Restriction map for
pFN21AE2058
(Circular) (Six-base) MAPSORT of: pFN21AE2058.seq Check: 6299 from: 1 to: 6799
pFN21AE2058 6799 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4609 278
Size: 53 83 186 936 3073 2468
Fragments arranged by size:
3073 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3249 4093 4142 1937
Size: 1312 844 49 4594
Fragments arranged by size:
4594 1312 844 49
Acc65I G'GTAC_C
Cuts at: 3758 4120 3758
Size: 362 6437
AclI AA'CG_TT
Cuts at: 1563 4926 5299 1563
Size: 3363 373 3063
Fragments arranged by size:
3363 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2312 2666 4862 6246 6514 1632
Size: 680 354 2196 1384 268 1917
Fragments arranged by size:
2196 1917 1384 680 354 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6602
AflIII A'CryG_T
Cuts at: 1203 5698 6675 1203
Size: 4495 977 1327
Fragments arranged by size:
4495 1327 977
AgeI A'CCGG_T
Cuts at: 5630 5630
Size: 6799
AhdI GACnn_n'nnGTC
Cuts at: 5528 5528
Size: 6799
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6799
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6799
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3935 3967 3935
Size: 32 6767
AlwNI CAG_nnn'CTG
Cuts at: 3371 3543 4542 6114 3371
Size: 172 999 1572 4056
Fragments arranged by size:
4056 1572 999 172
ApaLI G'TGCA_C
Cuts at: 4856 6012 4856
Size: 1156 5643
ApoI r'AATT_y
Cuts at: 1493 2903 3494 4108 4334 4486 6595 1493
Size: 1410 591 614 226 152 2109 1697
Fragments arranged by size:
2109 1697 1410 614 591 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5353 160
Size: 872 4321 1606
Fragments arranged by size:
4321 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6799
AvaI C'yCGr_G
Cuts at: 1870 1943 2524 3907 4124 1870
Size: 73 581 1383 217 4545
Fragments arranged by size:
4545 1383 581 217 73
BamHI G'GATC_C
Cuts at: 2329 4129 4496 2329
Size: 1800 367 4632
Fragments arranged by size:
4632 1800 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3274 3758 4120 5575 618
Size: 325 217 627 1487 484 362 1455 1842
Fragments arranged by size:
1842 1487 1455 627 484 362 325 217
BanII G_rGCy'C
Cuts at: 1391 2026 3563 3808 4118 1391
Size: 635 1537 245 310 4072
Fragments arranged by size:
4072 1537 635 310 245
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3192 928
Size: 971 1293 4535
Fragments arranged by size:
4535 1293 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2216 2250 4990 5024 1903
Size: 34 279 34 2740 34 3678
Fragments arranged by size:
3678 2740 279 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3313 3593 4691 5901 3313
Size: 280 1098 1210 4211
Fragments arranged by size:
4211 1210 1098 280
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6799
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2219 5410 136
Size: 107 122 71 631 605 547 3191 1525
Fragments arranged by size:
3191 1525 631 605 547 122 107 71
BglII A'GATC_T
Cuts at: 6794 6794
Size: 6799
BlpI GC'TnA_GC
Cuts at: 3101 4216 3101
Size: 1115 5684
Bme1580I G_kGCm'C
Cuts at: 1790 2539 3517 4860 6016 1790
Size: 749 978 1343 1156 2573
Fragments arranged by size:
2573 1343 1156 978 749
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6799
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2160 3696 4530
Size: 416 53 83 399 24 743 1536 834
Cuts at: 4530 5488 442
Size: 958 1753
Fragments arranged by size:
1753 1536 958 834 743 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3520 5459 1239
Size: 2281 1939 2579
Fragments arranged by size:
2579 2281 1939
Bpu10I CC'TnA_GC
Cuts at: 2481 3562 2481
Size: 1081 5718
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2519 3104 4924 5789 6087 2519
Size: 585 1820 865 298 3231
Fragments arranged by size:
3231 1820 865 585 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3170 5462 882
Size: 617 1671 2292 2219
Fragments arranged by size:
2292 2219 1671 617
BsaAI yAC'GTr
Cuts at: 493 1204 2489 4590 493
Size: 711 1285 2101 2702
Fragments arranged by size:
2702 2101 1285 711
BsaBI GATnn'nnATC
Cuts at: 6793 6793
Size: 6799
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4606 4988 5623 275
Size: 53 83 186 936 3073 382 635 1451
Fragments arranged by size:
3073 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3030 3927 5232 5630 5904 6051 6462 3030
Size: 897 1305 398 274 147 411 3367
Fragments arranged by size:
3367 1305 897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3231 3261 1178
Size: 30 165 30 1828 30 4716
Fragments arranged by size:
4716 1828 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2913 3791 1186
Size: 1727 878 4194
Fragments arranged by size:
4194 1727 878
BseYI C'CCAG_C
Cuts at: 2438 2565 2657 6002 6498 2438
Size: 127 92 3345 496 2739
Fragments arranged by size:
3345 2739 496 127 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2109 4066 1163
Size: 573 373 1957 3896
Fragments arranged by size:
3896 1957 573 373
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3932 4249 5010 6038 1540
Size: 275 186 1931 317 761 1028 2301
Fragments arranged by size:
2301 1931 1028 761 317 275 186
BsiHKAI G_wGCw'C
Cuts at: 4860 4945 6016 4860
Size: 85 1071 5643
Fragments arranged by size:
5643 1071 85
BsmI GAATG_Cn'
Cuts at: 3080 4310 4403 3080
Size: 1230 93 5476
Fragments arranged by size:
5476 1230 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2539 3517 3563 3808 4118 4860
Size: 399 236 513 978 46 245 310 742
Cuts at: 4860 4945 6016 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 978 742 513 399 310 245
236 85 46
BspEI T'CCGG_A
Cuts at: 3030 6462 3030
Size: 3432 3367
BspHI T'CATG_A
Cuts at: 3204 3985 4688 6418 3204
Size: 781 703 1730 3585
Fragments arranged by size:
3585 1730 781 703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2243 2421 2946 4154 844
Size: 1399 178 525 1208 3489
Fragments arranged by size:
3489 1399 1208 525 178
BsrBI CCG'CTC
Cuts at: 1394 1823 4686 1394
Size: 429 2863 3507
Fragments arranged by size:
3507 2863 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2214 5294 5468 65
Size: 1183 966 3080 174 1396
Fragments arranged by size:
3080 1396 1183 966 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2761 5443 5630 1700
Size: 110 951 2682 187 2869
Fragments arranged by size:
2869 2682 951 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6799
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6799
BssSI C'ACGA_G
Cuts at: 3110 4859 5871 3110
Size: 1749 1012 4038
Fragments arranged by size:
4038 1749 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6799
BstBI TT'CG_AA
Cuts at: 5603 5603
Size: 6799
BstEII G'GTnAC_C
Cuts at: 1184 4040 1184
Size: 2856 3943
BstXI CCAn_nnnn'nTGG
Cuts at: 3232 3232
Size: 6799
BstYI r'GATC_y
Cuts at: 1972 2329 4129 4496 4881 4898 6350 6436 6448
Size: 357 1800 367 385 17 1452 86 12
Cuts at: 6448 6794 1972
Size: 346 1977
Fragments arranged by size:
1977 1800 1452 385 367 357 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2016 2959 3450 3707 513
Size: 552 951 943 491 257 3605
Fragments arranged by size:
3605 951 943 552 491 257
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3178 487
Size: 683 56 1952 4108
Fragments arranged by size:
4108 1952 683 56
BtsI GCAGTG_nn'
Cuts at: 2097 2514 3021 3357 3478 4315 5109 5129 2097
Size: 417 507 336 121 837 794 20 3767
Fragments arranged by size:
3767 837 794 507 417 336 121 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6799
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6764
DraI TTT'AAA
Cuts at: 2970 4103 2970
Size: 1133 5666
DraIII CAC_nnn'GTG
Cuts at: 2115 3820 2115
Size: 1705 5094
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5806 809
Size: 507 4490 1802
Fragments arranged by size:
4490 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2511 3798 3975
Size: 54 1425 50 275 18 681 1287 177
Cuts at: 3975 4246 5135 6764 8
Size: 271 889 1629 43
Fragments arranged by size:
1629 1425 1287 889 681 275 271 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4246 1537
Size: 275 2434 4090
Fragments arranged by size:
4090 2434 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4729 1353
Size: 3376 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5386 5760 5906 387
Size: 1064 138 3797 374 146 1280
Fragments arranged by size:
3797 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2666 3520 4862 5459 6246 6514
Size: 393 680 354 854 1342 597 787 268
Cuts at: 6514 1239
Size: 1524
Fragments arranged by size:
1524 1342 854 787 680 597 393 354
268
EcoO109I rG'GnC_Cy
Cuts at: 2797 2935 3943 2797
Size: 138 1008 5653
Fragments arranged by size:
5653 1008 138
EcoRI G'AATT_C
Cuts at: 2903 4108 4486 2903
Size: 1205 378 5216
Fragments arranged by size:
5216 1205 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2860 2860
Size: 6799
FspI TGC'GCA
Cuts at: 5305 5305
Size: 6799
HaeII r_GCGC'y
Cuts at: 1678 3621 5946 1678
Size: 1943 2325 2531
Fragments arranged by size:
2531 2325 1943
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3152 3184 3231 3263 3850
Size: 32 135 32 1580 32 47 32 587
Cuts at: 3850 3882 5440 5472 5514 5546 1373
Size: 32 1558 32 42 32 2626
Fragments arranged by size:
2626 1580 1558 587 135 47 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2576 3142 3869 4143 4389 669
Size: 988 281 638 566 727 274 246 3079
Fragments arranged by size:
3079 988 727 638 566 281 274 246
HindIII A'AGCT_T
Cuts at: 748 3843 748
Size: 3095 3704
HpaI GTT'AAC
Cuts at: 3142 4389 3142
Size: 1247 5552
KpnI G_GTAC'C
Cuts at: 3762 4124 3762
Size: 362 6437
MfeI C'AATT_G
Cuts at: 4398 4398
Size: 6799
MluI A'CGCG_T
Cuts at: 6675 6675
Size: 6799
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2076 2932 3912 5913 6097 1305
Size: 771 856 980 2001 184 2007
Fragments arranged by size:
2007 2001 980 856 771 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2513 3800 3977 10
Size: 54 1425 343 681 1287 177 2832
Fragments arranged by size:
2832 1425 1287 681 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3449 4757 5116 5275 518
Size: 648 2283 1308 359 159 2042
Fragments arranged by size:
2283 2042 1308 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2438 2532 2569 3434 4215 4892 6040 6285
Size: 832 94 37 865 781 677 1148 245
Cuts at: 6285 1606
Size: 2120
Fragments arranged by size:
2120 1148 865 832 781 677 245 94
37
NcoI C'CATG_G
Cuts at: 513 1065 3450 3707 513
Size: 552 2385 257 3605
Fragments arranged by size:
3605 2385 552 257
NdeI CA'TA_TG
Cuts at: 387 2136 4000 387
Size: 1749 1864 3186
Fragments arranged by size:
3186 1864 1749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6799
NotI GC'GGCC_GC
Cuts at: 4246 4246
Size: 6799
NspI r_CATG'y
Cuts at: 1132 1229 3054 4157 5702 1132
Size: 97 1825 1103 1545 2229
Fragments arranged by size:
2229 1825 1545 1103 97
PasI CC'CwG_GG
Cuts at: 1348 3518 1348
Size: 2170 4629
PciI A'CATG_T
Cuts at: 5698 5698
Size: 6799
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2159 2591 1108
Size: 1051 432 5316
Fragments arranged by size:
5316 1051 432
PfoI T'CCnGG_A
Cuts at: 4071 4071
Size: 6799
PmeI GTTT'AAAC
Cuts at: 4103 4103
Size: 6799
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4868 4900 6381 6413 4868
Size: 32 1481 32 5254
Fragments arranged by size:
5254 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2797 2935 3943 2797
Size: 138 1008 5653
Fragments arranged by size:
5653 1008 138
PshAI GACnn'nnGTC
Cuts at: 1613 3537 1613
Size: 1924 4875
PsiI TTA'TAA
Cuts at: 4369 4369
Size: 6799
PstI C_TGCA'G
Cuts at: 830 3485 4151 830
Size: 2655 666 3478
Fragments arranged by size:
3478 2655 666
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6799
PvuII CAG'CTG
Cuts at: 2532 2569 2532
Size: 37 6762
SalI G'TCGA_C
Cuts at: 1936 4141 1936
Size: 2205 4594
SbfI CC_TGCA'GG
Cuts at: 4151 4151
Size: 6799
ScaI AGT'ACT
Cuts at: 5047 6759 5047
Size: 1712 5087
SfcI C'TryA_G
Cuts at: 826 1045 3345 3481 4147 5282 5963 6154 826
Size: 219 2300 136 666 1135 681 191 1471
Fragments arranged by size:
2300 1471 1135 681 666 219 191 136
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6799
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6799
SmaI CCC'GGG
Cuts at: 1872 4126 1872
Size: 2254 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2498 3119 4903 5804 6066 820
Size: 197 926 555 621 1784 901 262 1553
Fragments arranged by size:
1784 1553 926 901 621 555 262 197
SnaBI TAC'GTA
Cuts at: 493 4590 493
Size: 4097 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6799
SphI G_CATG'C
Cuts at: 1132 4157 1132
Size: 3025 3774
SspI AAT'ATT
Cuts at: 5 52 4723 5
Size: 47 4671 2081
Fragments arranged by size:
4671 2081 47
StuI AGG'CCT
Cuts at: 1517 3567 1517
Size: 2050 4749
StyI C'CwwG_G
Cuts at: 513 1065 3450 3707 3939 4238 513
Size: 552 2385 257 232 299 3074
Fragments arranged by size:
3074 2385 552 299 257 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2430 4826 4843 4996 5181 5626 325
Size: 1255 850 2396 17 153 185 445 1498
Fragments arranged by size:
2396 1498 1255 850 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5045 6757 96
Size: 274 80 33 51 1443 3068 1712 138
Fragments arranged by size:
3068 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6759 6791 6759
Size: 32 6767
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6799
XbaI T'CTAG_A
Cuts at: 4135 4135
Size: 6799
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2879 3001 3419 3769 2879
Size: 122 418 350 5909
Fragments arranged by size:
5909 418 350 122
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6799
XmaI C'CCGG_G
Cuts at: 1870 4124 1870
Size: 2254 4545
XmnI GAAnn'nnTTC
Cuts at: 4928 4928
Size: 6799
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4607 276
Size: 53 83 186 936 3073 2468
Fragments arranged by size:
3073 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI