012 1978 1525
Fragments arranged by size:
2012 1978 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3498 7046 3498
Size: 3548 3503
BlpI GC'TnA_GC
Cuts at: 3674 4468 3674
Size: 794 6257
Bme1580I G_kGCm'C
Cuts at: 1790 3189 3308 4217 4331 5112 6268 1790
Size: 1399 119 909 114 781 1156 2573
Fragments arranged by size:
2573 1399 1156 909 781 119 114
BmgBI CAC'GTC
Cuts at: 1318 3408 1318
Size: 2090 4961
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4782 5740 442
Size: 416 53 83 399 24 3365 958 1753
Fragments arranged by size:
3365 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7051
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2716 5711 1239
Size: 1477 2995 2579
Fragments arranged by size:
2995 2579 1477
Bpu10I CC'TnA_GC
Cuts at: 2866 3413 4107 2866
Size: 547 694 5810
Fragments arranged by size:
5810 694 547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2168 3203 3419 3518 3638 3677 4103 4328 5176
Size: 1035 216 99 120 39 426 225 848
Cuts at: 5176 6041 6339 2168
Size: 865 298 2880
Fragments arranged by size:
2880 1035 865 848 426 298 225 216
120 99 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2966 3542 4133 5714 882
Size: 617 1467 576 591 1581 2219
Fragments arranged by size:
2219 1581 1467 617 591 576
BsaAI yAC'GTr
Cuts at: 493 1204 4176 4842 493
Size: 711 2972 666 2702
Fragments arranged by size:
2972 2702 711 666
BsaBI GATnn'nnATC
Cuts at: 3503 7045 3503
Size: 3542 3509
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3659 4858 5240 5875
Size: 53 83 186 936 2126 1199 382 635
Cuts at: 5875 275
Size: 1451
Fragments arranged by size:
2126 1451 1199 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5484 5882 6156 6303 6714 5484
Size: 398 274 147 411 5821
Fragments arranged by size:
5821 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2908 2938 3266 3296 1178
Size: 30 165 30 1505 30 328 30 4933
Fragments arranged by size:
4933 1505 328 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2165 2247 2615 2900 2903 2906 2915
Size: 913 66 82 368 285 3 3 9
Cuts at: 2915 2918 2921 2951 2954 2957 2960 2975 2978
Size: 3 3 30 3 3 3 15 3
Cuts at: 2978 2981 2984 3005 3287 3314 4108 1186
Size: 3 3 21 282 27 794 4129
Fragments arranged by size:
4129 913 794 368 285 282 82 66
30 27 21 15 9 3 3 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2268 3441 3970 4258 6254 6750 2268
Size: 1173 529 288 1996 496 2569
Fragments arranged by size:
2569 1996 1173 529 496 288
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2625 3608 3626 1163
Size: 573 889 983 18 4588
Fragments arranged by size:
4588 983 889 573 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2717 2749 2841 3100 3756 3819
Size: 275 186 716 32 92 259 656 63
Cuts at: 3819 4501 5262 6290 1540
Size: 682 761 1028 2301
Fragments arranged by size:
2301 1028 761 716 682 656 275 259
186 92 63 32
BsiHKAI G_wGCw'C
Cuts at: 2397 2921 3219 3528 3969 4002 5112 5197 6268
Size: 524 298 309 441 33 1110 85 1071
Cuts at: 6268 2397
Size: 3180
Fragments arranged by size:
3180 1110 1071 524 441 309 298 85
33
BsmI GAATG_Cn'
Cuts at: 2482 4562 4655 2482
Size: 2080 93 4878
Fragments arranged by size:
4878 2080 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2736 4274 2736
Size: 1538 5513
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2397 2921 3189 3219 3308 3528 3969
Size: 399 607 524 268 30 89 220 441
Cuts at: 3969 4002 4036 4217 4331 4370 5112 5197 6268
Size: 33 34 181 114 39 742 85 1071
Cuts at: 6268 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 607 524 441 399 268
220 181 114 89 85 39 34 33
30
BspEI T'CCGG_A
Cuts at: 6714 6714
Size: 7051
BspHI T'CATG_A
Cuts at: 4062 4940 6670 4062
Size: 878 1730 4443
Fragments arranged by size:
4443 1730 878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2796 3249 3630 3675 4406 844
Size: 1952 453 381 45 731 3489
Fragments arranged by size:
3489 1952 731 453 381 45
BsrBI CCG'CTC
Cuts at: 1394 1823 2533 2590 2605 2617 2728 4938 1394
Size: 429 710 57 15 12 111 2210 3507
Fragments arranged by size:
3507 2210 710 429 111 57 15 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 5546 5720 65
Size: 1183 4298 174 1396
Fragments arranged by size:
4298 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2975 3244 3683 5695 5882 1700
Size: 110 1165 269 439 2012 187 2869
Fragments arranged by size:
2869 2012 1165 439 269 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7051
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7051
BssSI C'ACGA_G
Cuts at: 5111 6123 5111
Size: 1012 6039
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3229 794
Size: 2435 4616
BstBI TT'CG_AA
Cuts at: 5855 5855
Size: 7051
BstEII G'GTnAC_C
Cuts at: 1184 3760 1184
Size: 2576 4475
BstXI CCAn_nnnn'nTGG
Cuts at: 3820 3820
Size: 7051
BstYI r'GATC_y
Cuts at: 1972 3498 3636 3728 3922 4381 4748 5133 5150
Size: 1526 138 92 194 459 367 385 17
Cuts at: 5150 6602 6688 6700 7046 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1526 1452 459 385 367 346 194
138 92 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4233 4233
Size: 7051
BtgI C'CryG_G
Cuts at: 513 1065 2004 2720 3813 3819 4323 513
Size: 552 939 716 1093 6 504 3241
Fragments arranged by size:
3241 1093 939 716 552 504 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6312
Fragments arranged by size:
6312 683 56
BtsI GCAGTG_nn'
Cuts at: 4567 5361 5381 4567
Size: 794 20 6237
Fragments arranged by size:
6237 794 20
ClaI AT'CG_AT
Cuts at: 1554 3897 1554
Size: 2343 4708
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7016
DraI TTT'AAA
Cuts at: 4355 4355
Size: 7051
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6058 809
Size: 507 4742 1802
Fragments arranged by size:
4742 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 2714 2746
Size: 54 1425 50 275 18 423 461 32
Cuts at: 2746 2838 3816 4498 5387 7016 8
Size: 92 978 682 889 1629 43
Fragments arranged by size:
1629 1425 978 889 682 461 423 275
92 54 50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 2714 2746 2838 3816 4498 1537
Size: 275 902 32 92 978 682 4090
Fragments arranged by size:
4090 978 902 682 275 92 32
EarI CTCTTCn'nnn_
Cuts at: 1353 2203 2453 2760 2877 2892 2946 2952 4981
Size: 850 250 307 117 15 54 6 2029
Cuts at: 4981 1353
Size: 3423
Fragments arranged by size:
3423 2029 850 307 250 117 54 15
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2612 5638 6012 6158 387
Size: 1064 138 1023 3026 374 146 1280
Fragments arranged by size:
3026 1280 1064 1023 374 146 138
EcoICRI GAG'CTC
Cuts at: 3217 3967 3217
Size: 750 6301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2716 3392 3758 3976 4295 4321
Size: 393 407 677 676 366 218 319 26
Cuts at: 4321 5114 5711 6498 6766 1239
Size: 793 597 787 268 1524
Fragments arranged by size:
1524 793 787 677 676 597 407 393
366 319 268 218 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2150 3170 4236 2150
Size: 1020 1066 4965
Fragments arranged by size:
4965 1066 1020
EcoO109I rG'GnC_Cy
Cuts at: 2785 3930 4227 4314 4328 2785
Size: 1145 297 87 14 5508
Fragments arranged by size:
5508 1145 297 87 14
EcoRI G'AATT_C
Cuts at: 4360 4738 4360
Size: 378 6673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3412 4006 3412
Size: 594 6457
FspI TGC'GCA
Cuts at: 5557 5557
Size: 7051
HaeII r_GCGC'y
Cuts at: 1678 3165 4027 4107 6198 1678
Size: 1487 862 80 2091 2531
Fragments arranged by size:
2531 2091 1487 862 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2777 2809 2834 2866 2981
Size: 32 135 32 1205 32 25 32 115
Cuts at: 2981 3013 3713 3745 3815 3847 5692 5724 5766
Size: 32 700 32 70 32 1845 32 42
Cuts at: 5766 5798 1373
Size: 32 2626
Fragments arranged by size:
2626 1845 1205 700 135 115 70 42
32 32 32 32 32 32 32 32
32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 4395 4641 669
Size: 988 281 2457 246 3079
Fragments arranged by size:
3079 2457 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7051
HpaI GTT'AAC
Cuts at: 4641 4641
Size: 7051
KpnI G_GTAC'C
Cuts at: 3775 4376 3775
Size: 601 6450
MfeI C'AATT_G
Cuts at: 4650 4650
Size: 7051
MluI A'CGCG_T
Cuts at: 6927 6927
Size: 7051
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2384 2820 3687 6165 6349 1305
Size: 1079 436 867 2478 184 2007
Fragments arranged by size:
2478 2007 1079 867 436 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2255 10
Size: 54 1425 343 423 4806
Fragments arranged by size:
4806 1425 423 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3509 3818 5009 5368 5527 518
Size: 648 2343 309 1191 359 159 2042
Fragments arranged by size:
2343 2042 1191 648 359 309 159
MspA1I CmG'CkG
Cuts at: 1606 2569 2722 2855 3601 4258 4325 4467 5144
Size: 963 153 133 746 657 67 142 677
Cuts at: 5144 6292 6537 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 963 746 677 657 245 153
142 133 67
NaeI GCC'GGC
Cuts at: 3246 3685 3246
Size: 439 6612
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5560
Fragments arranged by size:
5560 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7051
NgoMIV G'CCGG_C
Cuts at: 3244 3683 3244
Size: 439 6612
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7051
NotI GC'GGCC_GC
Cuts at: 4498 4498
Size: 7051
NspI r_CATG'y
Cuts at: 1132 1229 2369 3829 4409 5954 1132
Size: 97 1140 1460 580 1545 2229
Fragments arranged by size:
2229 1545 1460 1140 580 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7051
PciI A'CATG_T
Cuts at: 3825 5950 3825
Size: 2125 4926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2694 3073 1108
Size: 1586 379 5086
Fragments arranged by size:
5086 1586 379
PfoI T'CCnGG_A
Cuts at: 2486 2486
Size: 7051
PmeI GTTT'AAAC
Cuts at: 4355 4355
Size: 7051
PmlI CAC'GTG
Cuts at: 4176 4176
Size: 7051
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3077 3109 5120 5152 6633 6665 3077
Size: 32 2011 32 1481 32 3463
Fragments arranged by size:
3463 2011 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2785 3930 2785
Size: 1145 5906
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7051
PsiI TTA'TAA
Cuts at: 4621 4621
Size: 7051
PspOMI G'GGCC_C
Cuts at: 4327 4327
Size: 7051
PstI C_TGCA'G
Cuts at: 830 3255 4403 830
Size: 2425 1148 3478
Fragments arranged by size:
3478 2425 1148
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7051
PvuII CAG'CTG
Cuts at: 3601 4258 3601
Size: 657 6394
SacI G_AGCT'C
Cuts at: 3219 3969 3219
Size: 750 6301
SacII CC_GC'GG
Cuts at: 2723 4326 2723
Size: 1603 5448
SalI G'TCGA_C
Cuts at: 1936 4393 1936
Size: 2457 4594
SanDI GG'GwC_CC
Cuts at: 3930 3930
Size: 7051
SbfI CC_TGCA'GG
Cuts at: 3255 4403 3255
Size: 1148 5903
ScaI AGT'ACT
Cuts at: 5299 7011 5299
Size: 1712 5339
SfcI C'TryA_G
Cuts at: 826 1045 2668 3251 3322 3958 4249 4399 5534
Size: 219 1623 583 71 636 291 150 1135
Cuts at: 5534 6215 6406 826
Size: 681 191 1471
Fragments arranged by size:
1623 1471 1135 681 636 583 291 219
191 150 71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7051
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7051
SmaI CCC'GGG
Cuts at: 1872 3883 4090 4378 1872
Size: 2011 207 288 4545
Fragments arranged by size:
4545 2011 288 207
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2147 3218 3434 3533 3653 3692
Size: 197 926 204 1071 216 99 120 39
Cuts at: 3692 4118 4343 5155 6056 6318 820
Size: 426 225 812 901 262 1553
Fragments arranged by size:
1553 1071 926 901 812 426 262 225
216 204 197 120 99 39
SnaBI TAC'GTA
Cuts at: 493 4842 493
Size: 4349 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7051
SphI G_CATG'C
Cuts at: 1132 2369 4409 1132
Size: 1237 2040 3774
Fragments arranged by size:
3774 2040 1237
SspI AAT'ATT
Cuts at: 5 52 4975 5
Size: 47 4923 2081
Fragments arranged by size:
4923 2081 47
StuI AGG'CCT
Cuts at: 1517 3743 4242 1517
Size: 2226 499 4326
Fragments arranged by size:
4326 2226 499
StyI C'CwwG_G
Cuts at: 513 1065 2004 3801 4490 513
Size: 552 939 1797 689 3074
Fragments arranged by size:
3074 1797 939 689 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 3114 3128 3689 4250 5078 5095
Size: 1255 417 1117 14 561 561 828 17
Cuts at: 5095 5248 5433 5878 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1117 828 561 561 445 417
185 153 17 14
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5297 7009 96
Size: 274 80 33 51 1443 3320 1712 138
Fragments arranged by size:
3320 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2908 2940 3266 3298 3922 3954 7011 7043 2908
Size: 32 326 32 624 32 3057 32 2916
Fragments arranged by size:
3057 2916 624 326 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7051
XbaI T'CTAG_A
Cuts at: 4387 4387
Size: 7051
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3605 3605
Size: 7051
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7051
XmaI C'CCGG_G
Cuts at: 1870 3881 4088 4376 1870
Size: 2011 207 288 4545
Fragments arranged by size:
4545 2011 288 207
XmnI GAAnn'nnTTC
Cuts at: 2052 4070 5180 2052
Size: 2018 1110 3923
Fragments arranged by size:
3923 2018 1110
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3660 4859 276
Size: 53 83 186 936 2126 1199 2468
Fragments arranged by size:
2468 2126 1199 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PspXI PsrI RsrII SapI SexAI SfiI
SfoI SrfI SwaI