Restriction map for
pFN21AE2037
(Circular) (Six-base) MAPSORT of: pFN21AE2037.seq Check: 7578 from: 1 to: 6607
pFN21AE2037 6607 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3029 3029
Size: 6607
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4417 278
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3705 3950 1937
Size: 1768 245 4594
Fragments arranged by size:
4594 1768 245
Acc65I G'GTAC_C
Cuts at: 3928 3928
Size: 6607
AclI AA'CG_TT
Cuts at: 1563 4734 5107 1563
Size: 3171 373 3063
Fragments arranged by size:
3171 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2173 4670 6054 6322 1632
Size: 541 2497 1384 268 1917
Fragments arranged by size:
2497 1917 1384 541 268
AflII C'TTAA_G
Cuts at: 820 1017 3778 3844 820
Size: 197 2761 66 3583
Fragments arranged by size:
3583 2761 197 66
AflIII A'CryG_T
Cuts at: 1203 5506 6483 1203
Size: 4303 977 1327
Fragments arranged by size:
4303 1327 977
AgeI A'CCGG_T
Cuts at: 5438 5438
Size: 6607
AhdI GACnn_n'nnGTC
Cuts at: 5336 5336
Size: 6607
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6607
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2676 1184
Size: 1492 5115
AlwNI CAG_nnn'CTG
Cuts at: 4350 5922 4350
Size: 1572 5035
ApaI G_GGCC'C
Cuts at: 2123 2123
Size: 6607
ApaLI G'TGCA_C
Cuts at: 4664 5820 4664
Size: 1156 5451
ApoI r'AATT_y
Cuts at: 1493 2395 3916 4142 4294 6403 1493
Size: 902 1521 226 152 2109 1697
Fragments arranged by size:
2109 1697 1521 902 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3066 5161 160
Size: 872 2034 2095 1606
Fragments arranged by size:
2095 2034 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
AvaI C'yCGr_G
Cuts at: 1870 1943 2111 3932 1870
Size: 73 168 1821 4545
Fragments arranged by size:
4545 1821 168 73
BamHI G'GATC_C
Cuts at: 2008 3937 4304 2008
Size: 1929 367 4311
Fragments arranged by size:
4311 1929 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2074 2268 2300 2347 2924
Size: 325 217 627 287 194 32 47 577
Cuts at: 2924 3928 5383 618
Size: 1004 1455 1842
Fragments arranged by size:
1842 1455 1004 627 577 325 287 217
194 47 32
BanII G_rGCy'C
Cuts at: 1391 2123 3926 1391
Size: 732 1803 4072
Fragments arranged by size:
4072 1803 732
BbeI G_GCGC'C
Cuts at: 2078 2304 2078
Size: 226 6381
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5636
BbvCI CC'TCA_GC
Cuts at: 2177 2177
Size: 6607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4798 4832 1903
Size: 34 2861 34 3678
Fragments arranged by size:
3678 2861 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4499 5709 4499
Size: 1210 5397
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6607
BfrBI ATG'CAT
Cuts at: 3768 3768
Size: 6607
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2127 5218 136
Size: 107 122 71 631 605 455 3091 1525
Fragments arranged by size:
3091 1525 631 605 455 122 107 71
BglII A'GATC_T
Cuts at: 6602 6602
Size: 6607
BlpI GC'TnA_GC
Cuts at: 4024 4024
Size: 6607
Bme1580I G_kGCm'C
Cuts at: 1790 2123 2229 2273 2551 2655 3353 4668 5824
Size: 333 106 44 278 104 698 1315 1156
Cuts at: 5824 1790
Size: 2573
Fragments arranged by size:
2573 1315 1156 698 333 278 106 104
44
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6607
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4338 5296 442
Size: 416 53 83 399 24 2921 958 1753
Fragments arranged by size:
2921 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6607
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3230 3230
Size: 6607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2442 3225 5267 1239
Size: 1203 783 2042 2579
Fragments arranged by size:
2579 2042 1203 783
Bpu10I CC'TnA_GC
Cuts at: 2177 3193 2177
Size: 1016 5591
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2349 2916 3824 4732 5597 5895 2349
Size: 567 908 908 865 298 3061
Fragments arranged by size:
3061 908 908 865 567 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3800 5270 882
Size: 617 2301 1470 2219
Fragments arranged by size:
2301 2219 1470 617
BsaAI yAC'GTr
Cuts at: 493 1204 2656 4398 493
Size: 711 1452 1742 2702
Fragments arranged by size:
2702 1742 1452 711
BsaBI GATnn'nnATC
Cuts at: 6601 6601
Size: 6607
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2075 2301 4414 4796
Size: 53 83 186 936 542 226 2113 382
Cuts at: 4796 5431 275
Size: 635 1451
Fragments arranged by size:
2113 1451 936 635 542 382 226 186
83 53
BsaWI w'CCGG_w
Cuts at: 5040 5438 5712 5859 6270 5040
Size: 398 274 147 411 5377
Fragments arranged by size:
5377 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6382
Fragments arranged by size:
6382 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2579 3539 3686 1186
Size: 1393 960 147 4107
Fragments arranged by size:
4107 1393 960 147
BseYI C'CCAG_C
Cuts at: 2085 2163 2290 5810 6306 2085
Size: 78 127 3520 496 2386
Fragments arranged by size:
3520 2386 496 127 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6034
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2157 4057 4818 5846 1540
Size: 275 186 156 1900 761 1028 2301
Fragments arranged by size:
2301 1900 1028 761 275 186 156
BsiHKAI G_wGCw'C
Cuts at: 2478 2617 3615 4668 4753 5824 2478
Size: 139 998 1053 85 1071 3261
Fragments arranged by size:
3261 1071 1053 998 139 85
BsmI GAATG_Cn'
Cuts at: 4118 4211 4118
Size: 93 6514
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2229 2273 2478 2551 2617 2655
Size: 399 333 106 44 205 73 66 38
Cuts at: 2655 3353 3615 3926 4668 4753 5824 1391
Size: 698 262 311 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 698 399 333 311 262
205 106 85 73 66 44 38
BspEI T'CCGG_A
Cuts at: 6270 6270
Size: 6607
BspHI T'CATG_A
Cuts at: 4496 6226 4496
Size: 1730 4877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3029 3962 844
Size: 2185 933 3489
Fragments arranged by size:
3489 2185 933
BsrBI CCG'CTC
Cuts at: 1394 1823 4494 1394
Size: 429 2671 3507
Fragments arranged by size:
3507 2671 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3027 3106 3768 3864 5102 5276 65
Size: 1183 1779 79 662 96 1238 174 1396
Fragments arranged by size:
1779 1396 1238 1183 662 174 96 79
BsrFI r'CCGG_y
Cuts at: 1700 1810 2193 2321 5251 5438 1700
Size: 110 383 128 2930 187 2869
Fragments arranged by size:
2930 2869 383 187 128 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6607
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6607
BssSI C'ACGA_G
Cuts at: 4667 5679 4667
Size: 1012 5595
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6607
BstBI TT'CG_AA
Cuts at: 5411 5411
Size: 6607
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6607
BstYI r'GATC_y
Cuts at: 1972 2008 3380 3937 4304 4689 4706 6158 6244
Size: 36 1372 557 367 385 17 1452 86
Cuts at: 6244 6256 6602 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1372 557 385 367 346 86
36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2314 513
Size: 552 939 310 4806
Fragments arranged by size:
4806 939 552 310
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5868
Fragments arranged by size:
5868 683 56
BtsI GCAGTG_nn'
Cuts at: 2023 4123 4917 4937 2023
Size: 2100 794 20 3693
Fragments arranged by size:
3693 2100 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6607
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6572
DraI TTT'AAA
Cuts at: 3814 3911 3814
Size: 97 6510
DraIII CAC_nnn'GTG
Cuts at: 3430 3430
Size: 6607
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2452 5614 809
Size: 507 1136 3162 1802
Fragments arranged by size:
3162 1802 1136 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2154 2890 4054
Size: 54 1425 50 275 18 324 736 1164
Cuts at: 4054 4943 6572 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1164 889 736 324 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2154 4054 1537
Size: 275 342 1900 4090
Fragments arranged by size:
4090 1900 342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2565 3516 4537 1353
Size: 1212 951 1021 3423
Fragments arranged by size:
3423 1212 1021 951
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2111 5194 5568 5714 387
Size: 1064 138 522 3083 374 146 1280
Fragments arranged by size:
3083 1280 1064 522 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2173 2442 3225 4670 5267 6054 6322
Size: 393 541 269 783 1445 597 787 268
Cuts at: 6322 1239
Size: 1524
Fragments arranged by size:
1524 1445 787 783 597 541 393 269
268
EcoO109I rG'GnC_Cy
Cuts at: 2120 2120
Size: 6607
EcoRI G'AATT_C
Cuts at: 3916 4294 3916
Size: 378 6229
FspI TGC'GCA
Cuts at: 5113 5113
Size: 6607
HaeII r_GCGC'y
Cuts at: 1678 2078 2282 2304 5754 1678
Size: 400 204 22 3450 2531
Fragments arranged by size:
3450 2531 400 204 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2360 2392 3064 3096 3602
Size: 32 135 32 788 32 672 32 506
Cuts at: 3602 3634 3698 3730 5248 5280 5322 5354 1373
Size: 32 64 32 1518 32 42 32 2626
Fragments arranged by size:
2626 1518 788 672 506 135 64 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2209 2673 2713 3951 4197 669
Size: 988 281 271 464 40 1238 246 3079
Fragments arranged by size:
3079 1238 988 464 281 271 246 40
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6607
HpaI GTT'AAC
Cuts at: 4197 4197
Size: 6607
KasI G'GCGC_C
Cuts at: 2074 2300 2074
Size: 226 6381
KpnI G_GTAC'C
Cuts at: 3932 3932
Size: 6607
MfeI C'AATT_G
Cuts at: 3324 3870 4206 3324
Size: 546 336 5725
Fragments arranged by size:
5725 546 336
MluI A'CGCG_T
Cuts at: 6483 6483
Size: 6607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3107 3207 5721 5905 1305
Size: 1802 100 2514 184 2007
Fragments arranged by size:
2514 2007 1802 184 100
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2892 10
Size: 54 1425 343 1060 3725
Fragments arranged by size:
3725 1425 1060 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2634 2658 4565 4924 5083 518
Size: 648 1468 24 1907 359 159 2042
Fragments arranged by size:
2042 1907 1468 648 359 159 24
MspA1I CmG'CkG
Cuts at: 1606 2052 2167 2294 2328 2402 4023 4700 5848
Size: 446 115 127 34 74 1621 677 1148
Cuts at: 5848 6093 1606
Size: 245 2120
Fragments arranged by size:
2120 1621 1148 677 446 245 127 115
74 34
NaeI GCC'GGC
Cuts at: 2195 2323 2195
Size: 128 6479
NarI GG'CG_CC
Cuts at: 2075 2301 2075
Size: 226 6381
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5116
Fragments arranged by size:
5116 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6607
NgoMIV G'CCGG_C
Cuts at: 2193 2321 2193
Size: 128 6479
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6607
NotI GC'GGCC_GC
Cuts at: 4054 4054
Size: 6607
NsiI A_TGCA'T
Cuts at: 3770 3770
Size: 6607
NspI r_CATG'y
Cuts at: 1132 1229 2633 3020 3965 5510 1132
Size: 97 1404 387 945 1545 2229
Fragments arranged by size:
2229 1545 1404 945 387 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6607
PciI A'CATG_T
Cuts at: 5506 5506
Size: 6607
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2510 1108
Size: 1402 5205
PmeI GTTT'AAAC
Cuts at: 3911 3911
Size: 6607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4676 4708 6189 6221 4676
Size: 32 1481 32 5062
Fragments arranged by size:
5062 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6607
PsiI TTA'TAA
Cuts at: 4177 4177
Size: 6607
PspOMI G'GGCC_C
Cuts at: 2119 2119
Size: 6607
PstI C_TGCA'G
Cuts at: 830 3959 830
Size: 3129 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6607
PvuII CAG'CTG
Cuts at: 2294 2402 2294
Size: 108 6499
SalI G'TCGA_C
Cuts at: 1936 3949 1936
Size: 2013 4594
SbfI CC_TGCA'GG
Cuts at: 3959 3959
Size: 6607
ScaI AGT'ACT
Cuts at: 4855 6567 4855
Size: 1712 4895
SfcI C'TryA_G
Cuts at: 826 1045 3955 5090 5771 5962 826
Size: 219 2910 1135 681 191 1471
Fragments arranged by size:
2910 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2076 2302 2076
Size: 226 6381
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6607
SmaI CCC'GGG
Cuts at: 1872 2113 3934 1872
Size: 241 1821 4545
Fragments arranged by size:
4545 1821 241
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2364 2931 3778 3803 3844 4711
Size: 197 926 421 567 847 25 41 867
Cuts at: 4711 5612 5874 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 867 847 567 421 262
197 41 25
SnaBI TAC'GTA
Cuts at: 493 4398 493
Size: 3905 2702
SpeI A'CTAG_T
Cuts at: 152 3130 152
Size: 2978 3629
SphI G_CATG'C
Cuts at: 1132 3965 1132
Size: 2833 3774
SspI AAT'ATT
Cuts at: 5 52 4531 5
Size: 47 4479 2081
Fragments arranged by size:
4479 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6607
StyI C'CwwG_G
Cuts at: 513 1065 2004 2517 3239 4046 513
Size: 552 939 513 722 807 3074
Fragments arranged by size:
3074 939 807 722 552 513
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4634 4651 4804 4989 5434 325
Size: 1255 3054 17 153 185 445 1498
Fragments arranged by size:
3054 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3336 4853 6565
Size: 274 80 33 51 1443 1359 1517 1712
Cuts at: 6565 96
Size: 138
Fragments arranged by size:
1712 1517 1443 1359 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6567 6599 6567
Size: 32 6575
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6607
XbaI T'CTAG_A
Cuts at: 2878 3943 2878
Size: 1065 5542
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2466 3090 2466
Size: 624 5983
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6607
XmaI C'CCGG_G
Cuts at: 1870 2111 3932 1870
Size: 241 1821 4545
Fragments arranged by size:
4545 1821 241
XmnI GAAnn'nnTTC
Cuts at: 2867 3448 4736 2867
Size: 581 1288 4738
Fragments arranged by size:
4738 1288 581
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4415 276
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsiWI BsmBI BstXI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI