Restriction map for
pFN21AE2075
(Circular) (Six-base) MAPSORT of: pFN21AE2075.seq Check: 1626 from: 1 to: 6922
pFN21AE2075 6922 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4732 278
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2430 2661 2755 4265 1937
Size: 493 231 94 1510 4594
Fragments arranged by size:
4594 1510 493 231 94
Acc65I G'GTAC_C
Cuts at: 4243 4243
Size: 6922
AclI AA'CG_TT
Cuts at: 1563 5049 5422 1563
Size: 3486 373 3063
Fragments arranged by size:
3486 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3929 4985 6369 6637 1632
Size: 2297 1056 1384 268 1917
Fragments arranged by size:
2297 1917 1384 1056 268
AflII C'TTAA_G
Cuts at: 820 1017 3326 820
Size: 197 2309 4416
Fragments arranged by size:
4416 2309 197
AflIII A'CryG_T
Cuts at: 1203 3005 3875 5821 6798 1203
Size: 1802 870 1946 977 1327
Fragments arranged by size:
1946 1802 1327 977 870
AgeI A'CCGG_T
Cuts at: 5753 5753
Size: 6922
AhdI GACnn_n'nnGTC
Cuts at: 2515 5651 2515
Size: 3136 3786
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6922
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6922
AlwNI CAG_nnn'CTG
Cuts at: 2423 4665 6237 2423
Size: 2242 1572 3108
Fragments arranged by size:
3108 2242 1572
ApaLI G'TGCA_C
Cuts at: 2287 3151 4979 6135 2287
Size: 864 1828 1156 3074
Fragments arranged by size:
3074 1828 1156 864
ApoI r'AATT_y
Cuts at: 1493 2492 2865 3482 4231 4457 4609 6718 1493
Size: 999 373 617 749 226 152 2109 1697
Fragments arranged by size:
2109 1697 999 749 617 373 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2921 3785 5476 160
Size: 872 1889 864 1691 1606
Fragments arranged by size:
1889 1691 1606 872 864
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
AvaI C'yCGr_G
Cuts at: 1870 1943 4247 1870
Size: 73 2304 4545
Fragments arranged by size:
4545 2304 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3534 3567 3534
Size: 33 6889
BamHI G'GATC_C
Cuts at: 2356 4252 4619 2356
Size: 1896 367 4659
Fragments arranged by size:
4659 1896 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3956 4243 5698 618
Size: 325 217 627 2169 287 1455 1842
Fragments arranged by size:
2169 1842 1455 627 325 287 217
BanII G_rGCy'C
Cuts at: 1391 3119 3204 3263 4241 1391
Size: 1728 85 59 978 4072
Fragments arranged by size:
4072 1728 978 85 59
BbeI G_GCGC'C
Cuts at: 3960 3960
Size: 6922
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5951
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5113 5147 1903
Size: 34 50 34 3092 34 3678
Fragments arranged by size:
3678 3092 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3361 4814 6024 3361
Size: 1453 1210 4259
Fragments arranged by size:
4259 1453 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6922
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5533 136
Size: 107 122 71 631 605 3861 1525
Fragments arranged by size:
3861 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2337 2952 6917 2337
Size: 615 3965 2342
Fragments arranged by size:
3965 2342 615
BlpI GC'TnA_GC
Cuts at: 4339 4339
Size: 6922
Bme1580I G_kGCm'C
Cuts at: 1790 2291 2628 3155 4983 6139 1790
Size: 501 337 527 1828 1156 2573
Fragments arranged by size:
2573 1828 1156 527 501 337
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6922
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3973 4653 5611
Size: 416 53 83 399 24 2556 680 958
Cuts at: 5611 442
Size: 1753
Fragments arranged by size:
2556 1753 958 680 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6922
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2359 2397 2492 3223 2359
Size: 38 95 731 6058
Fragments arranged by size:
6058 731 95 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2405 2468 2519 2748 2797 3064 3332 3365
Size: 1166 63 51 229 49 267 268 33
Cuts at: 3365 3596 5582 1239
Size: 231 1986 2579
Fragments arranged by size:
2579 1986 1166 268 267 231 229 63
51 49 33
Bpu10I CC'TnA_GC
Cuts at: 3113 4095 3113
Size: 982 5940
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2055 3628 5047 5912 6210 2055
Size: 1573 1419 865 298 2767
Fragments arranged by size:
2767 1573 1419 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2081 5585 882
Size: 617 582 3504 2219
Fragments arranged by size:
3504 2219 617 582
BsaAI yAC'GTr
Cuts at: 493 1204 4713 493
Size: 711 3509 2702
Fragments arranged by size:
3509 2702 711
BsaBI GATnn'nnATC
Cuts at: 6916 6916
Size: 6922
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3957 4729 5111 5746
Size: 53 83 186 936 2424 772 382 635
Cuts at: 5746 275
Size: 1451
Fragments arranged by size:
2424 1451 936 772 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5355 5753 6027 6174 6585 5355
Size: 398 274 147 411 5692
Fragments arranged by size:
5692 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2536 2566 2979 3009 3843
Size: 30 165 30 1133 30 413 30 834
Cuts at: 3843 3873 3921 3951 1178
Size: 30 48 30 4149
Fragments arranged by size:
4149 1133 834 413 165 48 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3097 1186
Size: 1911 5011
BseYI C'CCAG_C
Cuts at: 2390 6125 6621 2390
Size: 3735 496 2691
Fragments arranged by size:
3735 2691 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6349
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4372 5133 6161 1540
Size: 275 186 2371 761 1028 2301
Fragments arranged by size:
2371 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2291 3155 3204 3263 4110 4983 5068 6139 2291
Size: 864 49 59 847 873 85 1071 3074
Fragments arranged by size:
3074 1071 873 864 847 85 59 49
BsmI GAATG_Cn'
Cuts at: 3691 4433 4526 3691
Size: 742 93 6087
Fragments arranged by size:
6087 742 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2137 2137
Size: 6922
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2291 2628 3119 3155 3204 3263 4110
Size: 399 501 337 491 36 49 59 847
Cuts at: 4110 4241 4983 5068 6139 1391
Size: 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 847 742 501 491 399 337
131 85 59 49 36
BspEI T'CCGG_A
Cuts at: 6585 6585
Size: 6922
BspHI T'CATG_A
Cuts at: 2478 4811 6541 2478
Size: 2333 1730 2859
Fragments arranged by size:
2859 2333 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4144 4277 844
Size: 3300 133 3489
Fragments arranged by size:
3489 3300 133
BsrBI CCG'CTC
Cuts at: 1394 1823 3072 4809 1394
Size: 429 1249 1737 3507
Fragments arranged by size:
3507 1737 1249 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5417 5591 65
Size: 1183 4169 174 1396
Fragments arranged by size:
4169 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3306 5566 5753 1700
Size: 110 1496 2260 187 2869
Fragments arranged by size:
2869 2260 1496 187 110
BsrGI T'GTAC_A
Cuts at: 96 3872 96
Size: 3776 3146
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6922
BssSI C'ACGA_G
Cuts at: 3248 4103 4982 5994 3248
Size: 855 879 1012 4176
Fragments arranged by size:
4176 1012 879 855
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6922
BstBI TT'CG_AA
Cuts at: 3486 5726 3486
Size: 2240 4682
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6922
BstXI CCAn_nnnn'nTGG
Cuts at: 2536 3897 2536
Size: 1361 5561
BstYI r'GATC_y
Cuts at: 1972 2337 2356 2952 3220 4252 4619 5004 5021
Size: 365 19 596 268 1032 367 385 17
Cuts at: 5021 6473 6559 6571 6917 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1032 596 385 367 365 346
268 86 19 17 12
BstZ17I GTA'TAC
Cuts at: 2431 2431
Size: 6922
Bsu36I CC'TnA_GG
Cuts at: 2352 2352
Size: 6922
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6183
Fragments arranged by size:
6183 683 56
BtsI GCAGTG_nn'
Cuts at: 4438 5232 5252 4438
Size: 794 20 6108
Fragments arranged by size:
6108 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6922
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6887
DraI TTT'AAA
Cuts at: 2080 4226 2080
Size: 2146 4776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5929 809
Size: 507 4613 1802
Fragments arranged by size:
4613 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4369 5258 6887
Size: 54 1425 50 275 18 2539 889 1629
Cuts at: 6887 8
Size: 43
Fragments arranged by size:
2539 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4369 1537
Size: 275 2557 4090
Fragments arranged by size:
4090 2557 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2477 4060 4852 1353
Size: 1124 1583 792 3423
Fragments arranged by size:
3423 1583 1124 792
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5509 5883 6029 387
Size: 1064 138 3920 374 146 1280
Fragments arranged by size:
3920 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3202 3261 3202
Size: 59 6863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2405 2468 2519 2748 2797 3064 3332
Size: 393 773 63 51 229 49 267 268
Cuts at: 3332 3365 3596 3929 4985 5582 6369 6637 1239
Size: 33 231 333 1056 597 787 268 1524
Fragments arranged by size:
1524 1056 787 773 597 393 333 268
268 267 231 229 63 51 49 33
EcoRI G'AATT_C
Cuts at: 3482 4231 4609 3482
Size: 749 378 5795
Fragments arranged by size:
5795 749 378
EcoRV GAT'ATC
Cuts at: 2108 2108
Size: 6922
FspI TGC'GCA
Cuts at: 5428 5428
Size: 6922
HaeII r_GCGC'y
Cuts at: 1678 3960 6069 1678
Size: 2282 2109 2531
Fragments arranged by size:
2531 2282 2109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 2182 2214 2236
Size: 32 135 32 545 32 33 32 22
Cuts at: 2236 2268 2501 2533 2962 2979 2994 3011 3365
Size: 32 233 32 429 17 15 17 354
Cuts at: 3365 3397 3843 3875 5563 5595 5637 5669 1373
Size: 32 446 32 1688 32 42 32 2626
Fragments arranged by size:
2626 1688 545 446 429 354 233 135
42 33 32 32 32 32 32 32
32 32 32 32 22 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 4266 4512 669
Size: 988 281 1065 1263 246 3079
Fragments arranged by size:
3079 1263 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2073 748
Size: 1325 5597
HpaI GTT'AAC
Cuts at: 4512 4512
Size: 6922
KasI G'GCGC_C
Cuts at: 3956 3956
Size: 6922
KpnI G_GTAC'C
Cuts at: 4247 4247
Size: 6922
MfeI C'AATT_G
Cuts at: 4521 4521
Size: 6922
MluI A'CGCG_T
Cuts at: 6798 6798
Size: 6922
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6036 6220 1305
Size: 4731 184 2007
Fragments arranged by size:
4731 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5100
Fragments arranged by size:
5100 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2373 2607 3471 4145 4880 5239 5398
Size: 648 1207 234 864 674 735 359 159
Cuts at: 5398 518
Size: 2042
Fragments arranged by size:
2042 1207 864 735 674 648 359 234
159
MspA1I CmG'CkG
Cuts at: 1606 4338 5015 6163 6408 1606
Size: 2732 677 1148 245 2120
Fragments arranged by size:
2732 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 3957 3957
Size: 6922
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6370
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6922
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6922
NotI GC'GGCC_GC
Cuts at: 4369 4369
Size: 6922
NspI r_CATG'y
Cuts at: 1132 1229 3009 3606 3879 4280 5825 1132
Size: 97 1780 597 273 401 1545 2229
Fragments arranged by size:
2229 1780 1545 597 401 273 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6922
PciI A'CATG_T
Cuts at: 3005 3875 5821 3005
Size: 870 1946 4106
Fragments arranged by size:
4106 1946 870
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2207 1108
Size: 1099 5823
PfoI T'CCnGG_A
Cuts at: 3041 3573 3041
Size: 532 6390
PmeI GTTT'AAAC
Cuts at: 4226 4226
Size: 6922
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2261 2293 3125 3157 4991 5023 6504 6536 2261
Size: 32 832 32 1834 32 1481 32 2647
Fragments arranged by size:
2647 1834 1481 832 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6922
PsiI TTA'TAA
Cuts at: 4492 4492
Size: 6922
PstI C_TGCA'G
Cuts at: 830 4274 830
Size: 3444 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6922
SacI G_AGCT'C
Cuts at: 3204 3263 3204
Size: 59 6863
SalI G'TCGA_C
Cuts at: 1936 4264 1936
Size: 2328 4594
SbfI CC_TGCA'GG
Cuts at: 4274 4274
Size: 6922
ScaI AGT'ACT
Cuts at: 3939 5170 6882 3939
Size: 1231 1712 3979
Fragments arranged by size:
3979 1712 1231
SfcI C'TryA_G
Cuts at: 826 1045 2804 3668 3948 4270 5405 6086 6277
Size: 219 1759 864 280 322 1135 681 191
Cuts at: 6277 826
Size: 1471
Fragments arranged by size:
1759 1471 1135 864 681 322 280 219
191
SfoI GGC'GCC
Cuts at: 3958 3958
Size: 6922
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6922
SmaI CCC'GGG
Cuts at: 1872 4249 1872
Size: 2377 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2070 3326 3607 5026 5927 6189
Size: 197 926 127 1256 281 1419 901 262
Cuts at: 6189 820
Size: 1553
Fragments arranged by size:
1553 1419 1256 926 901 281 262 197
127
SnaBI TAC'GTA
Cuts at: 493 4713 493
Size: 4220 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6922
SphI G_CATG'C
Cuts at: 1132 4280 1132
Size: 3148 3774
SspI AAT'ATT
Cuts at: 5 52 4846 5
Size: 47 4794 2081
Fragments arranged by size:
4794 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6922
StyI C'CwwG_G
Cuts at: 513 1065 2029 4361 513
Size: 552 964 2332 3074
Fragments arranged by size:
3074 2332 964 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2686 4949 4966 5119 5304 5749 325
Size: 1255 1106 2263 17 153 185 445 1498
Fragments arranged by size:
2263 1498 1255 1106 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3872 3937 5168
Size: 274 80 33 51 1443 1895 65 1231
Cuts at: 5168 6880 96
Size: 1712 138
Fragments arranged by size:
1895 1712 1443 1231 274 138 80 65
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2316 2348 2856 2888 3180 3212 6882 6914 2316
Size: 32 508 32 292 32 3670 32 2324
Fragments arranged by size:
3670 2324 508 292 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2129 2993 3857 1316
Size: 813 864 864 4381
Fragments arranged by size:
4381 864 864 813
XbaI T'CTAG_A
Cuts at: 2102 3385 4258 2102
Size: 1283 873 4766
Fragments arranged by size:
4766 1283 873
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2175 2772 3039 2175
Size: 597 267 6058
Fragments arranged by size:
6058 597 267
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6922
XmaI C'CCGG_G
Cuts at: 1870 4247 1870
Size: 2377 4545
XmnI GAAnn'nnTTC
Cuts at: 5051 5051
Size: 6922
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4730 276
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
BsiWI DraIII EcoNI EcoO109I FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI