Restriction map for
pFN21AE2049
(Circular) (Six-base) MAPSORT of: pFN21AE2049.seq Check: 7472 from: 1 to: 6718
pFN21AE2049 6718 bp
With 182 enzymes: SgfI *
June 3, 2018 17:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2607 4528 278
Size: 53 83 186 936 1071 1921 2468
Fragments arranged by size:
2468 1921 1071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4061 1937
Size: 2124 4594
Acc65I G'GTAC_C
Cuts at: 4039 4039
Size: 6718
AclI AA'CG_TT
Cuts at: 1563 4017 4845 5218 1563
Size: 2454 828 373 3063
Fragments arranged by size:
3063 2454 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2441 4781 6165 6433 1632
Size: 809 2340 1384 268 1917
Fragments arranged by size:
2340 1917 1384 809 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6521
AflIII A'CryG_T
Cuts at: 1203 2016 5617 6594 1203
Size: 813 3601 977 1327
Fragments arranged by size:
3601 1327 977 813
AgeI A'CCGG_T
Cuts at: 5549 5549
Size: 6718
AhdI GACnn_n'nnGTC
Cuts at: 3245 5447 3245
Size: 2202 4516
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6718
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2321 1184
Size: 1137 5581
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3442 3474 3442
Size: 32 6686
AlwNI CAG_nnn'CTG
Cuts at: 3521 4461 6033 3521
Size: 940 1572 4206
Fragments arranged by size:
4206 1572 940
ApaI G_GGCC'C
Cuts at: 2157 2157
Size: 6718
ApaLI G'TGCA_C
Cuts at: 3350 4775 5931 3350
Size: 1425 1156 4137
Fragments arranged by size:
4137 1425 1156
ApoI r'AATT_y
Cuts at: 1493 2855 3004 3286 4027 4253 4405 6514 1493
Size: 1362 149 282 741 226 152 2109 1697
Fragments arranged by size:
2109 1697 1362 741 282 226 152 149
AseI AT'TA_AT
Cuts at: 160 1032 5272 160
Size: 872 4240 1606
Fragments arranged by size:
4240 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6718
AvaI C'yCGr_G
Cuts at: 1870 1943 2588 4043 1870
Size: 73 645 1455 4545
Fragments arranged by size:
4545 1455 645 73
BamHI G'GATC_C
Cuts at: 3442 4048 4415 3442
Size: 606 367 5745
Fragments arranged by size:
5745 606 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2546 2567 2584 4039 5494
Size: 325 217 627 759 21 17 1455 1455
Cuts at: 5494 618
Size: 1842
Fragments arranged by size:
1842 1455 1455 759 627 325 217 21
17
BanII G_rGCy'C
Cuts at: 1391 2157 3120 3369 4037 1391
Size: 766 963 249 668 4072
Fragments arranged by size:
4072 963 766 668 249
BbeI G_GCGC'C
Cuts at: 2588 2588
Size: 6718
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5747
BbvCI CC'TCA_GC
Cuts at: 2531 2531
Size: 6718
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4909 4943 1903
Size: 34 50 34 2888 34 3678
Fragments arranged by size:
3678 2888 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4610 5820 4610
Size: 1210 5508
BclI T'GATC_A
Cuts at: 1548 2130 1548
Size: 582 6136
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3364 5329 136
Size: 107 122 71 631 605 1692 1965 1525
Fragments arranged by size:
1965 1692 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6713 6713
Size: 6718
BlpI GC'TnA_GC
Cuts at: 4135 4135
Size: 6718
Bme1580I G_kGCm'C
Cuts at: 1790 2157 3354 3811 4779 5935 1790
Size: 367 1197 457 968 1156 2573
Fragments arranged by size:
2573 1197 1156 968 457 367
BmgBI CAC'GTC
Cuts at: 1318 2905 1318
Size: 1587 5131
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3509 3827 4449
Size: 416 53 83 399 24 2092 318 622
Cuts at: 4449 5407 442
Size: 958 1753
Fragments arranged by size:
2092 1753 958 622 416 399 318 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 3113 5378 1239
Size: 839 1035 2265 2579
Fragments arranged by size:
2579 2265 1035 839
Bpu10I CC'TnA_GC
Cuts at: 2531 2531
Size: 6718
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3366 4843 5708 6006 3366
Size: 1477 865 298 4078
Fragments arranged by size:
4078 1477 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3253 3518 5381 882
Size: 617 1754 265 1863 2219
Fragments arranged by size:
2219 1863 1754 617 265
BsaAI yAC'GTr
Cuts at: 493 1204 2311 4509 493
Size: 711 1107 2198 2702
Fragments arranged by size:
2702 2198 1107 711
BsaBI GATnn'nnATC
Cuts at: 6712 6712
Size: 6718
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2244 2585 2604 4525
Size: 53 83 186 936 711 341 19 1921
Cuts at: 4525 4907 5542 275
Size: 382 635 1451
Fragments arranged by size:
1921 1451 936 711 635 382 341 186
83 53 19
BsaWI w'CCGG_w
Cuts at: 2818 5151 5549 5823 5970 6381 2818
Size: 2333 398 274 147 411 3155
Fragments arranged by size:
3155 2333 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3062 3092 3924 3954 1178
Size: 30 165 30 1659 30 832 30 3942
Fragments arranged by size:
3942 1659 832 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 3083 3242 1186
Size: 1018 879 159 4662
Fragments arranged by size:
4662 1018 879 159
BseYI C'CCAG_C
Cuts at: 2805 3119 5921 6417 2805
Size: 314 2802 496 3106
Fragments arranged by size:
3106 2802 496 314
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2085 3017 3348 1163
Size: 573 349 932 331 4533
Fragments arranged by size:
4533 932 573 349 331
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2831 4168 4929 5957 1540
Size: 275 186 830 1337 761 1028 2301
Fragments arranged by size:
2301 1337 1028 830 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2172 2523 2595 3099 3354 4779 4864 5935 2172
Size: 351 72 504 255 1425 85 1071 2955
Fragments arranged by size:
2955 1425 1071 504 351 255 85 72
BsmI GAATG_Cn'
Cuts at: 2961 3618 4229 4322 2961
Size: 657 611 93 5357
Fragments arranged by size:
5357 657 611 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3232 3232
Size: 6718
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2172 2523 2595 3099 3120 3354
Size: 399 367 15 351 72 504 21 234
Cuts at: 3354 3369 3811 4037 4779 4864 5935 1391
Size: 15 442 226 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 504 442 399 367 351
234 226 85 72 21 15 15
BspEI T'CCGG_A
Cuts at: 2818 6381 2818
Size: 3563 3155
BspHI T'CATG_A
Cuts at: 2127 2133 2793 4607 6337 2127
Size: 6 660 1814 1730 2508
Fragments arranged by size:
2508 1814 1730 660 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3446 4073 844
Size: 2602 627 3489
Fragments arranged by size:
3489 2602 627
BsrBI CCG'CTC
Cuts at: 1394 1823 4605 1394
Size: 429 2782 3507
Fragments arranged by size:
3507 2782 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5213 5387 65
Size: 1183 3965 174 1396
Fragments arranged by size:
3965 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2997 5362 5549 1700
Size: 110 1187 2365 187 2869
Fragments arranged by size:
2869 2365 1187 187 110
BsrGI T'GTAC_A
Cuts at: 96 2145 2637 96
Size: 2049 492 4177
Fragments arranged by size:
4177 2049 492
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6718
BssSI C'ACGA_G
Cuts at: 4778 5790 4778
Size: 1012 5706
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6718
BstBI TT'CG_AA
Cuts at: 5522 5522
Size: 6718
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6718
BstXI CCAn_nnnn'nTGG
Cuts at: 3050 3164 3050
Size: 114 6604
BstYI r'GATC_y
Cuts at: 1972 3442 4048 4415 4800 4817 6269 6355 6367
Size: 1470 606 367 385 17 1452 86 12
Cuts at: 6367 6713 1972
Size: 346 1977
Fragments arranged by size:
1977 1470 1452 606 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3361 3361
Size: 6718
BtgI C'CryG_G
Cuts at: 513 1065 3936 513
Size: 552 2871 3295
Fragments arranged by size:
3295 2871 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5979
Fragments arranged by size:
5979 683 56
BtsI GCAGTG_nn'
Cuts at: 3029 3312 3354 4234 5028 5048 3029
Size: 283 42 880 794 20 4699
Fragments arranged by size:
4699 880 794 283 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6718
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6683
DraI TTT'AAA
Cuts at: 2916 4022 2916
Size: 1106 5612
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5725 809
Size: 507 4409 1802
Fragments arranged by size:
4409 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2828 4165 5054
Size: 54 1425 50 275 18 998 1337 889
Cuts at: 5054 6683 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1337 998 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2828 4165 1537
Size: 275 1016 1337 4090
Fragments arranged by size:
4090 1337 1016 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2511 2892 2903 2945 2970 3102 3696 4648
Size: 1158 381 11 42 25 132 594 952
Cuts at: 4648 1353
Size: 3423
Fragments arranged by size:
3423 1158 952 594 381 132 42 25
11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5305 5679 5825 387
Size: 1064 138 3716 374 146 1280
Fragments arranged by size:
3716 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2441 3113 4781 5378 6165 6433
Size: 393 446 363 672 1668 597 787 268
Cuts at: 6433 1239
Size: 1524
Fragments arranged by size:
1668 1524 787 672 597 446 393 363
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2186 2186
Size: 6718
EcoO109I rG'GnC_Cy
Cuts at: 2153 2179 2416 3059 3458 2153
Size: 26 237 643 399 5413
Fragments arranged by size:
5413 643 399 237 26
EcoRI G'AATT_C
Cuts at: 4027 4405 4027
Size: 378 6340
FspI TGC'GCA
Cuts at: 5224 5224
Size: 6718
HaeII r_GCGC'y
Cuts at: 1678 2588 3962 5865 1678
Size: 910 1374 1903 2531
Fragments arranged by size:
2531 1903 1374 910
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2115 2147 2317 2349 2769
Size: 32 135 32 543 32 170 32 420
Cuts at: 2769 2801 3231 3263 3517 3549 5359 5391 5433
Size: 32 430 32 254 32 1810 32 42
Cuts at: 5433 5465 1373
Size: 32 2626
Fragments arranged by size:
2626 1810 543 430 420 254 170 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2608 4062 4308 669
Size: 988 281 670 1454 246 3079
Fragments arranged by size:
3079 1454 988 670 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6718
HpaI GTT'AAC
Cuts at: 4308 4308
Size: 6718
KasI G'GCGC_C
Cuts at: 2584 2584
Size: 6718
KpnI G_GTAC'C
Cuts at: 4043 4043
Size: 6718
MfeI C'AATT_G
Cuts at: 4317 4317
Size: 6718
MluI A'CGCG_T
Cuts at: 6594 6594
Size: 6718
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2610 5832 6016 1305
Size: 1305 3222 184 2007
Fragments arranged by size:
3222 2007 1305 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4896
Fragments arranged by size:
4896 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 2132 2598 3162 3342 3858 4676
Size: 648 870 96 466 564 180 516 818
Cuts at: 4676 5035 5194 518
Size: 359 159 2042
Fragments arranged by size:
2042 870 818 648 564 516 466 359
180 159 96
MspA1I CmG'CkG
Cuts at: 1606 2079 3710 4134 4811 5959 6204 1606
Size: 473 1631 424 677 1148 245 2120
Fragments arranged by size:
2120 1631 1148 677 473 424 245
NarI GG'CG_CC
Cuts at: 2585 2585
Size: 6718
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6166
NdeI CA'TA_TG
Cuts at: 387 2686 3492 387
Size: 2299 806 3613
Fragments arranged by size:
3613 2299 806
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6718
NotI GC'GGCC_GC
Cuts at: 4165 4165
Size: 6718
NspI r_CATG'y
Cuts at: 1132 1229 2020 4076 5621 1132
Size: 97 791 2056 1545 2229
Fragments arranged by size:
2229 2056 1545 791 97
PasI CC'CwG_GG
Cuts at: 1348 2119 3043 1348
Size: 771 924 5023
Fragments arranged by size:
5023 924 771
PciI A'CATG_T
Cuts at: 2016 5617 2016
Size: 3601 3117
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3468 1108
Size: 2360 4358
PfoI T'CCnGG_A
Cuts at: 2055 3445 3974 2055
Size: 1390 529 4799
Fragments arranged by size:
4799 1390 529
PmeI GTTT'AAAC
Cuts at: 4022 4022
Size: 6718
PmlI CAC'GTG
Cuts at: 2311 2311
Size: 6718
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4787 4819 6300 6332 4787
Size: 32 1481 32 5173
Fragments arranged by size:
5173 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2416 3059 3458 2416
Size: 643 399 5676
Fragments arranged by size:
5676 643 399
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6718
PsiI TTA'TAA
Cuts at: 4288 4288
Size: 6718
PspOMI G'GGCC_C
Cuts at: 2153 2153
Size: 6718
PstI C_TGCA'G
Cuts at: 830 3129 4070 830
Size: 2299 941 3478
Fragments arranged by size:
3478 2299 941
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6718
PvuII CAG'CTG
Cuts at: 3710 3710
Size: 6718
SalI G'TCGA_C
Cuts at: 1936 4060 1936
Size: 2124 4594
SapI GCTCTTCn'nnn_
Cuts at: 2511 3696 2511
Size: 1185 5533
SbfI CC_TGCA'GG
Cuts at: 4070 4070
Size: 6718
ScaI AGT'ACT
Cuts at: 2267 2332 2655 2768 3387 3613 4966 6678 2267
Size: 65 323 113 619 226 1353 1712 2307
Fragments arranged by size:
2307 1712 1353 619 323 226 113 65
SfcI C'TryA_G
Cuts at: 826 1045 2649 3125 4066 5201 5882 6073 826
Size: 219 1604 476 941 1135 681 191 1471
Fragments arranged by size:
1604 1471 1135 941 681 476 219 191
SfoI GGC'GCC
Cuts at: 2586 2586
Size: 6718
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6718
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6718
SmaI CCC'GGG
Cuts at: 1872 4045 1872
Size: 2173 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3381 4822 5723 5985 820
Size: 197 926 1438 1441 901 262 1553
Fragments arranged by size:
1553 1441 1438 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4509 493
Size: 4016 2702
SpeI A'CTAG_T
Cuts at: 152 3743 152
Size: 3591 3127
SphI G_CATG'C
Cuts at: 1132 4076 1132
Size: 2944 3774
SspI AAT'ATT
Cuts at: 5 52 4642 5
Size: 47 4590 2081
Fragments arranged by size:
4590 2081 47
StuI AGG'CCT
Cuts at: 1517 2863 1517
Size: 1346 5372
StyI C'CwwG_G
Cuts at: 513 1065 4157 513
Size: 552 3092 3074
Fragments arranged by size:
3092 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3114 3562 3898 4745 4762 4915 5100
Size: 1255 1534 448 336 847 17 153 185
Cuts at: 5100 5545 325
Size: 445 1498
Fragments arranged by size:
1534 1498 1255 847 448 445 336 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 2265 2283
Size: 274 80 33 51 1443 168 120 18
Cuts at: 2283 2330 2637 2653 2766 3385 3611 4964 6676
Size: 47 307 16 113 619 226 1353 1712
Cuts at: 6676 96
Size: 138
Fragments arranged by size:
1712 1443 1353 619 307 274 226 168
138 120 113 80 51 47 33 18
16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2388 2420 3790 3822 3924 3956 3988 6678 6710
Size: 32 1370 32 102 32 32 2690 32
Cuts at: 6710 2388
Size: 2396
Fragments arranged by size:
2690 2396 1370 102 32 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6718
XbaI T'CTAG_A
Cuts at: 4054 4054
Size: 6718
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2053 3208 2053
Size: 1155 5563
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6718
XmaI C'CCGG_G
Cuts at: 1870 4043 1870
Size: 2173 4545
XmnI GAAnn'nnTTC
Cuts at: 4847 4847
Size: 6718
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2605 4526 276
Size: 53 83 186 936 1071 1921 2468
Fragments arranged by size:
2468 1921 1071 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SrfI SwaI