Restriction map for
pFN21AE2079
(Circular) (Six-base) MAPSORT of: pFN21AE2079.seq Check: 7924 from: 1 to: 6961
pFN21AE2079 6961 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4771 278
Size: 53 83 186 936 3235 2468
Fragments arranged by size:
3235 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4304 1937
Size: 2367 4594
Acc65I G'GTAC_C
Cuts at: 4282 4282
Size: 6961
AclI AA'CG_TT
Cuts at: 1563 5088 5461 1563
Size: 3525 373 3063
Fragments arranged by size:
3525 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2842 3719 5024 6408 6676 1632
Size: 1210 877 1305 1384 268 1917
Fragments arranged by size:
1917 1384 1305 1210 877 268
AfeI AGC'GCT
Cuts at: 2750 2750
Size: 6961
AflII C'TTAA_G
Cuts at: 820 1017 3890 820
Size: 197 2873 3891
Fragments arranged by size:
3891 2873 197
AflIII A'CryG_T
Cuts at: 1203 3405 5860 6837 1203
Size: 2202 2455 977 1327
Fragments arranged by size:
2455 2202 1327 977
AgeI A'CCGG_T
Cuts at: 5792 5792
Size: 6961
AhdI GACnn_n'nnGTC
Cuts at: 5690 5690
Size: 6961
AleI CACnn'nnGTG
Cuts at: 1166 2191 1166
Size: 1025 5936
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2279 3541 1184
Size: 1095 1262 4604
Fragments arranged by size:
4604 1262 1095
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 6929
AlwNI CAG_nnn'CTG
Cuts at: 2167 2279 3801 4231 4704 6276 2167
Size: 112 1522 430 473 1572 2852
Fragments arranged by size:
2852 1572 1522 473 430 112
ApaI G_GGCC'C
Cuts at: 2012 3490 4156 2012
Size: 1478 666 4817
Fragments arranged by size:
4817 1478 666
ApaLI G'TGCA_C
Cuts at: 5018 6174 5018
Size: 1156 5805
ApoI r'AATT_y
Cuts at: 1493 3599 4246 4270 4496 4648 6757 1493
Size: 2106 647 24 226 152 2109 1697
Fragments arranged by size:
2109 2106 1697 647 226 152 24
AseI AT'TA_AT
Cuts at: 160 1032 5515 160
Size: 872 4483 1606
Fragments arranged by size:
4483 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6961
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 2943 4286 1870
Size: 73 152 848 1343 4545
Fragments arranged by size:
4545 1343 848 152 73
BamHI G'GATC_C
Cuts at: 4291 4658 4291
Size: 367 6594
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 4282 5737 618
Size: 325 217 627 315 2180 1455 1842
Fragments arranged by size:
2180 1842 1455 627 325 315 217
BanII G_rGCy'C
Cuts at: 1391 2012 2131 2381 2610 2910 3490 4156 4280
Size: 621 119 250 229 300 580 666 124
Cuts at: 4280 1391
Size: 4072
Fragments arranged by size:
4072 666 621 580 300 250 229 124
119
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2553 2717 928
Size: 971 654 164 5172
Fragments arranged by size:
5172 971 654 164
BbvCI CC'TCA_GC
Cuts at: 2114 4220 2114
Size: 2106 4855
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2530 2564 5152 5186 1903
Size: 34 593 34 2588 34 3678
Fragments arranged by size:
3678 2588 593 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4853 6063 4853
Size: 1210 5751
BclI T'GATC_A
Cuts at: 1548 2961 1548
Size: 1413 5548
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2163 2406 3773
Size: 107 122 71 631 605 491 243 1367
Cuts at: 3773 5572 136
Size: 1799 1525
Fragments arranged by size:
1799 1525 1367 631 605 491 243 122
107 71
BglII A'GATC_T
Cuts at: 6956 6956
Size: 6961
BlpI GC'TnA_GC
Cuts at: 4378 4378
Size: 6961
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2669 3490 4156 5022 6178 1790
Size: 222 657 821 666 866 1156 2573
Fragments arranged by size:
2573 1156 866 821 666 657 222
BmgBI CAC'GTC
Cuts at: 1318 2650 1318
Size: 1332 5629
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2486 3018 3580
Size: 416 53 83 399 24 1069 532 562
Cuts at: 3580 4692 5650 442
Size: 1112 958 1753
Fragments arranged by size:
1753 1112 1069 958 562 532 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6961
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2851 3946 2851
Size: 1095 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2614 3283 3689 5621 1239
Size: 1375 669 406 1932 2579
Fragments arranged by size:
2579 1932 1375 669 406
Bpu10I CC'TnA_GC
Cuts at: 2114 4220 2114
Size: 2106 4855
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3774 4079 5086 5951 6249 3774
Size: 305 1007 865 298 4486
Fragments arranged by size:
4486 1007 865 305 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2241 3467 3567 3778 5624 882
Size: 617 742 1226 100 211 1846 2219
Fragments arranged by size:
2219 1846 1226 742 617 211 100
BsaAI yAC'GTr
Cuts at: 493 1204 4752 493
Size: 711 3548 2702
Fragments arranged by size:
3548 2702 711
BsaBI GATnn'nnATC
Cuts at: 6955 6955
Size: 6961
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4768 5150 5785 275
Size: 53 83 186 936 3235 382 635 1451
Fragments arranged by size:
3235 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5394 5792 6066 6213 6624 5394
Size: 398 274 147 411 5731
Fragments arranged by size:
5731 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2558 2588 2924 2954 3533
Size: 30 165 30 1155 30 336 30 579
Cuts at: 3533 3563 4124 4154 1178
Size: 30 561 30 3985
Fragments arranged by size:
3985 1155 579 561 336 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2527 3062 1186
Size: 1341 535 5085
Fragments arranged by size:
5085 1341 535
BseYI C'CCAG_C
Cuts at: 2124 2179 2314 4098 6164 6660 2124
Size: 55 135 1784 2066 496 2425
Fragments arranged by size:
2425 2066 1784 496 135 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3323 3739 1163
Size: 573 1587 416 4385
Fragments arranged by size:
4385 1587 573 416
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4411 5172 6200 1540
Size: 275 186 2410 761 1028 2301
Fragments arranged by size:
2410 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2553 5022 5107 6178 2553
Size: 2469 85 1071 3336
Fragments arranged by size:
3336 2469 1071 85
BsmI GAATG_Cn'
Cuts at: 4472 4565 4472
Size: 93 6868
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2131 2381 2553 2610 2669 2910
Size: 399 222 119 250 172 57 59 241
Cuts at: 2910 3490 4156 4280 5022 5107 6178 1391
Size: 580 666 124 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 666 580 399 250 241
222 172 124 119 85 59 57
BspEI T'CCGG_A
Cuts at: 6624 6624
Size: 6961
BspHI T'CATG_A
Cuts at: 2995 3048 4850 6580 2995
Size: 53 1802 1730 3376
Fragments arranged by size:
3376 1802 1730 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2781 3485 4316 844
Size: 1937 704 831 3489
Fragments arranged by size:
3489 1937 831 704
BsrBI CCG'CTC
Cuts at: 1394 1823 2569 4063 4848 1394
Size: 429 746 1494 785 3507
Fragments arranged by size:
3507 1494 785 746 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2344 3523 5456 5630 65
Size: 1183 1096 1179 1933 174 1396
Fragments arranged by size:
1933 1396 1183 1179 1096 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5605 5792 1700
Size: 110 3795 187 2869
Fragments arranged by size:
3795 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6961
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6961
BssSI C'ACGA_G
Cuts at: 4179 5021 6033 4179
Size: 842 1012 5107
Fragments arranged by size:
5107 1012 842
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6961
BstBI TT'CG_AA
Cuts at: 5765 5765
Size: 6961
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6961
BstXI CCAn_nnnn'nTGG
Cuts at: 2687 2753 3241 2687
Size: 66 488 6407
Fragments arranged by size:
6407 488 66
BstYI r'GATC_y
Cuts at: 1972 2853 3186 4291 4658 5043 5060 6512 6598
Size: 881 333 1105 367 385 17 1452 86
Cuts at: 6598 6610 6956 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1105 881 385 367 346 333
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2643 2643
Size: 6961
BtgI C'CryG_G
Cuts at: 513 1065 2004 2119 2309 2328 513
Size: 552 939 115 190 19 5146
Fragments arranged by size:
5146 939 552 190 115 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3549 487
Size: 683 56 2323 3899
Fragments arranged by size:
3899 2323 683 56
BtsI GCAGTG_nn'
Cuts at: 2452 2490 4006 4477 5271 5291 2452
Size: 38 1516 471 794 20 4122
Fragments arranged by size:
4122 1516 794 471 38 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6961
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3130 3165 529
Size: 35 2566 35 4325
Fragments arranged by size:
4325 2566 35 35
DraI TTT'AAA
Cuts at: 4265 4265
Size: 6961
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3506 5968 809
Size: 507 2190 2462 1802
Fragments arranged by size:
2462 2190 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2449 4408 5297
Size: 54 1425 50 275 18 619 1959 889
Cuts at: 5297 6926 8
Size: 1629 43
Fragments arranged by size:
1959 1629 1425 889 619 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4408 1537
Size: 275 2596 4090
Fragments arranged by size:
4090 2596 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3200 4205 4891 1353
Size: 1847 1005 686 3423
Fragments arranged by size:
3423 1847 1005 686
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5548 5922 6068 387
Size: 1064 138 3959 374 146 1280
Fragments arranged by size:
3959 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2614 2842 3283 3689 3719 5024 5621
Size: 393 982 228 441 406 30 1305 597
Cuts at: 5621 6408 6676 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1305 982 787 597 441 406 393
268 228 30
EcoNI CCTnn'n_nnAGG
Cuts at: 4219 4219
Size: 6961
EcoO109I rG'GnC_Cy
Cuts at: 2164 2319 3486 3487 3508 4005 4152 4153 4168
Size: 155 1167 1 21 497 147 1 15
Cuts at: 4168 2164
Size: 4957
Fragments arranged by size:
4957 1167 497 155 147 21 15 1
1
EcoRI G'AATT_C
Cuts at: 3599 4270 4648 3599
Size: 671 378 5912
Fragments arranged by size:
5912 671 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3620 3620
Size: 6961
FspI TGC'GCA
Cuts at: 5467 5467
Size: 6961
HaeII r_GCGC'y
Cuts at: 1678 2752 2835 6108 1678
Size: 1074 83 3273 2531
Fragments arranged by size:
3273 2531 1074 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3099 3122 3131 3154 3269
Size: 32 135 32 1527 23 9 23 115
Cuts at: 3269 3301 3505 3537 3566 3598 4087 4119 4124
Size: 32 204 32 29 32 489 32 5
Cuts at: 4124 4156 5602 5634 5676 5708 1373
Size: 32 1446 32 42 32 2626
Fragments arranged by size:
2626 1527 1446 489 204 135 115 42
32 32 32 32 32 32 32 32
32 29 23 23 9 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3172 4305 4551 669
Size: 988 281 1234 1133 246 3079
Fragments arranged by size:
3079 1234 1133 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6961
HpaI GTT'AAC
Cuts at: 4551 4551
Size: 6961
KpnI G_GTAC'C
Cuts at: 4286 4286
Size: 6961
MfeI C'AATT_G
Cuts at: 4560 4560
Size: 6961
MluI A'CGCG_T
Cuts at: 6837 6837
Size: 6961
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2781 3130 3522 6075 6259 1305
Size: 1476 349 392 2553 184 2007
Fragments arranged by size:
2553 2007 1476 392 349 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2451 10
Size: 54 1425 343 619 4520
Fragments arranged by size:
4520 1425 619 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2191 3000 3239 3816 4919 5278 5437
Size: 648 1025 809 239 577 1103 359 159
Cuts at: 5437 518
Size: 2042
Fragments arranged by size:
2042 1103 1025 809 648 577 359 239
159
MspA1I CmG'CkG
Cuts at: 1606 2205 2429 3167 4377 5054 6202 6447 1606
Size: 599 224 738 1210 677 1148 245 2120
Fragments arranged by size:
2120 1210 1148 738 677 599 245 224
NcoI C'CATG_G
Cuts at: 513 1065 2004 2119 513
Size: 552 939 115 5355
Fragments arranged by size:
5355 939 552 115
NdeI CA'TA_TG
Cuts at: 387 3357 387
Size: 2970 3991
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6961
NotI GC'GGCC_GC
Cuts at: 4408 4408
Size: 6961
NspI r_CATG'y
Cuts at: 1132 1229 2413 2686 3113 3311 3409 3449 4210
Size: 97 1184 273 427 198 98 40 761
Cuts at: 4210 4319 5864 1132
Size: 109 1545 2229
Fragments arranged by size:
2229 1545 1184 761 427 273 198 109
98 97 40
PasI CC'CwG_GG
Cuts at: 1348 2161 2662 3629 1348
Size: 813 501 967 4680
Fragments arranged by size:
4680 967 813 501
PciI A'CATG_T
Cuts at: 3405 5860 3405
Size: 2455 4506
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6961
PfoI T'CCnGG_A
Cuts at: 2778 3666 2778
Size: 888 6073
PmeI GTTT'AAAC
Cuts at: 4265 4265
Size: 6961
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2956 5030 5062 6543 6575 2924
Size: 32 2074 32 1481 32 3310
Fragments arranged by size:
3310 2074 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2319 3508 4005 4168 2319
Size: 1189 497 163 5112
Fragments arranged by size:
5112 1189 497 163
PshAI GACnn'nnGTC
Cuts at: 1613 3965 1613
Size: 2352 4609
PsiI TTA'TAA
Cuts at: 4531 4531
Size: 6961
PspOMI G'GGCC_C
Cuts at: 2008 3486 4152 2008
Size: 1478 666 4817
Fragments arranged by size:
4817 1478 666
PstI C_TGCA'G
Cuts at: 830 3324 4313 830
Size: 2494 989 3478
Fragments arranged by size:
3478 2494 989
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6961
PvuII CAG'CTG
Cuts at: 2429 3167 2429
Size: 738 6223
SalI G'TCGA_C
Cuts at: 1936 4303 1936
Size: 2367 4594
SanDI GG'GwC_CC
Cuts at: 4005 4005
Size: 6961
SbfI CC_TGCA'GG
Cuts at: 4313 4313
Size: 6961
ScaI AGT'ACT
Cuts at: 5209 6921 5209
Size: 1712 5249
SexAI A'CCwGG_T
Cuts at: 3342 3342
Size: 6961
SfcI C'TryA_G
Cuts at: 826 1045 3320 3802 4309 5444 6125 6316 826
Size: 219 2275 482 507 1135 681 191 1471
Fragments arranged by size:
2275 1471 1135 681 507 482 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6961
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6961
SmaI CCC'GGG
Cuts at: 1872 2945 4288 1872
Size: 1073 1343 4545
Fragments arranged by size:
4545 1343 1073
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3789 3890 4058 5065 5966 6228
Size: 197 926 1846 101 168 1007 901 262
Cuts at: 6228 820
Size: 1553
Fragments arranged by size:
1846 1553 1007 926 901 262 197 168
101
SnaBI TAC'GTA
Cuts at: 493 4752 493
Size: 4259 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6961
SphI G_CATG'C
Cuts at: 1132 3311 4210 4319 1132
Size: 2179 899 109 3774
Fragments arranged by size:
3774 2179 899 109
SspI AAT'ATT
Cuts at: 5 52 4885 5
Size: 47 4833 2081
Fragments arranged by size:
4833 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6961
StyI C'CwwG_G
Cuts at: 513 1065 2004 2119 4120 4400 513
Size: 552 939 115 2001 280 3074
Fragments arranged by size:
3074 2001 939 552 280 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2321 2720 3137 3743 4988 5005 5158
Size: 1255 741 399 417 606 1245 17 153
Cuts at: 5158 5343 5788 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1245 741 606 445 417 399
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5207 6919 96
Size: 274 80 33 51 1443 3230 1712 138
Fragments arranged by size:
3230 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2558 2590 3901 3933 6921 6953 2558
Size: 32 1311 32 2988 32 2566
Fragments arranged by size:
2988 2566 1311 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2519 1316
Size: 1203 5758
XbaI T'CTAG_A
Cuts at: 4297 4297
Size: 6961
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3394 3394
Size: 6961
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6961
XmaI C'CCGG_G
Cuts at: 1870 2943 4286 1870
Size: 1073 1343 4545
Fragments arranged by size:
4545 1343 1073
XmnI GAAnn'nnTTC
Cuts at: 5090 5090
Size: 6961
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4769 276
Size: 53 83 186 936 3235 2468
Fragments arranged by size:
3235 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SapI SfiI SfoI SrfI SwaI