Restriction map for
pFN21AE2060
(Circular) (Six-base) MAPSORT of: pFN21AE2060.seq Check: 1976 from: 1 to: 6820
pFN21AE2060 6820 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3296 3427 3296
Size: 131 6689
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4630 278
Size: 53 83 186 936 3094 2468
Fragments arranged by size:
3094 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4163 1937
Size: 2226 4594
Acc65I G'GTAC_C
Cuts at: 4141 4141
Size: 6820
AclI AA'CG_TT
Cuts at: 1563 4947 5320 1563
Size: 3384 373 3063
Fragments arranged by size:
3384 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2229 2534 4883 6267 6535 1632
Size: 597 305 2349 1384 268 1917
Fragments arranged by size:
2349 1917 1384 597 305 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6623
AflIII A'CryG_T
Cuts at: 1203 3912 5719 6696 1203
Size: 2709 1807 977 1327
Fragments arranged by size:
2709 1807 1327 977
AgeI A'CCGG_T
Cuts at: 5651 5651
Size: 6820
AhdI GACnn_n'nnGTC
Cuts at: 5549 5549
Size: 6820
AleI CACnn'nnGTG
Cuts at: 1166 3578 4070 1166
Size: 2412 492 3916
Fragments arranged by size:
3916 2412 492
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6820
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2846 2878 2846
Size: 32 6788
AlwNI CAG_nnn'CTG
Cuts at: 2494 2974 3055 4563 6135 2494
Size: 480 81 1508 1572 3179
Fragments arranged by size:
3179 1572 1508 480 81
ApaI G_GGCC'C
Cuts at: 3475 3475
Size: 6820
ApaLI G'TGCA_C
Cuts at: 4877 6033 4877
Size: 1156 5664
ApoI r'AATT_y
Cuts at: 1493 2315 4129 4355 4507 6616 1493
Size: 822 1814 226 152 2109 1697
Fragments arranged by size:
2109 1814 1697 822 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5374 160
Size: 872 4342 1606
Fragments arranged by size:
4342 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6820
AvaI C'yCGr_G
Cuts at: 1870 1943 2302 4145 1870
Size: 73 359 1843 4545
Fragments arranged by size:
4545 1843 359 73
BamHI G'GATC_C
Cuts at: 2470 3492 4150 4517 2470
Size: 1022 658 367 4773
Fragments arranged by size:
4773 1022 658 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 4141 5596 618
Size: 325 217 627 239 2115 1455 1842
Fragments arranged by size:
2115 1842 1455 627 325 239 217
BanII G_rGCy'C
Cuts at: 1391 3475 3591 4139 1391
Size: 2084 116 548 4072
Fragments arranged by size:
4072 2084 548 116
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2173 2562 928
Size: 971 274 389 5186
Fragments arranged by size:
5186 971 389 274
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5011 5045 1903
Size: 34 3074 34 3678
Fragments arranged by size:
3678 3074 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2156 3218 3528 4712 5922 2156
Size: 1062 310 1184 1210 3054
Fragments arranged by size:
3054 1210 1184 1062 310
BclI T'GATC_A
Cuts at: 1548 2145 1548
Size: 597 6223
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2538 5431 136
Size: 107 122 71 631 605 866 2893 1525
Fragments arranged by size:
2893 1525 866 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2235 2772 6815 2235
Size: 537 4043 2240
Fragments arranged by size:
4043 2240 537
BlpI GC'TnA_GC
Cuts at: 4237 4237
Size: 6820
Bme1580I G_kGCm'C
Cuts at: 1790 2933 3475 4881 6037 1790
Size: 1143 542 1406 1156 2573
Fragments arranged by size:
2573 1406 1156 1143 542
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6820
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2288 2300 2476
Size: 416 53 83 399 24 871 12 176
Cuts at: 2476 4551 5509 442
Size: 2075 958 1753
Fragments arranged by size:
2075 1753 958 871 416 399 176 83
53 24 12
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2605 2648 2735 3707 5480 1239
Size: 980 386 43 87 972 1773 2579
Fragments arranged by size:
2579 1773 980 972 386 87 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2690 4945 5810 6108 2690
Size: 2255 865 298 3402
Fragments arranged by size:
3402 2255 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5483 882
Size: 617 3984 2219
Fragments arranged by size:
3984 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3093 4084 4611 493
Size: 711 1889 991 527 2702
Fragments arranged by size:
2702 1889 991 711 527
BsaBI GATnn'nnATC
Cuts at: 6814 6814
Size: 6820
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3948 4627 5009 5644
Size: 53 83 186 936 2415 679 382 635
Cuts at: 5644 275
Size: 1451
Fragments arranged by size:
2415 1451 936 679 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5253 5651 5925 6072 6483 5253
Size: 398 274 147 411 5590
Fragments arranged by size:
5590 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2254 2284 1178
Size: 30 165 30 851 30 5714
Fragments arranged by size:
5714 851 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2387 2480 3713 3818 4124 4127 1186
Size: 1201 93 1233 105 306 3 3879
Fragments arranged by size:
3879 1233 1201 306 105 93 3
BseYI C'CCAG_C
Cuts at: 2475 2934 3478 4005 6023 6519 2475
Size: 459 544 527 2018 496 2776
Fragments arranged by size:
2776 2018 544 527 496 459
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2602 2684 3274 4063 1163
Size: 573 866 82 590 789 3920
Fragments arranged by size:
3920 866 789 590 573 82
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3487 4270 5031 6059 1540
Size: 275 186 1486 783 761 1028 2301
Fragments arranged by size:
2301 1486 1028 783 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3591 4077 4881 4966 6037 3591
Size: 486 804 85 1071 4374
Fragments arranged by size:
4374 1071 804 486 85
BsmI GAATG_Cn'
Cuts at: 2563 3058 4331 4424 2563
Size: 495 1273 93 4959
Fragments arranged by size:
4959 1273 495 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2933 3475 3591 4077 4139 4881 4966
Size: 399 1143 542 116 486 62 742 85
Cuts at: 4966 6037 1391
Size: 1071 2174
Fragments arranged by size:
2174 1143 1071 742 542 486 399 116
85 62
BspEI T'CCGG_A
Cuts at: 6483 6483
Size: 6820
BspHI T'CATG_A
Cuts at: 3591 3817 4709 6439 3591
Size: 226 892 1730 3972
Fragments arranged by size:
3972 1730 892 226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2664 2982 3023 3156 3296 3427 4175 844
Size: 1820 318 41 133 140 131 748 3489
Fragments arranged by size:
3489 1820 748 318 140 133 131 41
BsrBI CCG'CTC
Cuts at: 1394 1823 2686 4707 1394
Size: 429 863 2021 3507
Fragments arranged by size:
3507 2021 863 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2851 3006 5315 5489 65
Size: 1183 1603 155 2309 174 1396
Fragments arranged by size:
2309 1603 1396 1183 174 155
BsrFI r'CCGG_y
Cuts at: 1700 1810 2029 5464 5651 1700
Size: 110 219 3435 187 2869
Fragments arranged by size:
3435 2869 219 187 110
BsrGI T'GTAC_A
Cuts at: 96 2595 3282 96
Size: 2499 687 3634
Fragments arranged by size:
3634 2499 687
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6820
BssSI C'ACGA_G
Cuts at: 4880 5892 4880
Size: 1012 5808
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4041 794
Size: 3247 3573
BstBI TT'CG_AA
Cuts at: 5624 5624
Size: 6820
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6820
BstXI CCAn_nnnn'nTGG
Cuts at: 3086 3181 3086
Size: 95 6725
BstYI r'GATC_y
Cuts at: 1972 2235 2470 2772 2854 3492 4150 4517 4902
Size: 263 235 302 82 638 658 367 385
Cuts at: 4902 4919 6371 6457 6469 6815 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 658 638 385 367 346 302
263 235 86 82 17 12
Bsu36I CC'TnA_GG
Cuts at: 3824 3824
Size: 6820
BtgI C'CryG_G
Cuts at: 513 1065 2157 3375 3454 4014 513
Size: 552 1092 1218 79 560 3319
Fragments arranged by size:
3319 1218 1092 560 552 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3661 487
Size: 683 56 2435 3646
Fragments arranged by size:
3646 2435 683 56
BtsI GCAGTG_nn'
Cuts at: 2749 4336 5130 5150 2749
Size: 1587 794 20 4419
Fragments arranged by size:
4419 1587 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6820
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3112 3147 529
Size: 35 2548 35 4202
Fragments arranged by size:
4202 2548 35 35
DraI TTT'AAA
Cuts at: 4124 4124
Size: 6820
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5827 809
Size: 507 4511 1802
Fragments arranged by size:
4511 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4011 4267 5156
Size: 54 1425 50 275 18 2181 256 889
Cuts at: 5156 6785 8
Size: 1629 43
Fragments arranged by size:
2181 1629 1425 889 275 256 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4267 1537
Size: 275 2455 4090
Fragments arranged by size:
4090 2455 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3579 4750 1353
Size: 2226 1171 3423
Fragments arranged by size:
3423 2226 1171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5407 5781 5927 387
Size: 1064 138 3818 374 146 1280
Fragments arranged by size:
3818 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3589 3589
Size: 6820
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2229 2534 2605 2648 2735 3707
Size: 393 587 10 305 71 43 87 972
Cuts at: 3707 4883 5480 6267 6535 1239
Size: 1176 597 787 268 1524
Fragments arranged by size:
1524 1176 972 787 597 587 393 305
268 87 71 43 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2208 2208
Size: 6820
EcoO109I rG'GnC_Cy
Cuts at: 2689 2820 3471 3472 3713 3988 2689
Size: 131 651 1 241 275 5521
Fragments arranged by size:
5521 651 275 241 131 1
EcoRI G'AATT_C
Cuts at: 4129 4507 4129
Size: 378 6442
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 6820
FspI TGC'GCA
Cuts at: 5326 5326
Size: 6820
HaeII r_GCGC'y
Cuts at: 1678 5967 1678
Size: 4289 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2083 2115 3105 3137 3887
Size: 32 135 32 511 32 990 32 750
Cuts at: 3887 3919 5461 5493 5535 5567 1373
Size: 32 1542 32 42 32 2626
Fragments arranged by size:
2626 1542 990 750 511 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4164 4410 669
Size: 988 281 2226 246 3079
Fragments arranged by size:
3079 2226 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6820
HpaI GTT'AAC
Cuts at: 4410 4410
Size: 6820
KpnI G_GTAC'C
Cuts at: 4145 4145
Size: 6820
MfeI C'AATT_G
Cuts at: 4419 4419
Size: 6820
MluI A'CGCG_T
Cuts at: 6696 6696
Size: 6820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2391 2491 5934 6118 1305
Size: 1086 100 3443 184 2007
Fragments arranged by size:
3443 2007 1086 184 100
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4013 10
Size: 54 1425 343 2181 2817
Fragments arranged by size:
2817 2181 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2678 3179 3576 3578 4070 4778 5137
Size: 648 1512 501 397 2 492 708 359
Cuts at: 5137 5296 518
Size: 159 2042
Fragments arranged by size:
2042 1512 708 648 501 492 397 359
159 2
MspA1I CmG'CkG
Cuts at: 1606 3071 3489 3503 4236 4913 6061 6306 1606
Size: 1465 418 14 733 677 1148 245 2120
Fragments arranged by size:
2120 1465 1148 733 677 418 245 14
NaeI GCC'GGC
Cuts at: 2031 2031
Size: 6820
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6268
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6820
NgoMIV G'CCGG_C
Cuts at: 2029 2029
Size: 6820
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6820
NotI GC'GGCC_GC
Cuts at: 4267 4267
Size: 6820
NspI r_CATG'y
Cuts at: 1132 1229 3916 4178 5723 1132
Size: 97 2687 262 1545 2229
Fragments arranged by size:
2687 2229 1545 262 97
PasI CC'CwG_GG
Cuts at: 1348 3709 1348
Size: 2361 4459
PciI A'CATG_T
Cuts at: 3912 5719 3912
Size: 1807 5013
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2425 3356 1108
Size: 1317 931 4572
Fragments arranged by size:
4572 1317 931
PfoI T'CCnGG_A
Cuts at: 2447 2712 2447
Size: 265 6555
PmeI GTTT'AAAC
Cuts at: 4124 4124
Size: 6820
PmlI CAC'GTG
Cuts at: 3093 3093
Size: 6820
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4889 4921 6402 6434 4889
Size: 32 1481 32 5275
Fragments arranged by size:
5275 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3713 3988 3713
Size: 275 6545
PshAI GACnn'nnGTC
Cuts at: 1613 3642 1613
Size: 2029 4791
PsiI TTA'TAA
Cuts at: 4390 4390
Size: 6820
PspOMI G'GGCC_C
Cuts at: 3471 3471
Size: 6820
PstI C_TGCA'G
Cuts at: 830 2045 2979 4172 830
Size: 1215 934 1193 3478
Fragments arranged by size:
3478 1215 1193 934
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6820
PvuII CAG'CTG
Cuts at: 3071 3503 3071
Size: 432 6388
SacI G_AGCT'C
Cuts at: 3591 3591
Size: 6820
SalI G'TCGA_C
Cuts at: 1936 4162 1936
Size: 2226 4594
SanDI GG'GwC_CC
Cuts at: 3713 3988 3713
Size: 275 6545
SapI GCTCTTCn'nnn_
Cuts at: 3579 3579
Size: 6820
SbfI CC_TGCA'GG
Cuts at: 4172 4172
Size: 6820
ScaI AGT'ACT
Cuts at: 3215 5068 6780 3215
Size: 1853 1712 3255
Fragments arranged by size:
3255 1853 1712
SexAI A'CCwGG_T
Cuts at: 3048 3048
Size: 6820
SfcI C'TryA_G
Cuts at: 826 1045 2041 2668 2768 2975 4168 5303 5984
Size: 219 996 627 100 207 1193 1135 681
Cuts at: 5984 6175 826
Size: 191 1471
Fragments arranged by size:
1471 1193 1135 996 681 627 219 207
191 100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6820
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6820
SmaI CCC'GGG
Cuts at: 1872 2304 4147 1872
Size: 432 1843 4545
Fragments arranged by size:
4545 1843 432
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2705 4924 5825 6087 820
Size: 197 926 762 2219 901 262 1553
Fragments arranged by size:
2219 1553 926 901 762 262 197
SnaBI TAC'GTA
Cuts at: 493 4611 493
Size: 4118 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6820
SphI G_CATG'C
Cuts at: 1132 4178 1132
Size: 3046 3774
SspI AAT'ATT
Cuts at: 5 52 4744 5
Size: 47 4692 2081
Fragments arranged by size:
4692 2081 47
StuI AGG'CCT
Cuts at: 1517 3860 1517
Size: 2343 4477
StyI C'CwwG_G
Cuts at: 513 1065 2326 3522 4259 513
Size: 552 1261 1196 737 3074
Fragments arranged by size:
3074 1261 1196 737 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2628 3945 3997 4847 4864 5017 5202
Size: 1255 1048 1317 52 850 17 153 185
Cuts at: 5202 5647 325
Size: 445 1498
Fragments arranged by size:
1498 1317 1255 1048 850 445 185 153
52 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2595 2746 3213
Size: 274 80 33 51 1443 618 151 467
Cuts at: 3213 3282 5066 6778 96
Size: 69 1784 1712 138
Fragments arranged by size:
1784 1712 1443 618 467 274 151 138
80 69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2154 2186 2254 2286 3502 3534 3697 3729 6780
Size: 32 68 32 1216 32 163 32 3051
Cuts at: 6780 6812 2154
Size: 32 2162
Fragments arranged by size:
3051 2162 1216 163 68 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6820
XbaI T'CTAG_A
Cuts at: 4156 4156
Size: 6820
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3962 3962
Size: 6820
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6820
XmaI C'CCGG_G
Cuts at: 1870 2302 4145 1870
Size: 432 1843 4545
Fragments arranged by size:
4545 1843 432
XmnI GAAnn'nnTTC
Cuts at: 4949 4949
Size: 6820
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4628 276
Size: 53 83 186 936 3094 2468
Fragments arranged by size:
3094 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PspXI PsrI RsrII
SacII SfiI SfoI SrfI SwaI