Restriction map for
pFN21AE2072
(Circular) (Six-base) MAPSORT of: pFN21AE2072.seq Check: 3749 from: 1 to: 6913
pFN21AE2072 6913 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4723 278
Size: 53 83 186 936 3187 2468
Fragments arranged by size:
3187 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3254 4256 1937
Size: 1317 1002 4594
Fragments arranged by size:
4594 1317 1002
Acc65I G'GTAC_C
Cuts at: 4234 4234
Size: 6913
AclI AA'CG_TT
Cuts at: 1563 5040 5413 1563
Size: 3477 373 3063
Fragments arranged by size:
3477 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3611 4092 4976 6360 6628 1632
Size: 1979 481 884 1384 268 1917
Fragments arranged by size:
1979 1917 1384 884 481 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6716
AflIII A'CryG_T
Cuts at: 1203 5812 6789 1203
Size: 4609 977 1327
Fragments arranged by size:
4609 1327 977
AgeI A'CCGG_T
Cuts at: 2721 5744 2721
Size: 3023 3890
AhdI GACnn_n'nnGTC
Cuts at: 5642 5642
Size: 6913
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6913
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6913
AlwNI CAG_nnn'CTG
Cuts at: 3833 3954 4014 4024 4656 6228 3833
Size: 121 60 10 632 1572 4518
Fragments arranged by size:
4518 1572 632 121 60 10
ApaLI G'TGCA_C
Cuts at: 4970 6126 4970
Size: 1156 5757
ApoI r'AATT_y
Cuts at: 1493 3388 3758 4222 4448 4600 6709 1493
Size: 1895 370 464 226 152 2109 1697
Fragments arranged by size:
2109 1895 1697 464 370 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2945 5467 160
Size: 872 1913 2522 1606
Fragments arranged by size:
2522 1913 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6913
AvaI C'yCGr_G
Cuts at: 1870 1943 4238 1870
Size: 73 2295 4545
Fragments arranged by size:
4545 2295 73
BamHI G'GATC_C
Cuts at: 2329 4243 4610 2329
Size: 1914 367 4632
Fragments arranged by size:
4632 1914 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4234 5689 618
Size: 325 217 627 2447 1455 1842
Fragments arranged by size:
2447 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4232 1391
Size: 2841 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3039 3198 3286 3599 4197 928
Size: 971 1140 159 88 313 598 3644
Fragments arranged by size:
3644 1140 971 598 313 159 88
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2215 2249 5104 5138 1903
Size: 34 278 34 2855 34 3678
Fragments arranged by size:
3678 2855 278 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4201 4805 6015 4201
Size: 604 1210 5099
Fragments arranged by size:
5099 1210 604
BclI T'GATC_A
Cuts at: 1548 3349 1548
Size: 1801 5112
BfrBI ATG'CAT
Cuts at: 2950 3102 2950
Size: 152 6761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5524 136
Size: 107 122 71 631 605 3852 1525
Fragments arranged by size:
3852 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6908 6908
Size: 6913
BlpI GC'TnA_GC
Cuts at: 4330 4330
Size: 6913
Bme1580I G_kGCm'C
Cuts at: 1790 2385 4974 6130 1790
Size: 595 2589 1156 2573
Fragments arranged by size:
2589 2573 1156 595
BmgBI CAC'GTC
Cuts at: 1318 3211 1318
Size: 1893 5020
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3244 4644 5602
Size: 416 53 83 399 24 1827 1400 958
Cuts at: 5602 442
Size: 1753
Fragments arranged by size:
1827 1753 1400 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6913
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2143 2605 2693 3378 5573 1239
Size: 904 462 88 685 2195 2579
Fragments arranged by size:
2579 2195 904 685 462 88
Bpu10I CC'TnA_GC
Cuts at: 2143 3698 3918 2143
Size: 1555 220 5138
Fragments arranged by size:
5138 1555 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2095 5038 5903 6201 2095
Size: 2943 865 298 2807
Fragments arranged by size:
2943 2807 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2699 3123 5576 882
Size: 617 1200 424 2453 2219
Fragments arranged by size:
2453 2219 1200 617 424
BsaAI yAC'GTr
Cuts at: 493 1204 4704 493
Size: 711 3500 2702
Fragments arranged by size:
3500 2702 711
BsaBI GATnn'nnATC
Cuts at: 6907 6907
Size: 6913
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4720 5102 5737 275
Size: 53 83 186 936 3187 382 635 1451
Fragments arranged by size:
3187 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2721 5346 5744 6018 6165 6576 2721
Size: 2625 398 274 147 411 3058
Fragments arranged by size:
3058 2625 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2122 2152 1178
Size: 30 165 30 719 30 5939
Fragments arranged by size:
5939 719 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6913
BseYI C'CCAG_C
Cuts at: 2351 2596 6116 6612 2351
Size: 245 3520 496 2652
Fragments arranged by size:
3520 2652 496 245
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3215 3711 1163
Size: 573 1479 496 4365
Fragments arranged by size:
4365 1479 573 496
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3658 4363 5124 6152 1540
Size: 275 186 1657 705 761 1028 2301
Fragments arranged by size:
2301 1657 1028 761 705 275 186
BsiHKAI G_wGCw'C
Cuts at: 4974 5059 6130 4974
Size: 85 1071 5757
Fragments arranged by size:
5757 1071 85
BsmI GAATG_Cn'
Cuts at: 2766 3660 4424 4517 2766
Size: 894 764 93 5162
Fragments arranged by size:
5162 894 764 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2385 4232 4974 5059 6130 1391
Size: 399 595 1847 742 85 1071 2174
Fragments arranged by size:
2174 1847 1071 742 595 399 85
BspEI T'CCGG_A
Cuts at: 6576 6576
Size: 6913
BspHI T'CATG_A
Cuts at: 2916 3489 4802 6532 2916
Size: 573 1313 1730 3297
Fragments arranged by size:
3297 1730 1313 573
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4268 844
Size: 3424 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3784 4800 1394
Size: 429 1961 1016 3507
Fragments arranged by size:
3507 1961 1016 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2256 2667 3740 4179 5408 5582 65
Size: 1183 1008 411 1073 439 1229 174 1396
Fragments arranged by size:
1396 1229 1183 1073 1008 439 411 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2721 5557 5744 1700
Size: 110 911 2836 187 2869
Fragments arranged by size:
2869 2836 911 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6913
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6913
BssSI C'ACGA_G
Cuts at: 4973 5985 4973
Size: 1012 5901
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6913
BstBI TT'CG_AA
Cuts at: 5717 5717
Size: 6913
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6913
BstXI CCAn_nnnn'nTGG
Cuts at: 2930 3028 3718 2930
Size: 98 690 6125
Fragments arranged by size:
6125 690 98
BstYI r'GATC_y
Cuts at: 1972 2329 2865 3309 4243 4610 4995 5012 6464
Size: 357 536 444 934 367 385 17 1452
Cuts at: 6464 6550 6562 6908 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 934 536 444 385 367 357
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2202 2202
Size: 6913
BtgI C'CryG_G
Cuts at: 513 1065 4203 513
Size: 552 3138 3223
Fragments arranged by size:
3223 3138 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2462 2502 487
Size: 683 56 1236 40 4898
Fragments arranged by size:
4898 1236 683 56 40
BtsI GCAGTG_nn'
Cuts at: 2231 4429 5223 5243 2231
Size: 2198 794 20 3901
Fragments arranged by size:
3901 2198 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6913
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6878
DraI TTT'AAA
Cuts at: 4217 4217
Size: 6913
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2722 3869 5920 809
Size: 507 1406 1147 2051 1802
Fragments arranged by size:
2051 1802 1406 1147 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3018 4360 5249
Size: 54 1425 50 275 18 1188 1342 889
Cuts at: 5249 6878 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1342 1188 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4360 1537
Size: 275 2548 4090
Fragments arranged by size:
4090 2548 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2350 2467 2472 4843 1353
Size: 997 117 5 2371 3423
Fragments arranged by size:
3423 2371 997 117 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5500 5874 6020 387
Size: 1064 138 3911 374 146 1280
Fragments arranged by size:
3911 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2143 2605 2693 3378 3611 4092 4976
Size: 393 511 462 88 685 233 481 884
Cuts at: 4976 5573 6360 6628 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 884 787 685 597 511 481 462
393 268 233 88
EcoNI CCTnn'n_nnAGG
Cuts at: 2310 3020 2310
Size: 710 6203
EcoO109I rG'GnC_Cy
Cuts at: 2518 2592 3667 4076 2518
Size: 74 1075 409 5355
Fragments arranged by size:
5355 1075 409 74
EcoRI G'AATT_C
Cuts at: 4222 4600 4222
Size: 378 6535
FspI TGC'GCA
Cuts at: 5419 5419
Size: 6913
HaeII r_GCGC'y
Cuts at: 1678 6060 1678
Size: 4382 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2488 2520 2855 2857 2887
Size: 32 135 32 916 32 335 2 30
Cuts at: 2887 2889 5554 5586 5628 5660 1373
Size: 2 2665 32 42 32 2626
Fragments arranged by size:
2665 2626 916 335 135 42 32 32
32 32 32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2629 2995 3255 4257 4503 669
Size: 988 281 691 366 260 1002 246 3079
Fragments arranged by size:
3079 1002 988 691 366 281 260 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6913
HpaI GTT'AAC
Cuts at: 4503 4503
Size: 6913
KpnI G_GTAC'C
Cuts at: 4238 4238
Size: 6913
MfeI C'AATT_G
Cuts at: 4512 4512
Size: 6913
MluI A'CGCG_T
Cuts at: 6789 6789
Size: 6913
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2297 3408 6027 6211 1305
Size: 992 1111 2619 184 2007
Fragments arranged by size:
2619 2007 1111 992 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3020 10
Size: 54 1425 343 1188 3903
Fragments arranged by size:
3903 1425 1188 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2487 3488 4871 5230 5389 518
Size: 648 1321 1001 1383 359 159 2042
Fragments arranged by size:
2042 1383 1321 1001 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2351 2600 3332 4329 5006 6154 6399 1606
Size: 745 249 732 997 677 1148 245 2120
Fragments arranged by size:
2120 1148 997 745 732 677 249 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6361
NdeI CA'TA_TG
Cuts at: 387 3570 387
Size: 3183 3730
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6913
NotI GC'GGCC_GC
Cuts at: 4360 4360
Size: 6913
NsiI A_TGCA'T
Cuts at: 2952 3104 2952
Size: 152 6761
NspI r_CATG'y
Cuts at: 1132 1229 3102 4271 5816 1132
Size: 97 1873 1169 1545 2229
Fragments arranged by size:
2229 1873 1545 1169 97
PasI CC'CwG_GG
Cuts at: 1348 4159 1348
Size: 2811 4102
PciI A'CATG_T
Cuts at: 5812 5812
Size: 6913
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6913
PfoI T'CCnGG_A
Cuts at: 3120 3355 3120
Size: 235 6678
PmeI GTTT'AAAC
Cuts at: 4217 4217
Size: 6913
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4038 4070 4982 5014 6495 6527 4038
Size: 32 912 32 1481 32 4424
Fragments arranged by size:
4424 1481 912 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2518 2592 3667 4076 2518
Size: 74 1075 409 5355
Fragments arranged by size:
5355 1075 409 74
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6913
PsiI TTA'TAA
Cuts at: 3170 4483 3170
Size: 1313 5600
PstI C_TGCA'G
Cuts at: 830 4265 830
Size: 3435 3478
PvuI CG_AT'CG
Cuts at: 2001 3658 2001
Size: 1657 5256
PvuII CAG'CTG
Cuts at: 2600 3332 2600
Size: 732 6181
SalI G'TCGA_C
Cuts at: 1936 3253 4255 1936
Size: 1317 1002 4594
Fragments arranged by size:
4594 1317 1002
SapI GCTCTTCn'nnn_
Cuts at: 2350 2350
Size: 6913
SbfI CC_TGCA'GG
Cuts at: 4265 4265
Size: 6913
ScaI AGT'ACT
Cuts at: 5161 6873 5161
Size: 1712 5201
SexAI A'CCwGG_T
Cuts at: 2811 2811
Size: 6913
SfcI C'TryA_G
Cuts at: 826 1045 2086 2410 4261 5396 6077 6268 826
Size: 219 1041 324 1851 1135 681 191 1471
Fragments arranged by size:
1851 1471 1135 1041 681 324 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6913
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6913
SmaI CCC'GGG
Cuts at: 1872 4240 1872
Size: 2368 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2074 5017 5918 6180 820
Size: 197 926 131 2943 901 262 1553
Fragments arranged by size:
2943 1553 926 901 262 197 131
SnaBI TAC'GTA
Cuts at: 493 4704 493
Size: 4211 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6913
SphI G_CATG'C
Cuts at: 1132 4271 1132
Size: 3139 3774
SspI AAT'ATT
Cuts at: 5 52 4837 5
Size: 47 4785 2081
Fragments arranged by size:
4785 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6913
StyI C'CwwG_G
Cuts at: 513 1065 3021 3717 4352 513
Size: 552 1956 696 635 3074
Fragments arranged by size:
3074 1956 696 635 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4940 4957 5110 5295 5740 325
Size: 1255 3360 17 153 185 445 1498
Fragments arranged by size:
3360 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5159 6871 96
Size: 274 80 33 51 1443 3182 1712 138
Fragments arranged by size:
3182 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6873 6905 6873
Size: 32 6881
Tth111I GACn'n_nGTC
Cuts at: 1316 3290 1316
Size: 1974 4939
XbaI T'CTAG_A
Cuts at: 4249 4249
Size: 6913
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3715 3715
Size: 6913
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6913
XmaI C'CCGG_G
Cuts at: 1870 4238 1870
Size: 2368 4545
XmnI GAAnn'nnTTC
Cuts at: 2461 5042 2461
Size: 2581 4332
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4721 276
Size: 53 83 186 936 3187 2468
Fragments arranged by size:
3187 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI