Restriction map for
pFN21AE2031
(Circular) (Six-base) MAPSORT of: pFN21AE2031.seq Check: 4162 from: 1 to: 6568
pFN21AE2031 6568 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3424 3515 3802 3424
Size: 91 287 6190
Fragments arranged by size:
6190 287 91
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4378 278
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3093 3911 1937
Size: 1156 818 4594
Fragments arranged by size:
4594 1156 818
Acc65I G'GTAC_C
Cuts at: 3889 3889
Size: 6568
AclI AA'CG_TT
Cuts at: 1563 4695 5068 1563
Size: 3132 373 3063
Fragments arranged by size:
3132 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2945 3765 4631 6015 6283 1632
Size: 1313 820 866 1384 268 1917
Fragments arranged by size:
1917 1384 1313 866 820 268
AfeI AGC'GCT
Cuts at: 3556 3556
Size: 6568
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6371
AflIII A'CryG_T
Cuts at: 1203 5467 6444 1203
Size: 4264 977 1327
Fragments arranged by size:
4264 1327 977
AgeI A'CCGG_T
Cuts at: 5399 5399
Size: 6568
AhdI GACnn_n'nnGTC
Cuts at: 5297 5297
Size: 6568
AleI CACnn'nnGTG
Cuts at: 1166 2273 3857 1166
Size: 1107 1584 3877
Fragments arranged by size:
3877 1584 1107
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6568
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3227 3259 3227
Size: 32 6536
AlwNI CAG_nnn'CTG
Cuts at: 2545 2819 3724 4311 5883 2545
Size: 274 905 587 1572 3230
Fragments arranged by size:
3230 1572 905 587 274
ApaI G_GGCC'C
Cuts at: 2245 2245
Size: 6568
ApaLI G'TGCA_C
Cuts at: 3393 3818 4625 5781 3393
Size: 425 807 1156 4180
Fragments arranged by size:
4180 1156 807 425
ApoI r'AATT_y
Cuts at: 1493 2432 3877 4103 4255 6364 1493
Size: 939 1445 226 152 2109 1697
Fragments arranged by size:
2109 1697 1445 939 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5122 160
Size: 872 4090 1606
Fragments arranged by size:
4090 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
AvaI C'yCGr_G
Cuts at: 1870 1943 2835 3402 3893 1870
Size: 73 892 567 491 4545
Fragments arranged by size:
4545 892 567 491 73
BamHI G'GATC_C
Cuts at: 2535 3579 3898 4265 2535
Size: 1044 319 367 4838
Fragments arranged by size:
4838 1044 367 319
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3889 5344 618
Size: 325 217 627 2102 1455 1842
Fragments arranged by size:
2102 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2245 2511 2748 3045 3269 3887 1391
Size: 854 266 237 297 224 618 4072
Fragments arranged by size:
4072 854 618 297 266 237 224
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2718 3059 928
Size: 971 819 341 4437
Fragments arranged by size:
4437 971 819 341
BbvCI CC'TCA_GC
Cuts at: 2087 3281 2087
Size: 1194 5374
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4759 4793 1903
Size: 34 2822 34 3678
Fragments arranged by size:
3678 2822 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4460 5670 4460
Size: 1210 5358
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6568
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3848 5179 136
Size: 107 122 71 631 605 2176 1331 1525
Fragments arranged by size:
2176 1525 1331 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3238 6563 3238
Size: 3325 3243
BlpI GC'TnA_GC
Cuts at: 3558 3985 3558
Size: 427 6141
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2245 3397 3822 4629 5785 1790
Size: 228 227 1152 425 807 1156 2573
Fragments arranged by size:
2573 1156 1152 807 425 228 227
BmgBI CAC'GTC
Cuts at: 1318 2251 2725 3376 1318
Size: 933 474 651 4510
Fragments arranged by size:
4510 933 651 474
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4299 5257 442
Size: 416 53 83 399 24 2882 958 1753
Fragments arranged by size:
2882 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6568
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2318 2380 2891 3068 3197 3806 5228
Size: 986 93 62 511 177 129 609 1422
Cuts at: 5228 1239
Size: 2579
Fragments arranged by size:
2579 1422 986 609 511 177 129 93
62
Bpu10I CC'TnA_GC
Cuts at: 2087 3281 2087
Size: 1194 5374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4693 5558 5856 4693
Size: 865 298 5405
Fragments arranged by size:
5405 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2105 2177 2431 2834 5231 882
Size: 617 606 72 254 403 2397 2219
Fragments arranged by size:
2397 2219 617 606 403 254 72
BsaAI yAC'GTr
Cuts at: 493 1204 4359 493
Size: 711 3155 2702
Fragments arranged by size:
3155 2702 711
BsaBI GATnn'nnATC
Cuts at: 6562 6562
Size: 6568
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2322 2991 4375 4757
Size: 53 83 186 936 789 669 1384 382
Cuts at: 4757 5392 275
Size: 635 1451
Fragments arranged by size:
1451 1384 936 789 669 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5001 5399 5673 5820 6231 5001
Size: 398 274 147 411 5338
Fragments arranged by size:
5338 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2135 2165 2833 2863 1178
Size: 30 165 30 732 30 668 30 4883
Fragments arranged by size:
4883 732 668 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2489 3352 3701 1186
Size: 1303 863 349 4053
Fragments arranged by size:
4053 1303 863 349
BseYI C'CCAG_C
Cuts at: 3551 5771 6267 3551
Size: 2220 496 3852
Fragments arranged by size:
3852 2220 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2021 3107 3370 3527 3758 3776 3830
Size: 573 285 1086 263 157 231 18 54
Cuts at: 3830 1163
Size: 3901
Fragments arranged by size:
3901 1086 573 285 263 231 157 54
18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2274 2385 2697 4018 4779 5807
Size: 275 186 273 111 312 1321 761 1028
Cuts at: 5807 1540
Size: 2301
Fragments arranged by size:
2301 1321 1028 761 312 275 273 186
111
BsiHKAI G_wGCw'C
Cuts at: 3397 3822 4629 4714 5785 3397
Size: 425 807 85 1071 4180
Fragments arranged by size:
4180 1071 807 425 85
BsmI GAATG_Cn'
Cuts at: 4079 4172 4079
Size: 93 6475
BsmBI CGTCTCn'nnnn_
Cuts at: 3266 3665 3266
Size: 399 6169
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2245 2511 2748 3045 3269 3397
Size: 399 228 227 266 237 297 224 128
Cuts at: 3397 3822 3887 4629 4714 5785 1391
Size: 425 65 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 425 399 297 266 237
228 227 224 128 85 65
BspEI T'CCGG_A
Cuts at: 6231 6231
Size: 6568
BspHI T'CATG_A
Cuts at: 4457 6187 4457
Size: 1730 4838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2244 2670 3424 3515 3802 3923 844
Size: 1400 426 754 91 287 121 3489
Fragments arranged by size:
3489 1400 754 426 287 121 91
BsrBI CCG'CTC
Cuts at: 1394 1823 2264 3757 4455 1394
Size: 429 441 1493 698 3507
Fragments arranged by size:
3507 1493 698 441 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5063 5237 65
Size: 1183 3815 174 1396
Fragments arranged by size:
3815 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2217 2305 3653 5212 5399 1700
Size: 110 407 88 1348 1559 187 2869
Fragments arranged by size:
2869 1559 1348 407 187 110 88
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6568
BssHII G'CGCG_C
Cuts at: 1925 3706 1925
Size: 1781 4787
BssSI C'ACGA_G
Cuts at: 4628 5640 4628
Size: 1012 5556
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3505 3817 794
Size: 2711 312 3545
Fragments arranged by size:
3545 2711 312
BstBI TT'CG_AA
Cuts at: 2709 5372 2709
Size: 2663 3905
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6568
BstXI CCAn_nnnn'nTGG
Cuts at: 2986 3859 2986
Size: 873 5695
BstYI r'GATC_y
Cuts at: 1972 2082 2535 3238 3510 3579 3898 4265 4650
Size: 110 453 703 272 69 319 367 385
Cuts at: 4650 4667 6119 6205 6217 6563 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 703 453 385 367 346 319
272 110 86 69 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2178 2511 2697 2808 3036 3336
Size: 552 939 174 333 186 111 228 300
Cuts at: 3336 513
Size: 3745
Fragments arranged by size:
3745 939 552 333 300 228 186 174
111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2787 487
Size: 683 56 1561 4268
Fragments arranged by size:
4268 1561 683 56
BtsI GCAGTG_nn'
Cuts at: 2389 3510 4084 4878 4898 2389
Size: 1121 574 794 20 4059
Fragments arranged by size:
4059 1121 794 574 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6568
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6533
DraI TTT'AAA
Cuts at: 2131 3872 2131
Size: 1741 4827
DraIII CAC_nnn'GTG
Cuts at: 2365 3808 2365
Size: 1443 5125
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2423 3605 5575 809
Size: 507 1107 1182 1970 1802
Fragments arranged by size:
1970 1802 1182 1107 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2211 2694
Size: 54 1425 50 275 18 225 156 483
Cuts at: 2694 3333 3471 3593 3849 4015 4904 6533 8
Size: 639 138 122 256 166 889 1629 43
Fragments arranged by size:
1629 1425 889 639 483 275 256 225
166 156 138 122 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2694 4015 1537
Size: 275 882 1321 4090
Fragments arranged by size:
4090 1321 882 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3686 4498 1353
Size: 2333 812 3423
Fragments arranged by size:
3423 2333 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2683 3248 3355 5155 5529 5675
Size: 1064 138 1094 565 107 1800 374 146
Cuts at: 5675 387
Size: 1280
Fragments arranged by size:
1800 1280 1094 1064 565 374 146 138
107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2318 2380 2891 2945 3068 3197
Size: 393 593 93 62 511 54 123 129
Cuts at: 3197 3765 3806 4631 5228 6015 6283 1239
Size: 568 41 825 597 787 268 1524
Fragments arranged by size:
1524 825 787 597 593 568 511 393
268 129 123 93 62 54 41
EcoNI CCTnn'n_nnAGG
Cuts at: 3683 3683
Size: 6568
EcoO109I rG'GnC_Cy
Cuts at: 2200 2242 2542 2659 2902 3541 2200
Size: 42 300 117 243 639 5227
Fragments arranged by size:
5227 639 300 243 117 42
EcoRI G'AATT_C
Cuts at: 2432 3877 4255 2432
Size: 1445 378 4745
Fragments arranged by size:
4745 1445 378
FseI GG_CCGG'CC
Cuts at: 3657 3657
Size: 6568
FspI TGC'GCA
Cuts at: 5074 5074
Size: 6568
HaeII r_GCGC'y
Cuts at: 1678 2064 3558 5715 1678
Size: 386 1494 2157 2531
Fragments arranged by size:
2531 2157 1494 386
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2833 2865 5209
Size: 32 135 32 563 32 666 32 2344
Cuts at: 5209 5241 5283 5315 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2344 666 563 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3912 4158 669
Size: 988 281 1974 246 3079
Fragments arranged by size:
3079 1974 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6568
HpaI GTT'AAC
Cuts at: 4158 4158
Size: 6568
KpnI G_GTAC'C
Cuts at: 3893 3893
Size: 6568
MfeI C'AATT_G
Cuts at: 4167 4167
Size: 6568
MluI A'CGCG_T
Cuts at: 6444 6444
Size: 6568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3508 5682 5866 1305
Size: 2203 2174 184 2007
Fragments arranged by size:
2203 2174 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 2213 3335 3595 3851
Size: 54 1425 343 225 156 1122 260 256
Cuts at: 3851 10
Size: 2727
Fragments arranged by size:
2727 1425 1122 343 260 256 225 156
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2273 2558 3144 3734 3857 4526 4885
Size: 648 1107 285 586 590 123 669 359
Cuts at: 4885 5044 518
Size: 159 2042
Fragments arranged by size:
2042 1107 669 648 590 586 359 285
159 123
MspA1I CmG'CkG
Cuts at: 1606 2413 2502 2605 2626 2699 3984 4661 5809
Size: 807 89 103 21 73 1285 677 1148
Cuts at: 5809 6054 1606
Size: 245 2120
Fragments arranged by size:
2120 1285 1148 807 677 245 103 89
73 21
NaeI GCC'GGC
Cuts at: 3655 3655
Size: 6568
NcoI C'CATG_G
Cuts at: 513 1065 2004 2178 2511 3036 513
Size: 552 939 174 333 525 4045
Fragments arranged by size:
4045 939 552 525 333 174
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6568
NgoMIV G'CCGG_C
Cuts at: 3653 3653
Size: 6568
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6568
NotI GC'GGCC_GC
Cuts at: 4015 4015
Size: 6568
NruI TCG'CGA
Cuts at: 2381 2381
Size: 6568
NspI r_CATG'y
Cuts at: 1132 1229 2557 3733 3926 5471 1132
Size: 97 1328 1176 193 1545 2229
Fragments arranged by size:
2229 1545 1328 1176 193 97
PasI CC'CwG_GG
Cuts at: 1348 2545 1348
Size: 1197 5371
PciI A'CATG_T
Cuts at: 5467 5467
Size: 6568
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2413 2545 1108
Size: 1305 132 5131
Fragments arranged by size:
5131 1305 132
PfoI T'CCnGG_A
Cuts at: 2868 3045 3246 3678 3834 2868
Size: 177 201 432 156 5602
Fragments arranged by size:
5602 432 201 177 156
PmeI GTTT'AAAC
Cuts at: 3872 3872
Size: 6568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4637 4669 6150 6182 4637
Size: 32 1481 32 5023
Fragments arranged by size:
5023 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2659 3541 2659
Size: 882 5686
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6568
PsiI TTA'TAA
Cuts at: 4138 4138
Size: 6568
PspOMI G'GGCC_C
Cuts at: 2241 2241
Size: 6568
PstI C_TGCA'G
Cuts at: 830 3920 830
Size: 3090 3478
PvuI CG_AT'CG
Cuts at: 2001 2274 2385 2001
Size: 273 111 6184
Fragments arranged by size:
6184 273 111
PvuII CAG'CTG
Cuts at: 2413 2626 2413
Size: 213 6355
RsrII CG'GwC_CG
Cuts at: 3494 3494
Size: 6568
SacII CC_GC'GG
Cuts at: 2700 2700
Size: 6568
SalI G'TCGA_C
Cuts at: 1936 3910 1936
Size: 1974 4594
SanDI GG'GwC_CC
Cuts at: 3541 3541
Size: 6568
SbfI CC_TGCA'GG
Cuts at: 3920 3920
Size: 6568
ScaI AGT'ACT
Cuts at: 4816 6528 4816
Size: 1712 4856
SexAI A'CCwGG_T
Cuts at: 3804 3804
Size: 6568
SfcI C'TryA_G
Cuts at: 826 1045 2326 3916 5051 5732 5923 826
Size: 219 1281 1590 1135 681 191 1471
Fragments arranged by size:
1590 1471 1281 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6568
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6568
SmaI CCC'GGG
Cuts at: 1872 3895 1872
Size: 2023 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4672 5573 5835 820
Size: 197 926 2729 901 262 1553
Fragments arranged by size:
2729 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4359 493
Size: 3866 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6568
SphI G_CATG'C
Cuts at: 1132 2557 3926 1132
Size: 1425 1369 3774
Fragments arranged by size:
3774 1425 1369
SspI AAT'ATT
Cuts at: 5 52 4492 5
Size: 47 4440 2081
Fragments arranged by size:
4440 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6568
StyI C'CwwG_G
Cuts at: 513 1065 2004 2178 2511 2898 3036 4007 513
Size: 552 939 174 333 387 138 971 3074
Fragments arranged by size:
3074 971 939 552 387 333 174 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4595 4612 4765 4950 5395 325
Size: 1255 3015 17 153 185 445 1498
Fragments arranged by size:
3015 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4814 6526 96
Size: 274 80 33 51 1443 2837 1712 138
Fragments arranged by size:
2837 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6528 6560 6528
Size: 32 6536
Tth111I GACn'n_nGTC
Cuts at: 1316 2252 3605 1316
Size: 936 1353 4279
Fragments arranged by size:
4279 1353 936
XbaI T'CTAG_A
Cuts at: 3904 3904
Size: 6568
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3043 3043
Size: 6568
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6568
XmaI C'CCGG_G
Cuts at: 1870 3893 1870
Size: 2023 4545
XmnI GAAnn'nnTTC
Cuts at: 2612 2930 4697 2612
Size: 318 1767 4483
Fragments arranged by size:
4483 1767 318
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4376 276
Size: 53 83 186 936 2842 2468
Fragments arranged by size:
2842 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I
Bsu36I EcoICRI EcoRV FalI FspAI KasI NarI NsiI
PacI PmlI PspXI PsrI SacI SapI SfiI SfoI
SrfI SwaI