Restriction map for
pFN21AE2080
(Circular) (Six-base) MAPSORT of: pFN21AE2080.seq Check: 1306 from: 1 to: 6964
pFN21AE2080 6964 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4774 278
Size: 53 83 186 936 3238 2468
Fragments arranged by size:
3238 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4307 1937
Size: 2370 4594
Acc65I G'GTAC_C
Cuts at: 4285 4285
Size: 6964
AclI AA'CG_TT
Cuts at: 1563 2332 5091 5464 1563
Size: 769 2759 373 3063
Fragments arranged by size:
3063 2759 769 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4173 5027 6411 6679 1632
Size: 2541 854 1384 268 1917
Fragments arranged by size:
2541 1917 1384 854 268
AfeI AGC'GCT
Cuts at: 3106 3106
Size: 6964
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6767
AflIII A'CryG_T
Cuts at: 1203 2782 3723 3825 5863 6840 1203
Size: 1579 941 102 2038 977 1327
Fragments arranged by size:
2038 1579 1327 977 941 102
AgeI A'CCGG_T
Cuts at: 5795 5795
Size: 6964
AhdI GACnn_n'nnGTC
Cuts at: 3664 5693 3664
Size: 2029 4935
AleI CACnn'nnGTG
Cuts at: 1166 3926 1166
Size: 2760 4204
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2052 2738 1184
Size: 868 686 5410
Fragments arranged by size:
5410 868 686
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4118 4150 4118
Size: 32 6932
AlwNI CAG_nnn'CTG
Cuts at: 3232 3375 4707 6279 3232
Size: 143 1332 1572 3917
Fragments arranged by size:
3917 1572 1332 143
ApaI G_GGCC'C
Cuts at: 2132 2813 2132
Size: 681 6283
ApaLI G'TGCA_C
Cuts at: 2032 2064 5021 6177 2032
Size: 32 2957 1156 2819
Fragments arranged by size:
2957 2819 1156 32
ApoI r'AATT_y
Cuts at: 1493 3468 3735 4273 4499 4651 6760 1493
Size: 1975 267 538 226 152 2109 1697
Fragments arranged by size:
2109 1975 1697 538 267 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5518 160
Size: 872 4486 1606
Fragments arranged by size:
4486 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6964
AvaI C'yCGr_G
Cuts at: 1870 1943 2123 4289 1870
Size: 73 180 2166 4545
Fragments arranged by size:
4545 2166 180 73
BamHI G'GATC_C
Cuts at: 3877 4294 4661 3877
Size: 417 367 6180
Fragments arranged by size:
6180 417 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3143 4285 5740 618
Size: 325 217 627 1356 1142 1455 1842
Fragments arranged by size:
1842 1455 1356 1142 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2132 2813 3432 3439 3991 4283 1391
Size: 741 681 619 7 552 292 4072
Fragments arranged by size:
4072 741 681 619 552 292 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4161 928
Size: 971 2262 3731
Fragments arranged by size:
3731 2262 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2023 2057 5155 5189 1903
Size: 34 86 34 3098 34 3678
Fragments arranged by size:
3678 3098 86 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3622 4856 6066 3622
Size: 1234 1210 4520
Fragments arranged by size:
4520 1234 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6964
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2848 2861 5575
Size: 107 122 71 631 605 1176 13 2714
Cuts at: 5575 136
Size: 1525
Fragments arranged by size:
2714 1525 1176 631 605 122 107 71
13
BglII A'GATC_T
Cuts at: 6959 6959
Size: 6964
BlpI GC'TnA_GC
Cuts at: 4381 4381
Size: 6964
Bme1580I G_kGCm'C
Cuts at: 1790 2036 2068 2132 2813 5025 6181 1790
Size: 246 32 64 681 2212 1156 2573
Fragments arranged by size:
2573 2212 1156 681 246 64 32
BmgBI CAC'GTC
Cuts at: 1318 3828 1318
Size: 2510 4454
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4695 5653 442
Size: 416 53 83 399 24 3278 958 1753
Fragments arranged by size:
3278 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2562 2855 1056
Size: 1506 293 5165
Fragments arranged by size:
5165 1506 293
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3061 3061
Size: 6964
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2041 3397 3536 5624 1239
Size: 802 1356 139 2088 2579
Fragments arranged by size:
2579 2088 1356 802 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5089 5954 6252 5089
Size: 865 298 5801
Fragments arranged by size:
5801 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2244 3555 5627 882
Size: 617 745 1311 2072 2219
Fragments arranged by size:
2219 2072 1311 745 617
BsaAI yAC'GTr
Cuts at: 493 1204 2785 4755 493
Size: 711 1581 1970 2702
Fragments arranged by size:
2702 1970 1581 711
BsaBI GATnn'nnATC
Cuts at: 2283 6958 2283
Size: 4675 2289
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2938 4771 5153 5788
Size: 53 83 186 936 1405 1833 382 635
Cuts at: 5788 275
Size: 1451
Fragments arranged by size:
1833 1451 1405 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5397 5795 6069 6216 6627 5397
Size: 398 274 147 411 5734
Fragments arranged by size:
5734 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2975 3005 1178
Size: 30 165 30 1572 30 5137
Fragments arranged by size:
5137 1572 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2171 3037 3394 3573 3902 3995 1186
Size: 985 866 357 179 329 93 4155
Fragments arranged by size:
4155 985 866 357 329 179 93
BseYI C'CCAG_C
Cuts at: 2161 2805 3009 3941 4137 6167 6663 2161
Size: 644 204 932 196 2030 496 2462
Fragments arranged by size:
2462 2030 932 644 496 204 196
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2661 3098 3109 3141 1163
Size: 573 925 437 11 32 4986
Fragments arranged by size:
4986 925 573 437 32 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4222 4414 5175 6203 1540
Size: 275 186 2221 192 761 1028 2301
Fragments arranged by size:
2301 2221 1028 761 275 192 186
BsiHKAI G_wGCw'C
Cuts at: 2036 2068 3047 3609 5025 5110 6181 2036
Size: 32 979 562 1416 85 1071 2819
Fragments arranged by size:
2819 1416 1071 979 562 85 32
BsmI GAATG_Cn'
Cuts at: 2727 4475 4568 2727
Size: 1748 93 5123
Fragments arranged by size:
5123 1748 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3125 4209 3125
Size: 1084 5880
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2068 2132 2813 3047 3432 3439
Size: 399 246 32 64 681 234 385 7
Cuts at: 3439 3609 3991 4283 5025 5110 6181 1391
Size: 170 382 292 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 681 399 385 382 292
246 234 170 85 64 32 7
BspEI T'CCGG_A
Cuts at: 6627 6627
Size: 6964
BspHI T'CATG_A
Cuts at: 4853 6583 4853
Size: 1730 5234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3120 4319 844
Size: 2276 1199 3489
Fragments arranged by size:
3489 2276 1199
BsrBI CCG'CTC
Cuts at: 1394 1823 4851 1394
Size: 429 3028 3507
Fragments arranged by size:
3507 3028 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2396 5459 5633 65
Size: 1183 1148 3063 174 1396
Fragments arranged by size:
3063 1396 1183 1148 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2847 4221 5608 5795 1700
Size: 110 1037 1374 1387 187 2869
Fragments arranged by size:
2869 1387 1374 1037 187 110
BsrGI T'GTAC_A
Cuts at: 96 3523 3822 96
Size: 3427 299 3238
Fragments arranged by size:
3427 3238 299
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6964
BssSI C'ACGA_G
Cuts at: 3100 3816 5024 6036 3100
Size: 716 1208 1012 4028
Fragments arranged by size:
4028 1208 1012 716
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6964
BstBI TT'CG_AA
Cuts at: 5768 5768
Size: 6964
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6964
BstXI CCAn_nnnn'nTGG
Cuts at: 3349 3349
Size: 6964
BstYI r'GATC_y
Cuts at: 1972 2118 3877 4294 4661 5046 5063 6515 6601
Size: 146 1759 417 367 385 17 1452 86
Cuts at: 6601 6613 6959 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1759 1452 417 385 367 346 146
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3912 3912
Size: 6964
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5473
Fragments arranged by size:
5473 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2134 487
Size: 683 56 908 5317
Fragments arranged by size:
5317 908 683 56
BtsI GCAGTG_nn'
Cuts at: 2034 2036 3007 4480 5274 5294 2034
Size: 2 971 1473 794 20 3704
Fragments arranged by size:
3704 1473 971 794 20 2
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6964
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6929
DraI TTT'AAA
Cuts at: 4268 4268
Size: 6964
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3569 5971 809
Size: 507 2253 2402 1802
Fragments arranged by size:
2402 2253 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2845 3057
Size: 54 1425 50 275 18 315 700 212
Cuts at: 3057 3264 4411 5300 6929 8
Size: 207 1147 889 1629 43
Fragments arranged by size:
1629 1425 1147 889 700 315 275 212
207 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4411 1537
Size: 275 2599 4090
Fragments arranged by size:
4090 2599 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2008 2369 3550 3793 3850 4894 1353
Size: 655 361 1181 243 57 1044 3423
Fragments arranged by size:
3423 1181 1044 655 361 243 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5551 5925 6071 387
Size: 1064 138 3962 374 146 1280
Fragments arranged by size:
3962 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 3397 3536 4173 5027 5624 6411
Size: 393 409 1356 139 637 854 597 787
Cuts at: 6411 6679 1239
Size: 268 1524
Fragments arranged by size:
1524 1356 854 787 637 597 409 393
268 139
EcoO109I rG'GnC_Cy
Cuts at: 2128 2809 2810 2840 3372 3783 2128
Size: 681 1 30 532 411 5309
Fragments arranged by size:
5309 681 532 411 30 1
EcoRI G'AATT_C
Cuts at: 3735 4273 4651 3735
Size: 538 378 6048
Fragments arranged by size:
6048 538 378
FspI TGC'GCA
Cuts at: 5470 5470
Size: 6964
HaeII r_GCGC'y
Cuts at: 1678 3108 6111 1678
Size: 1430 3003 2531
Fragments arranged by size:
3003 2531 1430
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2475 2507 3444 3476 3650
Size: 32 135 32 903 32 937 32 174
Cuts at: 3650 3682 3775 3807 3862 3894 5605 5637 5679
Size: 32 93 32 55 32 1711 32 42
Cuts at: 5679 5711 1373
Size: 32 2626
Fragments arranged by size:
2626 1711 937 903 174 135 93 55
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4308 4554 669
Size: 988 281 2370 246 3079
Fragments arranged by size:
3079 2370 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6964
HpaI GTT'AAC
Cuts at: 4554 4554
Size: 6964
KpnI G_GTAC'C
Cuts at: 4289 4289
Size: 6964
MfeI C'AATT_G
Cuts at: 2763 3540 4563 2763
Size: 777 1023 5164
Fragments arranged by size:
5164 1023 777
MluI A'CGCG_T
Cuts at: 6840 6840
Size: 6964
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2665 2689 3497 3957 6078 6262 1305
Size: 1360 24 808 460 2121 184 2007
Fragments arranged by size:
2121 2007 1360 808 460 184 24
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3059 3266 10
Size: 54 1425 343 1227 207 3708
Fragments arranged by size:
3708 1425 1227 343 207 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2720 3926 4922 5281 5440 518
Size: 648 1554 1206 996 359 159 2042
Fragments arranged by size:
2042 1554 1206 996 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2114 3075 3262 4380 5057 6205 6450 1606
Size: 508 961 187 1118 677 1148 245 2120
Fragments arranged by size:
2120 1148 1118 961 677 508 245 187
NaeI GCC'GGC
Cuts at: 2849 2849
Size: 6964
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5473
Fragments arranged by size:
5473 939 552
NdeI CA'TA_TG
Cuts at: 387 3804 387
Size: 3417 3547
NgoMIV G'CCGG_C
Cuts at: 2847 2847
Size: 6964
NheI G'CTAG_C
Cuts at: 1052 2558 2851 1052
Size: 1506 293 5165
Fragments arranged by size:
5165 1506 293
NotI GC'GGCC_GC
Cuts at: 4411 4411
Size: 6964
NspI r_CATG'y
Cuts at: 1132 1229 3524 3727 4322 5867 1132
Size: 97 2295 203 595 1545 2229
Fragments arranged by size:
2295 2229 1545 595 203 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6964
PciI A'CATG_T
Cuts at: 3723 5863 3723
Size: 2140 4824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3375 1108
Size: 2267 4697
PfoI T'CCnGG_A
Cuts at: 2776 2776
Size: 6964
PmeI GTTT'AAAC
Cuts at: 4268 4268
Size: 6964
PmlI CAC'GTG
Cuts at: 2785 2785
Size: 6964
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5033 5065 6546 6578 5033
Size: 32 1481 32 5419
Fragments arranged by size:
5419 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3372 3783 3372
Size: 411 6553
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6964
PsiI TTA'TAA
Cuts at: 4191 4534 4191
Size: 343 6621
PspOMI G'GGCC_C
Cuts at: 2128 2809 2128
Size: 681 6283
PstI C_TGCA'G
Cuts at: 830 2031 4316 830
Size: 1201 2285 3478
Fragments arranged by size:
3478 2285 1201
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6964
SalI G'TCGA_C
Cuts at: 1936 4306 1936
Size: 2370 4594
SapI GCTCTTCn'nnn_
Cuts at: 2008 2008
Size: 6964
SbfI CC_TGCA'GG
Cuts at: 4316 4316
Size: 6964
ScaI AGT'ACT
Cuts at: 5212 6924 5212
Size: 1712 5252
SexAI A'CCwGG_T
Cuts at: 3231 3231
Size: 6964
SfcI C'TryA_G
Cuts at: 826 1045 2027 3693 4312 5447 6128 6319 826
Size: 219 982 1666 619 1135 681 191 1471
Fragments arranged by size:
1666 1471 1135 982 681 619 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6964
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6964
SmaI CCC'GGG
Cuts at: 1872 2125 4291 1872
Size: 253 2166 4545
Fragments arranged by size:
4545 2166 253
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5068 5969 6231 820
Size: 197 926 3125 901 262 1553
Fragments arranged by size:
3125 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4755 493
Size: 4262 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6964
SphI G_CATG'C
Cuts at: 1132 4322 1132
Size: 3190 3774
SspI AAT'ATT
Cuts at: 5 52 3548 4888 5
Size: 47 3496 1340 2081
Fragments arranged by size:
3496 2081 1340 47
StuI AGG'CCT
Cuts at: 1517 3018 1517
Size: 1501 5463
StyI C'CwwG_G
Cuts at: 513 1065 2004 4403 513
Size: 552 939 2399 3074
Fragments arranged by size:
3074 2399 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4991 5008 5161 5346 5791 325
Size: 1255 3411 17 153 185 445 1498
Fragments arranged by size:
3411 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3523 3822 5210
Size: 274 80 33 51 1443 1546 299 1388
Cuts at: 5210 6922 96
Size: 1712 138
Fragments arranged by size:
1712 1546 1443 1388 299 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2623 2655 3741 3773 6924 6956 2623
Size: 32 1086 32 3151 32 2631
Fragments arranged by size:
3151 2631 1086 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6964
XbaI T'CTAG_A
Cuts at: 4300 4300
Size: 6964
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3210 3349 3210
Size: 139 6825
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6964
XmaI C'CCGG_G
Cuts at: 1870 2123 4289 1870
Size: 253 2166 4545
Fragments arranged by size:
4545 2166 253
XmnI GAAnn'nnTTC
Cuts at: 5093 5093
Size: 6964
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4772 276
Size: 53 83 186 936 3238 2468
Fragments arranged by size:
3238 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PspXI PsrI
PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI