Restriction map for
pFN21AE2035
(Circular) (Six-base) MAPSORT of: pFN21AE2035.seq Check: 6834 from: 1 to: 6592
pFN21AE2035 6592 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4402 278
Size: 53 83 186 936 2866 2468
Fragments arranged by size:
2866 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3935 1937
Size: 1998 4594
Acc65I G'GTAC_C
Cuts at: 3913 3913
Size: 6592
AclI AA'CG_TT
Cuts at: 1563 4719 5092 1563
Size: 3156 373 3063
Fragments arranged by size:
3156 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2041 4655 6039 6307 1632
Size: 409 2614 1384 268 1917
Fragments arranged by size:
2614 1917 1384 409 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6395
AflIII A'CryG_T
Cuts at: 1203 2485 5491 6468 1203
Size: 1282 3006 977 1327
Fragments arranged by size:
3006 1327 1282 977
AgeI A'CCGG_T
Cuts at: 5423 5423
Size: 6592
AhdI GACnn_n'nnGTC
Cuts at: 5321 5321
Size: 6592
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6592
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6592
AlwNI CAG_nnn'CTG
Cuts at: 3769 4335 5907 3769
Size: 566 1572 4454
Fragments arranged by size:
4454 1572 566
ApaLI G'TGCA_C
Cuts at: 4649 5805 4649
Size: 1156 5436
ApoI r'AATT_y
Cuts at: 1493 2637 2810 2846 3001 3465 3472 3616 3782
Size: 1144 173 36 155 464 7 144 166
Cuts at: 3782 3901 4127 4279 6388 1493
Size: 119 226 152 2109 1697
Fragments arranged by size:
2109 1697 1144 464 226 173 166 155
152 144 119 36 7
AseI AT'TA_AT
Cuts at: 160 1032 2244 3828 5146 160
Size: 872 1212 1584 1318 1606
Fragments arranged by size:
1606 1584 1318 1212 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6592
AvaI C'yCGr_G
Cuts at: 1870 1943 3917 1870
Size: 73 1974 4545
Fragments arranged by size:
4545 1974 73
BamHI G'GATC_C
Cuts at: 3922 4289 3922
Size: 367 6225
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3036 3913 5368 618
Size: 325 217 627 1249 877 1455 1842
Fragments arranged by size:
1842 1455 1249 877 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3911 1391
Size: 2520 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5621
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4783 4817 1903
Size: 34 2846 34 3678
Fragments arranged by size:
3678 2846 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4484 5694 4484
Size: 1210 5382
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6592
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5203 136
Size: 107 122 71 631 605 3531 1525
Fragments arranged by size:
3531 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6587 6587
Size: 6592
BlpI GC'TnA_GC
Cuts at: 4009 4009
Size: 6592
Bme1580I G_kGCm'C
Cuts at: 1790 2712 3039 4653 5809 1790
Size: 922 327 1614 1156 2573
Fragments arranged by size:
2573 1614 1156 922 327
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6592
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3159 3738 4323
Size: 416 53 83 399 24 1742 579 585
Cuts at: 4323 5281 442
Size: 958 1753
Fragments arranged by size:
1753 1742 958 585 579 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6592
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3398 5252 1239
Size: 2159 1854 2579
Fragments arranged by size:
2579 2159 1854
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2515 4717 5582 5880 2515
Size: 2202 865 298 3227
Fragments arranged by size:
3227 2202 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5255 882
Size: 617 3756 2219
Fragments arranged by size:
3756 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2479 2875 4383 493
Size: 711 1275 396 1508 2702
Fragments arranged by size:
2702 1508 1275 711 396
BsaBI GATnn'nnATC
Cuts at: 6586 6586
Size: 6592
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4399 4781 5416 275
Size: 53 83 186 936 2866 382 635 1451
Fragments arranged by size:
2866 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5025 5423 5697 5844 6255 5025
Size: 398 274 147 411 5362
Fragments arranged by size:
5362 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6367
Fragments arranged by size:
6367 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3072 3722 3827 1186
Size: 1886 650 105 3951
Fragments arranged by size:
3951 1886 650 105
BseYI C'CCAG_C
Cuts at: 2447 5795 6291 2447
Size: 3348 496 2748
Fragments arranged by size:
3348 2748 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2290 1163
Size: 573 554 5465
Fragments arranged by size:
5465 573 554
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4042 4803 5831 1540
Size: 275 186 2041 761 1028 2301
Fragments arranged by size:
2301 2041 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4653 4738 5809 4653
Size: 85 1071 5436
Fragments arranged by size:
5436 1071 85
BsmI GAATG_Cn'
Cuts at: 2449 2981 3680 4103 4196 2449
Size: 532 699 423 93 4845
Fragments arranged by size:
4845 699 532 423 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2712 3039 3911 4653 4738 5809 1391
Size: 399 922 327 872 742 85 1071 2174
Fragments arranged by size:
2174 1071 922 872 742 399 327 85
BspEI T'CCGG_A
Cuts at: 6255 6255
Size: 6592
BspHI T'CATG_A
Cuts at: 4481 6211 4481
Size: 1730 4862
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3271 3947 844
Size: 2427 676 3489
Fragments arranged by size:
3489 2427 676
BsrBI CCG'CTC
Cuts at: 1394 1823 4479 1394
Size: 429 2656 3507
Fragments arranged by size:
3507 2656 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3229 5087 5261 65
Size: 1183 1981 1858 174 1396
Fragments arranged by size:
1981 1858 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5236 5423 1700
Size: 110 3426 187 2869
Fragments arranged by size:
3426 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6592
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6592
BssSI C'ACGA_G
Cuts at: 4652 5664 4652
Size: 1012 5580
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6592
BstBI TT'CG_AA
Cuts at: 5396 5396
Size: 6592
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6592
BstXI CCAn_nnnn'nTGG
Cuts at: 3727 3727
Size: 6592
BstYI r'GATC_y
Cuts at: 1972 3165 3922 4289 4674 4691 6143 6229 6241
Size: 1193 757 367 385 17 1452 86 12
Cuts at: 6241 6587 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1193 757 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3483 3483
Size: 6592
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6040
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5853
Fragments arranged by size:
5853 683 56
BtsI GCAGTG_nn'
Cuts at: 2307 4108 4902 4922 2307
Size: 1801 794 20 3977
Fragments arranged by size:
3977 1801 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6592
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2460 2495 529
Size: 35 1896 35 4626
Fragments arranged by size:
4626 1896 35 35
DraI TTT'AAA
Cuts at: 3896 3896
Size: 6592
DraIII CAC_nnn'GTG
Cuts at: 3746 3746
Size: 6592
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5599 809
Size: 507 4283 1802
Fragments arranged by size:
4283 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 4039 4928
Size: 54 1425 50 275 18 268 1941 889
Cuts at: 4928 6557 8
Size: 1629 43
Fragments arranged by size:
1941 1629 1425 889 275 268 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4039 1537
Size: 275 2227 4090
Fragments arranged by size:
4090 2227 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 2486 2505 2613 4522 1353
Size: 707 426 19 108 1909 3423
Fragments arranged by size:
3423 1909 707 426 108 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2364 5179 5553 5699 387
Size: 1064 138 775 2815 374 146 1280
Fragments arranged by size:
2815 1280 1064 775 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 3398 4655 5252 6039 6307 1239
Size: 393 409 1357 1257 597 787 268 1524
Fragments arranged by size:
1524 1357 1257 787 597 409 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3283 3371 3283
Size: 88 6504
EcoRI G'AATT_C
Cuts at: 2846 3001 3616 3901 4279 2846
Size: 155 615 285 378 5159
Fragments arranged by size:
5159 615 378 285 155
EcoRV GAT'ATC
Cuts at: 2372 2372
Size: 6592
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 6592
FspI TGC'GCA
Cuts at: 5098 5098
Size: 6592
HaeII r_GCGC'y
Cuts at: 1678 5739 1678
Size: 4061 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2217 2249 3806 3838 5233
Size: 32 135 32 645 32 1557 32 1395
Cuts at: 5233 5265 5307 5339 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1557 1395 645 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3936 4182 669
Size: 988 281 1998 246 3079
Fragments arranged by size:
3079 1998 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6592
HpaI GTT'AAC
Cuts at: 4182 4182
Size: 6592
KpnI G_GTAC'C
Cuts at: 3917 3917
Size: 6592
MfeI C'AATT_G
Cuts at: 2745 4191 2745
Size: 1446 5146
MluI A'CGCG_T
Cuts at: 6468 6468
Size: 6592
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3010 5706 5890 1305
Size: 1705 2696 184 2007
Fragments arranged by size:
2696 2007 1705 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2100 10
Size: 54 1425 343 268 4502
Fragments arranged by size:
4502 1425 343 268 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2344 2646 4550 4909 5068 518
Size: 648 1178 302 1904 359 159 2042
Fragments arranged by size:
2042 1904 1178 648 359 302 159
MspA1I CmG'CkG
Cuts at: 1606 2061 4008 4685 5833 6078 1606
Size: 455 1947 677 1148 245 2120
Fragments arranged by size:
2120 1947 1148 677 455 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6040
NdeI CA'TA_TG
Cuts at: 387 2830 387
Size: 2443 4149
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6592
NotI GC'GGCC_GC
Cuts at: 4039 4039
Size: 6592
NspI r_CATG'y
Cuts at: 1132 1229 2294 2489 3950 5495 1132
Size: 97 1065 195 1461 1545 2229
Fragments arranged by size:
2229 1545 1461 1065 195 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6592
PciI A'CATG_T
Cuts at: 2485 5491 2485
Size: 3006 3586
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6592
PmeI GTTT'AAAC
Cuts at: 3896 3896
Size: 6592
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4661 4693 6174 6206 4661
Size: 32 1481 32 5047
Fragments arranged by size:
5047 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3283 3371 3283
Size: 88 6504
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6592
PsiI TTA'TAA
Cuts at: 2205 4162 2205
Size: 1957 4635
PstI C_TGCA'G
Cuts at: 830 2671 3944 830
Size: 1841 1273 3478
Fragments arranged by size:
3478 1841 1273
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6592
PvuII CAG'CTG
Cuts at: 2061 2061
Size: 6592
SalI G'TCGA_C
Cuts at: 1936 3934 1936
Size: 1998 4594
SapI GCTCTTCn'nnn_
Cuts at: 2505 2505
Size: 6592
SbfI CC_TGCA'GG
Cuts at: 3944 3944
Size: 6592
ScaI AGT'ACT
Cuts at: 2579 4840 6552 2579
Size: 2261 1712 2619
Fragments arranged by size:
2619 2261 1712
SfcI C'TryA_G
Cuts at: 826 1045 2667 2703 3030 3239 3345 3420 3940
Size: 219 1622 36 327 209 106 75 520
Cuts at: 3940 5075 5756 5947 826
Size: 1135 681 191 1471
Fragments arranged by size:
1622 1471 1135 681 520 327 219 209
191 106 75 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6592
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6592
SmaI CCC'GGG
Cuts at: 1872 3919 1872
Size: 2047 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2530 4696 5597 5859 820
Size: 197 926 587 2166 901 262 1553
Fragments arranged by size:
2166 1553 926 901 587 262 197
SnaBI TAC'GTA
Cuts at: 493 2875 4383 493
Size: 2382 1508 2702
Fragments arranged by size:
2702 2382 1508
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6592
SphI G_CATG'C
Cuts at: 1132 3950 1132
Size: 2818 3774
SspI AAT'ATT
Cuts at: 5 52 2417 3636 3850 4516 5
Size: 47 2365 1219 214 666 2081
Fragments arranged by size:
2365 2081 1219 666 214 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6592
StyI C'CwwG_G
Cuts at: 513 1065 2186 2921 4031 513
Size: 552 1121 735 1110 3074
Fragments arranged by size:
3074 1121 1110 735 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3757 4619 4636 4789 4974 5419 325
Size: 1255 2177 862 17 153 185 445 1498
Fragments arranged by size:
2177 1498 1255 862 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2301 2577 4838
Size: 274 80 33 51 1443 324 276 2261
Cuts at: 4838 6550 96
Size: 1712 138
Fragments arranged by size:
2261 1712 1443 324 276 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6552 6584 6552
Size: 32 6560
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6592
XbaI T'CTAG_A
Cuts at: 3928 3928
Size: 6592
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3724 3724
Size: 6592
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6592
XmaI C'CCGG_G
Cuts at: 1870 3917 1870
Size: 2047 4545
XmnI GAAnn'nnTTC
Cuts at: 2226 2958 3201 3753 4721 2226
Size: 732 243 552 968 4097
Fragments arranged by size:
4097 968 732 552 243
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4400 276
Size: 53 83 186 936 2866 2468
Fragments arranged by size:
2866 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI