Restriction map for
pFN21AE2064
(Circular) (Six-base) MAPSORT of: pFN21AE2064.seq Check: 2974 from: 1 to: 6841
pFN21AE2064 6841 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4651 278
Size: 53 83 186 936 3115 2468
Fragments arranged by size:
3115 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4184 1937
Size: 2247 4594
Acc65I G'GTAC_C
Cuts at: 3903 4162 3903
Size: 259 6582
AclI AA'CG_TT
Cuts at: 1563 4968 5341 1563
Size: 3405 373 3063
Fragments arranged by size:
3405 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3104 4113 4904 6288 6556 1632
Size: 1472 1009 791 1384 268 1917
Fragments arranged by size:
1917 1472 1384 1009 791 268
AfeI AGC'GCT
Cuts at: 2138 2138
Size: 6841
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6644
AflIII A'CryG_T
Cuts at: 1203 3504 5740 6717 1203
Size: 2301 2236 977 1327
Fragments arranged by size:
2301 2236 1327 977
AgeI A'CCGG_T
Cuts at: 5672 5672
Size: 6841
AhdI GACnn_n'nnGTC
Cuts at: 2296 5570 2296
Size: 3274 3567
AleI CACnn'nnGTG
Cuts at: 1166 3206 1166
Size: 2040 4801
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2056 3439 1184
Size: 872 1383 4586
Fragments arranged by size:
4586 1383 872
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2762 2794 2762
Size: 32 6809
AlwNI CAG_nnn'CTG
Cuts at: 2479 2617 2921 3322 3460 3625 3871 4109 4584
Size: 138 304 401 138 165 246 238 475
Cuts at: 4584 6156 2479
Size: 1572 3164
Fragments arranged by size:
3164 1572 475 401 304 246 238 165
138 138
ApaI G_GGCC'C
Cuts at: 2287 2287
Size: 6841
ApaLI G'TGCA_C
Cuts at: 3629 4898 6054 3629
Size: 1269 1156 4416
Fragments arranged by size:
4416 1269 1156
ApoI r'AATT_y
Cuts at: 1493 4150 4376 4528 6637 1493
Size: 2657 226 152 2109 1697
Fragments arranged by size:
2657 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5395 160
Size: 872 4363 1606
Fragments arranged by size:
4363 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6841
AvaI C'yCGr_G
Cuts at: 1870 1943 3230 3947 4166 1870
Size: 73 1287 717 219 4545
Fragments arranged by size:
4545 1287 717 219 73
AvrII C'CTAG_G
Cuts at: 2536 2536
Size: 6841
BamHI G'GATC_C
Cuts at: 2149 2721 4171 4538 2149
Size: 572 1450 367 4452
Fragments arranged by size:
4452 1450 572 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2115 2649 3903 4162 5617
Size: 325 217 627 328 534 1254 259 1455
Cuts at: 5617 618
Size: 1842
Fragments arranged by size:
1842 1455 1254 627 534 328 325 259
217
BanII G_rGCy'C
Cuts at: 1391 2089 2192 2205 2268 2287 2892 4160 1391
Size: 698 103 13 63 19 605 1268 4072
Fragments arranged by size:
4072 1268 698 605 103 63 19 13
BbeI G_GCGC'C
Cuts at: 2119 2119
Size: 6841
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2985 3092 928
Size: 971 1086 107 4677
Fragments arranged by size:
4677 1086 971 107
BbvCI CC'TCA_GC
Cuts at: 2844 2914 3443 2844
Size: 70 529 6242
Fragments arranged by size:
6242 529 70
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5032 5066 1903
Size: 34 3095 34 3678
Fragments arranged by size:
3678 3095 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4733 5943 4733
Size: 1210 5631
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6841
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3158 3191 3233
Size: 107 122 71 631 605 1486 33 42
Cuts at: 3233 4055 5452 136
Size: 822 1397 1525
Fragments arranged by size:
1525 1486 1397 822 631 605 122 107
71 42 33
BglII A'GATC_T
Cuts at: 6836 6836
Size: 6841
BlpI GC'TnA_GC
Cuts at: 4258 4258
Size: 6841
Bme1580I G_kGCm'C
Cuts at: 1790 2287 3633 4902 6058 1790
Size: 497 1346 1269 1156 2573
Fragments arranged by size:
2573 1346 1269 1156 497
BmgBI CAC'GTC
Cuts at: 1318 3190 3292 1318
Size: 1872 102 4867
Fragments arranged by size:
4867 1872 102
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3190 4572 5530
Size: 416 53 83 399 24 1773 1382 958
Cuts at: 5530 442
Size: 1753
Fragments arranged by size:
1773 1753 1382 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6841
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2076 2719 2806 2076
Size: 643 87 6111
Fragments arranged by size:
6111 643 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2316 3395 3905 4010 5501 1239
Size: 1077 1079 510 105 1491 2579
Fragments arranged by size:
2579 1491 1079 1077 510 105
Bpu10I CC'TnA_GC
Cuts at: 2186 2204 2844 2914 3443 2186
Size: 18 640 70 529 5584
Fragments arranged by size:
5584 640 529 70 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3521 4966 5831 6129 3521
Size: 1445 865 298 4233
Fragments arranged by size:
4233 1445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3313 3545 3714 5504 882
Size: 617 1814 232 169 1790 2219
Fragments arranged by size:
2219 1814 1790 617 232 169
BsaAI yAC'GTr
Cuts at: 493 1204 4632 493
Size: 711 3428 2702
Fragments arranged by size:
3428 2702 711
BsaBI GATnn'nnATC
Cuts at: 6835 6835
Size: 6841
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2116 2462 3363 4648
Size: 53 83 186 936 583 346 901 1285
Cuts at: 4648 5030 5665 275
Size: 382 635 1451
Fragments arranged by size:
1451 1285 936 901 635 583 382 346
186 83 53
BsaWI w'CCGG_w
Cuts at: 2152 5274 5672 5946 6093 6504 2152
Size: 3122 398 274 147 411 2489
Fragments arranged by size:
3122 2489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6616
Fragments arranged by size:
6616 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2293 2410 2861 2902 2969 3572 4007 4028
Size: 1107 117 451 41 67 603 435 21
Cuts at: 4028 4040 1186
Size: 12 3987
Fragments arranged by size:
3987 1107 603 451 435 117 67 41
21 12
BseYI C'CCAG_C
Cuts at: 3100 6044 6540 3100
Size: 2944 496 3401
Fragments arranged by size:
3401 2944 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6268
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2068 2198 3604 4291 5052 6080
Size: 275 186 67 130 1406 687 761 1028
Cuts at: 6080 1540
Size: 2301
Fragments arranged by size:
2301 1406 1028 761 687 275 186 130
67
BsiHKAI G_wGCw'C
Cuts at: 2205 3633 4902 4987 6058 2205
Size: 1428 1269 85 1071 2988
Fragments arranged by size:
2988 1428 1269 1071 85
BsmI GAATG_Cn'
Cuts at: 4352 4445 4352
Size: 93 6748
BsmBI CGTCTCn'nnnn_
Cuts at: 2621 2660 2621
Size: 39 6802
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2089 2192 2205 2268 2287 2892 3633
Size: 399 299 103 13 63 19 605 741
Cuts at: 3633 4160 4902 4987 6058 1391
Size: 527 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 741 605 527 399 299
103 85 63 19 13
BspEI T'CCGG_A
Cuts at: 2152 6504 2152
Size: 4352 2489
BspHI T'CATG_A
Cuts at: 4730 6460 4730
Size: 1730 5111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2037 3087 3132 3879 4196 844
Size: 1193 1050 45 747 317 3489
Fragments arranged by size:
3489 1193 1050 747 317 45
BsrBI CCG'CTC
Cuts at: 1394 1823 2373 2763 3073 3952 4728 1394
Size: 429 550 390 310 879 776 3507
Fragments arranged by size:
3507 879 776 550 429 390 310
BsrDI GCAATG_nn'
Cuts at: 65 1248 2537 3813 5336 5510 65
Size: 1183 1289 1276 1523 174 1396
Fragments arranged by size:
1523 1396 1289 1276 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3033 4097 5485 5672 1700
Size: 110 1223 1064 1388 187 2869
Fragments arranged by size:
2869 1388 1223 1064 187 110
BsrGI T'GTAC_A
Cuts at: 96 3354 96
Size: 3258 3583
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6841
BssSI C'ACGA_G
Cuts at: 2269 4901 5913 2269
Size: 2632 1012 3197
Fragments arranged by size:
3197 2632 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3211 794
Size: 2417 4424
BstBI TT'CG_AA
Cuts at: 5645 5645
Size: 6841
BstEII G'GTnAC_C
Cuts at: 1184 2836 1184
Size: 1652 5189
BstYI r'GATC_y
Cuts at: 1972 2149 2721 3096 3915 4171 4538 4923 4940
Size: 177 572 375 819 256 367 385 17
Cuts at: 4940 6392 6478 6490 6836 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 819 572 385 375 367 346
256 177 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2163 2192 3543 513
Size: 552 939 159 29 1351 3811
Fragments arranged by size:
3811 1351 939 552 159 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6102
Fragments arranged by size:
6102 683 56
BtsI GCAGTG_nn'
Cuts at: 4357 5151 5171 4357
Size: 794 20 6027
Fragments arranged by size:
6027 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6841
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6806
DraI TTT'AAA
Cuts at: 4145 4145
Size: 6841
DraIII CAC_nnn'GTG
Cuts at: 2689 3292 2689
Size: 603 6238
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2746 5848 809
Size: 507 1430 3102 1802
Fragments arranged by size:
3102 1802 1430 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2065 2195 2427
Size: 54 1425 50 275 18 235 130 232
Cuts at: 2427 2481 2964 3114 3495 3528 3699 4288 5177
Size: 54 483 150 381 33 171 589 889
Cuts at: 5177 6806 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 589 483 381 275 235
232 171 150 130 54 54 50 43
33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2065 2195 4288 1537
Size: 275 253 130 2093 4090
Fragments arranged by size:
4090 2093 275 253 130
EarI CTCTTCn'nnn_
Cuts at: 1353 3999 4017 4029 4771 1353
Size: 2646 18 12 742 3423
Fragments arranged by size:
3423 2646 742 18 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3032 5428 5802 5948 387
Size: 1064 138 1443 2396 374 146 1280
Fragments arranged by size:
2396 1443 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2203 2203
Size: 6841
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2316 3104 3395 3905 4010 4113 4904
Size: 393 684 788 291 510 105 103 791
Cuts at: 4904 5501 6288 6556 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 791 788 787 684 597 510 393
291 268 105 103
EcoNI CCTnn'n_nnAGG
Cuts at: 2239 2239
Size: 6841
EcoO109I rG'GnC_Cy
Cuts at: 2182 2283 2284 2476 2555 2851 3076 3868 2182
Size: 101 1 192 79 296 225 792 5155
Fragments arranged by size:
5155 792 296 225 192 101 79 1
EcoRI G'AATT_C
Cuts at: 4150 4528 4150
Size: 378 6463
EcoRV GAT'ATC
Cuts at: 3430 3430
Size: 6841
FspI TGC'GCA
Cuts at: 3470 5347 3470
Size: 1877 4964
HaeII r_GCGC'y
Cuts at: 1678 2119 2140 2248 3282 3350 3450 5988 1678
Size: 441 21 108 1034 68 100 2538 2531
Fragments arranged by size:
2538 2531 1034 441 108 100 68 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2282 2314 3719 3751 5482
Size: 32 135 32 710 32 1405 32 1731
Cuts at: 5482 5514 5556 5588 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1731 1405 710 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2739 4185 4431 669
Size: 988 281 801 1446 246 3079
Fragments arranged by size:
3079 1446 988 801 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6841
HpaI GTT'AAC
Cuts at: 4431 4431
Size: 6841
KasI G'GCGC_C
Cuts at: 2115 2115
Size: 6841
KpnI G_GTAC'C
Cuts at: 3907 4166 3907
Size: 259 6582
MfeI C'AATT_G
Cuts at: 4440 4440
Size: 6841
MluI A'CGCG_T
Cuts at: 6717 6717
Size: 6841
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2155 2542 2643 2730 3336 3624 5955 6139
Size: 850 387 101 87 606 288 2331 184
Cuts at: 6139 1305
Size: 2007
Fragments arranged by size:
2331 2007 850 606 387 288 184 101
87
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2429 2483 2966 3116 3497
Size: 54 1425 343 597 54 483 150 381
Cuts at: 3497 10
Size: 3354
Fragments arranged by size:
3354 1425 597 483 381 343 150 54
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2456 3153 3206 3485 3503 3858 4799
Size: 648 1290 697 53 279 18 355 941
Cuts at: 4799 5158 5317 518
Size: 359 159 2042
Fragments arranged by size:
2042 1290 941 697 648 359 355 279
159 53 18
MspA1I CmG'CkG
Cuts at: 1606 2165 2194 2350 2843 3664 4257 4934 6082
Size: 559 29 156 493 821 593 677 1148
Cuts at: 6082 6327 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 821 677 593 559 493 245
156 29
NaeI GCC'GGC
Cuts at: 4099 4099
Size: 6841
NarI GG'CG_CC
Cuts at: 2116 2116
Size: 6841
NcoI C'CATG_G
Cuts at: 513 1065 2004 3543 513
Size: 552 939 1539 3811
Fragments arranged by size:
3811 1539 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6841
NgoMIV G'CCGG_C
Cuts at: 4097 4097
Size: 6841
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6841
NotI GC'GGCC_GC
Cuts at: 2065 4288 2065
Size: 2223 4618
NspI r_CATG'y
Cuts at: 1132 1229 3182 3209 3508 4199 5744 1132
Size: 97 1953 27 299 691 1545 2229
Fragments arranged by size:
2229 1953 1545 691 299 97 27
PasI CC'CwG_GG
Cuts at: 1348 4109 1348
Size: 2761 4080
PciI A'CATG_T
Cuts at: 3504 5740 3504
Size: 2236 4605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2479 2933 2991 3292 3388 4109 1108
Size: 1371 454 58 301 96 721 3840
Fragments arranged by size:
3840 1371 721 454 301 96 58
PfoI T'CCnGG_A
Cuts at: 2765 2792 2802 2867 3585 2765
Size: 27 10 65 718 6021
Fragments arranged by size:
6021 718 65 27 10
PmeI GTTT'AAAC
Cuts at: 4145 4145
Size: 6841
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3224 3256 4910 4942 6423 6455 3224
Size: 32 1654 32 1481 32 3610
Fragments arranged by size:
3610 1654 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2182 2476 2555 3076 3868 2182
Size: 294 79 521 792 5155
Fragments arranged by size:
5155 792 521 294 79
PshAI GACnn'nnGTC
Cuts at: 1613 3750 1613
Size: 2137 4704
PsiI TTA'TAA
Cuts at: 4411 4411
Size: 6841
PspOMI G'GGCC_C
Cuts at: 2283 2283
Size: 6841
PspXI vC'TCGA_Gb
Cuts at: 3230 3230
Size: 6841
PstI C_TGCA'G
Cuts at: 830 2243 2599 2676 3414 4193 830
Size: 1413 356 77 738 779 3478
Fragments arranged by size:
3478 1413 779 738 356 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6841
RsrII CG'GwC_CG
Cuts at: 2493 3688 2493
Size: 1195 5646
SacI G_AGCT'C
Cuts at: 2205 2205
Size: 6841
SacII CC_GC'GG
Cuts at: 2166 2195 2166
Size: 29 6812
SalI G'TCGA_C
Cuts at: 1936 4183 1936
Size: 2247 4594
SanDI GG'GwC_CC
Cuts at: 2555 2555
Size: 6841
SbfI CC_TGCA'GG
Cuts at: 2243 4193 2243
Size: 1950 4891
ScaI AGT'ACT
Cuts at: 5089 6801 5089
Size: 1712 5129
SexAI A'CCwGG_T
Cuts at: 2772 3624 3846 2772
Size: 852 222 5767
Fragments arranged by size:
5767 852 222
SfcI C'TryA_G
Cuts at: 826 1045 2239 2595 2672 3410 4189 5324 6005
Size: 219 1194 356 77 738 779 1135 681
Cuts at: 6005 6196 826
Size: 191 1471
Fragments arranged by size:
1471 1194 1135 779 738 681 356 219
191 77
SfoI GGC'GCC
Cuts at: 2117 2117
Size: 6841
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6841
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6841
SmaI CCC'GGG
Cuts at: 1872 4168 1872
Size: 2296 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3230 3536 4945 5846 6108 820
Size: 197 926 1287 306 1409 901 262 1553
Fragments arranged by size:
1553 1409 1287 926 901 306 262 197
SnaBI TAC'GTA
Cuts at: 493 4632 493
Size: 4139 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6841
SphI G_CATG'C
Cuts at: 1132 4199 1132
Size: 3067 3774
SspI AAT'ATT
Cuts at: 5 52 4765 5
Size: 47 4713 2081
Fragments arranged by size:
4713 2081 47
StuI AGG'CCT
Cuts at: 1517 4058 1517
Size: 2541 4300
StyI C'CwwG_G
Cuts at: 513 1065 2004 2400 2536 2832 2967 3543 4059
Size: 552 939 396 136 296 135 576 516
Cuts at: 4059 4280 513
Size: 221 3074
Fragments arranged by size:
3074 939 576 552 516 396 296 221
136 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2831 3618 3837 3870 4868 4885 5038
Size: 1255 1251 787 219 33 998 17 153
Cuts at: 5038 5223 5668 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1251 998 787 445 219 185
153 33 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2784 3354 5087
Size: 274 80 33 51 1443 807 570 1733
Cuts at: 5087 6799 96
Size: 1712 138
Fragments arranged by size:
1733 1712 1443 807 570 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2988 3020 6801 6833 2988
Size: 32 3781 32 2996
Fragments arranged by size:
3781 2996 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3462 3848 1316
Size: 2146 386 4309
Fragments arranged by size:
4309 2146 386
XbaI T'CTAG_A
Cuts at: 4177 4177
Size: 6841
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2308 3289 3340 4057 2011
Size: 297 981 51 717 4795
Fragments arranged by size:
4795 981 717 297 51
XhoI C'TCGA_G
Cuts at: 1943 3230 1943
Size: 1287 5554
XmaI C'CCGG_G
Cuts at: 1870 4166 1870
Size: 2296 4545
XmnI GAAnn'nnTTC
Cuts at: 2569 3089 4970 2569
Size: 520 1881 4440
Fragments arranged by size:
4440 1881 520
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4649 276
Size: 53 83 186 936 3115 2468
Fragments arranged by size:
3115 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I
FalI FseI FspAI NruI NsiI PacI PmlI PsrI
PvuII SapI SfiI SrfI SwaI