Restriction map for
pFN21AE2062
(Circular) (Six-base) MAPSORT of: pFN21AE2062.seq Check: 6057 from: 1 to: 7168
pFN21AE2062 7168 bp
With 182 enzymes: SgfI *
November 16, 2010 14:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4978 278
Size: 53 83 186 936 3442 2468
Fragments arranged by size:
3442 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2343 4041 4511 1937
Size: 406 1698 470 4594
Fragments arranged by size:
4594 1698 470 406
Acc65I G'GTAC_C
Cuts at: 4489 4489
Size: 7168
AclI AA'CG_TT
Cuts at: 1563 4250 5295 5668 1563
Size: 2687 1045 373 3063
Fragments arranged by size:
3063 2687 1045 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2062 3214 4172 4362 5231 6615 6883 1632
Size: 430 1152 958 190 869 1384 268 1917
Fragments arranged by size:
1917 1384 1152 958 869 430 268 190
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6971
AflIII A'CryG_T
Cuts at: 1203 2730 6067 7044 1203
Size: 1527 3337 977 1327
Fragments arranged by size:
3337 1527 1327 977
AgeI A'CCGG_T
Cuts at: 5999 5999
Size: 7168
AhdI GACnn_n'nnGTC
Cuts at: 3814 5897 3814
Size: 2083 5085
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7168
AlwNI CAG_nnn'CTG
Cuts at: 3092 4911 6483 3092
Size: 1819 1572 3777
Fragments arranged by size:
3777 1819 1572
ApaI G_GGCC'C
Cuts at: 2247 2913 2247
Size: 666 6502
ApaLI G'TGCA_C
Cuts at: 2234 3248 5225 6381 2234
Size: 1014 1977 1156 3021
Fragments arranged by size:
3021 1977 1156 1014
ApoI r'AATT_y
Cuts at: 1493 4281 4477 4703 4855 6964 1493
Size: 2788 196 226 152 2109 1697
Fragments arranged by size:
2788 2109 1697 226 196 152
AseI AT'TA_AT
Cuts at: 160 1032 5722 160
Size: 872 4690 1606
Fragments arranged by size:
4690 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7168
AvaI C'yCGr_G
Cuts at: 1870 1943 3749 4493 1870
Size: 73 1806 744 4545
Fragments arranged by size:
4545 1806 744 73
BamHI G'GATC_C
Cuts at: 4498 4865 4498
Size: 367 6801
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2291 4489 5944 618
Size: 325 217 627 504 2198 1455 1842
Fragments arranged by size:
2198 1842 1455 627 504 325 217
BanII G_rGCy'C
Cuts at: 1391 2247 2913 3739 4109 4487 1391
Size: 856 666 826 370 378 4072
Fragments arranged by size:
4072 856 826 666 378 370
BbeI G_GCGC'C
Cuts at: 2295 2295
Size: 7168
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3151 4275 928
Size: 971 1252 1124 3821
Fragments arranged by size:
3821 1252 1124 971
BbvCI CC'TCA_GC
Cuts at: 4319 4319
Size: 7168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2591 2625 5359 5393 1903
Size: 34 654 34 2734 34 3678
Fragments arranged by size:
3678 2734 654 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2086 3278 4449 5060 6270 2086
Size: 1192 1171 611 1210 2984
Fragments arranged by size:
2984 1210 1192 1171 611
BclI T'GATC_A
Cuts at: 1548 2211 1548
Size: 663 6505
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2767 2917 5779
Size: 107 122 71 631 605 1095 150 2862
Cuts at: 5779 136
Size: 1525
Fragments arranged by size:
2862 1525 1095 631 605 150 122 107
71
BglII A'GATC_T
Cuts at: 4089 7163 4089
Size: 3074 4094
BlpI GC'TnA_GC
Cuts at: 4585 4585
Size: 7168
Bme1580I G_kGCm'C
Cuts at: 1790 2238 2247 2913 3252 4236 5229 6385 1790
Size: 448 9 666 339 984 993 1156 2573
Fragments arranged by size:
2573 1156 993 984 666 448 339 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7168
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3461 4899 5857
Size: 416 53 83 399 24 2044 1438 958
Cuts at: 5857 442
Size: 1753
Fragments arranged by size:
2044 1753 1438 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7168
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3325 3721 3746 3325
Size: 396 25 6747
Fragments arranged by size:
6747 396 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2665 3152 4002 4386 5828 1239
Size: 1426 487 850 384 1442 2579
Fragments arranged by size:
2579 1442 1426 850 487 384
Bpu10I CC'TnA_GC
Cuts at: 4319 4319
Size: 7168
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2915 2924 5293 6158 6456 2915
Size: 9 2369 865 298 3627
Fragments arranged by size:
3627 2369 865 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3397 4237 5831 882
Size: 617 1898 840 1594 2219
Fragments arranged by size:
2219 1898 1594 840 617
BsaAI yAC'GTr
Cuts at: 493 1204 2155 2733 2884 3027 4959 493
Size: 711 951 578 151 143 1932 2702
Fragments arranged by size:
2702 1932 951 711 578 151 143
BsaBI GATnn'nnATC
Cuts at: 7162 7162
Size: 7168
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2292 4975 5357 5992
Size: 53 83 186 936 759 2683 382 635
Cuts at: 5992 275
Size: 1451
Fragments arranged by size:
2683 1451 936 759 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2393 5601 5999 6273 6420 6831 2393
Size: 3208 398 274 147 411 2730
Fragments arranged by size:
3208 2730 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2808 2838 1178
Size: 30 165 30 1405 30 5508
Fragments arranged by size:
5508 1405 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2832 3301 3803 1186
Size: 1646 469 502 4551
Fragments arranged by size:
4551 1646 502 469
BseYI C'CCAG_C
Cuts at: 2013 2542 2856 3014 6371 6867 2013
Size: 529 314 158 3357 496 2314
Fragments arranged by size:
3357 2314 529 496 314 158
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3649 4171 1163
Size: 573 1913 522 4160
Fragments arranged by size:
4160 1913 573 522
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2759 4618 5379 6407 1540
Size: 275 186 758 1859 761 1028 2301
Fragments arranged by size:
2301 1859 1028 761 758 275 186
BsiHKAI G_wGCw'C
Cuts at: 2238 2464 3252 5229 5314 6385 2238
Size: 226 788 1977 85 1071 3021
Fragments arranged by size:
3021 1977 1071 788 226 85
BsmI GAATG_Cn'
Cuts at: 4679 4772 4679
Size: 93 7075
BsmBI CGTCTCn'nnnn_
Cuts at: 2814 2814
Size: 7168
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2238 2247 2464 2913 3252 3739 4109
Size: 399 448 9 217 449 339 487 370
Cuts at: 4109 4236 4487 5229 5314 6385 1391
Size: 127 251 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 487 449 448 399 370
339 251 217 127 85 9
BspEI T'CCGG_A
Cuts at: 2393 6831 2393
Size: 4438 2730
BspHI T'CATG_A
Cuts at: 5057 6787 5057
Size: 1730 5438
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4523 844
Size: 3679 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5055 1394
Size: 429 3232 3507
Fragments arranged by size:
3507 3232 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5663 5837 65
Size: 1183 4415 174 1396
Fragments arranged by size:
4415 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2766 2791 2948 3241 5812 5999 1700
Size: 110 956 25 157 293 2571 187 2869
Fragments arranged by size:
2869 2571 956 293 187 157 110 25
BsrGI T'GTAC_A
Cuts at: 96 4200 96
Size: 4104 3064
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7168
BssSI C'ACGA_G
Cuts at: 5228 6240 5228
Size: 1012 6156
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4231 794
Size: 3437 3731
BstBI TT'CG_AA
Cuts at: 5972 5972
Size: 7168
BstEII G'GTnAC_C
Cuts at: 1184 2041 3538 1184
Size: 857 1497 4814
Fragments arranged by size:
4814 1497 857
BstXI CCAn_nnnn'nTGG
Cuts at: 3844 4008 3844
Size: 164 7004
BstYI r'GATC_y
Cuts at: 1972 4089 4498 4865 5250 5267 6719 6805 6817
Size: 2117 409 367 385 17 1452 86 12
Cuts at: 6817 7163 1972
Size: 346 1977
Fragments arranged by size:
2117 1977 1452 409 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 4042 4042
Size: 7168
Bsu36I CC'TnA_GG
Cuts at: 3786 3786
Size: 7168
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 2961 3543 4128 513
Size: 552 939 42 915 582 585 3553
Fragments arranged by size:
3553 939 915 585 582 552 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2594 487
Size: 683 56 1368 5061
Fragments arranged by size:
5061 1368 683 56
BtsI GCAGTG_nn'
Cuts at: 3510 4236 4462 4684 5478 5498 3510
Size: 726 226 222 794 20 5180
Fragments arranged by size:
5180 794 726 226 222 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7133
DraI TTT'AAA
Cuts at: 4472 4472
Size: 7168
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6175 809
Size: 507 4859 1802
Fragments arranged by size:
4859 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2517 3546 3834
Size: 54 1425 50 275 18 687 1029 288
Cuts at: 3834 4615 5504 7133 8
Size: 781 889 1629 43
Fragments arranged by size:
1629 1425 1029 889 781 687 288 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4615 1537
Size: 275 2803 4090
Fragments arranged by size:
4090 2803 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2106 3091 3364 5098 1353
Size: 753 985 273 1734 3423
Fragments arranged by size:
3423 1734 985 753 273
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3512 5755 6129 6275 387
Size: 1064 138 1923 2243 374 146 1280
Fragments arranged by size:
2243 1923 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2665 3152 3214 4002 4172 4362
Size: 393 430 603 487 62 788 170 190
Cuts at: 4362 4386 5231 5828 6615 6883 1239
Size: 24 845 597 787 268 1524
Fragments arranged by size:
1524 845 788 787 603 597 487 430
393 268 190 170 62 24
EcoO109I rG'GnC_Cy
Cuts at: 2243 3415 2243
Size: 1172 5996
EcoRI G'AATT_C
Cuts at: 4281 4477 4855 4281
Size: 196 378 6594
Fragments arranged by size:
6594 378 196
EcoRV GAT'ATC
Cuts at: 3054 3955 3054
Size: 901 6267
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3244 4386 3244
Size: 1142 6026
FspI TGC'GCA
Cuts at: 5674 5674
Size: 7168
HaeII r_GCGC'y
Cuts at: 1678 2295 3092 6315 1678
Size: 617 797 3223 2531
Fragments arranged by size:
3223 2531 797 617
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2372 2404 3584 3616 3800
Size: 32 135 32 800 32 1180 32 184
Cuts at: 3800 3832 4049 4081 5809 5841 5883 5915 1373
Size: 32 217 32 1728 32 42 32 2626
Fragments arranged by size:
2626 1728 1180 800 217 184 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2956 3075 4512 4758 669
Size: 988 281 1018 119 1437 246 3079
Fragments arranged by size:
3079 1437 1018 988 281 246 119
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7168
HpaI GTT'AAC
Cuts at: 2956 4758 2956
Size: 1802 5366
KasI G'GCGC_C
Cuts at: 2291 2291
Size: 7168
KpnI G_GTAC'C
Cuts at: 4493 4493
Size: 7168
MfeI C'AATT_G
Cuts at: 4767 4767
Size: 7168
MluI A'CGCG_T
Cuts at: 7044 7044
Size: 7168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2198 2794 3348 6282 6466 1305
Size: 893 596 554 2934 184 2007
Fragments arranged by size:
2934 2007 893 596 554 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3836 10
Size: 54 1425 343 2004 3342
Fragments arranged by size:
3342 2004 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2742 3377 4053 4208 5126 5485 5644
Size: 648 1576 635 676 155 918 359 159
Cuts at: 5644 518
Size: 2042
Fragments arranged by size:
2042 1576 918 676 648 635 359 159
155
MspA1I CmG'CkG
Cuts at: 1606 2013 3334 3832 4584 5261 6409 6654 1606
Size: 407 1321 498 752 677 1148 245 2120
Fragments arranged by size:
2120 1321 1148 752 677 498 407 245
NaeI GCC'GGC
Cuts at: 2793 3243 2793
Size: 450 6718
NarI GG'CG_CC
Cuts at: 2292 2292
Size: 7168
NcoI C'CATG_G
Cuts at: 513 1065 2004 2046 513
Size: 552 939 42 5635
Fragments arranged by size:
5635 939 552 42
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7168
NgoMIV G'CCGG_C
Cuts at: 2791 3241 2791
Size: 450 6718
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7168
NotI GC'GGCC_GC
Cuts at: 4615 4615
Size: 7168
NspI r_CATG'y
Cuts at: 1132 1229 4229 4526 6071 1132
Size: 97 3000 297 1545 2229
Fragments arranged by size:
3000 2229 1545 297 97
PasI CC'CwG_GG
Cuts at: 1348 3463 4118 1348
Size: 2115 655 4398
Fragments arranged by size:
4398 2115 655
PciI A'CATG_T
Cuts at: 6067 6067
Size: 7168
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2574 3092 1108
Size: 1466 518 5184
Fragments arranged by size:
5184 1466 518
PmeI GTTT'AAAC
Cuts at: 4472 4472
Size: 7168
PmlI CAC'GTG
Cuts at: 2884 2884
Size: 7168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5237 5269 6750 6782 5237
Size: 32 1481 32 5623
Fragments arranged by size:
5623 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3415 3415
Size: 7168
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7168
PsiI TTA'TAA
Cuts at: 4738 4738
Size: 7168
PspOMI G'GGCC_C
Cuts at: 2243 2909 2243
Size: 666 6502
PstI C_TGCA'G
Cuts at: 830 4520 830
Size: 3690 3478
PvuI CG_AT'CG
Cuts at: 2001 2759 2001
Size: 758 6410
PvuII CAG'CTG
Cuts at: 2013 3832 2013
Size: 1819 5349
SalI G'TCGA_C
Cuts at: 1936 4510 1936
Size: 2574 4594
SapI GCTCTTCn'nnn_
Cuts at: 2106 2106
Size: 7168
SbfI CC_TGCA'GG
Cuts at: 4520 4520
Size: 7168
ScaI AGT'ACT
Cuts at: 2276 3761 3851 5416 7128 2276
Size: 1485 90 1565 1712 2316
Fragments arranged by size:
2316 1712 1565 1485 90
SfcI C'TryA_G
Cuts at: 826 1045 2340 4516 5651 6332 6523 826
Size: 219 1295 2176 1135 681 191 1471
Fragments arranged by size:
2176 1471 1295 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2293 2293
Size: 7168
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7168
SgrAI Cr'CCGG_yG
Cuts at: 1700 2948 1700
Size: 1248 5920
SmaI CCC'GGG
Cuts at: 1872 3751 4495 1872
Size: 1879 744 4545
Fragments arranged by size:
4545 1879 744
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2930 2939 5272 6173 6435 820
Size: 197 926 987 9 2333 901 262 1553
Fragments arranged by size:
2333 1553 987 926 901 262 197 9
SnaBI TAC'GTA
Cuts at: 493 4959 493
Size: 4466 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7168
SphI G_CATG'C
Cuts at: 1132 4229 4526 1132
Size: 3097 297 3774
Fragments arranged by size:
3774 3097 297
SspI AAT'ATT
Cuts at: 5 52 5092 5
Size: 47 5040 2081
Fragments arranged by size:
5040 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7168
StyI C'CwwG_G
Cuts at: 513 1065 2004 2046 3528 4607 513
Size: 552 939 42 1482 1079 3074
Fragments arranged by size:
3074 1482 1079 939 552 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5195 5212 5365 5550 5995 325
Size: 1255 3615 17 153 185 445 1498
Fragments arranged by size:
3615 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2274 2324 3759
Size: 274 80 33 51 1443 297 50 1435
Cuts at: 3759 3849 4200 5414 7126 96
Size: 90 351 1214 1712 138
Fragments arranged by size:
1712 1443 1435 1214 351 297 274 138
90 80 51 50 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3011 3043 7128 7160 3011
Size: 32 4085 32 3019
Fragments arranged by size:
4085 3019 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7168
XbaI T'CTAG_A
Cuts at: 4504 4504
Size: 7168
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2571 3331 3898 2571
Size: 760 567 5841
Fragments arranged by size:
5841 760 567
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7168
XmaI C'CCGG_G
Cuts at: 1870 3749 4493 1870
Size: 1879 744 4545
Fragments arranged by size:
4545 1879 744
XmnI GAAnn'nnTTC
Cuts at: 5297 5297
Size: 7168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4976 276
Size: 53 83 186 936 3442 2468
Fragments arranged by size:
3442 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
DraIII EcoICRI EcoNI FseI FspAI NruI NsiI PacI
PfoI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI