Restriction map for
pFN21AE2091
(Circular) (Six-base) MAPSORT of: pFN21AE2091.seq Check: 7696 from: 1 to: 7000
pFN21AE2091 7000 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2466 2466
Size: 7000
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4810 278
Size: 53 83 186 936 3274 2468
Fragments arranged by size:
3274 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3027 4343 1937
Size: 1090 1316 4594
Fragments arranged by size:
4594 1316 1090
Acc65I G'GTAC_C
Cuts at: 4321 4321
Size: 7000
AclI AA'CG_TT
Cuts at: 1563 5127 5500 1563
Size: 3564 373 3063
Fragments arranged by size:
3564 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2789 3469 3992 4160 5063 6447 6715 1632
Size: 1157 680 523 168 903 1384 268 1917
Fragments arranged by size:
1917 1384 1157 903 680 523 268 168
AfeI AGC'GCT
Cuts at: 2261 3193 2261
Size: 932 6068
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6803
AflIII A'CryG_T
Cuts at: 1203 3417 5899 6876 1203
Size: 2214 2482 977 1327
Fragments arranged by size:
2482 2214 1327 977
AgeI A'CCGG_T
Cuts at: 5831 5831
Size: 7000
AhdI GACnn_n'nnGTC
Cuts at: 5729 5729
Size: 7000
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7000
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3726 1184
Size: 2542 4458
AlwNI CAG_nnn'CTG
Cuts at: 2818 2830 3511 4186 4743 6315 2818
Size: 12 681 675 557 1572 3503
Fragments arranged by size:
3503 1572 681 675 557 12
ApaI G_GGCC'C
Cuts at: 2805 2871 3619 4071 4267 2805
Size: 66 748 452 196 5538
Fragments arranged by size:
5538 748 452 196 66
ApaLI G'TGCA_C
Cuts at: 3563 5057 6213 3563
Size: 1494 1156 4350
Fragments arranged by size:
4350 1494 1156
ApoI r'AATT_y
Cuts at: 1493 3005 4309 4535 4687 6796 1493
Size: 1512 1304 226 152 2109 1697
Fragments arranged by size:
2109 1697 1512 1304 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5554 160
Size: 872 4522 1606
Fragments arranged by size:
4522 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
AvaI C'yCGr_G
Cuts at: 1870 1943 3611 3668 3716 3977 4055 4081 4234
Size: 73 1668 57 48 261 78 26 153
Cuts at: 4234 4325 1870
Size: 91 4545
Fragments arranged by size:
4545 1668 261 153 91 78 73 57
48 26
BamHI G'GATC_C
Cuts at: 2763 2842 4330 4697 2763
Size: 79 1488 367 5066
Fragments arranged by size:
5066 1488 367 79
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2287 2600 3091 3673 4321
Size: 325 217 627 500 313 491 582 648
Cuts at: 4321 5776 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 648 627 582 500 491 325
313 217
BanII G_rGCy'C
Cuts at: 1391 2805 2871 3619 3945 4071 4217 4246 4267
Size: 1414 66 748 326 126 146 29 21
Cuts at: 4267 4319 1391
Size: 52 4072
Fragments arranged by size:
4072 1414 748 326 146 126 66 52
29 21
BbeI G_GCGC'C
Cuts at: 2291 2291
Size: 7000
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6029
BbvCI CC'TCA_GC
Cuts at: 2412 2748 2412
Size: 336 6664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3329 3363 4062 4096 5191 5225 1903
Size: 34 1392 34 699 34 1095 34 3678
Fragments arranged by size:
3678 1392 1095 699 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3071 3922 4892 6102 3071
Size: 851 970 1210 3969
Fragments arranged by size:
3969 1210 970 851
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7000
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3282 5611 136
Size: 107 122 71 631 605 1610 2329 1525
Fragments arranged by size:
2329 1610 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6995 6995
Size: 7000
BlpI GC'TnA_GC
Cuts at: 2549 4417 2549
Size: 1868 5132
Bme1580I G_kGCm'C
Cuts at: 1790 2076 2126 2172 2805 2871 2881 3567 3619
Size: 286 50 46 633 66 10 686 52
Cuts at: 3619 3678 4071 4267 5061 6217 1790
Size: 59 393 196 794 1156 2573
Fragments arranged by size:
2573 1156 794 686 633 393 286 196
66 59 52 50 46 10
BmgBI CAC'GTC
Cuts at: 1318 4051 1318
Size: 2733 4267
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4731 5689 442
Size: 416 53 83 399 24 3314 958 1753
Fragments arranged by size:
3314 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7000
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 2516 2744 3038 3177 3341 3452 3479
Size: 1052 225 228 294 139 164 111 27
Cuts at: 3479 3781 5660 1239
Size: 302 1879 2579
Fragments arranged by size:
2579 1879 1052 302 294 228 225 164
139 111 27
Bpu10I CC'TnA_GC
Cuts at: 2412 2748 2412
Size: 336 6664
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2096 3560 5125 5990 6288 2096
Size: 1464 1565 865 298 2808
Fragments arranged by size:
2808 1565 1464 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2478 3153 4091 5663 882
Size: 617 979 675 938 1572 2219
Fragments arranged by size:
2219 1572 979 938 675 617
BsaAI yAC'GTr
Cuts at: 493 1204 3420 4791 493
Size: 711 2216 1371 2702
Fragments arranged by size:
2702 2216 1371 711
BsaBI GATnn'nnATC
Cuts at: 6994 6994
Size: 7000
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2288 3686 3771 4043
Size: 53 83 186 936 755 1398 85 272
Cuts at: 4043 4807 5189 5824 275
Size: 764 382 635 1451
Fragments arranged by size:
1451 1398 936 764 755 635 382 272
186 85 83 53
BsaWI w'CCGG_w
Cuts at: 2889 4038 5433 5831 6105 6252 6663 2889
Size: 1149 1395 398 274 147 411 3226
Fragments arranged by size:
3226 1395 1149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6775
Fragments arranged by size:
6775 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2585 3563 4015 1186
Size: 907 492 978 452 4171
Fragments arranged by size:
4171 978 907 492 452
BseYI C'CCAG_C
Cuts at: 2119 3232 6203 6699 2119
Size: 1113 2971 496 2420
Fragments arranged by size:
2971 2420 1113 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2243 2291 2321 2444 3374 3398 4206
Size: 573 507 48 30 123 930 24 808
Cuts at: 4206 1163
Size: 3957
Fragments arranged by size:
3957 930 808 573 507 123 48 30
24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3531 3659 3849 4450 5211 6239
Size: 275 186 1530 128 190 601 761 1028
Cuts at: 6239 1540
Size: 2301
Fragments arranged by size:
2301 1530 1028 761 601 275 190 186
128
BsiHKAI G_wGCw'C
Cuts at: 2247 2777 3465 3567 4056 5061 5146 6217 2247
Size: 530 688 102 489 1005 85 1071 3030
Fragments arranged by size:
3030 1071 1005 688 530 489 102 85
BsmI GAATG_Cn'
Cuts at: 4106 4159 4511 4604 4106
Size: 53 352 93 6502
Fragments arranged by size:
6502 352 93 53
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2126 2172 2247 2777 2805 2871
Size: 399 286 50 46 75 530 28 66
Cuts at: 2871 2881 3465 3567 3619 3678 3945 4056 4071
Size: 10 584 102 52 59 267 111 15
Cuts at: 4071 4217 4246 4267 4319 5061 5146 6217 1391
Size: 146 29 21 52 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 584 530 399 286 267
146 111 102 85 75 66 59 52
52 50 46 29 28 21 15 10
BspEI T'CCGG_A
Cuts at: 2889 4038 6663 2889
Size: 1149 2625 3226
Fragments arranged by size:
3226 2625 1149
BspHI T'CATG_A
Cuts at: 4889 6619 4889
Size: 1730 5270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2466 2826 3225 3790 4355 844
Size: 1622 360 399 565 565 3489
Fragments arranged by size:
3489 1622 565 565 399 360
BsrBI CCG'CTC
Cuts at: 1394 1823 2361 2581 2973 3282 4036 4887 1394
Size: 429 538 220 392 309 754 851 3507
Fragments arranged by size:
3507 851 754 538 429 392 309 220
BsrDI GCAATG_nn'
Cuts at: 65 1248 5495 5669 65
Size: 1183 4247 174 1396
Fragments arranged by size:
4247 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3162 5644 5831 1700
Size: 110 1352 2482 187 2869
Fragments arranged by size:
2869 2482 1352 187 110
BsrGI T'GTAC_A
Cuts at: 96 3780 96
Size: 3684 3316
BssHII G'CGCG_C
Cuts at: 1925 2227 4017 4019 1925
Size: 302 1790 2 4906
Fragments arranged by size:
4906 1790 302 2
BssSI C'ACGA_G
Cuts at: 2075 3392 3521 3545 3997 5060 6072 2075
Size: 1317 129 24 452 1063 1012 3003
Fragments arranged by size:
3003 1317 1063 1012 452 129 24
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2818 2830 3397 4186 794
Size: 2024 12 567 789 3608
Fragments arranged by size:
3608 2024 789 567 12
BstBI TT'CG_AA
Cuts at: 5804 5804
Size: 7000
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7000
BstXI CCAn_nnnn'nTGG
Cuts at: 3931 4102 3931
Size: 171 6829
BstYI r'GATC_y
Cuts at: 1972 2763 2842 3303 3507 4330 4697 5082 5099
Size: 791 79 461 204 823 367 385 17
Cuts at: 5099 6551 6637 6649 6995 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 823 791 461 385 367 346
204 86 79 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2037 2805 3951 513
Size: 552 939 33 768 1146 3562
Fragments arranged by size:
3562 1146 939 768 552 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6261
Fragments arranged by size:
6261 683 56
BtsI GCAGTG_nn'
Cuts at: 4080 4516 5310 5330 4080
Size: 436 794 20 5750
Fragments arranged by size:
5750 794 436 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7000
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6965
DraI TTT'AAA
Cuts at: 4304 4304
Size: 7000
DraIII CAC_nnn'GTG
Cuts at: 3397 3397
Size: 7000
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6007 809
Size: 507 4691 1802
Fragments arranged by size:
4691 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3164 3183 3283
Size: 54 1425 50 275 18 1334 19 100
Cuts at: 3283 3528 3656 3823 3846 4164 4447 5336 6965
Size: 245 128 167 23 318 283 889 1629
Cuts at: 6965 8
Size: 43
Fragments arranged by size:
1629 1425 1334 889 318 283 275 245
167 128 100 54 50 43 23 19
18
EagI C'GGCC_G
Cuts at: 1537 1812 3528 3656 3846 4447 1537
Size: 275 1716 128 190 601 4090
Fragments arranged by size:
4090 1716 601 275 190 128
EarI CTCTTCn'nnn_
Cuts at: 1353 2693 4136 4930 1353
Size: 1340 1443 794 3423
Fragments arranged by size:
3423 1443 1340 794
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4189 5587 5961 6107 387
Size: 1064 138 2600 1398 374 146 1280
Fragments arranged by size:
2600 1398 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2516 2744 2789 3038 3177 3341
Size: 393 659 225 228 45 249 139 164
Cuts at: 3341 3452 3469 3479 3781 3992 4160 5063 5660
Size: 111 17 10 302 211 168 903 597
Cuts at: 5660 6447 6715 1239
Size: 787 268 1524
Fragments arranged by size:
1524 903 787 659 597 393 302 268
249 228 225 211 168 164 139 111
45 17 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2567 2567
Size: 7000
EcoO109I rG'GnC_Cy
Cuts at: 2706 2815 2867 2868 2911 3058 3615 3616 3802
Size: 109 52 1 43 147 557 1 186
Cuts at: 3802 3816 4263 4264 2706
Size: 14 447 1 5442
Fragments arranged by size:
5442 557 447 186 147 109 52 43
14 1 1 1
EcoRI G'AATT_C
Cuts at: 3005 4309 4687 3005
Size: 1304 378 5318
Fragments arranged by size:
5318 1304 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3499 3499
Size: 7000
FspI TGC'GCA
Cuts at: 2207 3515 5506 2207
Size: 1308 1991 3701
Fragments arranged by size:
3701 1991 1308
HaeII r_GCGC'y
Cuts at: 1678 2219 2263 2291 2395 3149 3195 3277 3880
Size: 541 44 28 104 754 46 82 603
Cuts at: 3880 4197 6147 1678
Size: 317 1950 2531
Fragments arranged by size:
2531 1950 754 603 541 317 104 82
46 44 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3295 3327 4233 4265 5641
Size: 32 135 32 1723 32 906 32 1376
Cuts at: 5641 5673 5715 5747 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1723 1376 906 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4344 4590 669
Size: 988 281 2406 246 3079
Fragments arranged by size:
3079 2406 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7000
HpaI GTT'AAC
Cuts at: 4590 4590
Size: 7000
KasI G'GCGC_C
Cuts at: 2287 2287
Size: 7000
KpnI G_GTAC'C
Cuts at: 4325 4325
Size: 7000
MfeI C'AATT_G
Cuts at: 4599 4599
Size: 7000
MluI A'CGCG_T
Cuts at: 6876 6876
Size: 7000
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3474 6114 6298 1305
Size: 2169 2640 184 2007
Fragments arranged by size:
2640 2169 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3185 3825 4166 10
Size: 54 1425 343 1353 640 341 2844
Fragments arranged by size:
2844 1425 1353 640 343 341 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2198 4100 4958 5317 5476 518
Size: 648 1032 1902 858 359 159 2042
Fragments arranged by size:
2042 1902 1032 858 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2827 3179 3264 3412 4416 5093 6241 6486
Size: 1221 352 85 148 1004 677 1148 245
Cuts at: 6486 1606
Size: 2120
Fragments arranged by size:
2120 1221 1148 1004 677 352 245 148
85
NarI GG'CG_CC
Cuts at: 2288 2288
Size: 7000
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5509
Fragments arranged by size:
5509 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7000
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7000
NotI GC'GGCC_GC
Cuts at: 3656 4447 3656
Size: 791 6209
NspI r_CATG'y
Cuts at: 1132 1229 4358 5903 1132
Size: 97 3129 1545 2229
Fragments arranged by size:
3129 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2872 1348
Size: 1524 5476
PciI A'CATG_T
Cuts at: 5899 5899
Size: 7000
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7000
PfoI T'CCnGG_A
Cuts at: 2346 3429 2346
Size: 1083 5917
PmeI GTTT'AAAC
Cuts at: 4304 4304
Size: 7000
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5069 5101 6582 6614 5069
Size: 32 1481 32 5455
Fragments arranged by size:
5455 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2815 3058 3802 2815
Size: 243 744 6013
Fragments arranged by size:
6013 744 243
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7000
PsiI TTA'TAA
Cuts at: 4570 4570
Size: 7000
PspOMI G'GGCC_C
Cuts at: 2801 2867 3615 4067 4263 2801
Size: 66 748 452 196 5538
Fragments arranged by size:
5538 748 452 196 66
PspXI vC'TCGA_Gb
Cuts at: 3977 4055 3977
Size: 78 6922
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3578 3610 3578
Size: 32 6968
PstI C_TGCA'G
Cuts at: 830 2826 3705 4008 4352 830
Size: 1996 879 303 344 3478
Fragments arranged by size:
3478 1996 879 344 303
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7000
PvuII CAG'CTG
Cuts at: 3412 3412
Size: 7000
SalI G'TCGA_C
Cuts at: 1936 4342 1936
Size: 2406 4594
SanDI GG'GwC_CC
Cuts at: 3058 3058
Size: 7000
SapI GCTCTTCn'nnn_
Cuts at: 2693 4136 2693
Size: 1443 5557
SbfI CC_TGCA'GG
Cuts at: 4352 4352
Size: 7000
ScaI AGT'ACT
Cuts at: 2399 5248 6960 2399
Size: 2849 1712 2439
Fragments arranged by size:
2849 2439 1712
SexAI A'CCwGG_T
Cuts at: 3423 3423
Size: 7000
SfcI C'TryA_G
Cuts at: 826 1045 2822 3701 4004 4348 5483 6164 6355
Size: 219 1777 879 303 344 1135 681 191
Cuts at: 6355 826
Size: 1471
Fragments arranged by size:
1777 1471 1135 879 681 344 303 219
191
SfoI GGC'GCC
Cuts at: 2289 2289
Size: 7000
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7000
SmaI CCC'GGG
Cuts at: 1872 3613 3670 4236 4327 1872
Size: 1741 57 566 91 4545
Fragments arranged by size:
4545 1741 566 91 57
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2111 3575 3977 4055 5104 6005
Size: 197 926 168 1464 402 78 1049 901
Cuts at: 6005 6267 820
Size: 262 1553
Fragments arranged by size:
1553 1464 1049 926 901 402 262 197
168 78
SnaBI TAC'GTA
Cuts at: 493 4791 493
Size: 4298 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7000
SphI G_CATG'C
Cuts at: 1132 4358 1132
Size: 3226 3774
SspI AAT'ATT
Cuts at: 5 52 4924 5
Size: 47 4872 2081
Fragments arranged by size:
4872 2081 47
StuI AGG'CCT
Cuts at: 1517 3899 1517
Size: 2382 4618
StyI C'CwwG_G
Cuts at: 513 1065 2004 3924 4439 513
Size: 552 939 1920 515 3074
Fragments arranged by size:
3074 1920 939 552 515
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2393 2911 3146 5027 5044 5197 5382
Size: 1255 813 518 235 1881 17 153 185
Cuts at: 5382 5827 325
Size: 445 1498
Fragments arranged by size:
1881 1498 1255 813 518 445 235 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2397 2610 3780
Size: 274 80 33 51 1443 420 213 1170
Cuts at: 3780 5246 6958 96
Size: 1466 1712 138
Fragments arranged by size:
1712 1466 1443 1170 420 274 213 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2871 2903 6960 6992 2871
Size: 32 4057 32 2879
Fragments arranged by size:
4057 2879 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7000
XbaI T'CTAG_A
Cuts at: 4336 4336
Size: 7000
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2611 3818 2611
Size: 1207 5793
XhoI C'TCGA_G
Cuts at: 1943 3977 4055 1943
Size: 2034 78 4888
Fragments arranged by size:
4888 2034 78
XmaI C'CCGG_G
Cuts at: 1870 3611 3668 4234 4325 1870
Size: 1741 57 566 91 4545
Fragments arranged by size:
4545 1741 566 91 57
XmnI GAAnn'nnTTC
Cuts at: 5129 5129
Size: 7000
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4808 276
Size: 53 83 186 936 3274 2468
Fragments arranged by size:
3274 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI RsrII SacI SacII SfiI
SrfI SwaI