Restriction map for
pFN21AE2081
(Circular) (Six-base) MAPSORT of: pFN21AE2081.seq Check: 8489 from: 1 to: 6967
pFN21AE2081 6967 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4777 278
Size: 53 83 186 936 3241 2468
Fragments arranged by size:
3241 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2813 4310 1937
Size: 876 1497 4594
Fragments arranged by size:
4594 1497 876
Acc65I G'GTAC_C
Cuts at: 4288 4288
Size: 6967
AclI AA'CG_TT
Cuts at: 1563 5094 5467 1563
Size: 3531 373 3063
Fragments arranged by size:
3531 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2224 2694 5030 6414 6682 1632
Size: 592 470 2336 1384 268 1917
Fragments arranged by size:
2336 1917 1384 592 470 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6770
AflIII A'CryG_T
Cuts at: 1203 5866 6843 1203
Size: 4663 977 1327
Fragments arranged by size:
4663 1327 977
AgeI A'CCGG_T
Cuts at: 5798 5798
Size: 6967
AhdI GACnn_n'nnGTC
Cuts at: 5696 5696
Size: 6967
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6967
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3743 1184
Size: 2559 4408
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2001 2033 2001
Size: 32 6935
AlwNI CAG_nnn'CTG
Cuts at: 4710 6282 4710
Size: 1572 5395
ApaLI G'TGCA_C
Cuts at: 5024 6180 5024
Size: 1156 5811
ApoI r'AATT_y
Cuts at: 1493 2324 2713 3325 3973 4220 4276 4502 4654
Size: 831 389 612 648 247 56 226 152
Cuts at: 4654 6763 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 831 648 612 389 247 226
152 56
AseI AT'TA_AT
Cuts at: 160 1032 5521 160
Size: 872 4489 1606
Fragments arranged by size:
4489 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6967
AvaI C'yCGr_G
Cuts at: 1870 1943 4292 1870
Size: 73 2349 4545
Fragments arranged by size:
4545 2349 73
BamHI G'GATC_C
Cuts at: 4297 4664 4297
Size: 367 6600
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4288 5743 618
Size: 325 217 627 2501 1455 1842
Fragments arranged by size:
2501 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3112 3896 3959 4286 1391
Size: 1721 784 63 327 4072
Fragments arranged by size:
4072 1721 784 327 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2298 3345 4260 928
Size: 971 399 1047 915 3635
Fragments arranged by size:
3635 1047 971 915 399
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5158 5192 1903
Size: 34 3221 34 3678
Fragments arranged by size:
3678 3221 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2350 2508 4859 6069 2350
Size: 158 2351 1210 3248
Fragments arranged by size:
3248 2351 1210 158
BclI T'GATC_A
Cuts at: 1548 3595 4012 4225 1548
Size: 2047 417 213 4290
Fragments arranged by size:
4290 2047 417 213
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5578 136
Size: 107 122 71 631 605 3906 1525
Fragments arranged by size:
3906 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2987 6962 2987
Size: 3975 2992
BlpI GC'TnA_GC
Cuts at: 4384 4384
Size: 6967
Bme1580I G_kGCm'C
Cuts at: 1790 2105 5028 6184 1790
Size: 315 2923 1156 2573
Fragments arranged by size:
2923 2573 1156 315
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6967
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2803 4698 5656
Size: 416 53 83 399 24 1386 1895 958
Cuts at: 5656 442
Size: 1753
Fragments arranged by size:
1895 1753 1386 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2493 1056
Size: 1437 5530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5627 1239
Size: 4388 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2608 5092 5957 6255 2608
Size: 2484 865 298 3320
Fragments arranged by size:
3320 2484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5630 882
Size: 617 4131 2219
Fragments arranged by size:
4131 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4758 493
Size: 711 3554 2702
Fragments arranged by size:
3554 2702 711
BsaBI GATnn'nnATC
Cuts at: 2412 3046 4056 6961 2412
Size: 634 1010 2905 2418
Fragments arranged by size:
2905 2418 1010 634
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4774 5156 5791 275
Size: 53 83 186 936 3241 382 635 1451
Fragments arranged by size:
3241 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5400 5798 6072 6219 6630 5400
Size: 398 274 147 411 5737
Fragments arranged by size:
5737 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3535 3565 1178
Size: 30 165 30 2132 30 4580
Fragments arranged by size:
4580 2132 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2274 2388 2587 2590 3069 3883 3892 1186
Size: 1088 114 199 3 479 814 9 4261
Fragments arranged by size:
4261 1088 814 479 199 114 9 3
BseYI C'CCAG_C
Cuts at: 2949 6170 6666 2949
Size: 3221 496 3250
Fragments arranged by size:
3250 3221 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6394
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4417 5178 6206 1540
Size: 275 186 2416 761 1028 2301
Fragments arranged by size:
2416 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3112 3896 3959 5028 5113 6184 3112
Size: 784 63 1069 85 1071 3895
Fragments arranged by size:
3895 1071 1069 784 85 63
BsmI GAATG_Cn'
Cuts at: 4478 4571 4478
Size: 93 6874
BsmBI CGTCTCn'nnnn_
Cuts at: 2962 2962
Size: 6967
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 3112 3896 3959 4286 5028 5113
Size: 399 315 1007 784 63 327 742 85
Cuts at: 5113 6184 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1007 784 742 399 327 315
85 63
BspEI T'CCGG_A
Cuts at: 6630 6630
Size: 6967
BspHI T'CATG_A
Cuts at: 3484 4228 4856 6586 3484
Size: 744 628 1730 3865
Fragments arranged by size:
3865 1730 744 628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2071 4322 844
Size: 1227 2251 3489
Fragments arranged by size:
3489 2251 1227
BsrBI CCG'CTC
Cuts at: 1394 1823 4854 1394
Size: 429 3031 3507
Fragments arranged by size:
3507 3031 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2043 5462 5636 65
Size: 1183 795 3419 174 1396
Fragments arranged by size:
3419 1396 1183 795 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5611 5798 1700
Size: 110 3801 187 2869
Fragments arranged by size:
3801 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2135 4201 96
Size: 2039 2066 2862
Fragments arranged by size:
2862 2066 2039
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6967
BssSI C'ACGA_G
Cuts at: 5027 6039 5027
Size: 1012 5955
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3100 794
Size: 2306 4661
BstBI TT'CG_AA
Cuts at: 5771 5771
Size: 6967
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6967
BstYI r'GATC_y
Cuts at: 1972 2832 2987 3041 4148 4297 4664 5049 5066
Size: 860 155 54 1107 149 367 385 17
Cuts at: 5066 6518 6604 6616 6962 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1107 860 385 367 346 155
149 86 54 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5476
Fragments arranged by size:
5476 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6228
Fragments arranged by size:
6228 683 56
BtsI GCAGTG_nn'
Cuts at: 4483 5277 5297 4483
Size: 794 20 6153
Fragments arranged by size:
6153 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6967
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6932
DraI TTT'AAA
Cuts at: 4271 4271
Size: 6967
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5974 809
Size: 507 4658 1802
Fragments arranged by size:
4658 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2340 4414 5303
Size: 54 1425 50 275 18 510 2074 889
Cuts at: 5303 6932 8
Size: 1629 43
Fragments arranged by size:
2074 1629 1425 889 510 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4414 1537
Size: 275 2602 4090
Fragments arranged by size:
4090 2602 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2065 2647 2670 2756 2823 3004 3718 3799
Size: 712 582 23 86 67 181 714 81
Cuts at: 3799 4897 1353
Size: 1098 3423
Fragments arranged by size:
3423 1098 714 712 582 181 86 81
67 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5554 5928 6074 387
Size: 1064 138 3965 374 146 1280
Fragments arranged by size:
3965 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3110 3894 3957 3110
Size: 784 63 6120
Fragments arranged by size:
6120 784 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2224 2694 5030 5627 6414 6682 1239
Size: 393 592 470 2336 597 787 268 1524
Fragments arranged by size:
2336 1524 787 597 592 470 393 268
EcoRI G'AATT_C
Cuts at: 4276 4654 4276
Size: 378 6589
FspI TGC'GCA
Cuts at: 5473 5473
Size: 6967
HaeII r_GCGC'y
Cuts at: 1678 6114 1678
Size: 4436 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2421 2453 2621 2653 2857
Size: 32 135 32 849 32 168 32 204
Cuts at: 2857 2889 3278 3310 4217 4249 5608 5640 5682
Size: 32 389 32 907 32 1359 32 42
Cuts at: 5682 5714 1373
Size: 32 2626
Fragments arranged by size:
2626 1359 907 849 389 204 168 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4311 4557 669
Size: 988 281 2373 246 3079
Fragments arranged by size:
3079 2373 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6967
HpaI GTT'AAC
Cuts at: 4557 4557
Size: 6967
KpnI G_GTAC'C
Cuts at: 4292 4292
Size: 6967
MfeI C'AATT_G
Cuts at: 4566 4566
Size: 6967
MluI A'CGCG_T
Cuts at: 6843 6843
Size: 6967
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6081 6265 1305
Size: 4776 184 2007
Fragments arranged by size:
4776 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2342 10
Size: 54 1425 343 510 4635
Fragments arranged by size:
4635 1425 510 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2406 3234 3489 4203 4925 5284 5443
Size: 648 1240 828 255 714 722 359 159
Cuts at: 5443 518
Size: 2042
Fragments arranged by size:
2042 1240 828 722 714 648 359 255
159
MspA1I CmG'CkG
Cuts at: 1606 4383 5060 6208 6453 1606
Size: 2777 677 1148 245 2120
Fragments arranged by size:
2777 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5476
Fragments arranged by size:
5476 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6967
NheI G'CTAG_C
Cuts at: 1052 2489 1052
Size: 1437 5530
NotI GC'GGCC_GC
Cuts at: 4414 4414
Size: 6967
NspI r_CATG'y
Cuts at: 1132 1229 2115 4325 5870 1132
Size: 97 886 2210 1545 2229
Fragments arranged by size:
2229 2210 1545 886 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6967
PciI A'CATG_T
Cuts at: 5866 5866
Size: 6967
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6967
PfoI T'CCnGG_A
Cuts at: 3635 3635
Size: 6967
PmeI GTTT'AAAC
Cuts at: 4271 4271
Size: 6967
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5036 5068 6549 6581 5036
Size: 32 1481 32 5422
Fragments arranged by size:
5422 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6967
PsiI TTA'TAA
Cuts at: 2184 3678 4537 2184
Size: 1494 859 4614
Fragments arranged by size:
4614 1494 859
PstI C_TGCA'G
Cuts at: 830 4319 830
Size: 3489 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6967
SacI G_AGCT'C
Cuts at: 3112 3896 3959 3112
Size: 784 63 6120
Fragments arranged by size:
6120 784 63
SalI G'TCGA_C
Cuts at: 1936 4309 1936
Size: 2373 4594
SbfI CC_TGCA'GG
Cuts at: 4319 4319
Size: 6967
ScaI AGT'ACT
Cuts at: 5215 6927 5215
Size: 1712 5255
SfcI C'TryA_G
Cuts at: 826 1045 4315 5450 6131 6322 826
Size: 219 3270 1135 681 191 1471
Fragments arranged by size:
3270 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6967
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6967
SmaI CCC'GGG
Cuts at: 1872 4294 1872
Size: 2422 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2623 5071 5972 6234 820
Size: 197 926 680 2448 901 262 1553
Fragments arranged by size:
2448 1553 926 901 680 262 197
SnaBI TAC'GTA
Cuts at: 493 4758 493
Size: 4265 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6967
SphI G_CATG'C
Cuts at: 1132 4325 1132
Size: 3193 3774
SspI AAT'ATT
Cuts at: 5 52 4891 5
Size: 47 4839 2081
Fragments arranged by size:
4839 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6967
StyI C'CwwG_G
Cuts at: 513 1065 2004 3017 4406 513
Size: 552 939 1013 1389 3074
Fragments arranged by size:
3074 1389 1013 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4994 5011 5164 5349 5794 325
Size: 1255 3414 17 153 185 445 1498
Fragments arranged by size:
3414 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2135 3996 4201
Size: 274 80 33 51 1443 158 1861 205
Cuts at: 4201 5213 6925 96
Size: 1012 1712 138
Fragments arranged by size:
1861 1712 1443 1012 274 205 158 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2915 2947 6927 6959 2915
Size: 32 3980 32 2923
Fragments arranged by size:
3980 2923 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6967
XbaI T'CTAG_A
Cuts at: 4303 4303
Size: 6967
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6967
XmaI C'CCGG_G
Cuts at: 1870 4292 1870
Size: 2422 4545
XmnI GAAnn'nnTTC
Cuts at: 3034 3325 5096 3034
Size: 291 1771 4905
Fragments arranged by size:
4905 1771 291
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4775 276
Size: 53 83 186 936 3241 2468
Fragments arranged by size:
3241 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI