Restriction map for
pFN21AE2059
(Circular) (Six-base) MAPSORT of: pFN21AE2059.seq Check: 2439 from: 1 to: 6808
pFN21AE2059 6808 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3207 3731 3207
Size: 524 6284
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4618 278
Size: 53 83 186 936 3082 2468
Fragments arranged by size:
3082 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4151 1937
Size: 2214 4594
Acc65I G'GTAC_C
Cuts at: 2799 4129 2799
Size: 1330 5478
AclI AA'CG_TT
Cuts at: 1563 4935 5308 1563
Size: 3372 373 3063
Fragments arranged by size:
3372 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2732 4871 6255 6523 1632
Size: 1100 2139 1384 268 1917
Fragments arranged by size:
2139 1917 1384 1100 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6611
AflIII A'CryG_T
Cuts at: 1203 3855 5707 6684 1203
Size: 2652 1852 977 1327
Fragments arranged by size:
2652 1852 1327 977
AgeI A'CCGG_T
Cuts at: 5639 5639
Size: 6808
AhdI GACnn_n'nnGTC
Cuts at: 2047 5537 2047
Size: 3490 3318
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6808
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2279 2289 3074 3905 1184
Size: 1095 10 785 831 4087
Fragments arranged by size:
4087 1095 831 785 10
AlwNI CAG_nnn'CTG
Cuts at: 2116 2185 3481 4009 4551 6123 2116
Size: 69 1296 528 542 1572 2801
Fragments arranged by size:
2801 1572 1296 542 528 69
ApaI G_GGCC'C
Cuts at: 2652 2652
Size: 6808
ApaLI G'TGCA_C
Cuts at: 2474 4865 6021 2474
Size: 2391 1156 3261
Fragments arranged by size:
3261 2391 1156
ApoI r'AATT_y
Cuts at: 1493 2727 4117 4343 4495 6604 1493
Size: 1234 1390 226 152 2109 1697
Fragments arranged by size:
2109 1697 1390 1234 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5362 160
Size: 872 4330 1606
Fragments arranged by size:
4330 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6808
AvaI C'yCGr_G
Cuts at: 1870 1943 3044 3568 4133 1870
Size: 73 1101 524 565 4545
Fragments arranged by size:
4545 1101 565 524 73
AvrII C'CTAG_G
Cuts at: 3665 3665
Size: 6808
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2272 2305 2272
Size: 33 6775
BamHI G'GATC_C
Cuts at: 2850 4138 4505 2850
Size: 1288 367 5153
Fragments arranged by size:
5153 1288 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2799 4129 5584 618
Size: 325 217 627 249 763 1330 1455 1842
Fragments arranged by size:
1842 1455 1330 763 627 325 249 217
BanII G_rGCy'C
Cuts at: 1391 2196 2652 2991 3357 3415 3426 3656 4127
Size: 805 456 339 366 58 11 230 471
Cuts at: 4127 1391
Size: 4072
Fragments arranged by size:
4072 805 471 456 366 339 230 58
11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3878 928
Size: 971 1979 3858
Fragments arranged by size:
3858 1979 971
BbvCI CC'TCA_GC
Cuts at: 3222 3245 3908 3222
Size: 23 663 6122
Fragments arranged by size:
6122 663 23
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2358 2392 3173 3207 4999 5033 1903
Size: 34 421 34 781 34 1792 34 3678
Fragments arranged by size:
3678 1792 781 421 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4700 5910 4700
Size: 1210 5598
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6808
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5419 136
Size: 107 122 71 631 605 3747 1525
Fragments arranged by size:
3747 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2268 4017 6803 2268
Size: 1749 2786 2273
Fragments arranged by size:
2786 2273 1749
BlpI GC'TnA_GC
Cuts at: 2990 3077 4225 2990
Size: 87 1148 5573
Fragments arranged by size:
5573 1148 87
Bme1580I G_kGCm'C
Cuts at: 1790 2177 2278 2478 2652 4869 6025 1790
Size: 387 101 200 174 2217 1156 2573
Fragments arranged by size:
2573 2217 1156 387 200 174 101
BmgBI CAC'GTC
Cuts at: 1318 3153 1318
Size: 1835 4973
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2154 2478 2785
Size: 416 53 83 399 24 737 324 307
Cuts at: 2785 3111 4539 5497 442
Size: 326 1428 958 1753
Fragments arranged by size:
1753 1428 958 737 416 399 326 324
307 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3146 1056
Size: 2090 4718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2414 2948 3224 3551 3860 5468 1239
Size: 1175 534 276 327 309 1608 2579
Fragments arranged by size:
2579 1608 1175 534 327 309 276
Bpu10I CC'TnA_GC
Cuts at: 3222 3245 3655 3687 3908 3222
Size: 23 410 32 221 6122
Fragments arranged by size:
6122 410 221 32 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2603 4933 5798 6096 2603
Size: 2330 865 298 3315
Fragments arranged by size:
3315 2330 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2324 3836 5471 882
Size: 617 825 1512 1635 2219
Fragments arranged by size:
2219 1635 1512 825 617
BsaAI yAC'GTr
Cuts at: 493 1204 2764 3879 4599 493
Size: 711 1560 1115 720 2702
Fragments arranged by size:
2702 1560 1115 720 711
BsaBI GATnn'nnATC
Cuts at: 3062 6802 3062
Size: 3740 3068
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2817 4615 4997 5632
Size: 53 83 186 936 1284 1798 382 635
Cuts at: 5632 275
Size: 1451
Fragments arranged by size:
1798 1451 1284 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2497 3595 5241 5639 5913 6060 6471 2497
Size: 1098 1646 398 274 147 411 2834
Fragments arranged by size:
2834 1646 1098 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6583
Fragments arranged by size:
6583 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2423 3272 3329 3983 3997 1186
Size: 1237 849 57 654 14 3997
Fragments arranged by size:
3997 1237 849 654 57 14
BseYI C'CCAG_C
Cuts at: 2465 3395 3790 6011 6507 2465
Size: 930 395 2221 496 2766
Fragments arranged by size:
2766 2221 930 496 395
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2189 2195 2285 2827 3017 3635 1163
Size: 573 453 6 90 542 190 618 4336
Fragments arranged by size:
4336 618 573 542 453 190 90 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2287 2998 4258 5019 6047 1540
Size: 275 186 286 711 1260 761 1028 2301
Fragments arranged by size:
2301 1260 1028 761 711 286 275 186
BsiHKAI G_wGCw'C
Cuts at: 2196 2400 2478 2775 2991 3426 3656 3900 4869
Size: 204 78 297 216 435 230 244 969
Cuts at: 4869 4954 6025 2196
Size: 85 1071 2979
Fragments arranged by size:
2979 1071 969 435 297 244 230 216
204 85 78
BsmI GAATG_Cn'
Cuts at: 3529 4319 4412 3529
Size: 790 93 5925
Fragments arranged by size:
5925 790 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2758 2758
Size: 6808
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 2196 2278 2400 2478 2652 2775
Size: 399 387 19 82 122 78 174 123
Cuts at: 2775 2991 3357 3415 3426 3656 3900 4127 4869
Size: 216 366 58 11 230 244 227 742
Cuts at: 4869 4954 6025 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 366 244 230
227 216 174 123 122 85 82 78
58 19 11
BspEI T'CCGG_A
Cuts at: 3595 6471 3595
Size: 2876 3932
BspHI T'CATG_A
Cuts at: 4697 6427 4697
Size: 1730 5078
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3207 3261 3489 3731 4163 844
Size: 2363 54 228 242 432 3489
Fragments arranged by size:
3489 2363 432 242 228 54
BsrBI CCG'CTC
Cuts at: 1394 1823 2221 4695 1394
Size: 429 398 2474 3507
Fragments arranged by size:
3507 2474 429 398
BsrDI GCAATG_nn'
Cuts at: 65 1248 3189 3216 5303 5477 65
Size: 1183 1941 27 2087 174 1396
Fragments arranged by size:
2087 1941 1396 1183 174 27
BsrFI r'CCGG_y
Cuts at: 1700 1810 2523 5452 5639 1700
Size: 110 713 2929 187 2869
Fragments arranged by size:
2929 2869 713 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6808
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6808
BssSI C'ACGA_G
Cuts at: 3182 4868 5880 3182
Size: 1686 1012 4110
Fragments arranged by size:
4110 1686 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4047 794
Size: 3253 3555
BstBI TT'CG_AA
Cuts at: 5612 5612
Size: 6808
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6808
BstXI CCAn_nnnn'nTGG
Cuts at: 2659 3089 2659
Size: 430 6378
BstYI r'GATC_y
Cuts at: 1972 2268 2850 4017 4138 4505 4890 4907 6359
Size: 296 582 1167 121 367 385 17 1452
Cuts at: 6359 6445 6457 6803 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1167 582 385 367 346 296
121 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2178 2178
Size: 6808
BtgI C'CryG_G
Cuts at: 513 1065 2004 2652 2674 2982 3582 3977 513
Size: 552 939 648 22 308 600 395 3344
Fragments arranged by size:
3344 939 648 600 552 395 308 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2495 3049 3516 487
Size: 683 56 1269 554 467 3779
Fragments arranged by size:
3779 1269 683 554 467 56
BtsI GCAGTG_nn'
Cuts at: 2177 3010 4324 5118 5138 2177
Size: 833 1314 794 20 3847
Fragments arranged by size:
3847 1314 833 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6808
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6773
DraI TTT'AAA
Cuts at: 3628 4112 3628
Size: 484 6324
DraIII CAC_nnn'GTG
Cuts at: 3964 3964
Size: 6808
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5815 809
Size: 507 4499 1802
Fragments arranged by size:
4499 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2028 2284
Size: 54 1425 50 275 18 177 21 256
Cuts at: 2284 2766 2949 2995 3325 4255 5144 6773 8
Size: 482 183 46 330 930 889 1629 43
Fragments arranged by size:
1629 1425 930 889 482 330 275 256
183 177 54 50 46 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2284 2995 4255 1537
Size: 275 472 711 1260 4090
Fragments arranged by size:
4090 1260 711 472 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2699 4738 1353
Size: 1346 2039 3423
Fragments arranged by size:
3423 2039 1346
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2195 2630 2825 3989 4022 5395
Size: 1064 138 606 435 195 1164 33 1373
Cuts at: 5395 5769 5915 387
Size: 374 146 1280
Fragments arranged by size:
1373 1280 1164 1064 606 435 374 195
146 138 33
EcoICRI GAG'CTC
Cuts at: 2194 2989 3424 3654 2194
Size: 795 435 230 5348
Fragments arranged by size:
5348 795 435 230
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2414 2732 2948 3224 3551 3860 4871
Size: 393 782 318 216 276 327 309 1011
Cuts at: 4871 5468 6255 6523 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1011 787 782 597 393 327 318
309 276 268 216
EcoNI CCTnn'n_nnAGG
Cuts at: 2900 3244 3344 2900
Size: 344 100 6364
Fragments arranged by size:
6364 344 100
EcoO109I rG'GnC_Cy
Cuts at: 2182 2242 2648 3478 3973 2182
Size: 60 406 830 495 5017
Fragments arranged by size:
5017 830 495 406 60
EcoRI G'AATT_C
Cuts at: 4117 4495 4117
Size: 378 6430
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2446 2446
Size: 6808
FspI TGC'GCA
Cuts at: 5314 5314
Size: 6808
HaeII r_GCGC'y
Cuts at: 1678 4009 5955 1678
Size: 2331 1946 2531
Fragments arranged by size:
2531 2331 1946
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2054 2065 2086 3291
Size: 32 135 32 461 21 11 21 1205
Cuts at: 3291 3323 5449 5481 5523 5555 1373
Size: 32 2126 32 42 32 2626
Fragments arranged by size:
2626 2126 1205 461 135 42 32 32
32 32 32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2821 3748 4152 4398 669
Size: 988 281 883 927 404 246 3079
Fragments arranged by size:
3079 988 927 883 404 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6808
HpaI GTT'AAC
Cuts at: 4398 4398
Size: 6808
KpnI G_GTAC'C
Cuts at: 2803 4133 2803
Size: 1330 5478
MfeI C'AATT_G
Cuts at: 4407 4407
Size: 6808
MluI A'CGCG_T
Cuts at: 6684 6684
Size: 6808
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2664 3399 5922 6106 1305
Size: 1359 735 2523 184 2007
Fragments arranged by size:
2523 2007 1359 735 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2030 10
Size: 54 1425 343 177 21 4788
Fragments arranged by size:
4788 1425 343 177 54 21
MslI CAynn'nnrTG
Cuts at: 518 1166 4766 5125 5284 518
Size: 648 3600 359 159 2042
Fragments arranged by size:
3600 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2113 2676 2841 2994 3979 4224 4901 6049
Size: 507 563 165 153 985 245 677 1148
Cuts at: 6049 6294 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 985 677 563 507 245 245
165 153
NcoI C'CATG_G
Cuts at: 513 1065 2004 2652 513
Size: 552 939 648 4669
Fragments arranged by size:
4669 939 648 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6808
NheI G'CTAG_C
Cuts at: 1052 3142 1052
Size: 2090 4718
NotI GC'GGCC_GC
Cuts at: 4255 4255
Size: 6808
NspI r_CATG'y
Cuts at: 1132 1229 3373 4166 5711 1132
Size: 97 2144 793 1545 2229
Fragments arranged by size:
2229 2144 1545 793 97
PasI CC'CwG_GG
Cuts at: 1348 3089 3376 3937 1348
Size: 1741 287 561 4219
Fragments arranged by size:
4219 1741 561 287
PciI A'CATG_T
Cuts at: 5707 5707
Size: 6808
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2317 3153 3202 1108
Size: 1209 836 49 4714
Fragments arranged by size:
4714 1209 836 49
PfoI T'CCnGG_A
Cuts at: 2343 2391 2548 3474 3948 2343
Size: 48 157 926 474 5203
Fragments arranged by size:
5203 926 474 157 48
PmeI GTTT'AAAC
Cuts at: 4112 4112
Size: 6808
PmlI CAC'GTG
Cuts at: 3879 3879
Size: 6808
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4004 4036 4877 4909 6390 6422 4004
Size: 32 841 32 1481 32 4390
Fragments arranged by size:
4390 1481 841 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2182 2242 3478 2182
Size: 60 1236 5512
Fragments arranged by size:
5512 1236 60
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6808
PsiI TTA'TAA
Cuts at: 4378 4378
Size: 6808
PspOMI G'GGCC_C
Cuts at: 2648 2648
Size: 6808
PstI C_TGCA'G
Cuts at: 830 4160 830
Size: 3330 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6808
PvuII CAG'CTG
Cuts at: 2113 2113
Size: 6808
SacI G_AGCT'C
Cuts at: 2196 2991 3426 3656 2196
Size: 795 435 230 5348
Fragments arranged by size:
5348 795 435 230
SacII CC_GC'GG
Cuts at: 2677 3980 2677
Size: 1303 5505
SalI G'TCGA_C
Cuts at: 1936 4150 1936
Size: 2214 4594
SbfI CC_TGCA'GG
Cuts at: 4160 4160
Size: 6808
ScaI AGT'ACT
Cuts at: 5056 6768 5056
Size: 1712 5096
SfcI C'TryA_G
Cuts at: 826 1045 3175 3708 4156 5291 5972 6163 826
Size: 219 2130 533 448 1135 681 191 1471
Fragments arranged by size:
2130 1471 1135 681 533 448 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6808
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6808
SmaI CCC'GGG
Cuts at: 1872 4135 1872
Size: 2263 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2618 4912 5813 6075 820
Size: 197 926 675 2294 901 262 1553
Fragments arranged by size:
2294 1553 926 901 675 262 197
SnaBI TAC'GTA
Cuts at: 493 4599 493
Size: 4106 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6808
SphI G_CATG'C
Cuts at: 1132 4166 1132
Size: 3034 3774
SspI AAT'ATT
Cuts at: 5 52 3624 4732 5
Size: 47 3572 1108 2081
Fragments arranged by size:
3572 2081 1108 47
StuI AGG'CCT
Cuts at: 1517 3227 1517
Size: 1710 5098
StyI C'CwwG_G
Cuts at: 513 1065 2004 2238 2246 2652 3363 3665 4247
Size: 552 939 234 8 406 711 302 582
Cuts at: 4247 513
Size: 3074
Fragments arranged by size:
3074 939 711 582 552 406 302 234
8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2177 3601 4835 4852 5005 5190 5635
Size: 1255 597 1424 1234 17 153 185 445
Cuts at: 5635 325
Size: 1498
Fragments arranged by size:
1498 1424 1255 1234 597 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2160 5054 6766
Size: 274 80 33 51 1443 183 2894 1712
Cuts at: 6766 96
Size: 138
Fragments arranged by size:
2894 1712 1443 274 183 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4014 4046 6768 6800 4014
Size: 32 2722 32 4022
Fragments arranged by size:
4022 2722 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6808
XbaI T'CTAG_A
Cuts at: 4144 4144
Size: 6808
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2318 3457 3482 2318
Size: 1139 25 5644
Fragments arranged by size:
5644 1139 25
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6808
XmaI C'CCGG_G
Cuts at: 1870 4133 1870
Size: 2263 4545
XmnI GAAnn'nnTTC
Cuts at: 2729 4937 2729
Size: 2208 4600
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4616 276
Size: 53 83 186 936 3082 2468
Fragments arranged by size:
3082 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BfrBI BplI BsiWI BstZ17I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PspXI PsrI RsrII SanDI SapI SexAI
SfiI SfoI SrfI SwaI