Restriction map for
pFN21AE2068
(Circular) (Six-base) MAPSORT of: pFN21AE2068.seq Check: 9037 from: 1 to: 6886
pFN21AE2068 6886 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3020 3020
Size: 6886
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4696 278
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2533 4229 1937
Size: 596 1696 4594
Fragments arranged by size:
4594 1696 596
Acc65I G'GTAC_C
Cuts at: 4207 4207
Size: 6886
AclI AA'CG_TT
Cuts at: 1563 5013 5386 1563
Size: 3450 373 3063
Fragments arranged by size:
3450 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3069 3129 4949 6333 6601 1632
Size: 1437 60 1820 1384 268 1917
Fragments arranged by size:
1917 1820 1437 1384 268 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6689
AflIII A'CryG_T
Cuts at: 1203 5785 6762 1203
Size: 4582 977 1327
Fragments arranged by size:
4582 1327 977
AgeI A'CCGG_T
Cuts at: 5717 5717
Size: 6886
AhdI GACnn_n'nnGTC
Cuts at: 5615 5615
Size: 6886
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6886
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3161 4059 1184
Size: 1977 898 4011
Fragments arranged by size:
4011 1977 898
AlwNI CAG_nnn'CTG
Cuts at: 3134 3764 4629 6201 3134
Size: 630 865 1572 3819
Fragments arranged by size:
3819 1572 865 630
ApaLI G'TGCA_C
Cuts at: 4943 6099 4943
Size: 1156 5730
ApoI r'AATT_y
Cuts at: 1493 3354 4195 4421 4573 6682 1493
Size: 1861 841 226 152 2109 1697
Fragments arranged by size:
2109 1861 1697 841 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5440 160
Size: 872 4408 1606
Fragments arranged by size:
4408 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
AvaI C'yCGr_G
Cuts at: 1870 1943 4211 1870
Size: 73 2268 4545
Fragments arranged by size:
4545 2268 73
BamHI G'GATC_C
Cuts at: 4216 4583 4216
Size: 367 6519
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2690 3235 4154 4207 5662
Size: 325 217 627 903 545 919 53 1455
Cuts at: 5662 618
Size: 1842
Fragments arranged by size:
1842 1455 919 903 627 545 325 217
53
BanII G_rGCy'C
Cuts at: 1391 3736 3814 4017 4205 1391
Size: 2345 78 203 188 4072
Fragments arranged by size:
4072 2345 203 188 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2493 3109 3864 928
Size: 971 594 616 755 3950
Fragments arranged by size:
3950 971 755 616 594
BbvCI CC'TCA_GC
Cuts at: 4065 4065
Size: 6886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2168 2202 5077 5111 1903
Size: 34 231 34 2875 34 3678
Fragments arranged by size:
3678 2875 231 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2007 4778 5988 2007
Size: 2771 1210 2905
Fragments arranged by size:
2905 2771 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6886
BfrBI ATG'CAT
Cuts at: 2836 2836
Size: 6886
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5497 136
Size: 107 122 71 631 605 3825 1525
Fragments arranged by size:
3825 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6881 6881
Size: 6886
BlpI GC'TnA_GC
Cuts at: 3813 4303 3813
Size: 490 6396
Bme1580I G_kGCm'C
Cuts at: 1790 2695 4159 4947 6103 1790
Size: 905 1464 788 1156 2573
Fragments arranged by size:
2573 1464 1156 905 788
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6886
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4617 5575 442
Size: 416 53 83 399 24 3200 958 1753
Fragments arranged by size:
3200 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6886
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2878 2878
Size: 6886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2826 5546 1239
Size: 1587 2720 2579
Fragments arranged by size:
2720 2579 1587
Bpu10I CC'TnA_GC
Cuts at: 4065 4065
Size: 6886
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5011 5876 6174 5011
Size: 865 298 5723
Fragments arranged by size:
5723 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5549 882
Size: 617 4050 2219
Fragments arranged by size:
4050 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2831 4677 493
Size: 711 1627 1846 2702
Fragments arranged by size:
2702 1846 1627 711
BsaBI GATnn'nnATC
Cuts at: 6880 6880
Size: 6886
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4693 5075 5710 275
Size: 53 83 186 936 3160 382 635 1451
Fragments arranged by size:
3160 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5319 5717 5991 6138 6549 5319
Size: 398 274 147 411 5656
Fragments arranged by size:
5656 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6661
Fragments arranged by size:
6661 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6886
BseYI C'CCAG_C
Cuts at: 6089 6585 6089
Size: 496 6390
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3426 1163
Size: 573 1690 4623
Fragments arranged by size:
4623 1690 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2148 4336 5097 6125 1540
Size: 275 186 147 2188 761 1028 2301
Fragments arranged by size:
2301 2188 1028 761 275 186 147
BsiHKAI G_wGCw'C
Cuts at: 3814 3880 4017 4947 5032 6103 3814
Size: 66 137 930 85 1071 4597
Fragments arranged by size:
4597 1071 930 137 85 66
BsmI GAATG_Cn'
Cuts at: 4397 4490 4397
Size: 93 6793
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2695 3736 3814 3880 4017 4159 4205
Size: 399 905 1041 78 66 137 142 46
Cuts at: 4205 4947 5032 6103 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1041 905 742 399 142 137
85 78 66 46
BspEI T'CCGG_A
Cuts at: 6549 6549
Size: 6886
BspHI T'CATG_A
Cuts at: 2200 2520 4775 6505 2200
Size: 320 2255 1730 2581
Fragments arranged by size:
2581 2255 1730 320
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3020 4241 844
Size: 2176 1221 3489
Fragments arranged by size:
3489 2176 1221
BsrBI CCG'CTC
Cuts at: 1394 1823 4773 1394
Size: 429 2950 3507
Fragments arranged by size:
3507 2950 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3366 3489 5381 5555 65
Size: 1183 2118 123 1892 174 1396
Fragments arranged by size:
2118 1892 1396 1183 174 123
BsrFI r'CCGG_y
Cuts at: 1700 1810 5530 5717 1700
Size: 110 3720 187 2869
Fragments arranged by size:
3720 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6886
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6886
BssSI C'ACGA_G
Cuts at: 4946 5958 4946
Size: 1012 5874
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2235 3026 3473 794
Size: 1441 791 447 4207
Fragments arranged by size:
4207 1441 791 447
BstBI TT'CG_AA
Cuts at: 2068 3259 5690 2068
Size: 1191 2431 3264
Fragments arranged by size:
3264 2431 1191
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6886
BstYI r'GATC_y
Cuts at: 1972 2181 2859 4216 4583 4968 4985 6437 6523
Size: 209 678 1357 367 385 17 1452 86
Cuts at: 6523 6535 6881 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1357 678 385 367 346 209
86 17 12
BstZ17I GTA'TAC
Cuts at: 2534 2534
Size: 6886
Bsu36I CC'TnA_GG
Cuts at: 2262 2262
Size: 6886
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6334
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6147
Fragments arranged by size:
6147 683 56
BtsI GCAGTG_nn'
Cuts at: 3123 3837 4402 5196 5216 3123
Size: 714 565 794 20 4793
Fragments arranged by size:
4793 794 714 565 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6886
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6851
DraI TTT'AAA
Cuts at: 4190 4190
Size: 6886
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5893 809
Size: 507 4577 1802
Fragments arranged by size:
4577 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4125 4333 5222
Size: 54 1425 50 275 18 2295 208 889
Cuts at: 5222 6851 8
Size: 1629 43
Fragments arranged by size:
2295 1629 1425 889 275 208 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4333 1537
Size: 275 2521 4090
Fragments arranged by size:
4090 2521 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2847 3105 3183 3909 4816 1353
Size: 1494 258 78 726 907 3423
Fragments arranged by size:
3423 1494 907 726 258 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5473 5847 5993 387
Size: 1064 138 3884 374 146 1280
Fragments arranged by size:
3884 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3812 4015 3812
Size: 203 6683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2826 3069 3129 4949 5546 6333 6601
Size: 393 1194 243 60 1820 597 787 268
Cuts at: 6601 1239
Size: 1524
Fragments arranged by size:
1820 1524 1194 787 597 393 268 243
60
EcoO109I rG'GnC_Cy
Cuts at: 3397 3397
Size: 6886
EcoRI G'AATT_C
Cuts at: 4195 4573 4195
Size: 378 6508
EcoRV GAT'ATC
Cuts at: 2677 2677
Size: 6886
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3092 3311 3092
Size: 219 6667
FspI TGC'GCA
Cuts at: 5392 5392
Size: 6886
HaeII r_GCGC'y
Cuts at: 1678 6033 1678
Size: 4355 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2105 2137 2957 2989 3550
Size: 32 135 32 533 32 820 32 561
Cuts at: 3550 3582 3651 3683 4160 4192 5527 5559 5601
Size: 32 69 32 477 32 1335 32 42
Cuts at: 5601 5633 1373
Size: 32 2626
Fragments arranged by size:
2626 1335 820 561 533 477 135 69
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3796 4230 4476 669
Size: 988 281 1858 434 246 3079
Fragments arranged by size:
3079 1858 988 434 281 246
HindIII A'AGCT_T
Cuts at: 748 2157 748
Size: 1409 5477
HpaI GTT'AAC
Cuts at: 4476 4476
Size: 6886
KpnI G_GTAC'C
Cuts at: 4211 4211
Size: 6886
MfeI C'AATT_G
Cuts at: 3024 3174 4485 3024
Size: 150 1311 5425
Fragments arranged by size:
5425 1311 150
MluI A'CGCG_T
Cuts at: 6762 6762
Size: 6886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2282 2755 6000 6184 1305
Size: 977 473 3245 184 2007
Fragments arranged by size:
3245 2007 977 473 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4127 10
Size: 54 1425 343 2295 2769
Fragments arranged by size:
2769 2295 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3720 3723 4180 4844 5203 5362 518
Size: 648 2554 3 457 664 359 159 2042
Fragments arranged by size:
2554 2042 664 648 457 359 159 3
MspA1I CmG'CkG
Cuts at: 1606 3479 4302 4979 6127 6372 1606
Size: 1873 823 677 1148 245 2120
Fragments arranged by size:
2120 1873 1148 823 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6334
NdeI CA'TA_TG
Cuts at: 387 3328 3790 3910 4181 387
Size: 2941 462 120 271 3092
Fragments arranged by size:
3092 2941 462 271 120
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6886
NotI GC'GGCC_GC
Cuts at: 4333 4333
Size: 6886
NsiI A_TGCA'T
Cuts at: 2838 2838
Size: 6886
NspI r_CATG'y
Cuts at: 1132 1229 2179 4244 5789 1132
Size: 97 950 2065 1545 2229
Fragments arranged by size:
2229 2065 1545 950 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6886
PciI A'CATG_T
Cuts at: 5785 5785
Size: 6886
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6886
PmeI GTTT'AAAC
Cuts at: 4190 4190
Size: 6886
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4955 4987 6468 6500 4955
Size: 32 1481 32 5341
Fragments arranged by size:
5341 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3397 3397
Size: 6886
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6886
PsiI TTA'TAA
Cuts at: 3872 4456 3872
Size: 584 6302
PstI C_TGCA'G
Cuts at: 830 2231 2424 3130 4238 830
Size: 1401 193 706 1108 3478
Fragments arranged by size:
3478 1401 1108 706 193
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6886
PvuII CAG'CTG
Cuts at: 3479 3479
Size: 6886
SacI G_AGCT'C
Cuts at: 3814 4017 3814
Size: 203 6683
SalI G'TCGA_C
Cuts at: 1936 4228 1936
Size: 2292 4594
SbfI CC_TGCA'GG
Cuts at: 4238 4238
Size: 6886
ScaI AGT'ACT
Cuts at: 5134 6846 5134
Size: 1712 5174
SfcI C'TryA_G
Cuts at: 826 1045 2227 2420 2685 3126 4234 5369 6050
Size: 219 1182 193 265 441 1108 1135 681
Cuts at: 6050 6241 826
Size: 191 1471
Fragments arranged by size:
1471 1182 1135 1108 681 441 265 219
193 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6886
SmaI CCC'GGG
Cuts at: 1872 4213 1872
Size: 2341 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4990 5891 6153 820
Size: 197 926 3047 901 262 1553
Fragments arranged by size:
3047 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4677 493
Size: 4184 2702
SpeI A'CTAG_T
Cuts at: 152 3512 152
Size: 3360 3526
SphI G_CATG'C
Cuts at: 1132 2179 4244 1132
Size: 1047 2065 3774
Fragments arranged by size:
3774 2065 1047
SspI AAT'ATT
Cuts at: 5 52 2785 4810 5
Size: 47 2733 2025 2081
Fragments arranged by size:
2733 2081 2025 47
StuI AGG'CCT
Cuts at: 1517 2030 2040 1517
Size: 513 10 6363
Fragments arranged by size:
6363 513 10
StyI C'CwwG_G
Cuts at: 513 1065 2729 3586 3630 4325 513
Size: 552 1664 857 44 695 3074
Fragments arranged by size:
3074 1664 857 695 552 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2391 4913 4930 5083 5268 5713 325
Size: 1255 811 2522 17 153 185 445 1498
Fragments arranged by size:
2522 1498 1255 811 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2540 5132 6844
Size: 274 80 33 51 1443 563 2592 1712
Cuts at: 6844 96
Size: 138
Fragments arranged by size:
2592 1712 1443 563 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3499 3531 6846 6878 3499
Size: 32 3315 32 3507
Fragments arranged by size:
3507 3315 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6886
XbaI T'CTAG_A
Cuts at: 4222 4222
Size: 6886
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2761 3455 2761
Size: 694 6192
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6886
XmaI C'CCGG_G
Cuts at: 1870 4211 1870
Size: 2341 4545
XmnI GAAnn'nnTTC
Cuts at: 3612 5015 3612
Size: 1403 5483
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4694 276
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstXI DraIII EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI