Restriction map for
pFN21AE2086
(Circular) (Six-base) MAPSORT of: pFN21AE2086.seq Check: 928 from: 1 to: 6985
pFN21AE2086 6985 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2394 4795 278
Size: 53 83 186 936 858 2401 2468
Fragments arranged by size:
2468 2401 936 858 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2034 3882 4328 1937
Size: 97 1848 446 4594
Fragments arranged by size:
4594 1848 446 97
Acc65I G'GTAC_C
Cuts at: 4306 4306
Size: 6985
AclI AA'CG_TT
Cuts at: 1563 5112 5485 1563
Size: 3549 373 3063
Fragments arranged by size:
3549 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2564 3292 5048 6432 6700 1632
Size: 932 728 1756 1384 268 1917
Fragments arranged by size:
1917 1756 1384 932 728 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6788
AflIII A'CryG_T
Cuts at: 1203 5884 6861 1203
Size: 4681 977 1327
Fragments arranged by size:
4681 1327 977
AgeI A'CCGG_T
Cuts at: 2775 5816 2775
Size: 3041 3944
AhdI GACnn_n'nnGTC
Cuts at: 2665 5714 2665
Size: 3049 3936
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6985
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3484 1184
Size: 2300 4685
AlwNI CAG_nnn'CTG
Cuts at: 2845 2963 3126 4728 6300 2845
Size: 118 163 1602 1572 3530
Fragments arranged by size:
3530 1602 1572 163 118
ApaLI G'TGCA_C
Cuts at: 3836 5042 6198 3836
Size: 1206 1156 4623
Fragments arranged by size:
4623 1206 1156
ApoI r'AATT_y
Cuts at: 1493 2483 3524 4294 4520 4672 6781 1493
Size: 990 1041 770 226 152 2109 1697
Fragments arranged by size:
2109 1697 1041 990 770 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5539 160
Size: 872 4507 1606
Fragments arranged by size:
4507 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6985
AvaI C'yCGr_G
Cuts at: 1870 1943 2060 2898 3534 3804 4310 1870
Size: 73 117 838 636 270 506 4545
Fragments arranged by size:
4545 838 636 506 270 117 73
BamHI G'GATC_C
Cuts at: 3672 4092 4315 4682 3672
Size: 420 223 367 5975
Fragments arranged by size:
5975 420 367 223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2506 2559 2571 2778
Size: 325 217 627 351 368 53 12 207
Cuts at: 2778 3725 4306 5761 618
Size: 947 581 1455 1842
Fragments arranged by size:
1842 1455 947 627 581 368 351 325
217 207 53 12
BanII G_rGCy'C
Cuts at: 1391 2028 2088 2336 3065 3264 4304 1391
Size: 637 60 248 729 199 1040 4072
Fragments arranged by size:
4072 1040 729 637 248 199 60
BbeI G_GCGC'C
Cuts at: 2563 2563
Size: 6985
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2153 2692 2813 928
Size: 971 254 539 121 5100
Fragments arranged by size:
5100 971 539 254 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2674 2708 5176 5210 1903
Size: 34 737 34 2468 34 3678
Fragments arranged by size:
3678 2468 737 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2164 4877 6087 2164
Size: 2713 1210 3062
Fragments arranged by size:
3062 2713 1210
BclI T'GATC_A
Cuts at: 1548 2169 3483 1548
Size: 621 1314 5050
Fragments arranged by size:
5050 1314 621
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2687 2918 3329
Size: 107 122 71 631 605 1015 231 411
Cuts at: 3329 5596 136
Size: 2267 1525
Fragments arranged by size:
2267 1525 1015 631 605 411 231 122
107 71
BglII A'GATC_T
Cuts at: 6980 6980
Size: 6985
BlpI GC'TnA_GC
Cuts at: 4220 4275 4402 4220
Size: 55 127 6803
Fragments arranged by size:
6803 127 55
Bme1580I G_kGCm'C
Cuts at: 1790 2511 2576 3840 5046 6202 1790
Size: 721 65 1264 1206 1156 2573
Fragments arranged by size:
2573 1264 1206 1156 721 65
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6985
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3982 4716 5674
Size: 416 53 83 399 24 2565 734 958
Cuts at: 5674 442
Size: 1753
Fragments arranged by size:
2565 1753 958 734 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6985
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2086 2149 2086
Size: 63 6922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2219 2813 3035 3182 3599 3767 4247
Size: 905 75 594 222 147 417 168 480
Cuts at: 4247 5645 1239
Size: 1398 2579
Fragments arranged by size:
2579 1398 905 594 480 417 222 168
147 75
Bpu10I CC'TnA_GC
Cuts at: 2816 3077 2816
Size: 261 6724
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2209 3698 3887 5110 5975 6273 2209
Size: 1489 189 1223 865 298 2921
Fragments arranged by size:
2921 1489 1223 865 298 189
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2789 3524 3707 5648 882
Size: 617 1290 735 183 1941 2219
Fragments arranged by size:
2219 1941 1290 735 617 183
BsaAI yAC'GTr
Cuts at: 493 1204 3022 4776 493
Size: 711 1818 1754 2702
Fragments arranged by size:
2702 1818 1754 711
BsaBI GATnn'nnATC
Cuts at: 6979 6979
Size: 6985
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 2391 2560 2863
Size: 53 83 186 936 570 288 169 303
Cuts at: 2863 2910 3321 3870 4792 5174 5809 275
Size: 47 411 549 922 382 635 1451
Fragments arranged by size:
1451 936 922 635 570 549 411 382
303 288 186 169 83 53 47
BsaWI w'CCGG_w
Cuts at: 2775 3141 3555 5418 5816 6090 6237 6648 2775
Size: 366 414 1863 398 274 147 411 3112
Fragments arranged by size:
3112 1863 414 411 398 366 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6760
Fragments arranged by size:
6760 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4259 1186
Size: 3073 3912
BseYI C'CCAG_C
Cuts at: 3090 4204 6188 6684 3090
Size: 1114 1984 496 3391
Fragments arranged by size:
3391 1984 1114 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3062 3074 3287 1163
Size: 573 1326 12 213 4861
Fragments arranged by size:
4861 1326 573 213 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2982 3493 4435 5196 6224 1540
Size: 275 186 981 511 942 761 1028 2301
Fragments arranged by size:
2301 1028 981 942 761 511 275 186
BsiHKAI G_wGCw'C
Cuts at: 2088 2336 2817 3647 3840 4104 5046 5131 6202
Size: 248 481 830 193 264 942 85 1071
Cuts at: 6202 2088
Size: 2871
Fragments arranged by size:
2871 1071 942 830 481 264 248 193
85
BsiWI C'GTAC_G
Cuts at: 2043 2043
Size: 6985
BsmI GAATG_Cn'
Cuts at: 3897 4496 4589 3897
Size: 599 93 6293
Fragments arranged by size:
6293 599 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2897 4241 2897
Size: 1344 5641
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2088 2336 2511 2576 2817 3065
Size: 399 238 60 248 175 65 241 248
Cuts at: 3065 3264 3647 3840 4104 4304 5046 5131 6202
Size: 199 383 193 264 200 742 85 1071
Cuts at: 6202 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 383 264 248 248
241 238 200 199 193 175 85 65
60
BspEI T'CCGG_A
Cuts at: 3555 6648 3555
Size: 3093 3892
BspHI T'CATG_A
Cuts at: 4874 6604 4874
Size: 1730 5255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3930 4340 844
Size: 3086 410 3489
Fragments arranged by size:
3489 3086 410
BsrBI CCG'CTC
Cuts at: 1394 1823 3081 3193 4872 1394
Size: 429 1258 112 1679 3507
Fragments arranged by size:
3507 1679 1258 429 112
BsrDI GCAATG_nn'
Cuts at: 65 1248 3285 3505 5480 5654 65
Size: 1183 2037 220 1975 174 1396
Fragments arranged by size:
2037 1975 1396 1183 220 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2775 2859 5629 5816 1700
Size: 110 965 84 2770 187 2869
Fragments arranged by size:
2869 2770 965 187 110 84
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6985
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6985
BssSI C'ACGA_G
Cuts at: 5045 6057 5045
Size: 1012 5973
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2845 3052 794
Size: 2051 207 4727
Fragments arranged by size:
4727 2051 207
BstBI TT'CG_AA
Cuts at: 2266 5789 2266
Size: 3523 3462
BstEII G'GTnAC_C
Cuts at: 1184 2203 1184
Size: 1019 5966
BstXI CCAn_nnnn'nTGG
Cuts at: 2617 2677 3415 3457 2617
Size: 60 738 42 6145
Fragments arranged by size:
6145 738 60 42
BstYI r'GATC_y
Cuts at: 1972 3411 3672 4092 4315 4682 5067 5084 6536
Size: 1439 261 420 223 367 385 17 1452
Cuts at: 6536 6622 6634 6980 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1439 420 385 367 346 261
223 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2134 2361 3002 2134
Size: 227 641 6117
Fragments arranged by size:
6117 641 227
BtgI C'CryG_G
Cuts at: 513 1065 2494 3435 3493 3648 3778 4060 4131
Size: 552 1429 941 58 155 130 282 71
Cuts at: 4131 513
Size: 3367
Fragments arranged by size:
3367 1429 941 552 282 155 130 71
58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6246
Fragments arranged by size:
6246 683 56
BtsI GCAGTG_nn'
Cuts at: 2464 2964 4501 5295 5315 2464
Size: 500 1537 794 20 4134
Fragments arranged by size:
4134 1537 794 500 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6985
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6950
DraI TTT'AAA
Cuts at: 4289 4289
Size: 6985
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5992 809
Size: 507 4676 1802
Fragments arranged by size:
4676 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2919 2979 3330
Size: 54 1425 50 275 18 1089 60 351
Cuts at: 3330 3490 3510 3775 4152 4432 5321 6950 8
Size: 160 20 265 377 280 889 1629 43
Fragments arranged by size:
1629 1425 1089 889 377 351 280 275
265 160 60 54 50 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2979 3490 4432 1537
Size: 275 1167 511 942 4090
Fragments arranged by size:
4090 1167 942 511 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2253 4915 1353
Size: 900 2662 3423
Fragments arranged by size:
3423 2662 900
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5572 5946 6092 387
Size: 1064 138 3983 374 146 1280
Fragments arranged by size:
3983 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2086 2334 2086
Size: 248 6737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2219 2564 2813 3035 3182 3292
Size: 393 512 75 345 249 222 147 110
Cuts at: 3292 3599 3767 4247 5048 5645 6432 6700 1239
Size: 307 168 480 801 597 787 268 1524
Fragments arranged by size:
1524 801 787 597 512 480 393 345
307 268 249 222 168 147 110 75
EcoO109I rG'GnC_Cy
Cuts at: 2548 2548
Size: 6985
EcoRI G'AATT_C
Cuts at: 4294 4672 4294
Size: 378 6607
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3721 3721
Size: 6985
FspI TGC'GCA
Cuts at: 5491 5491
Size: 6985
HaeII r_GCGC'y
Cuts at: 1678 2563 2845 2895 6132 1678
Size: 885 282 50 3237 2531
Fragments arranged by size:
3237 2531 885 282 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2176 2208 2583 2615 2651
Size: 32 135 32 604 32 375 32 36
Cuts at: 2651 2683 5626 5658 5700 5732 1373
Size: 32 2943 32 42 32 2626
Fragments arranged by size:
2943 2626 604 375 135 42 36 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2752 4329 4575 669
Size: 988 281 814 1577 246 3079
Fragments arranged by size:
3079 1577 988 814 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6985
HpaI GTT'AAC
Cuts at: 4575 4575
Size: 6985
KasI G'GCGC_C
Cuts at: 2559 2559
Size: 6985
KpnI G_GTAC'C
Cuts at: 4310 4310
Size: 6985
MfeI C'AATT_G
Cuts at: 4584 4584
Size: 6985
MluI A'CGCG_T
Cuts at: 6861 6861
Size: 6985
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6099 6283 1305
Size: 4794 184 2007
Fragments arranged by size:
4794 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2921 3332 3512 4154 10
Size: 54 1425 343 1089 411 180 642 2841
Fragments arranged by size:
2841 1425 1089 642 411 343 180 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2499 2610 4943 5302 5461 518
Size: 648 1333 111 2333 359 159 2042
Fragments arranged by size:
2333 2042 1333 648 359 159 111
MspA1I CmG'CkG
Cuts at: 1606 2197 2519 3312 3394 3590 3780 4089 4204
Size: 591 322 793 82 196 190 309 115
Cuts at: 4204 4401 5078 6226 6471 1606
Size: 197 677 1148 245 2120
Fragments arranged by size:
2120 1148 793 677 591 322 309 245
197 196 190 115 82
NarI GG'CG_CC
Cuts at: 2560 2560
Size: 6985
NcoI C'CATG_G
Cuts at: 513 1065 2494 3435 513
Size: 552 1429 941 4063
Fragments arranged by size:
4063 1429 941 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6985
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6985
NotI GC'GGCC_GC
Cuts at: 4432 4432
Size: 6985
NspI r_CATG'y
Cuts at: 1132 1229 2080 2609 3050 4343 5888 1132
Size: 97 851 529 441 1293 1545 2229
Fragments arranged by size:
2229 1545 1293 851 529 441 97
PasI CC'CwG_GG
Cuts at: 1348 2551 1348
Size: 1203 5782
PciI A'CATG_T
Cuts at: 5884 5884
Size: 6985
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3637 3994 1108
Size: 2529 357 4099
Fragments arranged by size:
4099 2529 357
PfoI T'CCnGG_A
Cuts at: 2121 2640 3414 4095 2121
Size: 519 774 681 5011
Fragments arranged by size:
5011 774 681 519
PmeI GTTT'AAAC
Cuts at: 4289 4289
Size: 6985
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5054 5086 6567 6599 5054
Size: 32 1481 32 5440
Fragments arranged by size:
5440 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6985
PsiI TTA'TAA
Cuts at: 4555 4555
Size: 6985
PstI C_TGCA'G
Cuts at: 830 2805 2952 4257 4337 830
Size: 1975 147 1305 80 3478
Fragments arranged by size:
3478 1975 1305 147 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6985
PvuII CAG'CTG
Cuts at: 3312 4204 3312
Size: 892 6093
SacI G_AGCT'C
Cuts at: 2088 2336 2088
Size: 248 6737
SacII CC_GC'GG
Cuts at: 3781 3781
Size: 6985
SalI G'TCGA_C
Cuts at: 1936 4327 1936
Size: 2391 4594
SapI GCTCTTCn'nnn_
Cuts at: 2253 2253
Size: 6985
SbfI CC_TGCA'GG
Cuts at: 4337 4337
Size: 6985
ScaI AGT'ACT
Cuts at: 4160 5233 6945 4160
Size: 1073 1712 4200
Fragments arranged by size:
4200 1712 1073
SexAI A'CCwGG_T
Cuts at: 2661 3504 2661
Size: 843 6142
SfcI C'TryA_G
Cuts at: 826 1045 2801 2948 4253 4333 5468 6149 6340
Size: 219 1756 147 1305 80 1135 681 191
Cuts at: 6340 826
Size: 1471
Fragments arranged by size:
1756 1471 1305 1135 681 219 191 147
80
SfiI GGCCn_nnn'nGGCC
Cuts at: 2687 2687
Size: 6985
SfoI GGC'GCC
Cuts at: 2561 2561
Size: 6985
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6985
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6985
SmaI CCC'GGG
Cuts at: 1872 2900 3536 3806 4312 1872
Size: 1028 636 270 506 4545
Fragments arranged by size:
4545 1028 636 506 270
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2224 3677 3902 5089 5990 6252
Size: 197 926 281 1453 225 1187 901 262
Cuts at: 6252 820
Size: 1553
Fragments arranged by size:
1553 1453 1187 926 901 281 262 225
197
SnaBI TAC'GTA
Cuts at: 493 4776 493
Size: 4283 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6985
SphI G_CATG'C
Cuts at: 1132 2609 4343 1132
Size: 1477 1734 3774
Fragments arranged by size:
3774 1734 1477
SspI AAT'ATT
Cuts at: 5 52 4909 5
Size: 47 4857 2081
Fragments arranged by size:
4857 2081 47
StuI AGG'CCT
Cuts at: 1517 3764 1517
Size: 2247 4738
StyI C'CwwG_G
Cuts at: 513 1065 2106 2494 2670 3435 4424 513
Size: 552 1041 388 176 765 989 3074
Fragments arranged by size:
3074 1041 989 765 552 388 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5012 5029 5182 5367 5812 325
Size: 1255 3432 17 153 185 445 1498
Fragments arranged by size:
3432 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3633 4158 5231
Size: 274 80 33 51 1443 1656 525 1073
Cuts at: 5231 6943 96
Size: 1712 138
Fragments arranged by size:
1712 1656 1443 1073 525 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4170 4202 6945 6977 4170
Size: 32 2743 32 4178
Fragments arranged by size:
4178 2743 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2726 3385 1316
Size: 1410 659 4916
Fragments arranged by size:
4916 1410 659
XbaI T'CTAG_A
Cuts at: 4321 4321
Size: 6985
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3742 3742
Size: 6985
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6985
XmaI C'CCGG_G
Cuts at: 1870 2898 3534 3804 4310 1870
Size: 1028 636 270 506 4545
Fragments arranged by size:
4545 1028 636 506 270
XmnI GAAnn'nnTTC
Cuts at: 2262 5114 2262
Size: 2852 4133
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2392 4793 276
Size: 53 83 186 936 858 2401 2468
Fragments arranged by size:
2468 2401 936 858 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BstZ17I DraIII EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SanDI SrfI SwaI